Effect of Two Substrate Types on Feeding Efficiency by Juvenile Chinese Sturgeon Acipenser sinensis on Barcheek Goby Rhinogobius giurinus

We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby Rhinogobius giurinus was not affected by sand vs. smooth glass substrate. Gobies are among the most common prey eaten by wild juvenile A. sinensis in the estuary. Test results found neither substrate type significantly affected feeding efficiency by juveniles. Previous research found a strong innate habitat preference of A. sinensis for sand substrate. The present results indicate that the preference for sand is not related to efficiently capturing R. giurinus on sand, but is an adaptation predisposing juvenile A. sinensis to seek sandy substrate where R. giurinus and other benthic forage are the most abundant in the Yangtze Estuary.

Systematic analysis of feeding behaviors and their effects on feed efficiency in Pekin ducks

Background： Feeding behavior study is important for animal husbandry and production. However, few studies were conducted on the feeding behavior and their relationship with feeding efficiency in Pekin ducks. In order to investigate the feeding behavior and their relationship with feed efficiency and other economic traits in Pekin ducks, we selected 358 male Pekin ducks and recorded feeding information between 3 to 6 wk of age using automatic electronic feeders, and compared the feeding behavior under different residual feed intake（RFI） levels.Results： We observed that total feed time, daily feed intake and feed intake per meal had strong positive correlations with feed efficiency traits; moreover, strong correlation between feed intake per meal and body weight was found（R=0.32, 0.36）. Daily feeding rate meal and meal duration had weak correlations with feed efficiency（R=0.14~0.15）. The phenotypic correlation of between-meal pauses, with feed efficiency was not observed. When daily changes were analyzed, high RFI ducks had the highest feed consumption over all times, and obvious differences in daily visits were found among different RFI level animals during the middle period; these differences were magnified with age, but there was no difference in daily meal number. Moreover, our data indicate that high RFI birds mainly take their meals at the edge of the population enclosure, where they are more susceptible to environmental interference.Conclusions： Overal, this study suggests that the general feeding behaviors can be accurately measured using automatic electronic feeders and certain feeding behaviors in Pekin ducks are associated with improved feed efficiency.

Identification of biomarkers associated with the feed efficiency by metabolomics profiling:results from the broiler lines divergent for high or low abdominal fat content

Background:Improving feed efficiency(FE)is one of the main objectives in broiler breeding.It is difficult to directly measure FE traits,and breeders hence have been trying to identify biomarkers for the indirect selection and improvement of FE traits.Metabolome is the"bridge"between genome and phenome.The metabolites may potentially account for more of the phenotypic variation and can suitably serve as biomarkers for selecting FE traits.This study aimed to identify plasma metabolite markers for selecting high-FE broilers.A total of 441 birds from Northeast Agricultural University broiler lines divergently selected for abdominal fat content were used to analyze plasma metabolome and estimate the genetic parameters of differentially expressed metabolites.Results:The results identified 124 differentially expressed plasma metabolites(P<0.05)between the lean line(highFE birds)and the fat line(low-FE birds).Among these differentially expressed plasma metabolites,44 were found to have higher positive or negative genetic correlations with FE traits(|r_g|≥0.30).Of these 44 metabolites,14 were found to display moderate to high heritability estimates(h~2≥0.20).However,among the 14 metabolites,4 metabolites whose physiological functions have not been reported were excluded.Ultimately,10 metabolites were suggested to serve as the potential biomarkers for breeding the high-FE broilers.Based on the physiological functions of these metabolites,reducing inflammatory and improving immunity were proposed to improve FE and increase production efficiency.Conclusions:According to the pipeline for the selection of the metabolite markers established in this study,it was suggested that 10 metabolites including 7-ketocholesterol,dimethyl sulfone,epsilon-(gamma-glutamyl)-lysine,gamma-glutamyltyrosine,2-oxoadipic acid,L-homoarginine,testosterone,adenosine 5'-monophosphate,adrenic acid,and calcitriol could be used as the potential biomarkers for breeding the"food-saving broilers".

Agolin Ruminant®, an Essential Oil Blend, Increases Energy-Corrected Milk and Feed Efficiency in a High Component Dairy Herd

Agolin® Ruminant (Agolin) is a blend of essential oils developed to improve feed efficiency while at the same time reducing rumen enteric methane production. Studies have shown that the product improves lactational performance, but the range of results has been varied. This experiment evaluated the effects of the feed additive Agolinon milk production in a high-component (fat and protein) dairy herd when provided for an extended time. The experiment was conducted at a large commercial dairy in the Pacific Northwest, USA. Eight pens of cows (350 to 500 cows/pen) were blocked by production, and pens within blocks were randomly assigned to treatment. All pens received a common total mixed ration. Treatment consisted of providing 1 g/cow/day of Agolin to the test group. A 4-week adaptation period was followed by an 11-week study period. Milk production and milk composition were determined by cow by averaging daily performance for the one-week period before the start of the trial (covariate period) and the last week of each of the two test periods. Feed intakes were determined by pen at the same time. Milk yield was 1.11 kg/cow/day greater (P < 0.001) at the midpoint and 1.48 kg/cow/day greater (P < 0.001) at the end of the test period for cows receiving the feed additive. Similarly, protein yields were 0.03 (P < 0.001) and 0.07 kg/cow/day greater (P < 0.001) at the midpoint and end of the feeding period. Milk fat yield was not different (P = 0.854) between treatment groups at the midpoint of the trial but increased with treatment (P = 0.002) by the end of the trial. Energy-corrected milk/feed ratio, based on pen parameters, was significantly altered by treatment at the final test period (1.48 vs. 1.64 kg/kg for control and test treatments, respectively).

A review of feed efficiency in swine:biology and application

Feed efficiency represents the cumulative efficiency with which the pig utilizes dietary nutrients for maintenance, lean gain and lipid accretion. It is closely linked with energy metabolism, as the oxidation of carbon-containing components in the feed drive all metabolic processes. While much is known about nutrient utilization and tissue metabolism, blending these subjects into a discussion on feed efficiency has proven to be difficult. For example, while increasing dietary energy concentration will almost certainly increase feed efficiency, the correlation between dietary energy concentration and feed efficiency is surprisingly low. This is likely due to the plethora of non-dietary factors that impact feed efficiency, such as the environment and health as well as individual variation in maintenance requirements, body composition and body weight. Nonetheless, a deeper understanding of feed efficiency is critical at many levels. To individual farms, it impacts profitability. To the pork industry, it represents its competitive position against other protein sources. To food economists, it means less demand on global feed resources. There are environmental and other societal implications as well. Interestingly, feed efficiency is not always reported simply as a ratio of body weight gain to feed consumed. This review will explain why this arithmetic calculation, as simple as it initially seems, and as universally applied as it is in science and commerce, can often be misleading due to errors inherent in recording of both weight gain and feed intake. This review discusses the importance of feed efficiency, the manner in which it can be measured and reported, its basis in biology and approaches to its improvement. It concludes with a summary of findings and recommendations for future efforts.

New insights into the associations among feed efficiency, metabolizable efficiency traits and related QTL regions in broiler chickens

Background: Improving the feed efficiency would increase profitability for producers while also reducing the environmental footprint of livestock production. This study was conducted to investigate the relationships among feed efficiency traits and metabolizable efficiency traits in 180 male broilers. Significant loci and genes affecting the metabolizable efficiency traits were explored with an imputation-based genome-wide association study. The traits measured or calculated comprised three growth traits, five feed efficiency related traits, and nine metabolizable efficiency traits.Results: The residual feed intake(RFI) showed moderate to high and positive phenotypic correlations with eight other traits measured, including average daily feed intake(ADFI), dry excreta weight(DEW), gross energy excretion(GEE), crude protein excretion(CPE), metabolizable dry matter(MDM), nitrogen corrected apparent metabolizable energy(AMEn), abdominal fat weight(Ab F), and percentage of abdominal fat(Ab P). Greater correlations were observed between growth traits and the feed conversion ratio(FCR) than RFI. In addition, the RFI, FCR, ADFI, DEW,GEE, CPE, MDM, AMEn, Ab F, and Ab P were lower in low-RFI birds than high-RFI birds(P < 0.01 or P < 0.05), whereas the coefficients of MDM and MCP of low-RFI birds were greater than those of high-RFI birds(P < 0.01). Five narrow QTLs for metabolizable efficiency traits were detected, including one 82.46-kb region for DEW and GEE on Gallus gallus chromosome(GGA) 26, one 120.13-kb region for MDM and AMEn on GGA1, one 691.25-kb region for the coefficients of MDM and AMEn on GGA5, one region for the coefficients of MDM and MCP on GGA2(103.45–103.53 Mb), and one 690.50-kb region for the coefficient of MCP on GGA14. Linkage disequilibrium(LD) analysis indicated that the five regions contained high LD blocks, as well as the genes chromosome 26 C6 orf106 homolog(C26 H6 orf106), LOC396098, SH3 and multiple ankyrin repeat domains 2(SHANK2), ETS homologous factor(EHF), and histamine receptor H3-like(HRH3 L), which are known to be involved in the regulation of neurodevelopment, cell proliferation and differentiation, and food intake.Conclusions: Selection for low RFI significantly decreased chicken feed intake, excreta output, and abdominal fat deposition, and increased nutrient digestibility without changing the weight gain. Five novel QTL regions involved in the control of metabolizable efficiency in chickens were identified. These results, combined through nutritional and genetic approaches, should facilitate novel insights into improving feed efficiency in poultry and other species.

Body composition and compensatory growth in Nile tilapia Oreochromis niloticus under different feeding intervals

We investigated the growth and body composition of Nile tilapia under five different feeding regimes. A control group was fed to satiation twice daily for 185 days; four treatment groups were fed at intervals of 2, 3, 4 or 7 days(dietary ‘restricted' period, days 0–80) and then fed to satiation(‘refeeding' period, days 80–185). Compensatory growth in weight and length of the feed-restricted groups was observed during the refeeding period. However, the growth of none of the restricted groups caught up with that of the control group over the experimental period. Feed intake upon refeeding increased with the duration of deprivation. There were no significant differences in feed efficiency between the restricted and control groups during the refeeding stage, suggesting that hyperphagia was the mechanism responsible for the increased growth rates during this period. Tilapia preferentially used n-3 polyunsaturated fatty acids and nonessential amino acids during the restricted-feeding period. Higher production was achieved by higher feed consumption. We suggest that if attainment of market size in minimum time is required, fish should be consistently fed to satiation, while taking care to avoid the possible negative consequences of overfeeding.

A Method of Drawing Upseleciton Index for Improving Feed Efficiency

Based on the beneficial trait which is from the two component traits of feed efficiency by price, a method for drawing up selection index to improve feed efficiency is discussed. The method avoids some troubles from directly selecting feed efficiency and settles the questions to draw up selection index for the kind of ratio traits simultaneously.

Identification of gut microbes associated with feed efficiency by daily-phase feeding strategy in growing-finishing pigs

Feed efficiency is one of the most important issues for sustainable pig production.Daily-phase feeding(DPF)is a form of precision feeding that could improve feed efficiency in pigs.Gut microbiota can regulate host nutrient digestion,absorption,and metabolism.However,which key microbes may play a vital role in improving the feed efficiency during DPF remains unclear.In the present study,we used a DPF program compared to a three-phase feeding(TPF)program in growing-finishing pigs to investigate the effects of gut microbiota on feed efficiency.A total of 204 Landrace×Yorkshire pigs(75 d)were randomly assigned into 2 treatments.Each treatment was replicated 8 times with 13 to 15 pigs per replicate pen.Pigs in the TPF group were fed with a commercial feeding program that supplied fixed feed for phasesⅠ,Ⅱ,andⅢ,starting at 81,101,and 132 d of age,respectively,and pigs in the DPF group were fed a blend of adjacent phase feed from 81 to 155 d at a gradual daily ratio and phaseⅢfeed from 155 to 180 d of age.Daily feed intake and body weight were recorded by a computerized device in the feeders.Feces and blood samples were collected from 1 pig per replicate at 155 and 180 d of age.The results showed that the DPF program remarkably improved the feed efficiency at 155 d(P<0.001)and 180 d of age(P<0.001),with a significant reduction of the intake of crude protein(P<0.01),net energy(P<0.001),crude fiber(P<0.001),ether extract(P<0.01),and ash(P<0.001).The daily-phase feeding program increased the abundance of Prevotella copri(P<0.05)and Paraprevotella clara(P<0.05),while it decreased the abundance of Ocilibacter(P<0.05)at 155 d of age.The results of correlation analysis indicated that the differentially abundant microbiota communities were closely associated with 20 metabolites which enriched amino acid and phenylalanine metabolism.Our results suggest that 2 key microbes may contribute to feed efficiency during daily-phase feeding strategies in pigs.

Functional Genes in Relation to Residual Feed Intake in Murrah Buffalo Heifers

High Feed efficiency (FE) in growing heifers has economic importance in dairy, but remains less understood in buffaloes. Feed conversion efficiency is defined as dry matter intake (DMI) per unit body weight gain and is determined as residual feed intake (RFI), i.e., the difference between actual and predicted feed intake to gain unit body weight during a feed trial run for 78 days under control feeding. A large variation was identified ranging between -0.42 to 0.35 in growing buffalo heifers (n = 40) of age between 11 to 15 months. An average daily weight gain (ADG) varied between 382.0 and 807.6 g/day when compared with the control-fed heifers at an organized buffalo farm. The whole blood transcriptome data obtained from the selected growing heifers from extremes of estimated high and low RFI efficiency were compared with the reference assembly generated from the transcriptome of multiparous buffaloes (n = 16) of diverse age of maturity, period of regaining post partum cyclicity and level of milk production. Differentially expressed genes (DEGs) were identified using the reference genome of Mediterranean water buffalo. GO: terms (Padj 0.05, FDR 0.05) enriched by annotated DEGs and biological pathways in gene network for RFI efficiency trait were identified. GO: terms specific to pre-transcriptional regulation of nucleus and Chromatin organization under Nucleoplasm, Energy balancing, Immunity, Cell signaling, ROS optimization, ATP generation through the Electron Transport chain and cell proliferation were determined. The study reveals the indicators targeting the actual metabolic changes and molecular functions underlying the feed utilization capacity of buffaloes. Estimated RFI efficiency revealed a large variation over heifers which may lower the DMI even up to 13.6% thus, enabling an increase in ADG up to 16% by involving efficient heifers in breeding plan. The study revealed a scope of high gain by selective breeding for FE in heifers. FE variants catalogued in the study are useful breed-specific RFI markers for future reference. The study contributes to the understanding of feed efficiency in buffaloes and its association with key interactive traits such as reproduction and growth. This knowledge can be utilized to develop more effective breeding programs.

Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in latephase laying hens

Background:Dietary essential oil(EO)supplementation can exert favorable effects on gut health in broilers.However,it is unknown whether EO could improve intestinal functions,consequently beneficial for egg performance and quality in late-phase laying hens.This study was aimed to investigate the potential effects of EO on production performance,egg quality,intestinal health and ileal microbiota of hens in the late phase of production.A total of 28860-week-old Hy-line Brown laying hens were randomly divided into 4 groups and fed a basal diet(control)or basal diets supplemented with oregano EO at 100,200 and 400 mg/kg(EO100,EO200 and EO400).Results:Dietary EO supplementation resulted in a quadratic decrease(P<0.05)in feed conversion ratio with lower(P<0.05)feed conversion ratio in EO200 group than the control during weeks 9–12 and 1–12 of the trial.Compared to the control,EO addition resulted in higher(P<0.05)eggshell thickness at the end of week.4,8 and 12 and higher(P<0.05)chymotrypsin activity.There was a quadratic elevation(P<0.05)in ileal chymotrypsin and lipase activity,along with a linear increase in villus height to crypt depth ratio.Quadratic declines(P<0.05)in mRNA expression of IL-1β,TNF-α,IFN-γ and TLR-4,concurrent with a linear and quadratic increase(P<0.05)in ZO-1 expression were identified in the ileum with EO addition.These favorable effects were maximized at medium dosage(200 mg/kg)of EO addition and intestinal microbial composition in the control and EO200 groups were assessed.Dietary EO addition increased(P<0.05)the abundances of Burkholderiales,Actinobacteria,Bifidobacteriales,Enterococcaceae and Bacillaceae,whereas decreased Shigella abundance in the ileum.Conclusions:Dietary EO addition could enhance digestive enzyme activity,improve gut morphology,epithelial barrier functions and modulate mucosal immune status by altering microbial composition,thus favoring feed efficiency and eggshell quality of late-phase laying hens.

Linkage between the intestinal microbiota and residual feed intake in broiler chickens

Background:Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on feed efficiency of livestock animals.However,the intestinal microbes that are critically involved in feed efficiency remain elusive.Methods:To identify intestinal bacteria associated with residual feed intake(RFI)in chickens,male Cobb broiler chicks were individually housed from day 14 to day 35.Individual RFI values were calculated for 56 chickens.Luminal contents were collected from the ileum,cecum,and cloaca of each animal on day 35.Bacterial DNA was isolated and subjected to 16S rRNA gene sequencing.Intestinal microbiota was classified to the feature level using Deblur and QIIME 2.High and low RFI groups were formed by selecting 15 and 17 chickens with the most extreme RFI values for subsequent LEfSe comparison of the difference in the microbiota.Spearman correlation analysis was further performed to identify correlations between the intestinal microbiota composition and RFI.Results:No significant difference in evenness,richness,and overall diversity of the microbiota in the ileum,cecum,or cloaca was observed between high and low RFI chickens.However,LEfSe analysis revealed a number of bacterial features being differentially enriched in either high or low RFI chickens.Spearman correlation analysis further identified many differentially enriched bacterial features to be significantly correlated with RFI(P<0.05).Importantly,not all short-chain fatty acid(SCFA)producers showed a positive association with RFI.While two novel members of Oscillibacter and Butyricicoccus were more abundant in low-RFI,high-efficiency chickens,several other SCFA producers such as Subdoligranulum variabile and two related Peptostreptococcaceae members were negatively associated with feed efficiency.Moreover,a few closely-related Lachnospiraceae family members showed a positive correlation with feed efficiency,while others of the same family displayed an opposite relationship.Conclusions:Our results highlight the complexity of the intestinal microbiota and a need to differentiate the bacteria to the species,subspecies,and even strain levels in order to reveal their true association with feed efficiency.Identification of RFI-associated bacteria provides important leads to manipulate the intestinal microbiota for improving production efficiency,profitability,and sustainability of poultry production.

Residual feed intake divergence during the preweaning period is associated with unique hindgut microbiome and metabolome profiles in neonatal Holstein heifer calves

Background:Recent studies underscored that divergence in residual feed intake(RFI)in mature beef and dairy cattle is associated with changes in ruminal microbiome and metabolome profiles which may contribute,at least in part,to better feed efficiency.Because the rumen in neonatal calves during the preweaning period is underdeveloped until close to weaning,they rely on hindgut microbial fermentation to breakdown undigested diet components.This leads to production of key metabolites such as volatile fatty acids(VFA),amino acids,and vitamins that could potentially be absorbed in the hind-gut and help drive growth and development.Whether RFI divergence in neonatal calves is associated with changes in hindgut microbial communities and metabolites is largely unknown.Therefore,the objective of the current study was to determine differences in hindgut microbiome and metabolome in neonatal Holstein heifer calves retrospectively-grouped based on feed efficiency as mostefficient(M-eff)or least-efficient(L-eff)calves using RFI divergence during the preweaning period.Methods:Twenty-six Holstein heifer calves received 3.8 L of first-milking colostrum from their respective dams within 6 h after birth.Calves were housed in individual outdoor hutches bedded with straw,fed twice daily with a milk replacer,and had ad libitum access to a starter grain mix from birth to weaning at 42 d of age.Calves were classified into M-eff[n=13;RFI coefficient=−5.72±0.94 kg DMI(milk replacer+starter grain)/d]and L-eff[n=13;RFI coefficient=5.61±0.94 kg DMI(milk replacer+starter grain)/d]based on a linear regression model including the combined starter grain mix and milk replacer DMI,average daily gain(ADG),and metabolic body weight(MBW).A deep sterile rectal swab exposed only to the rectum was collected immediately at birth before colostrum feeding(i.e.,d 0),and fecal samples at d 14,28,and 42(prior to weaning)for microbiome and untargeted metabolome analyses using 16S rRNA gene sequencing and LC-MS.Microbiome data were analyzed with the QIIME 2 platform and metabolome data with the MetaboAnalyst 4.0 pipeline.Results:No differences(P>0.05)in body measurements including body weight(BW),body length(BL),hip height(HH),hip width(HW),and wither height(WH)were detected between M-eff and L-eff calves at birth and during preweaning.Although milk replacer intake did not differ between groups,compared with L-eff,M-eff heifers had lower starter intake(P<0.01)between d 18 to 42 of age,whereas no differences(P>0.05)for ADG,cumulative BWG,or body measurements were observed between RFI groups during the preweaning period.Microbiome and metabolome profiles through the first 42 d of age indicated greater hindgut capacity for the production of energy-generating substrates(butyrate and propionate)and essential nutrients(vitamins and amino acids)in heifers with greater estimated feed efficiency.Conclusion:Despite consuming approximately 54.6%less solid feed(cumulative intake,10.90 vs.19.98±1.66 kg)from birth to weaning,the microbiome-metabolome changes in the hindgut of most-efficient heifers might have helped them maintain the same level of growth as the least-efficient heifers.

Application of omics technologies for a deeper insight into quali-quantitative production traits in broiler chickens:A review

The poultry industry is continuously facing substantial and different challenges such as the increasing cost of feed ingredients, the European Union’s ban of antibiotic as growth promoters, the antimicrobial resistance and the high incidence of muscle myopathies and breast meat abnormalities. In the last decade, there has been an extraordinary development of many genomic techniques able to describe global variation of genes, proteins and metabolites expression level. Proper application of these cutting-edge omics technologies(mainly transcriptomics, proteomics and metabolomics) paves the possibility to understand much useful information about the biological processes and pathways behind different complex traits of chickens. The current review aimed to highlight some important knowledge achieved through the application of omics technologies and proteo-genomics data in the field of feed efficiency, nutrition, meat quality and disease resistance in broiler chickens.

REDUCED GROWTH IN HYBRID TILAPIA (OREOCHROMIS MOSSAMBICUS×O. NILOTICUS) AT INTERMEDIATE STOCKING DENSITY

Hybrid tilapia were reared at densities of 1, 5 or 10 fish per tank for four weeks. Mortality was 0 at 1 and 10 fish per tank, but was 25% at 5 fish per tank. Specific growth rate was highest at 1 fish per tank, and lowest at 5 fish per tank. The lower growth rate at the intermediate stocking density was associated with reduced feed efficiency, but there was no reduction in feed intake or digestibility. The results suggested increased metabolic cost caused by aggressive behaviour at intermediate stocking density, which can be suppressed by a further increase in density.

Influence of the Feed Gas Composition on the Fischer-Tropsch Synthesis in Commercial Operations

Key technical challenges relating to the Fischer-Tropsch （F-T） synthesis applied in the commercialization of coal/gas-to-liquids （CTL/GTL） technologies have been reviewed. Based on the experiences accumulated from pilot plant, semi-work test and lab researches, the influences of the H2/CO ratio and the CO2 in the feed gas on the F-T process as well as on CTL/GTL complex in terms of product yields, energy efficiency and carbon utilization efficiency have been studied. Being contrary to the current design schemes for F-T process using the coal derived syngas and the iron-based cata lyst, it is suggested to feed the F-T synthesis unit with a syngas having a H2/CO ratio of 0.5 and then adjusting to 1.4 via the recycling process. As a result, the carbon efficiency of the whole plant could be reached to as high as 50%. For the issue of CO2 addition to the feed gas, it is proved that only a diluting role is played under the current commercial slurry phase F-T process.

Evaluation of Agolin^®, an Essential Oil Blend, as a Feed Additive for High Producing Cows

There is a growing demand for feed additives that can not only reduce dairy enteric methane emissions but also increase milk production and feed efficiency. Just one product is currently commercially available which accomplishes both of these goals. The purpose of this study was to confirm the performance benefits of the product (Agolin?, Agolin SA, Biere, Switzerland) in high producing mid-lactation dairy cows under United States feeding conditions. Four matched pens of approximately 150 mid-lactation cows/pen and averaging over 50 kg of milk/cow/day were enrolled in a side-by-side study. All pens received a common total mixed diet ad libitum, and the essential oil blend was administered via a concentrated farm pack to provide 1 g/cow/day to cows in the 2 test pens. Milk weights were determined, and samples were collected for compositional analysis over the last 2 days of the pretrial (May 11 and 12, 2020) and end of the trial (July 18 and 19, 2020) periods. Dry matter intake was measured by pen daily for the last 10 days of each feeding period. Milk fat and milk protein yields were greater (P < 0.05) for cows receiving added Agolin. There was a tendency (P = 0.06) for energy corrected milk/dry matter intake to be greater for cows receiving the Agolin (1.88) relative to the control diet (1.76). The trial showed that Agolin assisted in improving production parameters of economic importance to dairy producers.

Effects of Spent Craft Brewers’ Yeast on Fermentation and Methane Production by Rumen Microorganisms

Saccharomyces cerevisiae is a key component of beer brewing and a major by-product. The leftover, spent brewers’ yeast from large breweries has been used as a protein supplement in cattle;however the possible advantages of spent yeast from smaller craft breweries, containing much higher levels of bioactive hop acids, have not been evaluated. Hops secondary metabolites from the hops (Humulus lupulus L.) used to make beer are concentrated in the yeast during brewing, and have antimicrobial activity against Gram-positive bacteria. Uncultivated suspensions of bovine rumen microorganisms produced less methane during fructose fermentation when exposed to inactivated, and freeze-dried spent craft brewers’ yeast than a bakers’ yeast control. The experiment was repeated with caprine rumen microorganisms and ground grass hay as the substrate. Likewise, in the presence of craft brewers’ yeast less methane was produced (2.7% vs. 6.9% CH4). Both experiments also revealed a decrease in acetic acid production, but not propionic acid production, when craft brewers’ yeast was included. These results indicated that spent yeast could represent a co-product for craft breweries, and a feed supplement for ruminants that has a favorable impact on methane production.

Dietary effects of A zolla pinnata combined with exogenous digestive enzyme(Digestin^(TM)) on growth and nutrients utilization of freshwater prawn, Macrobrachium rosenbergii(de Man 1879)

The present study was conducted to evaluate the effect of either individual or combined wheat bran(WB) replacement with Azolla pinnata supplemented with Digestin^(TM) in the diet of freshwater prawn, Macrobrachium rosenbergii Postlarvae(PL) on growth performance, nutrient utilization, chemical body composition and survival(%). Experimental diets were a wheat bran-soybean based diet with no Azolla and Digestin^(TM)(control, T_1), and diets containing 17% Azolla supplemented with Digestin^(TM) 0%(T_2), 1%(T_3), 2%(T_4) and 3%(T 5). Each experimental diet was allocated into three tanks(6 m^3/tank) fed for 12 wks. Each tank was subdivided into three equal pens by nets(2 m^3) and stoked with 84 PL/m^2. The experimental diets were readily consumed by prawns PLs where both high growth and good feed efficiency were achieved for all diets. The results showed that the diets containing A. pinnata supplemented with Digestin? at the level up to 3% have the higher growth and better nutrient utilization than the control diet. No differences were observed for moisture and protein content among the experimental diets. However, the highest protein content was observed on prawns fed on diets T_1 and T 5 respectively, while the lowest value was recorded for T_4 diet. The results also show that prawn PLs fed the diets contain A. pinnata and supplemented with Digestin^(TM) recorded the highest values of body lipid content compared to the control diet. Feed efficiency and economic conversion rate(ECR) values show that economic performance and the cost-effectiveness of the A. pinnata supplemented with up to 3% Digestin^(TM) recorded the highest net return, and therefore it is recommended for prawn, M. rosenbergii PL's. These results are clearly indicating that A. pinnata have a good potential for use in prawn diets at reasonable levels than other conventional diets.

Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge

Background： When an animal is exposed to a stressor, metabolic rate, energy consumption and utilisation increase primarily through activation of the hypothalamic-pituitary-adrenal（HPA） axis. Changes to partitioning of energy by an animal are likely to influence the efficiency with which it is utilised. Therefore, this study aimed to determine the physiological stress response to an exogenous adrenocorticotropic hormone（ACTH） challenge in beef heifers divergently ranked on phenotypic residual feed intake（RFI）.Results： Data were collected on 34 Simmental weaning beef heifers the progeny of a well characterized and divergently bred RFI suckler beef herd. Residual feed intake was determined on each animal during the postweaning stage over a 91-day feed intake measurement period during which they were individually offered adlibitum grass silage and 2 kg of concentrate per head once daily. The 12 highest [0.34 kg DM/d] and 12 lowest[-0.48 kg DM/d] ranking animals on RFI were selected for use in this study. For the physiological stress challenge heifers（mean age 605 ± 13 d; mean BW 518 ± 31.4 kg） were fitted aseptically with indwelling jugular catheters to facilitate intensive blood collection. The response of the adrenal cortex to a standardised dose of ACTH（1.98 IU/kg metabolic BW^（0.75）） was examined. Serial blood samples were analysed for plasma cortisol, ACTH and haematology variables. Heifers differing in RFI did not differ（P = 0.59） in ACTH concentrations. Concentration of ACTH peaked（P 〈 0.001） in both RFI groups at 20 min post-ACTH administration, following which concentration declined to baseline levels by 150 min. Similarly, cortisol systemic profile peaked at 60 min and concentrations remained continuously elevated for 150 min. A RFI × time interaction was detected for cortisol concentrations（P = 0.06） with high RFI heifers had a greater cortisol response than Low RFI from 40 min to 150 min relative to ACTH administration. Cortisol response was positively associated with RFI status（r = 0.32; P 〈 0.01）. No effect of RFI was evident for neutrophil, lymphocytes, monocyte, eosinophils and basophil count. Plasma red blood cell number（6.07 vs. 6.23; P = 0.02） and hematocrit percentage（23.2 vs. 24.5; P = 0.02） were greater for low than high RFI animals.Conclusions： Evidence is provided that feed efficiency is associated with HPA axis function and susceptibility to stress, and responsiveness of the HPA axis is likely to contribute to appreciable variation in the efficiency feed utilisation of cattle.