Electroacupuncture at Feishu(BL13) and Zusanli(ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease

Inflammation and lung function decline are the main pathophysiological features of chronic obstructive pulmonary disease （COPD）. Acupuncture can improve lung function in patients with COPD, but the underlying mechanisms are not well understood. Orexins （OXs）, which are found in peripheral plasma, are neuropeptides that regulate respiration and their levels are related to COPD. Therefore, we hypothesized that acupuncture might alter OXs, reduce lung inflammation and improve lung function in COPD. METHODS： COPD was induced in rats by exposure to cigarette smoke for 8 weeks and injecting with lipopolysaccharide twice. Electroacupuncture （EA） was performed at Feishu （BL13） and Zusanli （ST36） for 30 min/d for 2 weeks. Rat lung function and morphology were assessed after EA. The levels of tumor necrosis factor-α （TNF-a） and intedeukin-1β （IL-16） in bronchoalveolar lavage fluid （BALF） and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. OX receptor mRNA levels and immunopositive cells were assessed with real-time polymerase chain reaction and immunohistochemical methods, respectively. The relationships among lung function, cell factors, and OX levels were analyzed by Pearson correlation analyses. RESULTS： Compared with the control group, lung function was significantly decreased in the rats with COPD （P〈0.05）. There were obvious increases in TNF-α and IL-1β levels in BALF （P〈0.05 and P〈0.01, respectively）, orexin A level in lung tissue （P〈0.01; but not orexin B） and mRNA expressions of OX 1 type receptor （OXR1） and OX 2 type receptor （OXR2） in lung tissue （P〈0.05 and P〈0.01, respectively）; the integrative optical densities （IODs） of both receptors were greater in the COPD group （P〈0.05）. For rats with COPD subjected to EA, lung function was improved （P〈0.05）. There were notable decreases in TNF-a and IL-1β levels （P〈0.05 and 〈0.01, respectively） in BALF. Orexin A, but not orexin B, levels in lung tissue also decreased （P〈0.01）, as did mRNA expression of OXlR and OX2R in lung tissue （P〈0.05 and P〈0.01, respectively）. Receptor IODs were also reduced after EA treatment （P〈0.05）. Furthermore, orexin A levels and ratio of forced expiratory volume in 0.3 s to forced vital capacity were strongly negatively correlated （P〈0.01）, and orexin A was positively correlated with TNF-α and IL-1β （P〈0.001 and P〈0.05, respectively）.CONCLUSION： EA at Zusanli and Feishu improved lung function of rats with COPD and had an anti-inflammatory effect, which may be related to down-regulation of OXA and its receptors.

Effect of scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) on neutrophil-to-lymphocyte ratio and quality of life in patients with non-small-cell lung cancer: A randomized,controlled trial

OBJECTIVE: To evaluate the effect of heat stimulation via scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) on the neutrophil-to-lymphocyte ratio(NLR) and quality of life in patients with non-small-cell lung cancer(NSCLC).METHODS: Seventy patients with NSCLC were randomly assigned into two groups: group A received scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) every day for 6 weeks, while group B received no intervention(control group). Outcome measures were the NLR and the scores from the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire(EORTC QLQ-C30). The NLR and the EORTC QLQ-C30 were assessed at baseline and at the end of 6 weeks.RESULTS: Five participants dropped out, leaving afinal total of 65 participants who completed the trial. Groups A and B had a similar mean NLR at baseline. After the treatment course, the NLR in group A was significantly lower than that in group B(P <0.001). Compared with group B, the EORTC QLQ-C30 scores in group A were significantly greater in terms of global health status or quality of life(P < 0.001) and function(P < 0.05), and significantly lower in terms of symptoms(P < 0.05).CONCLUSION: The present study suggests that performing scar-producing moxibustion by heat-stimulating the acupoints Zusanli(ST 36) and Feishu(BL13) effectively decreases the NLR and improves the quality of life in patients with NSCLC.

Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV14) on transdermal uptake of sinapine thiocyanate in asthma gel

OBJECTIVE: To investigate the effect of stimulating the acupoints Feishu(BL 13) and Dazhui(GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.METHODS: Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu(BL 13) acupoint group, the Dazhui(GV 14) acupoint group, and the nonacupoint group or control group. Using micro-dialysis technology, preprocessed skin probes were implanted into the rats at Feishu(BL 13), Dazhui(GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu(BL 13), Dazhui(GV14) acupoints, and the nonacupoint for all groups.Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.RESULTS: The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics(r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.CONCLUSION: Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.

Effects of moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure

OBJECTIVE:To observe the effects of moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril on myocardial cells apoptosis index,the expression levels of apoptosis-related proteins cytochrome c(Cyt-C)and apoptosis-inducing factor(AIF)in chronic heart failure(CHF)rats.METHODS:Sixty-five rats were randomly divided into normal group(n=10)and model-I group(n=55).After modeling,CHF rats in model-I group were divided into model group,moxibustion group,benazepril group,moxibustion plus benazepril group(abbreviated as aibei group,the same below),10 rats in each group.Echocardiogram index was examined by echocardiography.Hemodynamic indices were measured by rat cardiac function meter.Serum B-type brain natriuretic peptide(BNP)was detected by enzymelinked immunosorbent assay.Myocardial cells apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling staining.Pathological changes of myocardial tissues were observed by hematoxylin and eosin staining.The expression levels of Cyt-C and AIF in myocardial tissues were detected by Western blot.RESULTS:Compared with normal group,ejection fraction and left ventricular diameter shortening rate in model-Ⅰgroup were significantly reduced,myocardial cells of rats in model group exhibited unclear transverse striations,cells swellings and vacuoles,cardiac functions were deteriorated,serum BNP level,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were significantly increased.Compared with model group,myocardial cells of rats in moxibustion group,benazepril group,and aibei group were dyed more evenly,muscle fibers were arranged relatively neatly,cardiac functions were improved,serum BNP level,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were significantly decreased.Compared with aibei group,cardiac functions were worsened,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were increased.CONCLUSION:Moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril could improve CHF better than moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)or benazepril alone.The mechanisms might be that they can inhibit the expressions of Cyt-C and AIF,and inhibit the apoptosis of cardiomyocytes.

微波治疗仪照射辅助治疗支气管肺炎的临床效果及对患儿症状消失时间的影响

目的:探究微波治疗仪照射辅助治疗支气管肺炎的临床效果及对患儿症状消失时间的影响。方法:选择2021年6月—2022年12月遵义市妇幼保健院收治的50例支气管肺炎患儿,按随机数字表法分为两组。对照组(n=25)给予常规治疗,观察组(n=25)给予常规治疗和微波治疗仪照射辅助治疗。对比两组症状和啰音消失时间、临床疗效、血清炎症因子水平和血常规指标。结果:观察组总有效率高于对照组,差异有统计学意义(P<0.05);观察组发热、喘息、咳嗽、啰音消失时间均短于对照组,差异均有统计学意义(P<0.05);治疗后,两组超敏C反应蛋白水平均低于治疗前,且观察组低于对照组,差异均有统计学意义(P<0.05);治疗后,观察组中性粒细胞、白细胞计数均低于对照组,淋巴细胞高于对照组,差异均有统计学意义(P<0.05)。结论:微波治疗仪照射辅助治疗支气管肺炎患儿的效果确切,有助于改善血常规指标,缓解炎症反应,有效促进临床症状缓解。

Changes of the microcirculatory blood perfusion at “Feishu”( BL 13) in the COPD rats

Objective To observe the change of the specificity of the microcirculatory blood peifusion at the area of "Feishu"(BL 13) in the rats of chronic obstructive pulmonary disease (COPD). Methods According to the random number table, 60 Wistar rats were divided into a 29d model No.1 group (C1 group).

针刺对哮喘模型大鼠肺组织α-平滑肌肌动蛋白及纤维连接蛋白表达水平的影响

目的观察针刺对哮喘模型大鼠肺组织α-平滑肌肌动蛋白(α-SMA)、纤维连接蛋白(FN)表达水平的影响.方法选择雄性SPF级SD大鼠40只,按随机掷骰子法将大鼠分为正常对照组、模型组、药物组、针刺组,每组10只.于实验1d、8d,正常对照组腹腔注射1 mL 0.9%氯化钠溶液,模型组腹腔注射1 mL 10%复合卵蛋白溶液致敏.实验15 d,正常对照组雾化喷入0.9%氯化钠溶液20 min,模型组雾化喷入1%卵蛋白溶液20 min激发哮喘,制模成功后正常对照组与模型组不进行处理,药物组腹腔注射0.05%地塞米松磷酸钠溶液,每日1次,持续10d;针刺组针刺肺俞、大椎、风门穴,每2d施针1次,共7次.比较4组大鼠肺组织中信号转导和转录激活因子6(STAT6)mRNA表达、转化生长因子-β1(TGF-β1)的阳性表达和蛋白表达及α-SMA、FN、外周血T淋巴细胞亚群(CD3^(+)、CD4^(+)、CD8^(+))水平的差异.结果与正常对照组比较,模型组STAT6 mRNA表达、TGF-β1蛋白表达和TGF-β1、α-SMA、FN阳性表达及外周血中T淋巴细胞群(CD3^(+)、CD8^(+))均明显升高[STAT6 mRNA表达(2^(-ΔΔCt)):13.12±2.20比1.02±0.20,TGF-β1蛋白表达(A值):0.10±0.01比0.06±0.01,TGF-β1阳性表达(A值):0.11±0.01比0.08±0.01,α-SMA阳性表达(A值):19.65±1.37比7.74±0.81,FN阳性表达(A值):18.40±0.79比6.50±0.60,CD3^(+):0.70±0.03比0.59±0.02,CD8^(+):0.39±0.02比0.20±0.02,均P<0.05],CD4^(+)明显降低(0.32±0.02比0.39±0.03,P<0.05);药物组和针刺组STAT6 mRNA表达、TGF-β1蛋白表达和TGF-β1、α-SMA、FN阳性表达及外周血中T淋巴细胞群(CD3^(+)、CD8^(+))水平均较模型组明显降低,CD4^(+)较模型组明显升高,且以针刺组的变化较药物组更显著[STAT6 mRNA表达(2-ΔΔCt):3.01±0.55比5.64±0.65,TGF-β1蛋白表达(A值):0.06±0.01比0.09±0.01,TGF-β1阳性表达(A值):0.08±0.01比0.09±0.01,α-SMA阳性表达(A值):12.33±0.50比14.99±0.53,FN阳性表达(A值):9.91±0.61比13.19±0.66,CD3^(+):0.60±0.03比0.65±0.04,CD4^(+):0.39±0.02比0.35±0.02,CD8^(+):0.21±0.04比0.28±0.03,均P<0.05].结论对于哮喘模型大鼠,针刺其肺俞、大椎、风门穴可以刺激肺组织的神经和肌肉,促进肺部血液循环,增强肺功能,改善肺组织的健康状况,降低肺组织中α-SMA、FN的阳性表达,改善气道重塑,效果显著.

艾灸配合药物治疗气虚血瘀型慢性心力衰竭的疗效观察

目的基于“心肺同治”理论观察艾灸心俞和肺俞配合药物治疗气虚血瘀型慢性心力衰竭的临床疗效。方法将60例气虚血瘀型慢性心力衰竭患者随机分为观察组和对照组,每组30例。对照组采用常规西药治疗,观察组在对照组基础上采用艾灸心俞和肺俞治疗。观察两组治疗前后心功能指标[左心室射血分数(left ventricular ejection fraction,LVEF)和心率(heart rate,HR)]及血清炎性因子指标[C反应蛋白(C-reactive protein,CRP)和髓过氧化物酶(myeloperoxidase,MPO)]的变化情况,并比较两组临床疗效及中医证候疗效。结果两组治疗后LVEF较同组治疗前显著上升,HR及MPO、CRP水平显著下降,差异均具有统计学意义(P<0.05)。观察组治疗后各项心功能指标及炎性因子指标与对照组比较,差异均具有统计学意义(P<0.05)。观察组治疗后临床总有效率及中医证候总有效率分别为90.0%和96.7%,明显高于对照组的66.7%和73.3%,差异均具有统计学意义(P<0.05)。结论艾灸配合药物治疗气虚血瘀型慢性心力衰竭疗效确切,能提高患者的心功能,减轻炎症反应。

培元养心温针灸治疗咳嗽变异性哮喘临床疗效观察

【目的】观察培元养心理论指导下温针灸治疗咳嗽变异性哮喘(cough variant asthma,CVA)患者的临床疗效。【方法】将70例CVA患者随机分为治疗组与对照组,每组各35例。对照组给予布地奈德福莫特罗吸入粉雾剂(信必可都保)治疗,治疗组给予培元养心温针灸治疗(其中,肺俞、心俞、膏肓俞、脾俞、肾俞、关元、足三里温针灸,天突、膻中、神门常规针刺),疗程为8周并随访3个月。观察2组患者治疗前、治疗后及随访时的改良简易咳嗽评价量表(CET)评分和焦虑自评量表(SAS)评分的变化情况,比较2组患者的临床疗效、复发率和不良反应情况。【结果】(1)治疗8周后,治疗组的临床控制率和总有效率分别为22.86%(8/35)、97.14%(34/35),对照组分别为2.86%(1/35)、88.57%(31/35),治疗组的临床控制率和总有效率均高于对照组,其中,2组的临床控制率比较差异有统计学意义(P<0.05),而总有效率比较差异无统计学意义(P>0.05)。(2)治疗后和随访时,2组患者的改良CET评分及治疗组的SAS评分均较治疗前明显降低(P<0.01),且治疗组在治疗后和随访时对改良CET评分及SAS评分的降低作用均明显优于对照组,差异均有统计学意义(P<0.01)。(3)治疗后3个月随访时,治疗组的复发率为5.88%(2/34),明显低于对照组的29.03%(9/31),差异有统计学意义(P<0.05)。(4)治疗过程中,治疗组无不良反应发生,对照组有4例(11.42%)患者出现轻度不适症状,2组患者的不良反应发生率比较,差异无统计学意义(P>0.05)。【结论】培元养心理论指导下的温针灸疗法可更好地改善CVA患者的咳嗽症状及焦虑情绪,且远期疗效更好,复发率低于西药治疗的对照组。

《针灸大成》中肺俞穴临床应用浅析

目的:通过研究《针灸大成》中肺俞穴的相关条文,总结其定位归经、刺法灸法、临床应用以及配穴规律,探讨肺俞穴的临床应用规律。方法:运用中医文献学的研究方法,整理《针灸大成》中有关肺俞穴的记载,从肺俞穴的定位归经、刺法灸法、临床应用、配穴规律等方面进行讨论分析和归纳总结。结果:肺俞穴位于第3胸椎棘突下,后正中线旁开1.5寸,属足太阳膀胱经,为肺经背俞穴,可针、可灸;临床多广泛用于肺系、诸虚劳损、脾胃、肢体经络以及皮肤病;组方上多配穴使用,常配伍足太阳膀胱经、任脉、足阳明胃经、手太阴肺经及手厥阴心包经腧穴。结论:归纳总结《针灸大成》中肺俞穴的定位归经、刺灸法及临床应用规律,为现代针灸临床应用提供理论依据。

《武威汉代医简》所载“肺腧”穴研究

通过释读《武威汉代医简》相关简文,分析其所载“肺腧”定位的表述方式,并结合传世文献,探寻其具体定位,梳理其演变脉络。《武威汉代医简》所载“肺腧”与后世的厥阴俞在定位、主治方面十分契合,可补全厥阴俞演变进程的缺失环节,故《武威汉代医简》所载“肺腧”应即为后世厥阴俞。且通过对比古今厥阴俞主治功效,参考现代临床报道,厥阴俞的功效主治应还存在进一步研究与补充的空间,比如可用于初步探查病变脏器及治疗肝胆病所致胀满。

针刺肺俞穴及非经非穴点对肺功能影响的对照研究

目的 :探讨针刺肺俞穴及非经非穴点对肺功能的影响。方法 :选健康男性学员 80名 ,年龄2 0～ 2 2岁之间 ,随机分为两组 ,应用美制 2 12 0肺功能仪分别于针刺前 10min及针刺后 10min测试FVC、FEV1 0 、FEV1 0 %、MMF、PEFR、·V75、·V50 、·V2 5。结果 :针刺肺俞穴后FVC明显增高 (P <0 0 5 ) ,针刺非经非穴点后 ,各项指标虽有不同程度的提高 ,但无统计学意义 (P >0 0 5 )。 结论 :针刺肺俞穴可提高健康人的FVC ,而针刺非经非穴点则无此作用。

茶碱微乳经皮吸收及药代动力学研究

目的:研究茶碱微乳在家兔不同皮肤部位给药后的经皮吸收及药代动力学。方法:采用HPLC测定血浆中茶碱的浓度,应用基于Guass-Newton-Zhu原理设计的NLFTZ计算机程序对实验数据进行解析。结果:茶碱微乳经背部肺腧穴给药后的血药浓度高于非穴位给药,有极显著性差异(P<0.01);茶碱经皮吸收符合两个独立的具不同时滞的零级吸收、一级消除的一室模型迭加形成的复合模型,血药浓度平稳,可以维持24 h以上。结论:茶碱微乳经皮吸收均具有双通道、双过程复合的特征;穴位经皮给药的两种通道中存在特殊的加速通道,而药物经穴位周围皮肤的穿透表现为慢吸收过程。

针刺与艾灸对健康人肺功能的影响

目的 探讨针刺、艾灸肺俞穴后对肺功能的影响 .方法 选健康男性学员 40例 ,年龄 2 0～ 2 2岁之间 ,应用美制2 12 0肺功能仪分别于针刺前 10 min及针刺后 10 min,艾灸前10 min及艾灸后 10 min.测试用力肺活量 (FVC) ,用力呼气量 (FEV1) ,最大呼气中段流量 (MMF) ,最大呼气流速 (PE-FR )和 75 % ,5 0 %和 2 5 %肺活量时的最大呼气流速 (V75 ,V5 0和 V2 5 ) .结果 针刺及艾灸肺俞穴后 FVC明显增高 (P<0 .0 5 ) ,其余各项指标针刺及艾灸前后无明显变化 (P>0 .0 5 ) ,且两种治疗方法之间无显著性差异 (P>0 .0 5 ) .结论 针刺或艾灸背部的肺俞穴 ,可提高健康人的 FVC。

艾灸“肺俞、膏肓俞”对BLMA5所致肺纤维化大鼠肺上皮细胞E-cad基因表达的实验研究

目的:探究艾灸"肺俞、膏肓俞"抑制肺纤维化进程的作用机制。方法 :将81只8周龄SPF级大鼠随机分9组:空白对照组;模型组(7 d组、11 d组、23 d组、35 d组);艾灸组(11 d组、23 d组、35 d组);阳性对照(泼尼松)组,每组9只。采用气管内一次性注入博来霉素复制肺纤维化模型。各组造模均于第二天进行治疗及干预。艾灸组交替艾灸双侧肺俞和膏育俞,3壮/穴,5 mg/壮,1次/d,10次/疗程。泼尼松组以泼尼松灌胃。空白组和所有模型组与艾灸组采取同样的抓取和固定,不给予任何治疗。共治疗3个疗程,疗程间休息2 d。各组在设定的时间窗口处死动物,采用定量PCR法检测E-cad基因的表达水平。结果:艾灸治疗组E-cad基因表达量比同期模型组高,尤以第35 d明显。艾灸35 d组(0.32±0.07)明显高于模型35 d组(0.10±0.04)(P<0.01)。艾灸组和泼尼松组无明显差异,统计学无意义(P>0.05)。结论:艾灸"肺俞、膏肓俞"可抑制肺纤维化的进程,其内在机制可能与升高肺上皮细胞E-cad基因表达有关。

艾灸“肺俞”“膏肓”影响BLM_(A5)诱导肺纤维化大鼠TGF-β1表达实验研究

目的:探索针灸阻抑肺纤维化的效应机制,为针灸介入肺纤维化防治提供实验依据。方法:将SD大鼠140只随机分为4组:空白组、模型组、艾灸组、泼尼松组,每组35只。气管内注入博莱霉素制作大鼠肺纤维化模型,造模后7d开始治疗,以5mg艾绒灸其双侧“肺俞”“膏肓”,每穴3壮,每天1次,10d为一疗程,共治疗3个疗程后处死动物,采用PCR检测其转化生长因子β1(TGFβ1)mRNA表达。结果:肺组织TGFβ1mRNA表达分别为:空白组1.0258±0.0057,模型组2.8104±0.2905,艾灸组1.6174±0.1136,泼尼松组1.7176±0.1079。艾灸组、泼尼松组与模型组比较,P<0.01,艾灸组与泼尼松组比较,P>0.05。结论:艾灸“肺俞”“膏肓”与泼尼松治疗均能显著抑制肺纤维化大鼠肺组织TGFβ1mRNA表达。

肺、脾、肾俞拔罐治疗对支原体肺炎患儿免疫功能影响及临床疗效观察

目的:观察肺、脾、肾俞拔罐治疗对支原体肺炎患儿免疫功能的影响及其临床疗效。方法:将60例患儿随机分为两组,对照组采用常规西医抗感染、止咳化痰及雾化吸入治疗,治疗组在对照组的基础上佐以肺、脾、肾俞拔罐疗法,比较两组患儿在咳嗽消失时间及肺部啰音消失时间上有无差异,以及该疗法对患儿免疫功能的影响。结果:体液免疫指标Ig A、Ig G在两组患儿治疗前后变化无统计学差异;Ig M在治疗组中疗前、疗后变化有统计学意义(P<0.05)。细胞免疫指标:CD3+表达比例在两组患儿治疗前后变化无统计学差异;CD3+CD4+、CD3+CD8+、CD4+/CD8+在治疗组中疗前、疗后变化有统计学意义(P<0.05)。炎性指标hs-CRP在两组患儿治疗前后变化均有统计学差异(P<0.05)。临床评价指标:咳嗽消失时间和肺部啰音消失时间在两组患儿中存在显著差异(P<0.05)。结论:肺、脾、肾俞拔罐佐治小儿支原体肺炎能明显缩短疗程,改善患儿免疫功能,值得推广。

针刺肺俞及四花穴对慢性支气管炎迁延期老年患者免疫球蛋白的影响

目的观察针刺肺俞及四花(膈俞和胆俞)穴对慢性支气管炎迁延期老年患者免疫球蛋白的影响。方法将60例老年慢性支气管炎迁延期患者随机分为治疗组和对照组,每组30例。两组均采用常规药物治疗,治疗组采用针刺肺俞及四花穴治疗,对照组采用口服蜜炼川贝枇杷膏治疗。分别观察两组治疗前后免疫球蛋白变化。结果治疗组治疗后免疫球蛋白各项指标(IgA、IgM、IgG)与同组治疗前比较,差异均具有统计学意义(P<0.05)。治疗组治疗后免疫球蛋白各项指标与对照组比较,差异均具有统计学意义(P<0.05)。结论针刺肺俞及四花穴对慢性支气管炎迁延期老年患者免疫球蛋白具有升高作用。

咳喘穴位敷贴对哮喘大鼠肺组织JAK1、STAT6表达的影响

目的观察咳喘穴位敷贴对慢性支气管哮喘大鼠肺组织JAK1、STAT6表达的影响。方法将40只SD大鼠随机分为对照组、哮喘模型组、咳喘穴位敷贴组、地塞米松组,每组10只。除对照组外,其余各组采用卵清蛋白致敏并激发的方法制备慢性支气管哮喘大鼠模型。造模成功后,咳喘穴位敷贴组大鼠相应穴位贴敷咳喘穴位敷贴进行干预治疗,地塞米松组大鼠腹腔注射地塞米松磷酸钠注射液0.5 mg/(kg·d)。治疗14 d后取大鼠肺组织,HE染色观察肺组织形态学变化,免疫组织化学、Real time PCR检测肺组织JAK1和STAT6蛋白及mRNA的表达水平。结果与对照组相比,哮喘模型组大鼠肺组织可见大量炎性细胞浸润,气道内有大量分泌物,肺泡结构紊乱;与哮喘模型组相比,咳喘穴位敷贴组和地塞米松组大鼠肺组织炎性细胞浸润减少、气道分泌物减少、肺泡排列规则。与对照组相比,哮喘模型组大鼠气道上皮JAK1、STAT6蛋白和mRNA表达明显升高(P<0.01);与哮喘模型组相比,咳喘穴位敷贴组和地塞米松组大鼠气道上皮JAK1、STAT6蛋白表达下调(P<0.05或P<0.01),JAK1、STAT6 mRNA表达下调(P<0.01)。结论咳喘穴位敷贴能够改善支气管哮喘大鼠支气管及肺组织炎症,其机制可能与降低JAK1、STAT6 mRNA和蛋白表达有关。

艾灸肺俞、心俞治疗慢性心衰临床观察

目的观察艾灸配合西药治疗慢性心衰的临床疗效。方法将60例患者随机分为对照组和治疗组,每组30例。对照组给予西药常规治疗;治疗组在西药常规治疗的基础上,采用艾条温和灸双侧肺俞、心俞穴,每穴灸治20 min,每日1次,共灸治4星期。比较两组治疗后临床疗效和心功能相关指标。结果对照组总有效率为76.7%,治疗组总有效率为90.0%,治疗组优于对照组(P<0.05);两组治疗后HR、CO、LVEF较治疗前有显著改善(P<0.01),治疗组治疗后HR、CO、LVEF与对照组比较有显著差异(P<0.01),治疗组治疗后HR、CO、LVEF改善状况优于对照组。结论艾灸配合西药治疗慢性心衰临床疗效优于单纯西药治疗。