The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the releva...The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the relevant authorities have carried out the activities of stock enhancement and releasing.It can increase biomass and recover resources.However,compared with increasing biomass,there were still few reports on its effect on the recovery of resources.Resource recovery is a process related to whether the released individuals can form a reproductive population.Up to now,there has been a lack of evidence whether the released F.chinensis can complete the entire life history,and form reproduction population.In this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow Sea.After identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture samples.It was confirmed for the first time that at least part of the released F.chinensis can complete overwintering and reproductive migration,and maintain the migration habits as their wild counterparts.Therefore,we infered that the released shrimp can reproduce under natural conditions,these F.chinensis can form reproductive populations theoretically if without human intervention.These results indicated that enhancenment and release activities have a positive effect on resource recovery.展开更多
Glutathione S-transferases(GSTs)are a superfamily of multifunction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates.In t...Glutathione S-transferases(GSTs)are a superfamily of multifunction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates.In the present study,a delta class GST gene(designated as FcδGST)was cloned from Fenneropenaeus chinensis using rapid amplification of c DNA ends(RACE)technology.The complete cDNA sequence of FcδGST was 780 bp in length,which includes a 27-bp 5′non-coding region(UTR),a 117-bp 3′UTR,a 636-bp open reading frame(ORF),and a polyadenylate signal site(AATAAA)presented at the upstream of poly A tail.The FcδGST gene encoded 211 amino acids peptide,including a GST_N domain and a GST_C domain,and exhibited high similarity with previously reported delta GSTs.The predicted molecular mass of FcδGST protein was 23.39 kDa,and its theoretical isoelectric point(pI)was 5.34.The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues,with the highest expression level in hemocytes and hepatopancreas.During the stimulation of Vibrio anguillarum or white spot syndrome virus(WSSV),the m RNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation.The purified recombinant FcδGST protein(designated as rFcδGST)exhibited specific catalytic activity against 1-chloro-2,4-dinitrobenzene(CDNB)substrate with relatively low stable enzymatic activities.These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of F.chinensis.展开更多
The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV(white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the p...The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV(white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifesta-tions of hemocytes were similar among moribund shrimps infected via injection,feeding and by nature. Firstly,the total hemocyte counts(THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly,necrotic,broken and dis-integrated cells were often observed,and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly,necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared,whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV,and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.展开更多
根据2011年及2013年6个航次的调查,结合历史资料,统计分析了莱州湾中国对虾(Fenneropenaeus chinensis)、三疣梭子蟹(Portunus trituberculatus)及二者敌害生物单位捕捞努力量渔获量(Catch per unit fishing effort,CPUE)的长期变化,研...根据2011年及2013年6个航次的调查,结合历史资料,统计分析了莱州湾中国对虾(Fenneropenaeus chinensis)、三疣梭子蟹(Portunus trituberculatus)及二者敌害生物单位捕捞努力量渔获量(Catch per unit fishing effort,CPUE)的长期变化,研究了目前二者的饵料生物密度以及饵料生物与敌害生物的数量分布。结果显示,目前中国对虾、三疣梭子蟹以及二者敌害生物的CPUE均呈历史低位水平。放流期间,莱州湾浮游植物饵料等级为Ⅴ级(很丰富),底栖动物饵料等级为Ⅳ级(丰富);中国对虾的饵料生物密度分别以莱州湾东北部(5月)和中东部(6月)最高,敌害生物密度分别以中西部(5月)和东南部(6月)最高;三疣梭子蟹的饵料生物密度均以莱州湾西南部和东北部最高,敌害生物密度分别以中西部(5月)和西北部(6月)最高。展开更多
The effects of various salinity fluctuation amplitudes (2,4,6 and 8) on the growth,osmolarity,Na+-K+-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studi...The effects of various salinity fluctuation amplitudes (2,4,6 and 8) on the growth,osmolarity,Na+-K+-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied.The results show that weight gain in the salinity fluctuation treatments was better than that in control;in particular,the weight gain of treatments S4 and S0,at 231.8% and 196.3%,respectively,was significantly different (P<0.05).The hemolymph osmolarity of treatments S0,S2,S4,S6 and S8 was 635.4,630.8,623.6,614.4 and 600.3 mOsm/kg,respectively,and decreased with increasing salinity fluctuation amplitude.The level of Na+-K+-ATPase activity in gills of F.chinensis was higher than that in hepatopancreas,but there were no significant differences among all treatments,either in gills or hepatopancreas (P>0.05).The relative level of Hsp70 in treatment S4 was 48.4% and 40.4% higher than control in muscle and eyestalks,respectively,with the highest values being recorded under a salinity fluctuation amplitude of 4.展开更多
中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days po...中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days post-larva,PL16)即开始雌雄分化,此时,雌虾第4与第5对步足间腹甲处的锥突出现明显下陷,而雄虾的没有下陷。PL54时,雌虾纳精囊瓣膜出现,然后继续发育,到PL124时形成纳精囊雏形;雄虾交接器发育较晚,到PL54时,雄虾第1泳足的内肢才出现分化,在PL106基本形成雄性交接器。展开更多
[Objective] The aim was to estimate the developmental characteristics of Huanghai No.1 and the first generation of wild population in Fenneropenaeus chinensis for providing a reference for the development, feeding man...[Objective] The aim was to estimate the developmental characteristics of Huanghai No.1 and the first generation of wild population in Fenneropenaeus chinensis for providing a reference for the development, feeding management and breeding. [Method] Four growth models were used to fit the growth patterns of 15 morphological traits. The squared multiple correlation coefficient (R2 ) of the Cubic growth model was higher than the other three models, so it was selected to investigate the growth pattern and age in month at inflexion. [Result] Ages in month at inflexion of body weight in Huanghai No.1 and the G1 population were 2.87 (body weight at inflexion was 14.98 g) and 4.05 (body weight at inflexion was 26.26 g), respectively. In addition, Ages in month at inflexion of morphological characters in Huanghai No.1 were from 0.51 to 3.07. CL had the most rapid growth rate, followed by AW, CH, AH, CW, BL, FL, AL5, AL3, AL4, TL, AL2, AL1, and AL6. Ages in month at inflexion in the G 1 population were from 2.38 to 3.08, except that of AL2, and the order of achieving the most rapid growth rate was AL2, AL1, AL3, AL4, CH, AL5, CW, FL, AH, AW, TL, CL, BL, and AL6, that delayed one month than that of Huanghai No.1 except AL2 and AL1. [Conclusion] The development of Huanghai No.1 cultivar was advanced for about one month compared to that of the G1 population.展开更多
基金Laoshan Laboratory under contract No.LSKJ202203803。
文摘The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the relevant authorities have carried out the activities of stock enhancement and releasing.It can increase biomass and recover resources.However,compared with increasing biomass,there were still few reports on its effect on the recovery of resources.Resource recovery is a process related to whether the released individuals can form a reproductive population.Up to now,there has been a lack of evidence whether the released F.chinensis can complete the entire life history,and form reproduction population.In this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow Sea.After identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture samples.It was confirmed for the first time that at least part of the released F.chinensis can complete overwintering and reproductive migration,and maintain the migration habits as their wild counterparts.Therefore,we infered that the released shrimp can reproduce under natural conditions,these F.chinensis can form reproductive populations theoretically if without human intervention.These results indicated that enhancenment and release activities have a positive effect on resource recovery.
基金Supported by the Project of Sanya Yazhou Bay Science and Technology City Management Foundation (No.SKJC-KJ-2019KY01)the Startup Fund of Young Talents Project of Ocean University of China。
文摘Glutathione S-transferases(GSTs)are a superfamily of multifunction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates.In the present study,a delta class GST gene(designated as FcδGST)was cloned from Fenneropenaeus chinensis using rapid amplification of c DNA ends(RACE)technology.The complete cDNA sequence of FcδGST was 780 bp in length,which includes a 27-bp 5′non-coding region(UTR),a 117-bp 3′UTR,a 636-bp open reading frame(ORF),and a polyadenylate signal site(AATAAA)presented at the upstream of poly A tail.The FcδGST gene encoded 211 amino acids peptide,including a GST_N domain and a GST_C domain,and exhibited high similarity with previously reported delta GSTs.The predicted molecular mass of FcδGST protein was 23.39 kDa,and its theoretical isoelectric point(pI)was 5.34.The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues,with the highest expression level in hemocytes and hepatopancreas.During the stimulation of Vibrio anguillarum or white spot syndrome virus(WSSV),the m RNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation.The purified recombinant FcδGST protein(designated as rFcδGST)exhibited specific catalytic activity against 1-chloro-2,4-dinitrobenzene(CDNB)substrate with relatively low stable enzymatic activities.These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of F.chinensis.
文摘The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV(white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifesta-tions of hemocytes were similar among moribund shrimps infected via injection,feeding and by nature. Firstly,the total hemocyte counts(THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly,necrotic,broken and dis-integrated cells were often observed,and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly,necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared,whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV,and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.
文摘根据2011年及2013年6个航次的调查,结合历史资料,统计分析了莱州湾中国对虾(Fenneropenaeus chinensis)、三疣梭子蟹(Portunus trituberculatus)及二者敌害生物单位捕捞努力量渔获量(Catch per unit fishing effort,CPUE)的长期变化,研究了目前二者的饵料生物密度以及饵料生物与敌害生物的数量分布。结果显示,目前中国对虾、三疣梭子蟹以及二者敌害生物的CPUE均呈历史低位水平。放流期间,莱州湾浮游植物饵料等级为Ⅴ级(很丰富),底栖动物饵料等级为Ⅳ级(丰富);中国对虾的饵料生物密度分别以莱州湾东北部(5月)和中东部(6月)最高,敌害生物密度分别以中西部(5月)和东南部(6月)最高;三疣梭子蟹的饵料生物密度均以莱州湾西南部和东北部最高,敌害生物密度分别以中西部(5月)和西北部(6月)最高。
基金Supported by the National Natural Science Foundation of China (No. 30571441)Reward Research Foundation for Talented Young and Middle Aged Scientists of Shandong,China (No. 2006BS07002)National Key Technology Research and Development Program of China (No. 2006BAD09A07)
文摘The effects of various salinity fluctuation amplitudes (2,4,6 and 8) on the growth,osmolarity,Na+-K+-ATPase activity and Hsp70 of juvenile Fenneropenaeus chinensis cultured in seawater with a salinity of 20 were studied.The results show that weight gain in the salinity fluctuation treatments was better than that in control;in particular,the weight gain of treatments S4 and S0,at 231.8% and 196.3%,respectively,was significantly different (P<0.05).The hemolymph osmolarity of treatments S0,S2,S4,S6 and S8 was 635.4,630.8,623.6,614.4 and 600.3 mOsm/kg,respectively,and decreased with increasing salinity fluctuation amplitude.The level of Na+-K+-ATPase activity in gills of F.chinensis was higher than that in hepatopancreas,but there were no significant differences among all treatments,either in gills or hepatopancreas (P>0.05).The relative level of Hsp70 in treatment S4 was 48.4% and 40.4% higher than control in muscle and eyestalks,respectively,with the highest values being recorded under a salinity fluctuation amplitude of 4.
文摘中国明对虾(Fenneropenaeus chinensis)的性别分化和生殖器官发育是其繁殖生物学的重要部分,也是进行性别调控的基础。经过2年的取样观察,研究了中国明对虾雌性纳精囊和雄性交接器的分化与发育过程。结果显示,早在仔虾后16 d(16 days post-larva,PL16)即开始雌雄分化,此时,雌虾第4与第5对步足间腹甲处的锥突出现明显下陷,而雄虾的没有下陷。PL54时,雌虾纳精囊瓣膜出现,然后继续发育,到PL124时形成纳精囊雏形;雄虾交接器发育较晚,到PL54时,雄虾第1泳足的内肢才出现分化,在PL106基本形成雄性交接器。
基金Supported by grants from China Agriculture Research System(CARS-47)the National Natural Science Foundation of China(3117240130901101)
文摘[Objective] The aim was to estimate the developmental characteristics of Huanghai No.1 and the first generation of wild population in Fenneropenaeus chinensis for providing a reference for the development, feeding management and breeding. [Method] Four growth models were used to fit the growth patterns of 15 morphological traits. The squared multiple correlation coefficient (R2 ) of the Cubic growth model was higher than the other three models, so it was selected to investigate the growth pattern and age in month at inflexion. [Result] Ages in month at inflexion of body weight in Huanghai No.1 and the G1 population were 2.87 (body weight at inflexion was 14.98 g) and 4.05 (body weight at inflexion was 26.26 g), respectively. In addition, Ages in month at inflexion of morphological characters in Huanghai No.1 were from 0.51 to 3.07. CL had the most rapid growth rate, followed by AW, CH, AH, CW, BL, FL, AL5, AL3, AL4, TL, AL2, AL1, and AL6. Ages in month at inflexion in the G 1 population were from 2.38 to 3.08, except that of AL2, and the order of achieving the most rapid growth rate was AL2, AL1, AL3, AL4, CH, AL5, CW, FL, AH, AW, TL, CL, BL, and AL6, that delayed one month than that of Huanghai No.1 except AL2 and AL1. [Conclusion] The development of Huanghai No.1 cultivar was advanced for about one month compared to that of the G1 population.
