莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝...莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝色的荧光。实验证明,莲幼胚子叶细胞随着发育时期的增长,造粉质体 DNA 的含量逐渐增加,显示出有规律的动态变化过程。在电镜下观察,造粉质体 DNA 区域外无膜结构存在,故具有原核生物的特征。这种质体 DNA 的纤维丝直径大约为25。展开更多
The effect of apatite ranoparticles on proliferation potential and biological behaviour of the human hepatocellular carcinoma in vitro were investigated. After the treatment of Bel- 7402 hepatoceUalar carcinoma cells ...The effect of apatite ranoparticles on proliferation potential and biological behaviour of the human hepatocellular carcinoma in vitro were investigated. After the treatment of Bel- 7402 hepatoceUalar carcinoma cells with apatite nanoparticles at a concentration of 5 × 10^-4 mmol/ L for 4days, Feulgen and AgNOR stain were conducted and the .specimens were observed by microscope. The DNA and AgNOR were quantified with image analysis techniques. It was found that there was a signiftcant decrease of the DNA content （ 58.62 ± 6.52 ） in the nanoparticles treated group compared to the control （ 78.21 ± 4.17 ）. It was further found that there was a decrease in the number of AgNOR granules in the nanoparticle treated group （7.41 ± 1.02） compared to the control group （ 9.95 ± 0.28 ）. The experimental results showed thnt apatite nanoparticles could decrease the DNA reproductive activity and the rRNA sRNA synthesis in Bel- 7402 hepatocellalar carcinoma cells.展开更多
文摘莲(Nelumbo nucifera Gaertn.)幼胚子叶细胞造淀粉体和分离的造粉质体上均呈现强烈的 Feulgen 反应物质。经 DNA 酶处理后,在子叶细胞造淀粉体上呈 Feulgen 负反应。将分离的造粉质体用特异性的 DNA 荧光染料 DAPI 染色,造粉质体显示蓝色的荧光。实验证明,莲幼胚子叶细胞随着发育时期的增长,造粉质体 DNA 的含量逐渐增加,显示出有规律的动态变化过程。在电镜下观察,造粉质体 DNA 区域外无膜结构存在,故具有原核生物的特征。这种质体 DNA 的纤维丝直径大约为25。
文摘The effect of apatite ranoparticles on proliferation potential and biological behaviour of the human hepatocellular carcinoma in vitro were investigated. After the treatment of Bel- 7402 hepatoceUalar carcinoma cells with apatite nanoparticles at a concentration of 5 × 10^-4 mmol/ L for 4days, Feulgen and AgNOR stain were conducted and the .specimens were observed by microscope. The DNA and AgNOR were quantified with image analysis techniques. It was found that there was a signiftcant decrease of the DNA content （ 58.62 ± 6.52 ） in the nanoparticles treated group compared to the control （ 78.21 ± 4.17 ）. It was further found that there was a decrease in the number of AgNOR granules in the nanoparticle treated group （7.41 ± 1.02） compared to the control group （ 9.95 ± 0.28 ）. The experimental results showed thnt apatite nanoparticles could decrease the DNA reproductive activity and the rRNA sRNA synthesis in Bel- 7402 hepatocellalar carcinoma cells.
