Fibroblast growth factor(FGF)7,a member of FGF family,is initially found to be secreted from mesenchymal cells to repair epithelial tissues.However,its functions in the nervous system are largely unknown.The present s...Fibroblast growth factor(FGF)7,a member of FGF family,is initially found to be secreted from mesenchymal cells to repair epithelial tissues.However,its functions in the nervous system are largely unknown.The present study showed that FGF7 was a neuromodulator localized in the large dense-core vesicles(LDCVs)in nociceptive neurons.FGF7 was mainly expressed in small-diameter neurons of the dorsal root ganglion and could be transported to the dorsal spinal cord.Interestingly,FGF7 was mostly stored in LDCVs that did not contain neuropeptide substance P.Electrophysiological recordings in the spinal cord slice showed that buffer-applied FGF7 increased the amplitude of excitatory post-synaptic current evoked by stimulating the sensory afferent fibers.Behavior tests showed that intrathecally applied FGF7 potentiated the formalin-induced acute nociceptive response.Moreover,both acute and inflammatory nociceptive responses were significantly reduced in Fgf7-deficient mice.These results suggest that FGF7 exerts an excitatory modulation of nociceptive afferent transmission.展开更多
目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体...目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体重变化,另外20只小鼠利用1%甲苯胺蓝染色法观察照射小鼠舌组织溃疡情况,HE染色观察舌组织病理学改变,Ki-67免疫组化检测黏膜角化上皮细胞增殖情况。结果与照射对照组相比,rh KGF和WR2721给药组小鼠30d存活率显著提高,体重恢复较快,角化上皮细胞增殖显著,未发生明显的口腔黏膜炎;CBLB502组小鼠的存活率、体重变化及组织病理学指标与照射对照组比较差异无统计学意义(P>0.05)。结论 rh KGF和WR2721对放射性口腔黏膜炎有较好的防治作用,而CBLB502并不能减轻放射性口腔黏膜炎的发生。展开更多
基金This work was supported by the National Natural Science Foundation of China(31130066)the Strategic Priority Research Program(B)of Chinese Academy of Sciences(XDB01020300).
文摘Fibroblast growth factor(FGF)7,a member of FGF family,is initially found to be secreted from mesenchymal cells to repair epithelial tissues.However,its functions in the nervous system are largely unknown.The present study showed that FGF7 was a neuromodulator localized in the large dense-core vesicles(LDCVs)in nociceptive neurons.FGF7 was mainly expressed in small-diameter neurons of the dorsal root ganglion and could be transported to the dorsal spinal cord.Interestingly,FGF7 was mostly stored in LDCVs that did not contain neuropeptide substance P.Electrophysiological recordings in the spinal cord slice showed that buffer-applied FGF7 increased the amplitude of excitatory post-synaptic current evoked by stimulating the sensory afferent fibers.Behavior tests showed that intrathecally applied FGF7 potentiated the formalin-induced acute nociceptive response.Moreover,both acute and inflammatory nociceptive responses were significantly reduced in Fgf7-deficient mice.These results suggest that FGF7 exerts an excitatory modulation of nociceptive afferent transmission.
文摘目的比较r h KG F、CB L B 5 0 2、W R 2 7 2 1对放射性口腔黏膜炎(R O M)的防治作用。方法健康雄性C57BL/6J小鼠随机分为正常对照组、照射对照组、rh KGF组、CBLB502组及WR2721组。其中24只小鼠观察17Gy头颈部照射小鼠的30d存活率及体重变化,另外20只小鼠利用1%甲苯胺蓝染色法观察照射小鼠舌组织溃疡情况,HE染色观察舌组织病理学改变,Ki-67免疫组化检测黏膜角化上皮细胞增殖情况。结果与照射对照组相比,rh KGF和WR2721给药组小鼠30d存活率显著提高,体重恢复较快,角化上皮细胞增殖显著,未发生明显的口腔黏膜炎;CBLB502组小鼠的存活率、体重变化及组织病理学指标与照射对照组比较差异无统计学意义(P>0.05)。结论 rh KGF和WR2721对放射性口腔黏膜炎有较好的防治作用,而CBLB502并不能减轻放射性口腔黏膜炎的发生。
