Background:Chronic wounds pose a significant surgical challenge,often requiring traditional treatments with limited efficacy.This study explores the promising impact of Shixiang plaster,a classic Chinese ointment,on w...Background:Chronic wounds pose a significant surgical challenge,often requiring traditional treatments with limited efficacy.This study explores the promising impact of Shixiang plaster,a classic Chinese ointment,on wound healing.We investigated the cluster of differentiation 31(CD31)expression,serum fibronectin(FN),and vascular endothelial growth factor(VEGF)levels in SPF rats with induced wounds to elucidate the mechanism behind Shixiang plaster’s effectiveness.We investigated the effect and explored the role of Shixiang plaster on the expression of CD31,serum FN,and VEGF in chronic wounds.Methods:The study involved 36 SPF rats divided into model,rb-bFGF,and Shixiang plaster groups.Penicillin was injected into the rats before modelling for 3 days to prevent infection.The skin was excised 2 cm below the horizontal line of the inferior border of the shoulder bone in the middle of the rat column up to the deep fascial layer and inoculated with a certain concentration of Staphylococcus aureus;the wound was covered aseptically for 3 days.The trauma area of the rats was observed at 3,7,and 14 days,respectively.Histopathology was observed using haematoxylin eosin and Masson staining.CD31 expression was detected using immunohistochemistry staining.FN and VEGF expression was detected using serum ELISA.Statistical analyses were carried out by the method of SPSS.Results:Regarding wound morphology,at 3 days,the recovery area of the Shixiang plaster group was larger than that of the other two groups,at 7 days,the wound healing rate of the Shixiang plaster group was significantly higher,and at 14 days,the wounds of the Shixiang plaster group had been mostly healed,with a healing rate of 98.3%.Haematoxylin eosin staining revealed a large amount of granulation tissue at 3 days in the Shixiang plaster group,and the epidermal scales disappeared at 14 days,with thinner epidermal thickness at 1 lesion and a large reduction in inflammatory cell infiltration.Masson staining showed that at 3,7,and 14 days,blue staining was the most abundant and deeper in the Shixiang plaster group,with richer collagen and a compact tissue matrix.Immunohistochemical testing showed strong positive expression of CD31 in the Shixiang plaster group,with abundant neovascularisation and large official lumens extending towards the surface of the wound.Statistically significant elevated expression of FN at 7 and 14 days was determined by ELISA in the Shixiang plaster group,and VEGF expression was significantly increased at 7 days,but expression had been expressed at a low level at 14 days.Conclusion:Shixiang plaster exhibits remarkable efficacy in healing chronic wounds.The proposed mechanism involves FN’s promotion of angiogenesis and cell proliferation,VEGF’s impact on angiogenesis and inflammation,and CD31’s regulatory role in inhibiting inflammation while promoting angiogenesis.展开更多
目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模...目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模型作为研究模型,分为正常组、假UUO组、UUO组、福辛普利治疗组(F组)、苦参碱大剂量治疗组(E组)和苦参碱小剂量治疗组(D组)。运用免疫组化技术半定量分析实验第7、14 d各组的MM P-3、T IM P-1和FN表达水平。结果UUO组和治疗组的MM P-3表达水平第7、14 d均低于正常组和假UUO组(P<0.05),但三个治疗组的表达水平高于UUO组(P<0.05);T IM P-1和FN在正常组和假UUO组中的表达最低,而三个治疗组的表达均低于UUO组(P<0.05);MM P-3、T IM P-1和FN在E组与F组间的表达差异均无统计学意义(P>0.05);在UUO组中,MM P-3与FN、MM P-3与T IM P-1的免疫组化半定量分析均呈负相关(P<0.01)。结论苦参碱可以部分恢复小管间质MM P-3的表达水平,降低T IM P-1和FN的表达水平,从而延缓肾小管间质纤维化的进程。展开更多
目的:观察高糖高脂刺激下人肾小球系膜细胞增殖分化及FN、TGF-β mRNA和蛋白活性表达变化,探究益气解毒活络中药有效成分防治糖尿病肾病的分子机制,讨论正交配伍中最优药物组合。方法:体外培养人肾小球系膜细胞株,传代3~5代后接种于96...目的:观察高糖高脂刺激下人肾小球系膜细胞增殖分化及FN、TGF-β mRNA和蛋白活性表达变化,探究益气解毒活络中药有效成分防治糖尿病肾病的分子机制,讨论正交配伍中最优药物组合。方法:体外培养人肾小球系膜细胞株,传代3~5代后接种于96孔培养板,采用4因素3水平正交设计方案分为正常对照组,高糖高脂模型组、中药1、2、3、4、5、6、7、8、9组,以相应药物对HMCs干预24、48、72h。MTT法检测24、48、72 h各时间点HMCs增殖分化情况;以qRT-PCR法检测48 h FN、TGF-βmRNA表达;以ELISA法检测48 h FN、TGF-β蛋白活性表达。结果与结论:(1)高糖高脂环境刺激作用下HMCs及胞外基质均能够显著增殖,且在48h达到顶峰;(2)益气解毒活络中药有效成分防治DN作用机制与通过下调高糖高脂作用下HMCs TGF-β、FN mRNA及蛋白活性表达,进而抑制高糖高脂刺激HMCs增殖相关;(3)益气解毒活络中药有效成分最佳配伍为黄芪甲苷中剂量、盐酸小檗碱低剂量、水蛭素低剂量、木犀草苷低剂量。展开更多
基金supported by the Guizhou Provincial Traditional Chinese Medicine Administration Traditional Chinese Medicine and Ethnic Medicine Scientific Research Project(Project number:QZYY-2023-013).
文摘Background:Chronic wounds pose a significant surgical challenge,often requiring traditional treatments with limited efficacy.This study explores the promising impact of Shixiang plaster,a classic Chinese ointment,on wound healing.We investigated the cluster of differentiation 31(CD31)expression,serum fibronectin(FN),and vascular endothelial growth factor(VEGF)levels in SPF rats with induced wounds to elucidate the mechanism behind Shixiang plaster’s effectiveness.We investigated the effect and explored the role of Shixiang plaster on the expression of CD31,serum FN,and VEGF in chronic wounds.Methods:The study involved 36 SPF rats divided into model,rb-bFGF,and Shixiang plaster groups.Penicillin was injected into the rats before modelling for 3 days to prevent infection.The skin was excised 2 cm below the horizontal line of the inferior border of the shoulder bone in the middle of the rat column up to the deep fascial layer and inoculated with a certain concentration of Staphylococcus aureus;the wound was covered aseptically for 3 days.The trauma area of the rats was observed at 3,7,and 14 days,respectively.Histopathology was observed using haematoxylin eosin and Masson staining.CD31 expression was detected using immunohistochemistry staining.FN and VEGF expression was detected using serum ELISA.Statistical analyses were carried out by the method of SPSS.Results:Regarding wound morphology,at 3 days,the recovery area of the Shixiang plaster group was larger than that of the other two groups,at 7 days,the wound healing rate of the Shixiang plaster group was significantly higher,and at 14 days,the wounds of the Shixiang plaster group had been mostly healed,with a healing rate of 98.3%.Haematoxylin eosin staining revealed a large amount of granulation tissue at 3 days in the Shixiang plaster group,and the epidermal scales disappeared at 14 days,with thinner epidermal thickness at 1 lesion and a large reduction in inflammatory cell infiltration.Masson staining showed that at 3,7,and 14 days,blue staining was the most abundant and deeper in the Shixiang plaster group,with richer collagen and a compact tissue matrix.Immunohistochemical testing showed strong positive expression of CD31 in the Shixiang plaster group,with abundant neovascularisation and large official lumens extending towards the surface of the wound.Statistically significant elevated expression of FN at 7 and 14 days was determined by ELISA in the Shixiang plaster group,and VEGF expression was significantly increased at 7 days,but expression had been expressed at a low level at 14 days.Conclusion:Shixiang plaster exhibits remarkable efficacy in healing chronic wounds.The proposed mechanism involves FN’s promotion of angiogenesis and cell proliferation,VEGF’s impact on angiogenesis and inflammation,and CD31’s regulatory role in inhibiting inflammation while promoting angiogenesis.
文摘目的观察苦参碱对单侧输尿管梗阻(UUO)大鼠模型肾小管间质基质金属蛋白酶-3(MM P-3)、基质金属蛋白酶组织抑制物-1(T IM P-1)和纤维联结蛋白(FN)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法实验采用UUO肾间质纤维化模型作为研究模型,分为正常组、假UUO组、UUO组、福辛普利治疗组(F组)、苦参碱大剂量治疗组(E组)和苦参碱小剂量治疗组(D组)。运用免疫组化技术半定量分析实验第7、14 d各组的MM P-3、T IM P-1和FN表达水平。结果UUO组和治疗组的MM P-3表达水平第7、14 d均低于正常组和假UUO组(P<0.05),但三个治疗组的表达水平高于UUO组(P<0.05);T IM P-1和FN在正常组和假UUO组中的表达最低,而三个治疗组的表达均低于UUO组(P<0.05);MM P-3、T IM P-1和FN在E组与F组间的表达差异均无统计学意义(P>0.05);在UUO组中,MM P-3与FN、MM P-3与T IM P-1的免疫组化半定量分析均呈负相关(P<0.01)。结论苦参碱可以部分恢复小管间质MM P-3的表达水平,降低T IM P-1和FN的表达水平,从而延缓肾小管间质纤维化的进程。
文摘目的:观察高糖高脂刺激下人肾小球系膜细胞增殖分化及FN、TGF-β mRNA和蛋白活性表达变化,探究益气解毒活络中药有效成分防治糖尿病肾病的分子机制,讨论正交配伍中最优药物组合。方法:体外培养人肾小球系膜细胞株,传代3~5代后接种于96孔培养板,采用4因素3水平正交设计方案分为正常对照组,高糖高脂模型组、中药1、2、3、4、5、6、7、8、9组,以相应药物对HMCs干预24、48、72h。MTT法检测24、48、72 h各时间点HMCs增殖分化情况;以qRT-PCR法检测48 h FN、TGF-βmRNA表达;以ELISA法检测48 h FN、TGF-β蛋白活性表达。结果与结论:(1)高糖高脂环境刺激作用下HMCs及胞外基质均能够显著增殖,且在48h达到顶峰;(2)益气解毒活络中药有效成分防治DN作用机制与通过下调高糖高脂作用下HMCs TGF-β、FN mRNA及蛋白活性表达,进而抑制高糖高脂刺激HMCs增殖相关;(3)益气解毒活络中药有效成分最佳配伍为黄芪甲苷中剂量、盐酸小檗碱低剂量、水蛭素低剂量、木犀草苷低剂量。
