IN those days, when heavy snow sealed the mountain passes, it was only the sight of red paper-cuts on the windows which said to me there were people living in those houses covered with deep and solid snow. Every time ...IN those days, when heavy snow sealed the mountain passes, it was only the sight of red paper-cuts on the windows which said to me there were people living in those houses covered with deep and solid snow. Every time I travel to the Northwest, I have come across paper-cutters who touch me so deeply that I can’t forget their faces even long after I return to the city. Sometimes in the harsh winters of the Northwest, there is no color but the monotonous white of the snow until the lively red of the paper-cuts spring into sight. These colorful, waiting things like cheerful signals of warmth and humanity,展开更多
【目的】建立高效的新疆早熟陆地棉田间遗传转化与室内潮霉素相结合的筛选体系。【方法】以MS为基本培养基,以去皮早熟陆地棉新陆早17号种子为材料,进行头孢霉素敏感性实验,研究其对棉花植株的影响和抑菌情况;通过在MS培养基上添加不同...【目的】建立高效的新疆早熟陆地棉田间遗传转化与室内潮霉素相结合的筛选体系。【方法】以MS为基本培养基,以去皮早熟陆地棉新陆早17号种子为材料,进行头孢霉素敏感性实验,研究其对棉花植株的影响和抑菌情况;通过在MS培养基上添加不同浓度的潮霉素和IBA,研究新陆早17号最适抗性筛选浓度及最适生根条件。【结果】新陆早17号最适的头孢霉素筛选浓度为500μg/m L,在该浓度下,植株生长不受影响且其鲜重较其他处理增重明显;其对潮霉素反应较为敏感,10μg/m L潮霉素即可抑制棉花根部生长,棉花植株生长矮小叶片发黄;其最适生根培养基为MS＋1.0μg/m L IBA,在该浓度条件下生根率为100%,平均根数8.5根;具有3~4片真叶的生根无菌苗经练苗和移栽后成活率为100%。【结论】为新疆早熟陆地棉田间进行＂喷花法＂的农杆菌遗传转化方式和室内潮霉素抗性筛选鉴定提供了理论基础。展开更多
文摘IN those days, when heavy snow sealed the mountain passes, it was only the sight of red paper-cuts on the windows which said to me there were people living in those houses covered with deep and solid snow. Every time I travel to the Northwest, I have come across paper-cutters who touch me so deeply that I can’t forget their faces even long after I return to the city. Sometimes in the harsh winters of the Northwest, there is no color but the monotonous white of the snow until the lively red of the paper-cuts spring into sight. These colorful, waiting things like cheerful signals of warmth and humanity,
文摘【目的】建立高效的新疆早熟陆地棉田间遗传转化与室内潮霉素相结合的筛选体系。【方法】以MS为基本培养基,以去皮早熟陆地棉新陆早17号种子为材料,进行头孢霉素敏感性实验,研究其对棉花植株的影响和抑菌情况;通过在MS培养基上添加不同浓度的潮霉素和IBA,研究新陆早17号最适抗性筛选浓度及最适生根条件。【结果】新陆早17号最适的头孢霉素筛选浓度为500μg/m L,在该浓度下,植株生长不受影响且其鲜重较其他处理增重明显;其对潮霉素反应较为敏感,10μg/m L潮霉素即可抑制棉花根部生长,棉花植株生长矮小叶片发黄;其最适生根培养基为MS＋1.0μg/m L IBA,在该浓度条件下生根率为100%,平均根数8.5根;具有3~4片真叶的生根无菌苗经练苗和移栽后成活率为100%。【结论】为新疆早熟陆地棉田间进行＂喷花法＂的农杆菌遗传转化方式和室内潮霉素抗性筛选鉴定提供了理论基础。
