Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories h...Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.展开更多
The clinical symptoms of a disorder which is known locally as ＂wool-eating ailment＂ in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emacia...The clinical symptoms of a disorder which is known locally as ＂wool-eating ailment＂ in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emaciation, and wool-eating. The mineral composition of forage, and samples of blood, liver and wool from Guizhou semi-fine wool sheep in affected ranches were compared with those from the unaffected areas. The mean concentration of iron in forage from affected pasture was significantly higher than that from the unaffected pasture. The mean concentration of sulfur in forage from the affected and unaffected pasture was similar and within normal ranges. The mean concentration of sulfur in blood, liver and wool from the affected Guizhou semi-fine wool sheep was significantly lower than that from the unaffected sheep while the content of iron in blood, liver and wool from the affected sheep was significantly higher than that from the unaffected sheep. Oral administration of calcium sulfate prevented and cured the disease. We concluded that the disease of Guizhou semi-fine wool sheep in the Weining County is due to S deficiency caused by the high Fe in forages.展开更多
Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weigh...Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weight（BW）, Weaning Litter Weight （WLW）, Weaning Litter Staple Fineness（WLSF）, One-age Staple Fineness（OASF）, One-age Staple Length （ OASL）, One-age Wool Yield（OAWY）, Main Economic Characters of One-age Aohan fine-wool sheep, Two-age Staple Fineness （TASF）, Two-age Staple Length （TASL）, Two-age Wool Yield （TAWY）, Two-age Weight （TAW） after cropping. [ Result] The heritability was 0.1 -0.7. [ Conclusion~ The heritability of Aohan fine-wool sheep＇s main characters was middle-and-high.展开更多
[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and gr...[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and groin skin （no wool growing） of Aohan fine wool sheep. [Results] 46 immune genes （fold change 〉2.0） were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation.展开更多
The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array...The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain （Xinjiang types） （CMS） and 5 loci in Gansu alpine superfine-wool sheep strain （GSS） groups were found deviated from Hardy-Weinberg equilibrium （HWE）. Genetic drift FsT=0.019 （P〈0.001） and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep （LS） and Hu sheep （HY） reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.展开更多
[Objective]To investigate the population genetic structure of Keerqin fine wool sheep. [Method]The genetic parameters of wool traits in Keerqin fine wool sheep were calculated by employing paternal half sib correlatio...[Objective]To investigate the population genetic structure of Keerqin fine wool sheep. [Method]The genetic parameters of wool traits in Keerqin fine wool sheep were calculated by employing paternal half sib correlation method. [Results]The traits with high heritability( h≥0. 3) included wool length( 1-year old),wool yield( 1-year old) and wool yield( 2-year old); that with medium heritability( 0. 1≤h≤0. 3) included wool fineness( 1-year old),wool fineness( 2-year old),wool length( 3-year old) and wool yield( 3-year old). There were extremely significant positive correlations between wool fineness( 1-year old) and wool fineness( 2-year old),between wool fineness( 1-year old) and wool fineness( 3-year old),between wool length( 1-year old) and wool length( 2-year old),between wool length( 1-year old) and wool length( 3-year old),and between wool fineness( 2-year old) and wool fineness( 3-year old),with the correlation coefficients of 0. 653 0,0. 625 8,0. 489 6,0. 578 8,0. 578 4 and 0. 554 6 respectively. The results showed that the phenotypic correlation was poorer than genetic correlation among the wool traits. [Conclusion]The wool fineness,wool yield and body weight of Keerqin fine wool sheep all fail to meet the excellent level and need to be improved further.展开更多
To investigate the population genetic structure of Keerqin fine wool sheep, the paternal half sib correlation method was employed to estimate the body weight traits, genetic and phenotypic correlation between fleece t...To investigate the population genetic structure of Keerqin fine wool sheep, the paternal half sib correlation method was employed to estimate the body weight traits, genetic and phenotypic correlation between fleece traits in the Keerqin fine wool sheep. The results showed that the correlation coefficients were 0.690 1, 0.593 1, 0.581 2, 0.210 2 for birth weight with weaning weight, body weight at one week of age, body weight at two week of age, and body weight at three week of age, respectively. The genetic correlations were highly significant （ P 〈0.01 ） for the four pairs of traits. The correlation coefficients were 0.373 3, 0.274 1, 0.268 2 for weaning weight with body weight at one week of age, body weight at two week of age, and body weight at three week of age, respectively. The genetic correlations were also extremely significant （ P 〈 0.01 ） for the three pairs of traits. The correlation coefficients were 0.712 0, 0.805 0, 0.717 1,0.701 9, 0.531 6, 0.719 0, 0.681 3 and 0.521 3 for wool fineness at one week and two week of age, wool fineness at one week and three week of age, staple length and fleece weight at one week of age, staple length at one week and three week of age, fleece weight at one week of age and wool fineness at three week of age, wool fineness at two week and three week of age, wool fineness at two week of age and fleece weight at three week of age, and fleece weight at two week and three week of age, respectively. The high genetic correlations were extremely significant （ P 〈0. O1 ） for the eight pairs of traits. The phenotypic correlations were generally lower than the genetic correlations.展开更多
By analyzing the gap between China and foreign countries in fine wool production, combined with the practical experience of fine woolproduction, the application effects of sheep clothing full coverage technology, mech...By analyzing the gap between China and foreign countries in fine wool production, combined with the practical experience of fine woolproduction, the application effects of sheep clothing full coverage technology, mechanical shearing technology, wool grading and collection technology, scale packaging technology and standardized shearing environment control technique were evaluated, in order to provide reference for popularization and application of fine wool production.展开更多
旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸...旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸直率),并对表型数据进行描述性统计和相关性分析。利用绵羊40K液相SNP芯片对全部个体进行基因分型。使用Plink 1.07软件对芯片数据进行质控,使用GCTA软件和PopLDdecay软件对质控数据进行群体结构分析。利用GMEMA混合线性模型对4种羊毛性状进行了全基因组关联分析(genomewide association study,GWAS),利用在线软件对候选基因进行GO和KEGG富集分析。质控后得到329只个体的30079个SNPs位点用于后续分析。通过GWAS分析筛选出4个在全基因组上显著相关的SNPs位点可能影响羊毛经济性状,分别位于1号、6号及8号染色体上。筛选出9个在染色体水平上显著相关的SNPs位点可能对羊毛性状具有潜在意义,分别位于3、5、8、11、18、21、22、25号染色体,寻找到39个可能影响羊毛性状的候选基因。本研究结果为后续探究敖汉细毛羊羊毛性状的遗传机制及分子育种标记开发提供重要参考。展开更多
基金financially supported by projects 200903060CARS-39 from China Agricultural Ministry
文摘Background： Increasing the polyunsaturated fatty acid （PUFA） content and decreasing the saturated fatty acid （SFA） content of mutton can help to improve its nutritional value for consumers. Several laboratories have evaluated the effects of vitamin E on the fatty acid （FA） composition of muscle in sheep. However, little information is available on wool sheep, even though wool sheep breeds are an important source of mutton, especially in northern China where sheep are extensively farmed. The present study was designed to address the effects of vitamin E on muscle FA composition in male Aohan fine-wool sheep. Methods： Forty-two male Aohan fine-wool lambs （5 mo old） with similar initial body weight were randomly divided into seven groups and fed diets supplemented with 0 （control group）, 20, 100, 200, 1,000, 2,000, or 2,400 IU/sheep/d vitamin E for 12 mo. Three lambs from each group were slaughtered to measure vitamin E and FA content in the Iongissimus lumborum （LL） and gluteus medius （GM） muscles. Results： Vitamin E concentrations in the LL and GM increased significantly after 12 mo of vitamin E supplementation （P 〈 0.05）. However, this increase did not occur in a dose-dependent manner because the muscle vitamin E concentration was highest in the 200 IU/sheep/d group. Dietary vitamin E supplementation also caused a significant reduction in SFA content and an increase in monounsaturated FA （MUFA） content in the LL and GM （P 〈 0.05）. All six doses of vitamin E significantly increased cis9 tronsl -conjugated linoleic acid （cgtl -CLA） content in the LL compared with the control group （P 〈 0.05）. Conclusions： Dietary supplementation with vitamin E increased muscle vitamin E content and improved the nutritional value of mutton by decreasing SFA content and increasing MUFA and c9tl 1-CLA contents in Aohan fine-wool sheep. These effects were greatest in sheep fed a diet containing 200 IU/sheep/d vitamin E.
基金supported financially by the National Natural Science Foundation of China (40930533/ D011004)the China Agriculture Research System (CARS-40-30)+2 种基金the Science Foundation of Bijie District Grant, China (200905)the Guizhou Provincial Key Technologies R&D Program, China (NY[2010]3041)the Guizhou Governor Foundation, China (2009129)
文摘The clinical symptoms of a disorder which is known locally as ＂wool-eating ailment＂ in Guizhou semi-fine wool sheep in the Weining County of Guizhou Province, China, are poor weight gain, lost appetite, pica, emaciation, and wool-eating. The mineral composition of forage, and samples of blood, liver and wool from Guizhou semi-fine wool sheep in affected ranches were compared with those from the unaffected areas. The mean concentration of iron in forage from affected pasture was significantly higher than that from the unaffected pasture. The mean concentration of sulfur in forage from the affected and unaffected pasture was similar and within normal ranges. The mean concentration of sulfur in blood, liver and wool from the affected Guizhou semi-fine wool sheep was significantly lower than that from the unaffected sheep while the content of iron in blood, liver and wool from the affected sheep was significantly higher than that from the unaffected sheep. Oral administration of calcium sulfate prevented and cured the disease. We concluded that the disease of Guizhou semi-fine wool sheep in the Weining County is due to S deficiency caused by the high Fe in forages.
基金supported by Key Projected Supported by Ministry of National Science and Technology and Agricultural Science and Technology Achievement Reformer Fund projects (04EFN211500036)
文摘Objective] This study aimed to estimate the heritability of Aohan fine-wool sheep＇s main economic characters. [ Method] Half-sib correlation in sire method was adopted to estimate Aohan file-wool sheep＇s Birth Weight（BW）, Weaning Litter Weight （WLW）, Weaning Litter Staple Fineness（WLSF）, One-age Staple Fineness（OASF）, One-age Staple Length （ OASL）, One-age Wool Yield（OAWY）, Main Economic Characters of One-age Aohan fine-wool sheep, Two-age Staple Fineness （TASF）, Two-age Staple Length （TASL）, Two-age Wool Yield （TAWY）, Two-age Weight （TAW） after cropping. [ Result] The heritability was 0.1 -0.7. [ Conclusion~ The heritability of Aohan fine-wool sheep＇s main characters was middle-and-high.
基金Supported by Project of National Hair Sheep Industry Technology System(CARS-40)~~
文摘[Objective] To get major genes for wool traits regulation from immune genes. [Methods] Microarray technology was used to detect differentially expressed immune genes between body side skin （more wool growing） and groin skin （no wool growing） of Aohan fine wool sheep. [Results] 46 immune genes （fold change 〉2.0） were identified and classified, and then 6 of which were selected for QPCR confir- mation. The degree of consistency of the QPCR and microarray results was 66.67%, [Conclusion] Immune privilege may participate in wool growth regulation.
基金sponsored by the Earmarked Fund for Modern China Wool & Cashmere Technology Research System (CARS-40-03)the National Natural Science Foundation for Young Scholars of China (31402057)Project support was provided by the ASTIP (Agricultural Science and Technology Innovation Program) for Genetic Resource and Breeding of Fine-Wool Sheep, Chinese Academy of Agricultural Sciences
文摘The aim of our present study was to construct genetic structure and relationships among Chinese fine-wool sheep breeds. 46 individuals from 25 breeds or strains were genotyped based on the Illumina Ovine 50K SNP array. Meanwhile, genetic variations among 482 individuals from 9 populations were genotyped with 10 microsatellites. In this study, we found high genetic polymorphisms for the microsatellites, while 7 loci in the Chinese superfine Merino strain （Xinjiang types） （CMS） and 5 loci in Gansu alpine superfine-wool sheep strain （GSS） groups were found deviated from Hardy-Weinberg equilibrium （HWE）. Genetic drift FsT=0.019 （P〈0.001） and high gene flows were detected in all the 7 fine-wool sheep populations. Phylogenetic analysis showed fine-wool sheep populations were clustered in a group independent from the Chinese indigenous breeds such that the 7 fine-wool sheep clustered distinct from Liangshan semifine-wool sheep （LS） and Hu sheep （HY） reflected by different population differentiation analyses. Overall, our findings suggested that all fine-wool sheep populations have close genetic relationship, which is consistent with their breeding progress. These populations, therefore, can be regarded as open-breeding populations with high levels of gene flows. Furthermore, the two superfine-wool strains, viz., CMS and GSS, might be formed by strong artificial selection and with frequent introduction of Australian Merino. Our results can assist in breeding of superfine-wool sheep and provide guidance for the cultivation of new fine-wool sheep breeds with different breeding objectives.
基金Supported by Scientific Research Fund of Inner Mongolia University for the Nationalities(NMD1339)
文摘[Objective]To investigate the population genetic structure of Keerqin fine wool sheep. [Method]The genetic parameters of wool traits in Keerqin fine wool sheep were calculated by employing paternal half sib correlation method. [Results]The traits with high heritability( h≥0. 3) included wool length( 1-year old),wool yield( 1-year old) and wool yield( 2-year old); that with medium heritability( 0. 1≤h≤0. 3) included wool fineness( 1-year old),wool fineness( 2-year old),wool length( 3-year old) and wool yield( 3-year old). There were extremely significant positive correlations between wool fineness( 1-year old) and wool fineness( 2-year old),between wool fineness( 1-year old) and wool fineness( 3-year old),between wool length( 1-year old) and wool length( 2-year old),between wool length( 1-year old) and wool length( 3-year old),and between wool fineness( 2-year old) and wool fineness( 3-year old),with the correlation coefficients of 0. 653 0,0. 625 8,0. 489 6,0. 578 8,0. 578 4 and 0. 554 6 respectively. The results showed that the phenotypic correlation was poorer than genetic correlation among the wool traits. [Conclusion]The wool fineness,wool yield and body weight of Keerqin fine wool sheep all fail to meet the excellent level and need to be improved further.
基金Supported by Scientific Research Fund of Inner Mongolia University for the Nationalities(NMD1339)Natural Science Foundation of Inner Mongolia Autonomous Region(20080404MS0401)
文摘To investigate the population genetic structure of Keerqin fine wool sheep, the paternal half sib correlation method was employed to estimate the body weight traits, genetic and phenotypic correlation between fleece traits in the Keerqin fine wool sheep. The results showed that the correlation coefficients were 0.690 1, 0.593 1, 0.581 2, 0.210 2 for birth weight with weaning weight, body weight at one week of age, body weight at two week of age, and body weight at three week of age, respectively. The genetic correlations were highly significant （ P 〈0.01 ） for the four pairs of traits. The correlation coefficients were 0.373 3, 0.274 1, 0.268 2 for weaning weight with body weight at one week of age, body weight at two week of age, and body weight at three week of age, respectively. The genetic correlations were also extremely significant （ P 〈 0.01 ） for the three pairs of traits. The correlation coefficients were 0.712 0, 0.805 0, 0.717 1,0.701 9, 0.531 6, 0.719 0, 0.681 3 and 0.521 3 for wool fineness at one week and two week of age, wool fineness at one week and three week of age, staple length and fleece weight at one week of age, staple length at one week and three week of age, fleece weight at one week of age and wool fineness at three week of age, wool fineness at two week and three week of age, wool fineness at two week of age and fleece weight at three week of age, and fleece weight at two week and three week of age, respectively. The high genetic correlations were extremely significant （ P 〈0. O1 ） for the eight pairs of traits. The phenotypic correlations were generally lower than the genetic correlations.
基金Supported by Special Fund for Agricultural Science and Technology Innovation Project in Gansu Province(GNCX-2014-48)
文摘By analyzing the gap between China and foreign countries in fine wool production, combined with the practical experience of fine woolproduction, the application effects of sheep clothing full coverage technology, mechanical shearing technology, wool grading and collection technology, scale packaging technology and standardized shearing environment control technique were evaluated, in order to provide reference for popularization and application of fine wool production.
文摘旨在利用全基因组关联分析探寻敖汉细毛羊羊毛性状新的分子标记和候选基因。本研究采集1~2周岁的健康敖汉细毛羊耳组织与羊毛作为试验素材,其中,母羊248只,公羊81只,总计329只。羊毛进行性状测定(包括纤维直径、自然长度、伸直长度、伸直率),并对表型数据进行描述性统计和相关性分析。利用绵羊40K液相SNP芯片对全部个体进行基因分型。使用Plink 1.07软件对芯片数据进行质控,使用GCTA软件和PopLDdecay软件对质控数据进行群体结构分析。利用GMEMA混合线性模型对4种羊毛性状进行了全基因组关联分析(genomewide association study,GWAS),利用在线软件对候选基因进行GO和KEGG富集分析。质控后得到329只个体的30079个SNPs位点用于后续分析。通过GWAS分析筛选出4个在全基因组上显著相关的SNPs位点可能影响羊毛经济性状,分别位于1号、6号及8号染色体上。筛选出9个在染色体水平上显著相关的SNPs位点可能对羊毛性状具有潜在意义,分别位于3、5、8、11、18、21、22、25号染色体,寻找到39个可能影响羊毛性状的候选基因。本研究结果为后续探究敖汉细毛羊羊毛性状的遗传机制及分子育种标记开发提供重要参考。
