Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a~1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of ClCHYB(annotated asβ-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and ClCHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

Zinc finger protein 296 promotes hepatocellular carcinoma progression via intervening interaction between macrophages and B cells

Objective:Hepatocellular carcinoma(HCC)is a prevalent malignancy with poor survival.Different cell types in the tumor microenvironment participate in the tumorigenesis and progression of HCC.This study aimed to analyze the immune microenvironment of HCC and its relationship with clinical outcomes.Methods:We analyzed HCC RNA-seq for cell type identification and prognosis by estimating relative subsets of RNA transcripts using CIBERSORTx.The interaction between B cells and macrophages in HCC was analyzed using a Hepa1-6 orthotopic transplantation mouse model and flow cytometry.The effect of Zinc finger protein 296(ZNF296)on the interaction of B cells and macrophages was verified using human HCC tissues analyzed through western blot,quantitative real-time polymerase chain reaction(qPCR),and multiplex immunofluorescence.A comparative analysis of immune cells associated with HCC prognosis was performed using RNA-seq data from The Cancer Genome Atlas(TCGA),bulk multimodal data,and single-cell transcriptomic data from existing HCC single-cell transcriptomic data employing the Single Cell Inferred Site Specific Omics Resource for Tumor Microenvironments(SCISSOR).Results:Liver hepatocellular carcinoma(LIHC)RNA-seq analysis of TCGA showed that high eosinophil infiltration promoted HCC progression.The proportion of B cells correlated with that of macrophages(r=−0.24)and affected the infiltration and programmed death ligand 1(PD-L1)expression of macrophages in HCC.ZNF296 may participate in the interaction between B cells and macrophages to accelerate the HCC progression by regulating PAFAH1B3 and H2AFX.Moreover,ZNF296 expression positively correlated with LAG3(r=0.27)and CTLA4(r=0.31)expression levels.Among the immune cell phenotypes related to survival and death identified by SCISSOR analysis,T cells correlated with an excellent prognosis of HCC.The normal function of liver and dendritic cells was also associated with a good prognosis in HCC.Conclusions:This study analyzed the interaction of the immune microenvironment with HCC prognosis,identifying ZNF296 as a promising diagnostic and therapeutic target for HCC.

Zinc finger 4 negatively controls the transcriptional activator Fzf1 in Saccharomyces cerevisiae

Fzf1 is a Saccharomyces cerevisiae transcription factor containing five zinc fingers(ZFs).It regulates the expression of at least five downstream genes,including SSU1,YHB1,DDI2/3,and YNR064c,by recognizing a consensus sequence,CS2,found in these gene promoters.These gene products are involved in cellular responses to various chemical stresses.For example,SsU1 encodes a sodium sulfite efflux protein that confers sulfite resistance.However,the underlying molecular mecha-nism through which Fzf1 responds to chemical stress and coordinates target gene activation remains elusive.Interestingly,several mutations in the fourth ZF(ZF4)of Fzf1 have previously been reported to confer either sulfite resistance or elevated basal-level expression of YHB1,indicating that ZF4 negatively impacts Fzf1 activity.Since ZF4 is dispensable for CS2 binding in vitro,we hypothesized that ZF4 is a negative regulator of Fzf1 and that chemically induced Fzf1-regulated gene expression occurs via de-repression.All five genes examined were cross-induced by corresponding chemicals in an Fzf1-dependent manner,and all three ZF4 mutations and a ZF4 deletion conferred increased basal-level expression and ssU1-dependent sulfite resistance.A ZF4 deletion did not alter the target DNA binding,consistent with the observed codominant phenotype.These observations collectively reveal that Fzf1 remains inactive by default at the target promoters and that its activation is at least partially achieved by self-derepression through chemical modification and/or a conformational change.

Analysis of the Effect of Intensive Nursing Care on Improving Sleep Quality During the Perioperative Period of Severed Finger Reimplantation

Objective:To investigate the clinical effect of perioperative intensive nursing on patients who underwent severed finger reimplantation and its influence on sleep quality.Methods:62 patients who underwent severed finger reimplantation between January 2023 and December 2023 were included.They were divided into two groups:the observation group(n=31)and the control group(n=31).The control group received basic nursing interventions,while the observation group received intensive nursing care.The comparison parameters included visual analog pain score(VAS),incidence of vascular crises,length of hospitalization,Pittsburgh Sleep Quality Index(PSQI),Hamilton Depression Score(HAM-D),Hamilton Anxiety Score(HAM-A),and patient satisfaction.Results:Postoperative VAS score,incidence of vascular crisis,hospitalization time,PSQI score,and HAM-A and HAM-D scores of the observation group were lower than those of the control group(P<0.05).Meanwhile,the patient satisfaction of the observation group was higher than that of the control group(P<0.05).Conclusion:Perioperative intensive nursing care for patients undergoing severed finger reimplantation demonstrates significant benefits.It reduces postoperative pain,lowers the incidence of vascular crises,shortens hospitalization durations,enhances sleep quality,alleviates negative emotions,and improves nursing satisfaction.These findings underscore the importance and applicability of such care practices.

水稻与拟南芥PHDfinger蛋白的系统分析

PHD finger (planthomeodomainfinger)是具Cys4 His Cys3 特征结构的锌指蛋白结构域 ,它广泛存在于植物或动物转录调节蛋白中。此类蛋白在植物或动物蛋白质组中具有多个功能各异的家族成员 ,并在发育中扮演重要的角色。鉴定了水稻 4 4个推定的非冗余PHD finger蛋白 ,并与拟南芥蛋白质组中 4 5个该家族成员进行了比较 ;所构建的 2棵进化树涵盖了这 89个蛋白质 ,暗示水稻、拟南芥与人的PHD

沙田柚RING-H2 finger基因的生物信息学分析

为探讨沙田柚自交不亲和性的机理,利用高通量测序技术对沙田柚自交和异交花柱进行转录组测序,通过差异分析得到了沙田柚RING-H2 finger基因序列。采用生物信息学方法,对其编码的蛋白质从序列特征、理化性质、跨膜结构域、高级结构以及功能域等方面进行了预测和分析。结果表明:该基因全长为845 bp,开放阅读框(ORF)全长为387 bp,编码128个氨基酸,编码蛋白质的分子量为13.61 KDa,理论等电点为4.26;该蛋白质含有一个植物RING-H2 finger蛋白家族的保守结构域,为疏水性不稳定蛋白。氨基酸序列分析表明,其编码的氨基酸与甜橙(Cs4g15340.1)RING-H2 finger蛋白的相似度为98%。这些分析结果可为今后深入研究该蛋白的结构特征和功能提供参考。

Effects of Plant Spacing and Finger Sticking Area Settings for Machine Transplanting on Yield of Double-season Early Rice

The effects of rice transplanters＇ transplanting settings（plant spacing and finger sticking area） on rice yield were investigated in two early-season rice cultivars：hybrid rice cultivar（Zhuliangyou 819） and conventional rice cultivar（Zhongjiazao 17）.The results showed that,different transplanting settings resulted in different numbers of transplanted basic seedlings,and the rice yield was significantly correlated with the number of transplanted basic seedlings.The number of transplanted basic seedlings increased with the decrease of plant spacing and the increase of finger sticking area.The rate of lost hills of machine transplanting decreased with the increase of finger sticking area.The leaf area index and amount of dry matter accumulation were highly correlated with the number of transplanted basic seedlings.The average yield of the rice transplanted with a plant spacing of11.5 cm was higher than that with a plant spacing of 13.8 cm.In the field transplanted with the rice under the plant spacing of 13.8 cm,the rice yield increased with the increase of finger sticking area,and the finger sticking area of 2.23 cm2 showed the highest rice yield of 8 174.16 kg/hm2 of Zhuliangyou 819 and 7 925.54kg/hm2 of Zhongjiaozao17.In the field transplanted with the rice under the plant spacing of 11.5 cm,the rice yield was the highest with the finger sticking area of 1.83 cm2,and then decreased with the large finger sticking area.Under the plant spacing of 11.5 cm,the highest rice yields of Zhuliangyou 819 and Zhongjiaozao17 were 8 492.94 kg/hm2 and 8 000.23 kg/hm2,respectively.Therefore,the preferred machine transplanting settings for double-season early rice are a plant spacing of11.5 cm and a finger sticking area of 1.83 cm2,while the finger sticking area should be increased to 2.23 cm2 for conventional rice cultivars.

Therapeutic targeting of cellular prion protein: toward the development of dual mechanism anti-prion compounds

PrPSc,a misfolded,aggregation-prone isoform of the cellular prion protein(PrPC),is the infectious prion agent responsible for fatal neurodegenerative diseases of humans and other mammals.PrPSccan adopt different pathogenic conformations(prion strains),which can be resistant to potential drugs,or acquire drug resistance,posing challenges for the development of effective therapies.Since PrPCis the obligate precursor of any prion strain and serves as the mediator of prion neurotoxicity,it represents an attractive therapeutic target fo r prion diseases.In this minireview,we briefly outline the approaches to target PrPCand discuss our recent identification of Zn(Ⅱ)-Bn PyP,a PrPC-targeting porphyrin with an unprecedented bimodal mechanism of action.We argue that in-depth understanding of the molecular mechanism by which Zn(Ⅱ)-Bn PyP targets PrPCmay lead toward the development of a new class of dual mechanism anti-prion compounds.

植物非生物逆境胁迫相关RING finger蛋白

RING finger蛋白是锌指蛋白类中一个庞大的蛋白家族,已成为拟南芥第三大蛋白家族,在水稻中也发现了488个。最典型的结构特点是序列内包含环指结构域(RING finger domain)。RING finger蛋白主要通过泛素化途径参与到植物细胞的生理生化过程。介绍了植物RING finger蛋白的结构特点和分类,综述它们的亚细胞定位,重点阐述它们在响应干旱胁迫、温度胁迫和盐胁迫等非生物逆境胁迫的调控作用。

绵羊痘病毒甘肃古浪株RING finger基因的克隆表达及序列分析

【目的】对绵羊痘病毒甘肃古浪株RING finger蛋白的基因进行克隆,表达以及序列分析,初步探究绵羊痘病毒RING finger蛋白是否具有E3泛素连接酶活性,为阐明其在绵羊痘病毒感染过程中对泛素蛋白酶体系统的调控作用奠定基础.【方法】以绵羊痘病毒甘肃古浪株DNA为模板,通过PCR扩增 RING finger 基因.利用Pfam数据库、DNAstar等软件进行序列及遗传进化分析;将 RING finger 基因片段插入原核表达载体pGEX-4T-1中,构建pGEX-SPPVRFP重组表达载体,在大肠杆菌BL21(DE3)中诱导表达,并进行SDS-PAGA和Western-blot分析.【结果】绵羊痘病毒 RING finger 基因由723个核苷酸组成,编码的240个氨基酸的分子量约为28.5 ku,具有RING finger结构域.不同羊痘病毒株间 RING finger 基因核苷酸序列同源性高达99.2%,氨基酸序列同源性高达98.3%.不同的RING finger蛋白都含有8个保守的半胱氨酸和组氨酸.SDS-PAGA分析显示,重组SPPVRFP大小约为55 ku,Western-blot分析显示,重组SPPVRFP不能与绵羊痘病毒阳性血清反应.【结论】成功克隆、表达并纯化了绵羊痘病毒 RING finger 基因,对SPPV GS-GL株RING-finger蛋白进行序列分析,推测其可能具有E3泛素连接酶活性.

基于虚拟力弹簧方法的Partial Finger动力学建模与仿真

为了掌握Partial Finger的运动学规律和动力学特性,判断其结构参数对Partial Finger使用性能的影响,准确评价其真实的工作状态,根据Partial Finger的机械结构,建立了基于虚拟力弹簧方法的Partial Finger动力学模型,并利用Matlab软件对所建立的动力学模型进行数值求解,通过对比相同工作状态下的动力学模型以及Adams仿真结果,初步确定虚拟力弹簧的弹性系数量级取104N/mm时,动力学模型和Adams仿真所得结果一致,证明动力学模型正确,并为Partial Finger机械结构的进一步设计、完善提供了理论依据。

keep one's fingers crossed

keep one s fingers crossed 的字面意思是将手指交叉起来,通常是用中指压住食指,也有人习惯于食指压住中指。这个短语的比喻意义是 to hope that nothing will happento upset one s plans,即“希望一切如愿”“但愿别出差错”。如:He is keeping his fingers crossed that itwon t rain on Sunday when he wants to playfootball.他想星期天去踢球。

SARS冠状病毒蛋白酶3CL^(pro) N-finger多肽是酶活性所必需(英文)

构建了 SARS冠状病毒主要蛋白酶3CLpro 及其缺失N 端七肽即N-finger的突变体3CLpro(?1–7)融合表达质粒,并于大肠杆菌表达系统中表达。得到的融合蛋白经肠激酶酶切,亲和层析,最终得到纯化的3CLpro及其突变体 3CLpro(?1–7)。酶活性实验显示,去除 N-finger多肽的突变体3CLpro(?1–7)丧失了3CLpro所具有的对含有其自动水解位点的荧光底物的水解活性,表明处于非酶活性中心的N-finger 多肽是酶活性所必需的。利用固相合成法,进一步合成了N-finger七肽。酶抑制实验表明N-finger七肽表现了对3CLpro 部分竞争抑制活性。

残疾人用Partial Finger机构研究

为了解决手指基指节截断患者无法安装功能型假肢的问题,研制了残疾人用Partial Finger.先分析了手指基指节截断患者的手部结构特点,基于被动驱动原理设计了Partial Finger的机械本体.基于拟人化的思想研究了手指的关节运动规律,设计以近似等比例传动的杆件参数.进行了运动学分析及样机性能实验.Partial Finger样机外形比成年人小指略小,采用残存基指节驱动,具有侧摆和张合两个自由度,手指关节以近似定传动比传动.实验结果表明:Partial Finger可单手安装和拆卸,指尖最大出力10.7N,外形和运动状态与人手指相似,疲劳实验5万次以上未出现问题,配合其他手指可完成近95%的手部功能.

植物逆境相关RING finger蛋白的研究进展

锌指蛋白是植物转录因子中的一个大家族,RING finger蛋白属于其中一个分支,具有环状结构域的典型特征结构,在植物生长发育以及响应逆境胁迫等方面起着重要作用。主要综述了RING finger蛋白的结构特点、分类和亚细胞定位,重点概括了RING finger蛋白在参与非生物与生物胁迫耐受性等方面的研究成果,并对其进一步的深入研究进行了展望,为将来利用植物RING finger蛋白基因创造抗逆新种质提供参考。

Comparative clinical study of ultrasound-guided A1 pulley release vs open surgical intervention in the treatment of trigger finger

AIM To investigate the effectiveness of ultrasound-guided release of the first annular pulley and compare results with the conventional open operative technique.METHODS In this prospective randomized, single-center, clinical study, 32 patients with trigger finger or trigger thumb, grade Ⅱ-Ⅳ according to Green classification system, were recruited. Two groups were formed; Group A(16 patients) was treated with an ultrasound-guided percutaneous release of the affected A1 pulley under local anesthesia. Group B(16 patients) underwent an open surgical release of the A1 pul ey, through a 10-15 mm incision. Patients were assessed pre- and postoperatively(follow-up:2,4 and 12 wk) by physicians blinded to the procedures. Treatment of triggering(primary variable of interest) was expressed as the "success rate" per digit. The time for taking postoperative pain killers, range of motion recovery, QuickD ASH test scores(Greek version), return to normal activities(including work), complications and cosmetic results were assessed.RESULTS The success rate in group A was 93.75%(15/16) and in group B 100%(16/16). Mean times in group A patients were 3.5 d for taking pain killers, 4.1 d for returning to normal activities, and 7.2 and 3.9 d for complete extension and flexion recovery, respectively. Mean Quick DASH scores in group A were 45.5 preoperatively and, 7.5, 0.5 and 0 after 2, 4, and 12 wk postoperatively. Mean times in group B patients were 2.9 d for taking pain killers, 17.8 d for returning to normal activities, and 5.6 and 3 d for complete extension and flexion recovery. Mean QuickD ASH scores in group B were 43.2 preoperatively and, 8.2, 1.3 and 0 after 2, 4, and 12 wk postoperatively. The cosmetic results found excellent or good in 87.5%(14/16) of group A patients, while in 56.25%(9/16) of group B patients were evaluated as fair or poor.CONCLUSION Treatment of the trigger finger using ultrasonography resulted in fewer absence of work days, and better cosmetic results, in comparison with the open surgery technique. It is a promising method that represents excellent results without major complications, so that it could be possibly be established as a first-line treatment in the trigger finger's disease.

Kinetostatics Analysis of a Novel 6-DOF Parallel Manipulator with Three Planar Limbs and Equipped with Three Fingers

It is significant to develop a robot hand with high rigidity by a 6-DOF parallel manipulator（PM）.However,the existing6-DOF PMs include spherical joint which has less capability of pulling force bearing,less rotation range and lower precision under alternately heavy loads.A novel 6-DOF PM with three planar limbs and equipped with three fingers is proposed and its kinematics and statics are analyzed systematically.A 3-dimension simulation mechanism of the proposed manipulator is constructed and its structure characteristics is analyzed.The kinematics formulae for solving the displacement,velocity,acceleration of the platform,the active legs and the fingers are established.The statics formulae are derived for solving the active forces of PM and the finger mechanisms.An analytic example is given for solving the kinematics and statics of proposed manipulator and the analytic solved results are verified by the simulation mechanism.It is proved from the error analysis of analytic solutions and simulation solutions that the derived analytic formulae are correct and provide the theoretical and technical foundations for its manufacturing,control and application.

Fingered Intrusion of Shallow Saucer-shaped Igneous Sills: Insights from the Jiaojiang Sag, East China Sea

An extensive suite of igneous sills was intruded into the Tertiary sedimentary section of the Jiaojiang sag, East China Sea. This suite has been well imaged offshore through 2D and 3D seismic surveys, showing a close relationship with CO2 content in nearby gas discoveries. A new observational model, which incorporates simple upward propagation, simple horizontal propagation, and transgressive propagation, was proposed to interpret these sill intrusions. In this model, the source magma of the saucer-shaped sills was injected from their lowest points near the center. The transgressive propagation can be interpreted as a combination of the vertical and horizontal propagation. Most shallow sub-volcanic intrusions in the Jiaojiang sag exhibit fingered characteristics, both vertically and horizontally. The vertical fingered propagation produced saucer-shaped sills arranged in the flower style. Along the brims of lower sills could usually be found the upper sills, which are interpreted to have formed simultaneously with or later than the lower feeder sills. In the second type, the chilled paths of the older sills were reutilized by subsequent intrusions. The horizontal fingered propagation formed sheet intrusions that exhibit groove, lobate, tubular, and crevasse splay- like geometry in plan view. In 3D view, the sheet intrusions have still preserved the lower center and higher rim that similar with the sub-rounded saucer-shaped sills. Although fracture propagation may be important, flow inflation was the key mechanism of magma intrusion. Further consideration of the mechanisms underlying sill formation may help explain the fingered characteristics of sill propagation.

Dynamic Behavior Analysis of Finger Seal Based on Equivalent Dynamic Model

Finger seal is a new technology developed for gas path sealing in gas turbine engine.It has been paid attention to its good sealing performance and lower manufacture cost.The vibration behavior of finger seal is not be considered in performance analysis for the contact finger seal with compliant geometric configuration,then this will influence the reliability of the performance analysis and the parametric design of a finger seal.According to the dynamic character of finger seal,the harmonic vibration and free vibration models of a repetitive section of a two-layer finger seal that contains one high and one low finger seal are established separately.The dynamic behavior of finger seal in different working conditions is obtained based on the dynamic response analysis of finger＇s vibrations.The dynamic low hysteresis and low wear terms based on the equivalent dynamic model of finger seal are put forward,which can be used to predict the dynamic behavior of finger seal.As an example,the dynamic behaviors of an 8-layer finger seal and a 10-layer finger seal were predicted,and the validity of the prediction was demonstrated by the leakage test results of these two finger seals.The work presented here not only could make the dynamic analysis of finger seal with complicated structure simple and easy especially for multi-layer finger seal system,but also suggest a useful method for designing the finger seal with good dynamic behavior by means of different seal layers assembly.

Human RING finger protein ZNF645 is a novel testis-specific E3 ubiquitin ligase

A large number of testis-specific genes are involved in the complex process of mammalian spermatogenesis. Identification of these genes and their roles is important for understanding the mechanisms underlying spermatogenesis. Here we report on a novel human RING finger protein, ZNF645, which contains a C3HC4 RING finger domain, a C2H2 zinc-finger domain, and a proline-rich region, indicating that it has a structure similar to that of the c-Cbl-like protein Hakai. ZNF645 was exclusively expressed in normal human testicular tissue. Immunohistochemical analysis confirmed that ZNF645 protein was present in spermatocytes, round and elongated spermatids, and Leydig cells. Immunofluorescence staining of mature sperms further showed that the ZNF645 protein was localized over the postacrosomal perinuclear theca region and the entire length of sperm tail. An in vitro ubiquitination assay indicated that the RING finger domain of the ZNF645 protein had E3 ubiquitin ligase activity. Therefore, we suggest that ZNF645 might act as an E3 ubiquitin-protein ligase and play a role in human sperm production and quality control.