The losses caused by outbreaks of various diseases in aquaculture intensive farming are becoming more and more serious. Fish diseases have become one of the problems that constrain the healthy development of aquacultu...The losses caused by outbreaks of various diseases in aquaculture intensive farming are becoming more and more serious. Fish diseases have become one of the problems that constrain the healthy development of aquaculture. Among them,fish parasitic diseases have also caused some losses to the aquaculture industry. In this paper,17 species of fish parasites in Huaihua area,belonging to 15 genera in 13 families of 13 orders,are reviewed. In particular,the 10 kinds of fish parasitic diseases that are more harmful to farmed fishes are described in detail in order to provide a basis for the prevention of fish diseases in the future.展开更多
Saprolegniasis,caused by Saprolegnia infection,is one of the most common diseases in freshwater fish.Our study aimed to determine the epidemiological characteristics of saprolegniasis in Chinese regions of high incide...Saprolegniasis,caused by Saprolegnia infection,is one of the most common diseases in freshwater fish.Our study aimed to determine the epidemiological characteristics of saprolegniasis in Chinese regions of high incidence.Saprolegnia were isolated and identified by morphological and molecular methods targeting the internal transcribed spacer(ITS)ribosomal DNA(rDNA)and building neighbor-joining(NJ)and maximum parsimony(MP)phylogenetic trees.The ITS sequences of eight isolated strains were compared with GenBank sequences and all strains fell into three clades:CLADE1(02,LP,04 and 14),CLADE2(S1),and CLADE3(CP,S2,L5 and the reference ATCC200013).Isolates 02 and LP shared 80%sequence similarity with S.diclina,S.longicaulis,S.ferax,S.mixta,and S.anomalies.Further,isolates 04 and 14 shared 80%similarity with S.bulbosa and S.oliviae.Finally,extremely high ITS sequence similarities were identified between isolates S1 and S.australis(100%);CP and S.hypogyna(96%);and S2,L5,ATCC200013 and S.salmonis(98%).This research provides insights into the identification,prevention and control of saprolegniasis pathogens and the potential development of effective drugs.展开更多
文摘The losses caused by outbreaks of various diseases in aquaculture intensive farming are becoming more and more serious. Fish diseases have become one of the problems that constrain the healthy development of aquaculture. Among them,fish parasitic diseases have also caused some losses to the aquaculture industry. In this paper,17 species of fish parasites in Huaihua area,belonging to 15 genera in 13 families of 13 orders,are reviewed. In particular,the 10 kinds of fish parasitic diseases that are more harmful to farmed fishes are described in detail in order to provide a basis for the prevention of fish diseases in the future.
基金This work is financially supported by the National Key Technology R&D Program(Grant No.2013BAD13B03).
文摘Saprolegniasis,caused by Saprolegnia infection,is one of the most common diseases in freshwater fish.Our study aimed to determine the epidemiological characteristics of saprolegniasis in Chinese regions of high incidence.Saprolegnia were isolated and identified by morphological and molecular methods targeting the internal transcribed spacer(ITS)ribosomal DNA(rDNA)and building neighbor-joining(NJ)and maximum parsimony(MP)phylogenetic trees.The ITS sequences of eight isolated strains were compared with GenBank sequences and all strains fell into three clades:CLADE1(02,LP,04 and 14),CLADE2(S1),and CLADE3(CP,S2,L5 and the reference ATCC200013).Isolates 02 and LP shared 80%sequence similarity with S.diclina,S.longicaulis,S.ferax,S.mixta,and S.anomalies.Further,isolates 04 and 14 shared 80%similarity with S.bulbosa and S.oliviae.Finally,extremely high ITS sequence similarities were identified between isolates S1 and S.australis(100%);CP and S.hypogyna(96%);and S2,L5,ATCC200013 and S.salmonis(98%).This research provides insights into the identification,prevention and control of saprolegniasis pathogens and the potential development of effective drugs.
