This study was conducted to determine the contamination data of some heavy metals in 10 fish tissue samples of two different fish species---common carp and Northern pike from four different fish ponds and to compare t...This study was conducted to determine the contamination data of some heavy metals in 10 fish tissue samples of two different fish species---common carp and Northern pike from four different fish ponds and to compare the level of contamination and the differences in the concentration of these elements between these two fish species and between compared fish ponds. In various fish ponds, metal concentrations in the carp muscle tissue varied depending on the metal type. Between compared fish ponds, there were significant differences (P 〈 0.01) in the concentration in muscle carp tissue, except for cadmium. Metal concentrations in the pike muscle tissue also varied among fish ponds, depending on the type of metal. In all compared cases, there were significant differences between metal concentration in pike muscle tissue (P 〈 0.01). Lead and cadmium concentration in pike muscle tissue were significantly higher (P 〈 0.01, P 〈 0.05, respectively), and mercury and arsenic concentration were significantly lower (P 〈 0.01) than concentration of this heavy metals in carp muscle tissue in all comapered fish ponds. The results obtained in this study indicate variation in a range of examined metal concentration in muscle tissue, which seems to be typical for the Serbian fish ponds, but is lower than those in rivers with considerable anthropogenic impact.展开更多
A simple and rapid dispersive liquid-liquid microextraction(DLLME)technique coupled with gas chromatography-ion trap mass spectrometry(GC-MS)was developed for the extraction and analysis of five endosulfan pesticides ...A simple and rapid dispersive liquid-liquid microextraction(DLLME)technique coupled with gas chromatography-ion trap mass spectrometry(GC-MS)was developed for the extraction and analysis of five endosulfan pesticides from the fish pond water.In this work,different parameters affecting the extraction process such as the type and volume of extraction solvent,type and volume of disperser solvent,and extraction time were studied and optimized.Under optimized conditions,the enrichment factor ranged from 189 to 269 and the relative recovery ranged from 88.5%to 94.9%.The linear range was 2.0-80.0 mg/L;the limits of detection and quantitation were in the range 0.04-1.06 mg/L and 0.12-3.53 mg/L,respectively.The relative standard deviations were in the range 0.94%-2.08%(n D 5).The obtained results show that DLLME combined with GC-MS is a fast and simple method for the determination of endosulfan pesticides in fish pond water.展开更多
Rice-fish co-culture has gained increasing attention to remediate the negative environmental impacts induced by intensive aquaculture. However, the effect of rice-fish co-culture on oxygen depletion has rarely been in...Rice-fish co-culture has gained increasing attention to remediate the negative environmental impacts induced by intensive aquaculture. However, the effect of rice-fish co-culture on oxygen depletion has rarely been investigated. We constructed a rice-fish co-culture system in yellow catfish(Pelteobagrus fulvidraco) and freshwater shrimp(Macrobrachium nipponense) ponds using a new high-stalk rice variety, and conducted a field experiment to investigate the effect of rice-fish co-culture on water parameters and oxygen consumption. The results showed that rice-fish co-culture reduced the nutrients(total nitrogen, ammonia-N, total phosphorous and potassium) and the dissolved oxygen content in fish and shrimp ponds. However, they showed similar seasonal change of dissolved oxygen in the water of fish and shrimp ponds. Rice-fish co-culture reduced the total amount of oxygen consumption and optimized the oxygen consumption structure in pond. The respiration rates in water and sediment were significantly reduced by 66.1% and 31.7% in the catfish pond, and 64.4% and 38.7% in the shrimp pond, respectively, by additional rice cultivation. Rice-fish co-culture decreased the proportions of respiration in sediment and water, and increased the proportion of fish respiration. These results suggest that rice-fish co-culture is an efficient way to reduce hypoxia in intensive culture pond.展开更多
It is not understood well that how the effects of land use and fish farming on the contents of alkali metals (Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and Na<sup>+</...It is not understood well that how the effects of land use and fish farming on the contents of alkali metals (Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and Na<sup>+</sup>) in small water bodies of pond and reservoir ecosystems at the watershed scale. In this study, the concentrations of Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and Na<sup>+</sup> in water bodies were measured for 103 ponds and reservoirs used as fish farming or surrounded by different agricultural land use types in the subtropical hilly watershed of Jinjing (105 km<sup>2</sup>), China. The two important environmental factors of fishing farming and agricultural land use influenced the spatial variation the contents of alkali metals. The ponds and reservoirs in residential area had significantly higher concentrations of Ca<sup>2+</sup> and Na<sup>+</sup> than those with other land use types, reflecting the influence of domestic wastewater. Compared with those of natural ponds with non-fish farming, no significant increase of alkali metal contents occurred in fish farming ponds, due to the regular cleaning of ponds by farmers. However, the effect of fish culture on alkali metal contents was still supported indirectly by the fact that the alkali metal contents significantly correlated with nitrate contents in fish farming ponds and but high related with that of DIP in natural ponds. The suitability assessment for irrigation on the pond water indicated that almost all of ponds were suitable for irrigation except some ponds surrounded by residential area and tea plantation. Generally, our results demonstrated that fish farming and agricultural land use affected the contents of alkali metals in ponds and reservoirs. The agricultural water irrigation would be with caution from the ponds with tea plantation and residential area in the subtropical hilly watershed.展开更多
The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. H...The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. However, no research has been reported on the detailed calculation of the relationship between the area of aquaculture pond and purification pond. In this study, referring to the absorption ability of aquatic plants to pollutants in aquaculture wastewater and pollutant generation and discharge coefficient in aquaculture pond, based on the general rules of water quality management in freshwater aquaculture system, a calculation mode was es- tablished to investigate the relationship between the area of aquaculture pond and purification pond in freshwater recirculation aquaculture system, which was feasible to explain related cases and would provide theoretical basis to reduce the economic costs in the construction of water circulation aquaculture system and realize the bal- ance between the ecological benefits and the economic benefits.展开更多
The Hula Valley was drained in 1957. The land use was modified from natural wetland and old shallow lake ecosystems to agricultural development. About half of the drained land area was utilized for aquaculture. Popula...The Hula Valley was drained in 1957. The land use was modified from natural wetland and old shallow lake ecosystems to agricultural development. About half of the drained land area was utilized for aquaculture. Population size was enhanced and the diary was developed intensively resulting in the enhancement of domestic and husbandry sewage production that increased as well. The natural intact Hula Valley-Lake Kinneret ecosystem was heavily anthropogenically interrupted: The Hula was drained and Kinneret became a national source for domestic water supply. Some aspects of the environmental and water quality protection policy of the system are presented. The causation and operational management implications for the reduction of Nitrogen and Phosphorus migration from the Hula Valley are discussed. Drastic (81%) restriction of aquaculture accompanied by sewage totally removed achieved a reasonable improvement in pollution control which was also supported by the Hula Project. The implications of anthropogenic intervention in the process of environmental management design are presented.展开更多
A seven-month research evaluated the management effect on the water quality and sediment of seven fish ponds. Water and sediment were collected at nine sample sites: seven in the fish ponds;one in inlet water and anot...A seven-month research evaluated the management effect on the water quality and sediment of seven fish ponds. Water and sediment were collected at nine sample sites: seven in the fish ponds;one in inlet water and another in the fish farm’s effluent. The soil samples were analyzed for macro- and micro-nutrients and the water samples were analyzed for physical and chemical parameters. Management and local climate conditions affected nutrient seasonality in the sediment and featured high concentrations of Al, Ca, Cu, K, Mg, C, Na, Zn and OM at the effluent with low pH, ranging between 4.4 and 6.5. Sudden decrease of DO (less than 3 mg/L) during the rainy season, with a 180 mmrainfall, and a TSS increase (approximately 10 mg/L) were reported. Use of organic manure in fish pond V6 caused higher rates of ammonia (over 1 mg/L). Due to the sediment’s acid pH (less than 4.8) and Al at 0.92 mg/L at the effluent, great care was required in the fish farm. Maintenance and procedures management in the fish farm under analysis should be given more attention since high levels of Al, Fe and acid pH and low levels of potassium and phosphorus in the sediment may produce unfavorable conditions in the water column, and may ultimately have an impact on fish.展开更多
The practice of integrated fish farming and the use of local fish meal and manure for fish feeding in Cameroon constitute potential sources of resistant pathogenic bacteria in the fish pond environment.Therefore,a per...The practice of integrated fish farming and the use of local fish meal and manure for fish feeding in Cameroon constitute potential sources of resistant pathogenic bacteria in the fish pond environment.Therefore,a periodical and constant monitoring of the microbiological quality of fish pond is imperative.This study was to assess the microbial contamination of Mfou aquaculture production site and evaluate the antibiotic resistance profile of bacterial fish isolates.Samples of pond water(n=36),sediment(n=36),fishmeal(n=12)and African catfish(Clarias gariepinus),kanga(Heterotis niloticus)and Nile Tilapia(Oreochromis niloticus)(n=36,each)were collected to determine TVAC(Total Viable Aerobic Bacterial Count),FC(Fungal Count),SAC(Staphylococcus aureus count),TCC(Total Coliform Count)and FCC(Feacal Coliform Count).The fish skin isolates of S.aureus,Enterobacter sakazakii,Citrobacter freundii,Serratia fonticola,Klebsiella oxytoca,Proteus spp.,Aeromonas hydrophila,Kluyvera spp.,Moraxella spp.,Pasteurella multocida and Pseudomonas fluorescens were tested against penicillin G(10μg),chloramphenicol(30μg),sulfamethoxazole/trimethoprim(25μg),erythromycin(15μg),tetracycline(30μg),using the disk diffusion method.Results reveal a heavy contamination of fish farms with microbial load above the recommended limits.Our study indicates that fish ponds are sources of zoonotic pathogens underlining their epidemiological and clinical relevance.All bacterial isolates were multiresistant with a multiple antibiotic resistance index above 0.2.These data raise concern about the microbial contamination of aquaculture and associated public health risks in Cameroon.展开更多
Ecological stoichiometry of nitrogen and phosphorus is an important indicator to characterize the nitrogen and phosphorus trophic status in aquatic ecosystems. The study of the spatio-temporal patterns of nitrogen and...Ecological stoichiometry of nitrogen and phosphorus is an important indicator to characterize the nitrogen and phosphorus trophic status in aquatic ecosystems. The study of the spatio-temporal patterns of nitrogen and phosphorus stoichiometry is beneficial to the nitrogen and phosphorus pollution management in pond ecosystems. In this study, 18 groups (36 in total) of typical cascade ponds were selected as long-term observations to investigate the spatial distribution patterns of nitrogen and phosphorus component ratios (ratio of total nitrogen to phosphorus: TN:TP, ratio of dissolved nitrogen to phosphorus: TDN:TDP, ratio of particulate nitrogen to phosphorus: PN:PP) in water bodies in the tropical agricultural watershed of Jinjing. The results showed that the average values of TN:TP and TDN:TDP in the upstream ponds were 26.4 and 53.4, respectively, and were more than those in the downstream (22.95 and 48.1, respectively). In contrast, the PN:PP (13.78) in the upstream was significantly lower than that of the downstream (30.39). Furthermore, the factors of rainfall, agricultural land use and fish farming influenced the spatio-temporal variability of the N:P ratios. The ratios of TN:TP and TDN:TDP were higher in the wet season and lower in the dry season. Agricultural land use and fish farming reduced the ratios of the above three nitrogen and phosphorus components in cascade ponds in the study area. Our results show that strengthening agricultural land pollution control and aquaculture management could help to improve water quality of pond ecosystems in the study area.展开更多
Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersa...Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersaturation. This article describes a novel inlet structure that fulfills those requirements. In addition, it requires minimal maintenance, and allows for the quick and easy removal of debris in the event of partial plugging. This simple aluminum structure consists of a mount for attachment to the inflow pipe and a terminal splash plate with aeration holes. The splash plate is perpendicular to the water flow during normal operations to both prevent fish from jumping into the pipe and aerate the incoming water. However, it can be easily swiveled upward for the efficient removal of debris. Use of the inlet structure consistently decreased gas supersaturation in the spring water as it entered the fish production pond. By decreasing gas levels and maintaining water flows, potentially hazardous fish health issues can be avoided by using this structure. In addition, this relatively inexpensive and simple device will greatly reduce the labor required for removing debris compared to traditional screens.展开更多
文摘This study was conducted to determine the contamination data of some heavy metals in 10 fish tissue samples of two different fish species---common carp and Northern pike from four different fish ponds and to compare the level of contamination and the differences in the concentration of these elements between these two fish species and between compared fish ponds. In various fish ponds, metal concentrations in the carp muscle tissue varied depending on the metal type. Between compared fish ponds, there were significant differences (P 〈 0.01) in the concentration in muscle carp tissue, except for cadmium. Metal concentrations in the pike muscle tissue also varied among fish ponds, depending on the type of metal. In all compared cases, there were significant differences between metal concentration in pike muscle tissue (P 〈 0.01). Lead and cadmium concentration in pike muscle tissue were significantly higher (P 〈 0.01, P 〈 0.05, respectively), and mercury and arsenic concentration were significantly lower (P 〈 0.01) than concentration of this heavy metals in carp muscle tissue in all comapered fish ponds. The results obtained in this study indicate variation in a range of examined metal concentration in muscle tissue, which seems to be typical for the Serbian fish ponds, but is lower than those in rivers with considerable anthropogenic impact.
文摘A simple and rapid dispersive liquid-liquid microextraction(DLLME)technique coupled with gas chromatography-ion trap mass spectrometry(GC-MS)was developed for the extraction and analysis of five endosulfan pesticides from the fish pond water.In this work,different parameters affecting the extraction process such as the type and volume of extraction solvent,type and volume of disperser solvent,and extraction time were studied and optimized.Under optimized conditions,the enrichment factor ranged from 189 to 269 and the relative recovery ranged from 88.5%to 94.9%.The linear range was 2.0-80.0 mg/L;the limits of detection and quantitation were in the range 0.04-1.06 mg/L and 0.12-3.53 mg/L,respectively.The relative standard deviations were in the range 0.94%-2.08%(n D 5).The obtained results show that DLLME combined with GC-MS is a fast and simple method for the determination of endosulfan pesticides in fish pond water.
基金supported by the Natural Science Foundation of China(Grant No.31400379)Natural Science Foundation of Zhejiang Province of China(Grant No.LY15C030002)Innovation Program of Chinese Academy of Agricultural Sciences
文摘Rice-fish co-culture has gained increasing attention to remediate the negative environmental impacts induced by intensive aquaculture. However, the effect of rice-fish co-culture on oxygen depletion has rarely been investigated. We constructed a rice-fish co-culture system in yellow catfish(Pelteobagrus fulvidraco) and freshwater shrimp(Macrobrachium nipponense) ponds using a new high-stalk rice variety, and conducted a field experiment to investigate the effect of rice-fish co-culture on water parameters and oxygen consumption. The results showed that rice-fish co-culture reduced the nutrients(total nitrogen, ammonia-N, total phosphorous and potassium) and the dissolved oxygen content in fish and shrimp ponds. However, they showed similar seasonal change of dissolved oxygen in the water of fish and shrimp ponds. Rice-fish co-culture reduced the total amount of oxygen consumption and optimized the oxygen consumption structure in pond. The respiration rates in water and sediment were significantly reduced by 66.1% and 31.7% in the catfish pond, and 64.4% and 38.7% in the shrimp pond, respectively, by additional rice cultivation. Rice-fish co-culture decreased the proportions of respiration in sediment and water, and increased the proportion of fish respiration. These results suggest that rice-fish co-culture is an efficient way to reduce hypoxia in intensive culture pond.
文摘It is not understood well that how the effects of land use and fish farming on the contents of alkali metals (Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and Na<sup>+</sup>) in small water bodies of pond and reservoir ecosystems at the watershed scale. In this study, the concentrations of Ca<sup>2+</sup>, Mg<sup>2+</sup>, K<sup>+</sup> and Na<sup>+</sup> in water bodies were measured for 103 ponds and reservoirs used as fish farming or surrounded by different agricultural land use types in the subtropical hilly watershed of Jinjing (105 km<sup>2</sup>), China. The two important environmental factors of fishing farming and agricultural land use influenced the spatial variation the contents of alkali metals. The ponds and reservoirs in residential area had significantly higher concentrations of Ca<sup>2+</sup> and Na<sup>+</sup> than those with other land use types, reflecting the influence of domestic wastewater. Compared with those of natural ponds with non-fish farming, no significant increase of alkali metal contents occurred in fish farming ponds, due to the regular cleaning of ponds by farmers. However, the effect of fish culture on alkali metal contents was still supported indirectly by the fact that the alkali metal contents significantly correlated with nitrate contents in fish farming ponds and but high related with that of DIP in natural ponds. The suitability assessment for irrigation on the pond water indicated that almost all of ponds were suitable for irrigation except some ponds surrounded by residential area and tea plantation. Generally, our results demonstrated that fish farming and agricultural land use affected the contents of alkali metals in ponds and reservoirs. The agricultural water irrigation would be with caution from the ponds with tea plantation and residential area in the subtropical hilly watershed.
基金Supported by Special Fund for the Construction of Modern Agricultural Industry Technology System(CARS-46)Special Fund for Basic Research and Operating Expenses of Central-level Research Institutes(2007JBFA03)~~
文摘The establishment of water circulation aquaculture system realized the hi- erarchical use of nitrogen, phosphorus and other eutrophic substances in aquaculture wastewater and the recirculation use of water resource. However, no research has been reported on the detailed calculation of the relationship between the area of aquaculture pond and purification pond. In this study, referring to the absorption ability of aquatic plants to pollutants in aquaculture wastewater and pollutant generation and discharge coefficient in aquaculture pond, based on the general rules of water quality management in freshwater aquaculture system, a calculation mode was es- tablished to investigate the relationship between the area of aquaculture pond and purification pond in freshwater recirculation aquaculture system, which was feasible to explain related cases and would provide theoretical basis to reduce the economic costs in the construction of water circulation aquaculture system and realize the bal- ance between the ecological benefits and the economic benefits.
文摘The Hula Valley was drained in 1957. The land use was modified from natural wetland and old shallow lake ecosystems to agricultural development. About half of the drained land area was utilized for aquaculture. Population size was enhanced and the diary was developed intensively resulting in the enhancement of domestic and husbandry sewage production that increased as well. The natural intact Hula Valley-Lake Kinneret ecosystem was heavily anthropogenically interrupted: The Hula was drained and Kinneret became a national source for domestic water supply. Some aspects of the environmental and water quality protection policy of the system are presented. The causation and operational management implications for the reduction of Nitrogen and Phosphorus migration from the Hula Valley are discussed. Drastic (81%) restriction of aquaculture accompanied by sewage totally removed achieved a reasonable improvement in pollution control which was also supported by the Hula Project. The implications of anthropogenic intervention in the process of environmental management design are presented.
文摘A seven-month research evaluated the management effect on the water quality and sediment of seven fish ponds. Water and sediment were collected at nine sample sites: seven in the fish ponds;one in inlet water and another in the fish farm’s effluent. The soil samples were analyzed for macro- and micro-nutrients and the water samples were analyzed for physical and chemical parameters. Management and local climate conditions affected nutrient seasonality in the sediment and featured high concentrations of Al, Ca, Cu, K, Mg, C, Na, Zn and OM at the effluent with low pH, ranging between 4.4 and 6.5. Sudden decrease of DO (less than 3 mg/L) during the rainy season, with a 180 mmrainfall, and a TSS increase (approximately 10 mg/L) were reported. Use of organic manure in fish pond V6 caused higher rates of ammonia (over 1 mg/L). Due to the sediment’s acid pH (less than 4.8) and Al at 0.92 mg/L at the effluent, great care was required in the fish farm. Maintenance and procedures management in the fish farm under analysis should be given more attention since high levels of Al, Fe and acid pH and low levels of potassium and phosphorus in the sediment may produce unfavorable conditions in the water column, and may ultimately have an impact on fish.
文摘The practice of integrated fish farming and the use of local fish meal and manure for fish feeding in Cameroon constitute potential sources of resistant pathogenic bacteria in the fish pond environment.Therefore,a periodical and constant monitoring of the microbiological quality of fish pond is imperative.This study was to assess the microbial contamination of Mfou aquaculture production site and evaluate the antibiotic resistance profile of bacterial fish isolates.Samples of pond water(n=36),sediment(n=36),fishmeal(n=12)and African catfish(Clarias gariepinus),kanga(Heterotis niloticus)and Nile Tilapia(Oreochromis niloticus)(n=36,each)were collected to determine TVAC(Total Viable Aerobic Bacterial Count),FC(Fungal Count),SAC(Staphylococcus aureus count),TCC(Total Coliform Count)and FCC(Feacal Coliform Count).The fish skin isolates of S.aureus,Enterobacter sakazakii,Citrobacter freundii,Serratia fonticola,Klebsiella oxytoca,Proteus spp.,Aeromonas hydrophila,Kluyvera spp.,Moraxella spp.,Pasteurella multocida and Pseudomonas fluorescens were tested against penicillin G(10μg),chloramphenicol(30μg),sulfamethoxazole/trimethoprim(25μg),erythromycin(15μg),tetracycline(30μg),using the disk diffusion method.Results reveal a heavy contamination of fish farms with microbial load above the recommended limits.Our study indicates that fish ponds are sources of zoonotic pathogens underlining their epidemiological and clinical relevance.All bacterial isolates were multiresistant with a multiple antibiotic resistance index above 0.2.These data raise concern about the microbial contamination of aquaculture and associated public health risks in Cameroon.
文摘Ecological stoichiometry of nitrogen and phosphorus is an important indicator to characterize the nitrogen and phosphorus trophic status in aquatic ecosystems. The study of the spatio-temporal patterns of nitrogen and phosphorus stoichiometry is beneficial to the nitrogen and phosphorus pollution management in pond ecosystems. In this study, 18 groups (36 in total) of typical cascade ponds were selected as long-term observations to investigate the spatial distribution patterns of nitrogen and phosphorus component ratios (ratio of total nitrogen to phosphorus: TN:TP, ratio of dissolved nitrogen to phosphorus: TDN:TDP, ratio of particulate nitrogen to phosphorus: PN:PP) in water bodies in the tropical agricultural watershed of Jinjing. The results showed that the average values of TN:TP and TDN:TDP in the upstream ponds were 26.4 and 53.4, respectively, and were more than those in the downstream (22.95 and 48.1, respectively). In contrast, the PN:PP (13.78) in the upstream was significantly lower than that of the downstream (30.39). Furthermore, the factors of rainfall, agricultural land use and fish farming influenced the spatio-temporal variability of the N:P ratios. The ratios of TN:TP and TDN:TDP were higher in the wet season and lower in the dry season. Agricultural land use and fish farming reduced the ratios of the above three nitrogen and phosphorus components in cascade ponds in the study area. Our results show that strengthening agricultural land pollution control and aquaculture management could help to improve water quality of pond ecosystems in the study area.
文摘Structures separating fish hatchery ponds from open spring water sources must restrict fish movement from the pond to the spring, allow for constant water flow, and potentially reduce incoming spring water gas supersaturation. This article describes a novel inlet structure that fulfills those requirements. In addition, it requires minimal maintenance, and allows for the quick and easy removal of debris in the event of partial plugging. This simple aluminum structure consists of a mount for attachment to the inflow pipe and a terminal splash plate with aeration holes. The splash plate is perpendicular to the water flow during normal operations to both prevent fish from jumping into the pipe and aerate the incoming water. However, it can be easily swiveled upward for the efficient removal of debris. Use of the inlet structure consistently decreased gas supersaturation in the spring water as it entered the fish production pond. By decreasing gas levels and maintaining water flows, potentially hazardous fish health issues can be avoided by using this structure. In addition, this relatively inexpensive and simple device will greatly reduce the labor required for removing debris compared to traditional screens.