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Second-order calibration applied to quantification of two active components of Schisandra chinensis in complex matrix 被引量:1
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作者 Xiao-Hua Zhang, Hai-Long Wu, Jian-Yao Wang, Yao Chen, Yong-Jie Yu, Chong-Chong Nie, Chao Kang, De-Zhu Tu, Ru-Qin Yu State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2012年第4期241-248,共8页
The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis f... The effectiveness of traditional Chinese medicine (TCM) against various diseases urges more low cost, speed and sensitive analytical methods for investigating the phamacology of TCM and providing a theoretical basis for clinical use. The potential of second-order calibration method was validated for the quantification of two effective ingredients of Schisandra chinensis in human plasma using spectrofluorimetry. The results obtained in the present study demonstrate the advantages of this strategy for multi-target determination in complex matrices. Although the spectra of the analytes are similar and a large number of interferences also exist, second-order calibration method could predict the accurate concentrations together with reasonable resolution of spectral profiles for analytes of interest owing to its ‘second-order advantage’. Moreover, the method presented in this work allows one to simply experimental procedure as well as reduces the use of harmful chemical solvents. 展开更多
关键词 Traditional Chinese medicine Second-order calibration Schizandrol A Schizandrin B Self-weighted alternating normalized residue fitting (SWANRF) algorithm Alternating normaliza- tion-weighted error (ANWE) algorithm.
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