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Anthocyanin profi les and color properties of red wines made from Vitis davidii and Vitis vinifera grapes 被引量:8
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作者 Yanlun Ju La Yang +5 位作者 Xiaofeng Yue Yunkui Li Rui He Shenglin Deng Xin Yang Yulin Fang 《Food Science and Human Wellness》 SCIE 2021年第3期335-344,共10页
Spine grape(Vitis davidii Foex.)is an important wild grape species native to China.Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin p... Spine grape(Vitis davidii Foex.)is an important wild grape species native to China.Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin profiles and color properties of wines made from V.davidii and V.vinifera grapes.Among spine wines,‘Junzi#2’wine had the highest total phenolic and total anthocyanin,‘Xiangzhenzhu’wine had the highest total flavonoids,and‘Junzi#1’wine had the highest total tannin.The anthocyanin compositions of all of the spine wines were dominated by Mv-3,5-diglucoside.The total individual anthocyanin contents in spine wines,except‘Gaoshan#5’,‘Junzi#5055’,‘Junzi#5061’,and‘Junzi#5044’,were signifi cantly higher than in V.vinifera wines.Most of the spine wines had a stronger red intensity and a brighter chroma with a bluer hue than V.vinifera wines.Correlation analysis revealed that the color properties were closely related to the anthocyanin composition.These results suggest that wines made from spine grapes may be useful for the wine industry for their color properties and high individual anthocyanin contents. 展开更多
关键词 Spine grape vitis vinifera l. Wine analysis Wine color ANTHOCYANIN
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Somatic embryogenesis and histological analysis from zygotic embryos in Vitis vinifera L.‘Moldova’ 被引量:3
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作者 LI Hui LI Feng-lan +2 位作者 DU Jing-chuan LU Hai HE Zheng-quan 《Forestry Studies in China》 CAS 2008年第4期253-258,共6页
We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape 'Moldova' (Vitis vinifera U 'Moldova'). Primary calli were initiated on Nitsch and Nitsch (NN) ... We examined the somatic embryogenesis from and histological studies of zygotic embryos of seeds in European Grape 'Moldova' (Vitis vinifera U 'Moldova'). Primary calli were initiated on Nitsch and Nitsch (NN) medium supplemented with 1.0 mg·L^-1 2,4-D and 0.5 mg·L^-1 6-BA. Embryogenic calli were produced upon transfer to a NN medium with 0.5 mg·L^-1 6-BA and 2 mg·L^-1 NAA and somatic embryos were obtained on a half strength MS medium without plant growth regulators. During the somatic embryo germination, an addition of 1.0 mg·L^-1 6-BA in the medium could accelerate somatic embryos to develop into normal plants and increase the conversion rate from 0 to 43.3%. Histological studies of embryogenic calli and somatic embryos demonstrated dynamic changes of proteins and starch grains. The developmental processes of somatic embryos were similar to those of zygotic embryos, including typical epiderma, cotyledon primordium and vascular tissue. 展开更多
关键词 zygotic embryo somatic embryo HISTOlOGY vitis vinifera l.
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葡萄(Vitis vinifera L.)试管繁殖中芽增殖能力衰减的原因及其防治方法的研究
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作者 范子南 肖华山 曾焕泰 《福建师范大学学报(自然科学版)》 CAS CSCD 1992年第4期74-79,共6页
以20个葡萄品种试管苗作为实验材料,经四年、四十多代的继代培养反复对比实验发现:在葡萄试管繁殖中,连续恒温(25±1℃)条件下培养或只采用固体培养方法,不适期进行继代培养均会导致芽增殖能力的衰减,根据生物统计分析,当继代培养超... 以20个葡萄品种试管苗作为实验材料,经四年、四十多代的继代培养反复对比实验发现:在葡萄试管繁殖中,连续恒温(25±1℃)条件下培养或只采用固体培养方法,不适期进行继代培养均会导致芽增殖能力的衰减,根据生物统计分析,当继代培养超过16~17代后,芽增殖能力即随着继代培养次数的增加而逐渐下降,采取变温培养、变换培养方法、适期继代培养或在增殖培养基中附加适量的椰子乳汁等方法均可防治芽增殖能力的哀减,保持高的芽增殖能力。 展开更多
关键词 葡萄 组织培养 芽增殖
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葡萄(Vitis Vinifera L.)X-射线能谱元素微区污染分析研究
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作者 周劲松 杨海燕 《青海大学学报(自然科学版)》 2002年第3期6-7,共2页
对葡萄果实不同处理的细胞组织多位点进行X -射线能谱微区元素分析。结果 :葡萄不同处理的细胞组织中共发现Mg、Al、Si、P、S、Cl、K、Ca、Fe、Cu十种元素。且不同处理的组织细胞中各元素含量分布有差异 ,未清洗果实表皮处理的Al、Si、S... 对葡萄果实不同处理的细胞组织多位点进行X -射线能谱微区元素分析。结果 :葡萄不同处理的细胞组织中共发现Mg、Al、Si、P、S、Cl、K、Ca、Fe、Cu十种元素。且不同处理的组织细胞中各元素含量分布有差异 ,未清洗果实表皮处理的Al、Si、S、Ca、Cu含量均显著高于对照 。 展开更多
关键词 污染分析 葡萄 果实污染 X-射线能谱微区分析法 重金属化学污染 元素分析
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The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L. 被引量:3
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作者 ZHAO Feng-xia CHEN Shang-wu +3 位作者 Perl Avihai DAI Ru XU Hai-ying MA Hui-qin 《Agricultural Sciences in China》 CAS CSCD 2011年第5期686-694,共9页
Non-embryogenic calli (NEC) was inevitably and heavily produced when grape embryogenic calli (EC) was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform ... Non-embryogenic calli (NEC) was inevitably and heavily produced when grape embryogenic calli (EC) was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and EC.In this research,a vector pA5 containing a chitinase signal sequence fused to gfp (green fluorescent protein) and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera L.cv.Chardonnay NEC.It was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone (AS) was optimized as protocol.The use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher efficiency.Kanamycin at 200 mg L-1 was picked for positive expression selection.The stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques (RT-PCR) and fluorescent microscopy after three sub-cultures of the selected cell line.Highly efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for EC.This transformation platform could facilitate the verification of candidate somatic embryogenesis (SE) decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition. 展开更多
关键词 Agrobacterium tumefaciens non-embryogenic calli TRANSFORMATION vitis vinifera l. RT-PCR
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The potential role of grape (Vitis vinifera L.) in prevention of threatened abortion via immunomodulatory and anti-inflammatory abilities: a hypothesis 被引量:4
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作者 Malihe Tabarrai Mozhgan Mehriardestani +2 位作者 Sharareh Hekmat Fatemeh Nejatbakhsh Fatemeh Moradi 《Traditional Medicine Research》 2019年第3期131-139,共9页
Threatened abortion is a common problem in early pregnancy. This early vaginal bleeding happens in about 25% of pregnant women. The medications including progesterone, uterine muscle relaxant or human chorionic gonado... Threatened abortion is a common problem in early pregnancy. This early vaginal bleeding happens in about 25% of pregnant women. The medications including progesterone, uterine muscle relaxant or human chorionic gonadotropin have essential effects in developing pregnancy, but the clinical data are insufficient to prescribe them. In recent decades, medicinal herbs can help us to present new treatments. Grape (Vitis vinifera L.) can protect the fetus from the perspective of Persian medicine. So, we hypothesize about the salutary effects of grape in miscarriage prevention. We found five standard expected mechanisms of grape to prevent threatened abortion: immunomodulatory and anti-inflammatory, anti-oxidant, anti-contraction, hormonal and anti-stress activities. Grape reduces nitric oxide, prostaglandin E2, expression of nuclear factor κB and other pro-inflammatory cytokines like IL (Interleukin)-1β, IL-6, IL-8, and tumor necrosis factor-α. It also elevates anti-inflammatory mediators and expression of peroxisome proliferator-activated receptor-γ. Grape polyphenols have a crucial role in fetus protection with high antioxidant power and other functions such as prevention of stress-triggered abortion with proanthocyanidins, or hormonal effects and inhibition of uterine contractions with resveratrol. So according to these studies, grape probably has effects on the immune and endocrine factors involved in threatened miscarriage. 展开更多
关键词 vitis vinifera l. GRAPE Oxidation Immunomodulation Inflammation ABORTION PERSIAN medicine
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Rooting Abilities in <i>Vitis vinifera</i>L.: Total Lipid and Fatty Acids Evolution in Perle Noir Cutting
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作者 Henda Cheikhrouhou Manel Zrida Bechir Ezzili 《Agricultural Sciences》 2015年第5期498-504,共7页
Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out b... Rooting ability in Vitis vinifera L. is the phenomena studied. The evolution of the total lipids contents and fatty acids amount of cuttings during the rooting were determined. Total lipid extraction was carried out by an extraction solvent consisting of a mixture of chloroform and methanol. Methylation of fatty acids was carried out by the boron trifluoride methanol complex. Their analysis is performed by gas chromatography. We have obtained a content of merithallus lipids equivalent with then in bibliography. In other hand 3 fatty acids were obtained: eicosapentaenoic acid, oleic acid and docosanoic acid. Among the studied of rooting abilities, we observed decreased levels of total lipids and in the essential fatty acids: eicosapentaenoic acid C20:5 n-3 and oleic acid C18:1 n-9. Minor fatty acids cuttings experimental have a variable evolution. Lipids and certain fatty acids may be markers of rooting in the vine. 展开更多
关键词 lipids CUTTING Fatty Acids ROOTING Perle Noir vitis vinifera l.
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The Influence of Photoperiod on the Regulation of Root and Callus Initiation of Perle Noir (<i>V. vinifera</i>L.): Expression of MADS-Box Gene
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作者 Henda Cheikhrouhou Manel Zrida Bechir Ezzili 《Agricultural Sciences》 2015年第9期908-915,共8页
To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day lengt... To study the influence of photoperiod on roots differentiation in the Tunisian grapevine (Vitis vinifera L.) cultivar Perle noir, roots and callus initiation were analyzed under three different conditions of day length: long day (LD), short day (SD) and darkness (D). The photoperiod influenced the number of callus and roots per cuttings;it has a significant effect on the roots and callus initiation. Expression profile analysis of six MADS-box genes (VTM8, VSEP2, VAG12, VAG17-1, VAG17-2 and VSOC1.3) during root and callus development is in agreement with the above-mentioned observation. The expression of the MADS-box genes during root and callus development fluctuated in a tissue-dependent manner. These data suggest that all genes are expressed in roots under three photoperiods. Total darkness gives the number of the most important root per cutting compared to the other two conditions. This photoperiodic condition gave the most important expression of the studied genes VAG12, VAG17-2, VAG17-1, VTM8 and VSEP2 transcripts were not found in callus grown in the dark or in LD conditions, respectively. VSOC1.3 transcripts were not found in callus grown in the dark or in SD conditions, respectively. Transcript abundance of VTM8 and VSOC1 was highest in LD. 展开更多
关键词 Grapevine ROOT PHOTOPERIOD MADS-Box Genes CAllUS vitis vinifera l.
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Effects of mesoclimate and microclimate variations mediated by high altitude and row orientation on sucrose metabolism and anthocyanin synthesis in grape berries
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作者 Haining Yin Zhaoxiang Wang +3 位作者 Lin Wang Jianhong Cao Jiakui Wang Zhumei Xi 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期713-731,共19页
Climate change and extreme weather pose significant challenges to the traditional viticulture regions.Emerging high-altitude grape-producing regions with diverse orientations have shown great potential in coping with ... Climate change and extreme weather pose significant challenges to the traditional viticulture regions.Emerging high-altitude grape-producing regions with diverse orientations have shown great potential in coping with this challenge.Stable,high-quality wine grape production may be achieved by synchronizing the meso-and microclimate.To clarify the role of high altitude and row orientation in meso-and microclimate and the response of berries to it,we evaluated seven years(2012-2018)of climate data,two years of basic grape(Cabernet Sauvignon,Vitis vinifera L.)quality,and one-year microclimate from veraison to harvest.By comparing two locations(Sidon 2047 m,Sinon 2208 m)in Yunnan Province,China,we found that the average temperature has been stable at approximately 15℃ for seven years,with no extreme weather or,noticeable global warming.The light intensity(LI)in the north-south(NS)was more balanced than the east-west(EW)direction,and the east-west to the south(EW-S)canopy side was almost higher than the other sides.High LI was associated with high photosynthetically active radiation(PAR),ultraviolet(UV),and infrared(IR)light and vice versa.The north-south to the east(NS-E)and east-west to the north(EWN)sides were characterized by lower LI and higher UV and IR light,and higher total anthocyanin content.Most anthocyanin synthesis-related genes,for example,VvF3'H and VvF3'5'H,were highly expressed in NS-E from veraison to maturity.Perhaps UV and IR light induced their expression.This study provides new insights on the role of differently orientated rows in controlling grape quality due to varied light quality.The findings are globally significant,particularly in the context of climate change,and offer fresh insights into berry physiological responses and decision-making for the management of existing vineyards. 展开更多
关键词 vitis vinifera l. Orientated rows Berry composition SUGAR PHENOlIC light quality Solar radiation
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葡萄AFLP技术体系建立及其在超藤与藤稔葡萄品种鉴别中的应用 被引量:21
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作者 鲍露 徐昌杰 +2 位作者 江文彬 陈履荣 陈昆松 《果树学报》 CAS CSCD 北大核心 2005年第4期422-425,共4页
超藤葡萄是藤稔葡萄的一个芽变品种,由于2者亲缘关系十分接近,应用形态学手段以及RAPD分子标记不易区分。针对葡萄叶片富含多糖和多酚类等杂质的特点,从基因组DNA纯化入手,对AFLP流程进行了优化改进,建立了葡萄AFLP体系,并成功地对此2... 超藤葡萄是藤稔葡萄的一个芽变品种,由于2者亲缘关系十分接近,应用形态学手段以及RAPD分子标记不易区分。针对葡萄叶片富含多糖和多酚类等杂质的特点,从基因组DNA纯化入手,对AFLP流程进行了优化改进,建立了葡萄AFLP体系,并成功地对此2个品种进行了区分。采用引物组合M-CAT+E-ACG进行AFLP扩增,超藤葡萄出现167bp特异带。 展开更多
关键词 葡萄 AFlP 品种鉴别
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葡萄PYL基因家族的鉴定与表达分析 被引量:13
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作者 马宗桓 陈佰鸿 +1 位作者 李文芳 毛娟 《果树学报》 CAS CSCD 北大核心 2018年第3期265-274,共10页
【目的】通过分析葡萄(Vitis vinifera L.)PYL基因在非生物胁迫条件下的响应情况,丰富PYR/PYL/RCAR(Pyrabactin Resistance/Pyr1-Like/Regulatory Components of ABA Receptor)在ABA信号和启动信号转导中的功能。【方法】利用生物信息... 【目的】通过分析葡萄(Vitis vinifera L.)PYL基因在非生物胁迫条件下的响应情况,丰富PYR/PYL/RCAR(Pyrabactin Resistance/Pyr1-Like/Regulatory Components of ABA Receptor)在ABA信号和启动信号转导中的功能。【方法】利用生物信息学方法,筛选葡萄中的PYL基因,并对其进行生物信息学及非生物胁迫下的响应分析。【结果】从葡萄基因组中共鉴定出6个PYL基因,Vv PYL家族无染色体偏好性,Vv PYL5无内含子结构;除Vv PYL4外均富含酸性氨基酸,该家族成员主要定位于细胞质中,并有多个保守位点。10%(ω)PEG和100μmol·L^(-1)ABA处理6 h后,Vv PYL1和Vv PYL2表达水平与对照相比差异显著,分别为对照的1.3~1.8倍。50μmol·L^(-1)ABA处理6 h后,Vv PYL1表达水平与对照差异显著,为对照的1.5倍;400 mmol·L^(-1)Na Cl、10%PEG、50μmol·L^(-1)ABA和100μmol·L^(-1)ABA处理24 h后,Vv PYL1和Vv PYL2的表达水平显著高于对照,为对照的1.2~2.2倍,400 mmol·L^(-1)Na Cl处理24 h后,Vv PYL6的表达水平显著高于对照,为对照的1.6倍。【结论】克隆得到葡萄的6个PYL基因,高度保守,分为3个亚组;能够响应不同非生物胁迫。本研究为葡萄PYL基因在逆境应答中的功能研究提供了基础。 展开更多
关键词 葡萄 PYR/PYl/RCAR家族 生物信息学 实时荧光定量PCR
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利用ISSR和叶绿体trnL-trnF序列变异研究葡萄种质资源的遗传多样性和系统发育关系 被引量:4
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作者 王蕾 张娟 +4 位作者 刘林德 张莉 魏丽娟 胡德昌 邓世斌 《鲁东大学学报(自然科学版)》 2015年第1期32-38,43,共8页
利用ISSR标记和叶绿体DNA trn L-trn F非编码区分析36种葡萄种质的遗传多样性和系统发育关系.13个ISSR引物共产生了63条带,平均有效等位基因数、Nei's基因多样性和香农信息指数分别是1.5827,0.3463和0.5172.基于UPGMA的ISSR分析将... 利用ISSR标记和叶绿体DNA trn L-trn F非编码区分析36种葡萄种质的遗传多样性和系统发育关系.13个ISSR引物共产生了63条带,平均有效等位基因数、Nei's基因多样性和香农信息指数分别是1.5827,0.3463和0.5172.基于UPGMA的ISSR分析将葡萄种质材料分为6个主要的进化枝.葡萄在trn L-trn F区域有较高的遗传多样性水平(Hd=0.667,Pi=0.00206).由NJ,ML和贝叶斯分析得到的进化树都没有表现出不同地区葡萄品种与地域之间的地理相关性.中性检验葡萄的进化历史过程中发生过种群扩张事件.葡萄种质资源的保护应以就地保护为主.本研究为葡萄种质资源的理论和应用研究提供了有价值的信息. 展开更多
关键词 葡萄 ISSR trn l-trn F非编码区 遗传多样性 遗传关系
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葡萄F-box基因VvSLY1的克隆与表达分析 被引量:1
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作者 王西成 吴伟民 +3 位作者 王壮伟 赵密珍 周蓓蓓 钱亚明 《植物遗传资源学报》 CAS CSCD 北大核心 2018年第5期959-966,共8页
SLEEPY1(SLY1)属于F-box蛋白,是SCF复合体的主要组成元件之一,在赤霉素信号转导过程中发挥着重要的调节作用。本研究以夏黑葡萄为试材,利用电子克隆和RT-PCR技术克隆获得一个F-box蛋白基因全长c DNA序列,命名为VvSLY1。该基因全长为1096... SLEEPY1(SLY1)属于F-box蛋白,是SCF复合体的主要组成元件之一,在赤霉素信号转导过程中发挥着重要的调节作用。本研究以夏黑葡萄为试材,利用电子克隆和RT-PCR技术克隆获得一个F-box蛋白基因全长c DNA序列,命名为VvSLY1。该基因全长为1096 bp,包含1个555 bp的完整开放阅读框(ORF),编码184个氨基酸。序列比对和结构域分析结果表明,该VvSLY1包含F-box蛋白家族的LGG和LSL保守结构域。进化树分析结果显示,VvSLY1与可可亲缘关系最近。qRTPCR分析结果表明,在果实快速膨大发育阶段,外源赤霉素GA3处理会促进该基因的上调表达。 展开更多
关键词 葡萄 F-BOX蛋白 VvSlY1基因 克隆 基因表达
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VitisMod:一个葡萄基因共表达数据库
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作者 刘伟 魏晓玲 何华勤 《生物信息学》 2023年第1期60-67,共8页
葡萄转录组已在各种组织、发育阶段、生物胁迫、非生物胁迫和其他条件下被测定。目前,仍没有简单实用的网络工具来探索这些宝贵的数据。本文从美国国立生物技术信息中心的基因表达数据库(NCBI GEO)下载1019个基因表达芯片数据,进行权重... 葡萄转录组已在各种组织、发育阶段、生物胁迫、非生物胁迫和其他条件下被测定。目前,仍没有简单实用的网络工具来探索这些宝贵的数据。本文从美国国立生物技术信息中心的基因表达数据库(NCBI GEO)下载1019个基因表达芯片数据,进行权重基因共表达网络分析(WGCNA)。鉴定到41个基因共表达模块。功能富集分析发现这些模块具有不同的功能,并与实验/表型相关。通过模块内连接度筛选枢纽基因,这些基因可能具有重要功能。通过关联推定(Guilt-by-association)原理对模块内功能未知的基因进行功能预测。最后,构建了免费的网络工具VitisMod,为葡萄的基因功能研究提供新资源,网址为:http://bioinformatics.fafu.edu.cn/grape。 展开更多
关键词 基因共表达 枢纽基因 连接度 葡萄 网络工具
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葡萄VvAGL11和VvAGL15基因的鉴定及其在赤霉素诱导葡萄无核果实发育过程中的作用 被引量:10
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作者 崔梦杰 郭凤菲 +3 位作者 王晨 纠松涛 朱旭东 房经贵 《南京农业大学学报》 CAS CSCD 北大核心 2019年第2期261-269,共9页
[目的]本文旨在研究VvAGL11和VvAGL15在应答赤霉素(GA3)信号诱导葡萄无核果实发育过程中的作用。[方法]以‘巨峰’葡萄为试材,鉴定了VvAGL11和VvAGL15的c DNA全长序列,并通过分析基因的启动子顺式作用元件预测其潜在功能,同时运用RT-qPC... [目的]本文旨在研究VvAGL11和VvAGL15在应答赤霉素(GA3)信号诱导葡萄无核果实发育过程中的作用。[方法]以‘巨峰’葡萄为试材,鉴定了VvAGL11和VvAGL15的c DNA全长序列,并通过分析基因的启动子顺式作用元件预测其潜在功能,同时运用RT-qPCR方法检测对照和GA3处理组葡萄VvAGL11和VvAGL15的时空表达水平。[结果]GA3处理能够诱导葡萄果实无核化,使葡萄果粒和果穗显著伸长,穗轴增粗。同时,鉴定到VvAGL11(MG581423)和VvAGL15(MG581424)的c DNA全长序列。VvAGL11和VvAGL15蛋白序列与可可和棉花的亲缘关系较近;二者均含有MADS-MEF2-like和K-box家族保守结构域,属于MADS-box转录因子家族;其主要二级结构元件为α-螺旋和无规则卷曲,且主要定位于细胞核中,蛋白结构具有保守性。启动子顺式作用元件分析显示:二者均含有响应赤霉素和胚乳发育相关的motifs。RT-qPCR分析显示,在果实硬核期和转色期的种子中,VvAGL11和VvAGL15的表达量较高,而GA3处理能够显著抑制其在种子中的表达。[结论]鉴定到MADS-box基因家族中的2个成员,分别为VvAGL11和VvAGL15。GA3处理可抑制VvAGL11和VvAGL15在种子区的表达,影响种胚的正常发育,从而形成无核果实。 展开更多
关键词 葡萄 VvAGl11 VvAGl15 克隆 种胚发育
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Impact of a Non-<i>Saccharomyces</i>Yeast Isolated in the Equatorial Region in the Acceptance of Wine Aroma 被引量:12
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作者 Mariana Oliveira Assis Adriana Pereira Coelho Santos +1 位作者 Carlos Augusto Rosa Maria Eugênia de Oliveira Mamede 《Food and Nutrition Sciences》 2014年第9期759-769,共11页
The aim of this work was to isolate and identify the yeasts prevalent in fresh grapes cultivated in the “S&atilde;o Francisco Valley” region (Brazil), as well as evaluating the cell growth of these indigenous ye... The aim of this work was to isolate and identify the yeasts prevalent in fresh grapes cultivated in the “S&atilde;o Francisco Valley” region (Brazil), as well as evaluating the cell growth of these indigenous yeasts during the fermentation of grape musts and their contribution to the improvement of wine aroma. The Chenin Blanc grape must fermented by H. opuntiae presented higher acceptance means at the three points analyzed (6.74, 6.78 and 7.30) and in the fermentation carried out by the yeasts H. opuntiae and S. cerevisiae, the highest mean acceptance (7.22) was observed after 120 hours, with no statistical difference from the sample fermented by H. opuntiae alone. Since these samples that showed higher acceptance means also receiving higher scores for purchasing intention, corresponding to the concepts of “definitely would buy” and “probably would buy”. The present study suggests that the fermentations of grape musts carried out by the yeast H. opuntiae and mixed cultures of H. opuntiae and Saccharomyces cerevisiae, positively influenced the sensory qualities of the wines and showed greater potential to increase the aroma of the musts and to develop specific wine styles. 展开更多
关键词 Aroma Fermentation NON-SACCHAROMYCES SACCHAROMYCES CEREVISIAE Variety Bayanus Sensory Analysis vitis vinifera l.
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Review of natural products with hepatoprotective effects 被引量:11
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作者 Eduardo Madrigal-Santillán Eduardo Madrigal-Bujaidar +7 位作者 Isela álvarez-González María Teresa Sumaya-Martínez José Gutiérrez-Salinas Mirandeli Bautista ángel Morales-González Manuel García-Luna y González-Rubio J Leopoldo Aguilar-Faisal José A Morales-González 《World Journal of Gastroenterology》 SCIE CAS 2014年第40期14787-14804,共18页
The liver is one of the most important organs in the body,performing a fundamental role in the regulationof diverse processes,among which the metabolism,secretion,storage,and detoxification of endogenous and exogenous... The liver is one of the most important organs in the body,performing a fundamental role in the regulationof diverse processes,among which the metabolism,secretion,storage,and detoxification of endogenous and exogenous substances are prominent.Due to these functions,hepatic diseases continue to be among the main threats to public health,and they remain problems throughout the world.Despite enormous advances in modern medicine,there are no completely effective drugs that stimulate hepatic function,that offer complete protection of the organ,or that help to regenerate hepatic cells.Thus,it is necessary to identify pharmaceutical alternatives for the treatment of liver diseases,with the aim of these alternatives being more effective and less toxic.The use of some plants and the consumption of different fruits have played basic roles in human health care,and diverse scientific investigations have indicated that,in those plants and fruits so identified,their beneficial effects can be attributed to the presence of chemical compounds that are called phytochemicals.The present review had as its objective the collecting of data based on research conducted into some fruits(grapefruit,cranberries,and grapes)and plants[cactus pear(nopal)and cactus pear fruit,chamomile,silymarin,and spirulina],which are consumed frequently by humans and which have demonstrated hepatoprotective capacity,as well as an analysis of a resin(propolis)and some phytochemicals extracted from fruits,plants,yeasts,and algae,which have been evaluated in different models of hepatotoxicity. 展开更多
关键词 HEPATOPROTECTION GRAPEFRUIT Vaccinium spp vitis vinifera l Opuntia ficus-indica SPIRUlINA PROPOlIS GlUCANS Alcoholic liver disease
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FeCl_3溶液引起葡萄白藜芦醇积累与Halliwell-Asada途径的偶联关系
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作者 董锦蕾 李月荣 +1 位作者 王晓琴 张波 《新疆农业科学》 CAS CSCD 北大核心 2017年第9期1713-1720,共8页
【目的】FeCl_3溶液可导致葡萄白藜芦醇积累但机制不明,研究Halliwell-Asada(H-A)途径与白藜芦醇积累的关系在损伤保护角度。【方法】FeCl_3溶液处理葡萄叶片后,检测细胞损伤程度和活性氧水平,分析白藜芦醇与H-A途径相关基因表达变化规... 【目的】FeCl_3溶液可导致葡萄白藜芦醇积累但机制不明,研究Halliwell-Asada(H-A)途径与白藜芦醇积累的关系在损伤保护角度。【方法】FeCl_3溶液处理葡萄叶片后,检测细胞损伤程度和活性氧水平,分析白藜芦醇与H-A途径相关基因表达变化规律。【结果】FeCl_3溶液造成叶片活性氧积累与叶片损伤正相关,且伴随白藜芦醇呈量效关系积累,峰值36.83μg/g;白藜芦醇合成途径以及H-A途径下相关酶PAL、C_4H、4CL、STS、APX和MDAR的基因表达量除DHAR基因外均明显上调;H_2O_2协同处理增加白藜芦醇含量,抗氧化剂NAC和GSH-EE协同则相反。【结论】Fe Cl3溶液降低葡萄叶片H-A途径对活性氧的清除能力,造成氧化胁迫并引起白藜芦醇积累,提示白藜芦醇合成与H-A途径存在偶联。 展开更多
关键词 白藜芦醇 细胞损伤 H-A途径 ROS积累 美国红地球葡萄
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Ectopic expression of VvFUS3,B3-domain transcription factor,in tomato influences seed development via affecting endoreduplication and hormones 被引量:1
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作者 Bilal Ahmad Songlin Zhang +6 位作者 Jin Yao Shengyue Chai Vivek Yadav Habib-ur-Rehman Athar Mati Ur Rahman Li Wang Xiping Wang 《Horticultural Plant Journal》 SCIE CSCD 2022年第3期351-360,共10页
FUSCA3(FUS3)is a member of B3-domain transcription factor family and master regulator of seed development.It has potential roles in hormone biosynthesis and signaling pathways and therefore plays diverse roles in plan... FUSCA3(FUS3)is a member of B3-domain transcription factor family and master regulator of seed development.It has potential roles in hormone biosynthesis and signaling pathways and therefore plays diverse roles in plant life cycle,especially in seed germination,dormancy,embryo formation,seed and fruit development,and maturation.However,there is limited information about its functions in seed and fruit development of grapevine.In this study,we expressed VvFUS3 in tomato for its functional characterization.Overexpression of VvFUS3 in tomato led to a reduction in seed number and seed weight without affecting the fruit size.Histological analysis found that both cell expansion and cell division in transgenic seed and fruit pericarp have been affected.However,there were no obvious differences in pollen size,shape,and viability,suggesting that VvFUS3 affects seed development but not the pollen grains.Moreover,the expression of several genes with presumed roles in seed development and hormone signaling pathways was also influenced by VvFUS3.These results suggest that VvFUS3 is involved in hormonal signaling pathways that regulate seed number and size.In conclusion,our study provides novel preliminary information about the pivotal roles of VvFUS3 in seed and fruit development and these findings can potentially serve as a reference for molecular breeding of seedless grapes. 展开更多
关键词 vitis vinifera l. ABI3 B3 transcription factor Seed number ENDOREDUPlICATION HORMONE
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Grapevine Phenological Quantitative Trait SSR Genotyping Using High-Throughput HRM-PCR Analysis
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作者 Murad Awad Photini V.Mylona Alexios N.Polidoros 《Phyton-International Journal of Experimental Botany》 SCIE 2020年第4期905-923,共19页
Discrimination among grapevine varieties based on quantitative traits,such as flowering,veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs.These trai... Discrimination among grapevine varieties based on quantitative traits,such as flowering,veraison and ripening dates is crucial for variety selection in the context of climate change and in breeding programs.These traits are under complex genetic control for which 6 linked SSR loci(VVS2,VVIn16,VMC7G3,VrZAG29,VMC5G7,and VVIB23)have been identified.Using these markers in HRM-PCR analysis,we assessed genetic diversity among a large collection of 192 grapevine varieties.The grapevine germplasm used encompasses the majority of Greek vineyard with 181 varieties,3 prominent foreign varieties and 11 varieties of Palestinian origin.The SSR markers used were highly polymorphic,displaying unique melting curves for unusually higher number of samples than generally observed in SSR analysis.This prompted us to examine sequence composition for selected samples and found that variation present as SNPs in the flanking sequences of SSR motifs was responsible for the observed polymorphism.Hence,HRM-PCR proved to be a tool of higher analytical power to distinguish genotypes surpassing the discrimination power of conventional gel-based SSR analysis.The study provides a better understanding of genetic variation of SSR marker loci associated to phenological traits in grapevine varieties,signifying an analytical methodology that may be of higher discrimination power in detection of polymorphism for utilization in breeding programs. 展开更多
关键词 vitis vinifera l phenological QTls microsatellites GENOTYPING High-resolution melting
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