目的:研究油茶肉质果提取物(Camellia oleifera Abel fleshy fruit extract,CFFE)的成分及不同浓度CFFE抗葡聚糖硫酸钠(DSS)诱导的结肠炎作用。方法:使用液相色谱质谱联用技术(LC-MS)对CFFE的成分进行分析。将C57BL/6小鼠随机分为4组,...目的:研究油茶肉质果提取物(Camellia oleifera Abel fleshy fruit extract,CFFE)的成分及不同浓度CFFE抗葡聚糖硫酸钠(DSS)诱导的结肠炎作用。方法:使用液相色谱质谱联用技术(LC-MS)对CFFE的成分进行分析。将C57BL/6小鼠随机分为4组,先给药后造模。给药期间CFFE分别按高、低浓度给药,造模期间除对照组其余均进行3%DSS饮水处理。每天记录小鼠体重,处死小鼠时测量结肠长度,收集结肠组织分别通过RT-qPCR和ELISA方法测定肠道促炎症细胞因子的表达情况,HE染色检测炎症细胞浸润情况,使用Western-blot方法检测IκBα、p-IκBα、p38、p-p38、Erk、p-Erk、Jnk和p-Jnk蛋白表达情况。结果:CFFE含有多种成分,其中包括茶多酚、山柰酚和槲皮素等抗炎症分子。与对照组比较,模型组体重有明显下降、结肠长度缩短。与模型组比较,高浓度CFFE处理降低了IL-6和TNF-α的表达及炎症细胞浸润程度,且能显著抑制结肠组织的p-Jnk表达,但未影响p-IκBα、p-p38和p-Erk的表达。结论:CFFE含有多种抗炎症成分且对DSS诱导的结肠炎有预防作用,可能是通过抑制Jnk的活化来实现的。展开更多
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions w...The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.展开更多
文摘目的:研究油茶肉质果提取物(Camellia oleifera Abel fleshy fruit extract,CFFE)的成分及不同浓度CFFE抗葡聚糖硫酸钠(DSS)诱导的结肠炎作用。方法:使用液相色谱质谱联用技术(LC-MS)对CFFE的成分进行分析。将C57BL/6小鼠随机分为4组,先给药后造模。给药期间CFFE分别按高、低浓度给药,造模期间除对照组其余均进行3%DSS饮水处理。每天记录小鼠体重,处死小鼠时测量结肠长度,收集结肠组织分别通过RT-qPCR和ELISA方法测定肠道促炎症细胞因子的表达情况,HE染色检测炎症细胞浸润情况,使用Western-blot方法检测IκBα、p-IκBα、p38、p-p38、Erk、p-Erk、Jnk和p-Jnk蛋白表达情况。结果:CFFE含有多种成分,其中包括茶多酚、山柰酚和槲皮素等抗炎症分子。与对照组比较,模型组体重有明显下降、结肠长度缩短。与模型组比较,高浓度CFFE处理降低了IL-6和TNF-α的表达及炎症细胞浸润程度,且能显著抑制结肠组织的p-Jnk表达,但未影响p-IκBα、p-p38和p-Erk的表达。结论:CFFE含有多种抗炎症成分且对DSS诱导的结肠炎有预防作用,可能是通过抑制Jnk的活化来实现的。
基金National Natural Science Foundation of China(31100479)
文摘The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.