The parallel-plate flow chamber (PPFC), of which the height is far smaller than its own length and width, is one of the main apparatus for the in vitro study of the mechanical behaviors of cultured cells at the bottom...The parallel-plate flow chamber (PPFC), of which the height is far smaller than its own length and width, is one of the main apparatus for the in vitro study of the mechanical behaviors of cultured cells at the bottom of PPFC undergoing shear stress. The PPFC of which the upper and lower plates are rectangular is usually used by research workers, and the flow field in this kind of PPFC (except for the regions near the entrance and exit) is uniform([1]), so only the effect the shear stress with one value has on cultured cells can be observed during each experiment. A kind of PPFC of which the upper and lower plates are not rectangular is proposed in this paper. The distributions of the velocities inside and the shear stresses at the bottom of the chamber are given by analyzing the flow field of the steady flow in the PPFC. The results show that the mechanical behaviors of cultured cells undergoing the shear stresses with various values may be simultaneously observed by the use of this kind of irrectangular PPFC. The theoretical and experimental results obtained by Ultrasonic Doppler Technique show good agreement.展开更多
Introduction Blood flow provides a mechanical condition for blood cells and vessels,especially for endothelial cells.It is important to understand the mechanical characteristics of
The adhesion of endothelial progenitor cells（EPCs） on endothelial cells（ECs） is one of the critical physiological processes for the regenesis of vascular vessels and the prevention of serious cardiovascular diseas...The adhesion of endothelial progenitor cells（EPCs） on endothelial cells（ECs） is one of the critical physiological processes for the regenesis of vascular vessels and the prevention of serious cardiovascular diseases.Here,the rolling and adhesion behavior of EPCs on ECs was studied numerically.A two-dimensional numerical model was developed based on the immersed boundary method for simulating the rolling and adhesion of cells in a channel flow.The binding force arising from the catch bond of a receptor and ligand pair was modeled with stochastic Monte Carlo method and Hookean spring model.The effect of tumor necrosis factor alpha（TNF-α） on the expression of the number of adhesion molecules in ECs was analyzed experimentally.A flow chamber system with CCD camera was set up to observe the top view of the rolling of EPCs on the substrate cultivated with ECs.Numerical results prove that the adhesion of EPC on ECs is closely related to membrane stiff-ness of the cell and shear rate of the flow.It also suggests that the adhesion force between EPC and EC by P-selectin glycoprotein ligand-1 only is not strong enough to bond the cell onto vessel walls unless contributions of other catch bond are considered.Experimental results demonstrate that TNF-α enhanced the expressions of VCAM,ICAM,P-selectin and E-selectin in ECs,which supports the numerical results that the rolling velocity of EPC on TNF-α treated EC substrate decreases obviously compared with its velocity on the untreated one.It is found that because the adhesion is affected by both the rolling velocity and the deformability of the cell,an optimal stiffness of EPC may exist at a given shear rate of flow for achieving maximum adhesion rates.展开更多
A water model and a high-speed video camera were utilized in the 300-t RH equipment to study the effect of steel flow patterns in a vacuum chamber on fast decarburization and a superior flow-pattern map was obtained d...A water model and a high-speed video camera were utilized in the 300-t RH equipment to study the effect of steel flow patterns in a vacuum chamber on fast decarburization and a superior flow-pattern map was obtained during the practical RH process. There are three flow patterns with different bubbling characteristics and steel surface states in the vacuum chamber： boiling pattern（BP）, transition pattern（TP）, and wave pattern（WP）. The effect of the liquid-steel level and the residence time of the steel in the chamber on flow patterns and decarburization reaction were investigated, respectively. The liquid-steel level significantly affected the flow-pattern transition from BP to WP, and the residence time and reaction area were crucial to evaluate the whole decarburization process rather than the circulation flow rate and mixing time. A superior flow-pattern map during the practical RH process showed that the steel flow pattern changed from BP to TP quickly, and then remained as TP until the end of decarburization.展开更多
Pure nitrogen gas was heated with direct current arc, at input powers from several hundred Watt to over 5 kW, and then injected through a nozzle into a chamber at 1 or 10 Pa pressure, with the purpose of accelerating ...Pure nitrogen gas was heated with direct current arc, at input powers from several hundred Watt to over 5 kW, and then injected through a nozzle into a chamber at 1 or 10 Pa pressure, with the purpose of accelerating the gas to very high speed around 7 km/s. Various structures of the arc generator and gas expansion nozzle were examined. Results show that bypass exhausting of the boundary layer before it enters the nozzle divergent section can greatly increase flow speed of the jet, thus it might be possible to use nitrogen as a working gas in high speed gas dynamic test facilities.展开更多
A newly designed pneumatic spring with two separate chambers is promoted and double-loop active control is introduced to overcome the following drawbacks of passive pneumatic isolation： ① The low frequency resonance...A newly designed pneumatic spring with two separate chambers is promoted and double-loop active control is introduced to overcome the following drawbacks of passive pneumatic isolation： ① The low frequency resonances introduced into the system; ② Conflict between lower isolation frequency and stiffness high enough to limit quasi-static stroke;③ Inconsistent isolation level with different force load. The design of two separate chambers is for the purpose of tuning support frequency and force independently and each chamber is controlled by a different valve. The inner one of double-loop structure is pressure control, and in order to obtain good performance, nonlinearities compensation and motion flow rate compensation （MFRC） are added besides the basic cascade compensation, and the influence of tube length is studied. The outer loop has two functions： one is to eliminate the resonance caused by isolation support and to broaden the isolation frequency band by payload velocity feedback and base velocity feed forward, and the other is to tune support force and support stiffness simultaneously and independently, which means the support force will have no effect on support stiffness. Theoretical analysis and experiment results show that the three drawbacks are overcome simultaneously.展开更多
The Parallel-Plate Flow Chamber （PPFC）, of which the height is far smaller than its own length and width, is one of the main apparatus for the in-vitro study of the mechanical behavior of cultured vascular Endotheli...The Parallel-Plate Flow Chamber （PPFC）, of which the height is far smaller than its own length and width, is one of the main apparatus for the in-vitro study of the mechanical behavior of cultured vascular Endothelical Cells （ECs） exposed to fluid shear stress. The steady flow in different kinds of PPFC has been extensively investigated, whereas, the pulsatile flow in the PPFC has received little attention. In consideration of the characteristics of geometrical size and pulsatile flow in the PPFC, the 3-D pulsatile flow was decomposed into a 2-D pulsatile flow in the vertical plane, and an incompressible plane potential flow in the horizontal plane. A simple method was then proposed to analyze the pulsatile flow in the PPFC with spatial shear stress gradient. On the basis of the method, the pulsatile fluid shear stresses in several reported PPFCs with spatial shear stress gradients were calculated. The results were theoretically meaningful for applying the PPFCs in-vitro, to simulate the pulsatile fluid shear stress environment, to which cultured ECs were exposed.展开更多
A flow chamber whose length and width are far larger than its own height was widely used to study cell's mechanical behavior in vitro , especially the cell's adhesion property. The paper analyzed the flow fiel...A flow chamber whose length and width are far larger than its own height was widely used to study cell's mechanical behavior in vitro , especially the cell's adhesion property. The paper analyzed the flow field of the flow chamber in detail, and gave the analytical expressions of the velocity, pressure and shear stress when the fluid flowed in and out the chamber through a small crevice. Then, full and clear discussion of flow field was made. Also the velocity field was measured by an Ultrasonic Doppler Velocity-metre. It was found that the experimental values coincided with the theoretical values. These results were important to study the cell's mechanical behavior in vitro .展开更多
文摘The parallel-plate flow chamber (PPFC), of which the height is far smaller than its own length and width, is one of the main apparatus for the in vitro study of the mechanical behaviors of cultured cells at the bottom of PPFC undergoing shear stress. The PPFC of which the upper and lower plates are rectangular is usually used by research workers, and the flow field in this kind of PPFC (except for the regions near the entrance and exit) is uniform([1]), so only the effect the shear stress with one value has on cultured cells can be observed during each experiment. A kind of PPFC of which the upper and lower plates are not rectangular is proposed in this paper. The distributions of the velocities inside and the shear stresses at the bottom of the chamber are given by analyzing the flow field of the steady flow in the PPFC. The results show that the mechanical behaviors of cultured cells undergoing the shear stresses with various values may be simultaneously observed by the use of this kind of irrectangular PPFC. The theoretical and experimental results obtained by Ultrasonic Doppler Technique show good agreement.
基金supported by grant from National Natural Science Foundation of China No10772127,30570450Program for New Century Excellent Talents in University NCET-06-0789Sichaun Youth Science and Technology Foundation 06ZQ026-009
文摘Introduction Blood flow provides a mechanical condition for blood cells and vessels,especially for endothelial cells.It is important to understand the mechanical characteristics of
基金supported by the National Natural Science Foundation of China (10732070, 11072155)Shanghai Pujiang Program (09PJ1405800)
文摘The adhesion of endothelial progenitor cells（EPCs） on endothelial cells（ECs） is one of the critical physiological processes for the regenesis of vascular vessels and the prevention of serious cardiovascular diseases.Here,the rolling and adhesion behavior of EPCs on ECs was studied numerically.A two-dimensional numerical model was developed based on the immersed boundary method for simulating the rolling and adhesion of cells in a channel flow.The binding force arising from the catch bond of a receptor and ligand pair was modeled with stochastic Monte Carlo method and Hookean spring model.The effect of tumor necrosis factor alpha（TNF-α） on the expression of the number of adhesion molecules in ECs was analyzed experimentally.A flow chamber system with CCD camera was set up to observe the top view of the rolling of EPCs on the substrate cultivated with ECs.Numerical results prove that the adhesion of EPC on ECs is closely related to membrane stiff-ness of the cell and shear rate of the flow.It also suggests that the adhesion force between EPC and EC by P-selectin glycoprotein ligand-1 only is not strong enough to bond the cell onto vessel walls unless contributions of other catch bond are considered.Experimental results demonstrate that TNF-α enhanced the expressions of VCAM,ICAM,P-selectin and E-selectin in ECs,which supports the numerical results that the rolling velocity of EPC on TNF-α treated EC substrate decreases obviously compared with its velocity on the untreated one.It is found that because the adhesion is affected by both the rolling velocity and the deformability of the cell,an optimal stiffness of EPC may exist at a given shear rate of flow for achieving maximum adhesion rates.
基金financially supported by the National Natural Science Foundation of China (No.51704203)the PhD Early Development Program of Taiyuan University of Science and Technology (Nos. 20152008, 20152013, and 20152018)+2 种基金Shanxi Province Science Foundation for Youths (No. 201601D202027)Key Project of Research and Development Plan of Shanxi Province (Nos. 201603D111004 and 201603D121010)NSFC-Shanxi Coal Based Low Carbon Joint Fund (No. U1510131)
文摘A water model and a high-speed video camera were utilized in the 300-t RH equipment to study the effect of steel flow patterns in a vacuum chamber on fast decarburization and a superior flow-pattern map was obtained during the practical RH process. There are three flow patterns with different bubbling characteristics and steel surface states in the vacuum chamber： boiling pattern（BP）, transition pattern（TP）, and wave pattern（WP）. The effect of the liquid-steel level and the residence time of the steel in the chamber on flow patterns and decarburization reaction were investigated, respectively. The liquid-steel level significantly affected the flow-pattern transition from BP to WP, and the residence time and reaction area were crucial to evaluate the whole decarburization process rather than the circulation flow rate and mixing time. A superior flow-pattern map during the practical RH process showed that the steel flow pattern changed from BP to TP quickly, and then remained as TP until the end of decarburization.
基金supported by the National Natural Science Foundation of China(Nos.11575273 and 11475239)
文摘Pure nitrogen gas was heated with direct current arc, at input powers from several hundred Watt to over 5 kW, and then injected through a nozzle into a chamber at 1 or 10 Pa pressure, with the purpose of accelerating the gas to very high speed around 7 km/s. Various structures of the arc generator and gas expansion nozzle were examined. Results show that bypass exhausting of the boundary layer before it enters the nozzle divergent section can greatly increase flow speed of the jet, thus it might be possible to use nitrogen as a working gas in high speed gas dynamic test facilities.
基金This project is supported by Commission of Science Technology and Industry for National Defense, China.
文摘A newly designed pneumatic spring with two separate chambers is promoted and double-loop active control is introduced to overcome the following drawbacks of passive pneumatic isolation： ① The low frequency resonances introduced into the system; ② Conflict between lower isolation frequency and stiffness high enough to limit quasi-static stroke;③ Inconsistent isolation level with different force load. The design of two separate chambers is for the purpose of tuning support frequency and force independently and each chamber is controlled by a different valve. The inner one of double-loop structure is pressure control, and in order to obtain good performance, nonlinearities compensation and motion flow rate compensation （MFRC） are added besides the basic cascade compensation, and the influence of tube length is studied. The outer loop has two functions： one is to eliminate the resonance caused by isolation support and to broaden the isolation frequency band by payload velocity feedback and base velocity feed forward, and the other is to tune support force and support stiffness simultaneously and independently, which means the support force will have no effect on support stiffness. Theoretical analysis and experiment results show that the three drawbacks are overcome simultaneously.
基金Project supported by the National Natural Science Foundation of China (Grant Nos. 10472027, 30670511).
文摘The Parallel-Plate Flow Chamber （PPFC）, of which the height is far smaller than its own length and width, is one of the main apparatus for the in-vitro study of the mechanical behavior of cultured vascular Endothelical Cells （ECs） exposed to fluid shear stress. The steady flow in different kinds of PPFC has been extensively investigated, whereas, the pulsatile flow in the PPFC has received little attention. In consideration of the characteristics of geometrical size and pulsatile flow in the PPFC, the 3-D pulsatile flow was decomposed into a 2-D pulsatile flow in the vertical plane, and an incompressible plane potential flow in the horizontal plane. A simple method was then proposed to analyze the pulsatile flow in the PPFC with spatial shear stress gradient. On the basis of the method, the pulsatile fluid shear stresses in several reported PPFCs with spatial shear stress gradients were calculated. The results were theoretically meaningful for applying the PPFCs in-vitro, to simulate the pulsatile fluid shear stress environment, to which cultured ECs were exposed.
文摘A flow chamber whose length and width are far larger than its own height was widely used to study cell's mechanical behavior in vitro , especially the cell's adhesion property. The paper analyzed the flow field of the flow chamber in detail, and gave the analytical expressions of the velocity, pressure and shear stress when the fluid flowed in and out the chamber through a small crevice. Then, full and clear discussion of flow field was made. Also the velocity field was measured by an Ultrasonic Doppler Velocity-metre. It was found that the experimental values coincided with the theoretical values. These results were important to study the cell's mechanical behavior in vitro .
