Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (...Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (FHCM).A microarray was fabricated to screen mutations in exons 3,5,7,and 8 in cTnI gene.Primers were designed for the PCR (polymerase chain reaction) to amplify the target DNA fragments from fresh blood samples.In order to simplify the PCR process,multiplex PCR technology was investigated in detail.The concentration of Mg^(2+) played an important role in multiplex PCR process,a properly low concentration of Mg^(2+) submitted a better speciality of PCR products.The speciality was also favored when the annealing temperature was reasonably enhanced and 64℃is the optimal annealing temperature for the multiplex PCR systems.When applying the fabricated gene-chip to detect the target fragments from PCR mixture,the signal intensity sequence is in accordance with that from theoretic estimate.展开更多
Total RNA in tulips was extracted by Trizol method.Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant.The corresponding sections were amplified by RT-PCR and the PCR pr...Total RNA in tulips was extracted by Trizol method.Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant.The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP.The probes of plant virus,18S rRNA gene and comparisons were designed and immobilized on chips.Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software.Tobacco rattle virus was detected from tulips which were imported from Holand.The accuracy and sensitivity of the plant virus gene chip were proved.展开更多
文摘Hypertrophic cardiomyopathy (HCM) is one of the diseases damaging people health most badly and some mutations of exons in cardiac troponin I (cTnI) gene are closely associated with family hypertrophic cardiomyopathy (FHCM).A microarray was fabricated to screen mutations in exons 3,5,7,and 8 in cTnI gene.Primers were designed for the PCR (polymerase chain reaction) to amplify the target DNA fragments from fresh blood samples.In order to simplify the PCR process,multiplex PCR technology was investigated in detail.The concentration of Mg^(2+) played an important role in multiplex PCR process,a properly low concentration of Mg^(2+) submitted a better speciality of PCR products.The speciality was also favored when the annealing temperature was reasonably enhanced and 64℃is the optimal annealing temperature for the multiplex PCR systems.When applying the fabricated gene-chip to detect the target fragments from PCR mixture,the signal intensity sequence is in accordance with that from theoretic estimate.
文摘Total RNA in tulips was extracted by Trizol method.Primers were designed according to the sequences of Tobacco rattle virus and 18S rRNA gene of plant.The corresponding sections were amplified by RT-PCR and the PCR products were labeled by Cy3-dCTP.The probes of plant virus,18S rRNA gene and comparisons were designed and immobilized on chips.Labeled PCR products were hybridized with the probes and the signals were scanned by scanner and analyzed by GenePix Pro 4.0 software.Tobacco rattle virus was detected from tulips which were imported from Holand.The accuracy and sensitivity of the plant virus gene chip were proved.
