Effects of Different Flue-curing Technologies on Microscopic Structure of Flue-cured Tobacco Leaves

[Objective] This study aimed to compare the effects of Chinese quality-improving fragrunce-increasing tobacco flue-curing technology, Chinese three- stage tobaeeo flue-curing technology and Zimbabwean tobaceo flue-euring technology on microscopie structure of flue-cured tobacco leaves to provide theoretical basis for tobacco flue-curing. [ Method ] Middle leaves of tobacco cultivar K326 were collected in Pengshui County of Chongqing City for flue-curing experiment using three flue-curing technologies. Leaf samples were collected regularly in the flue-curing process, to investigate the microscopic structure of flue-cured tobaceo leaves. [ Result] During three flue-curing processes, leaves, palisade tissues and sponge tissues shrank gradually. Three flue-euring processes exhibited significant differ- ences in the peak of tissue shrinkage ： microscopic structure of flue-cured tobacco leaves in Chinese three-stage tobacco flue-curing process shrank earliest, follower by Chinese quality-improving fragrance-increasing tobacco flue-curing process; flue-eured tobacco leaves in Zimbabwean tobacco flue-curing process presented the latest shrinkage. At 84 h post-curing, leaf thickness in three flue-curing processes showed a descend order of Chinese three-stage tobacco flue-curing process 〉 Chinese quality-improving fragrance-increasing tobacco flue-curing process 〉 Zimbabwean tobacco flue-curing process. Upper and lower epidermal cells in Zimb- abwean tobacco flue-curing process ruptured earlier than other two flue-curing processes; eventually, the majority of cells ruptured and mixed with palisade tissues and sponge tissues. In Chinese quality-improving fragranee-increasing tobacco flue-curing process, only a small number of epidermal cells in dried leaves ruptured. Among three flue-curing technologies, Zimbabwean tobacco flue-curing technology exhibited the greatest damage to epidermal cells, followed by Chinese three-stage tobaceo flue-curing technology; Chinese quality-improving fragrance-increasing tobacco flue-curing technology had the minimum damage to epidermal cells. Mese- phyll cross-section exhibited significant morphological changes in stomata. To be specific, at 0 - 12 h post-curing, stomata of tobacco leaves closed with slightly prominent stamatal apparatuses on upper and lower epidermis; at 24 -72 h post-curing, stomata of tobaceo leaves changed gradually from opening to closure with significantly prominent stomatal apparatuses on upper and lower epidermis; at 84 h post-curing, stomata of tobacco leaves closed, and the majority of stomatal appa- ratuses were significantly prominent. In Chinese quality-improving fragrance-increasing tobacco flue-curing process, only a small number of epidermal ceils ruptured at 84 h pest-flue-curing; palisade tissues and sponge tissues shrank almost simultaneously. Significant gaps were observed between palisade tissues and between pal- isade tissues and sponge tissues. Chinese quality-improving fragrance-increasing tobacco flue-curing technology exhibited lower disorder level compared with other two flue-curing technologies. [ Conclusion] Chinese quality-improving fragrance-increasing tobacco flue-curlng technology was conducive to maintaining the micro- scopic structure integrity of flue-cured tobacco leaves and obtaining high-quality flue-cured tobacco leaves.

Effects of Different Flue-curing Technologies on Submicroscopic Structure of Flue-cured Tobacco Leaves

[ Objective] This study aimed to compare the effects of low-temperature low-humidity tobacco flue-curing technology, moderate-temperature moderate- humidity tobacco flue-curing technology and moderate-temperature high-humidity tobacco flue-curing technology on submicroscopic structure of flue-cured tobacco leaves to provide theoretical basis for tobacco flue-curing. [ Method] Middle leaves of tobacco cuhivar K326 were collected in Pengshui County of Chongqing City for flue-curing experiment using three flue-curing technologies. Leaf samples were collected regularly in the flue-curing process, to investigate the submicroscopic structure of flue-cured tobacco leaves. [ Result] Morphology changes and structural rupture of chloroplasts occurred at different time during three flue-curing proces- ses. In low-temperature low-humidity flue-curing process, morphology changes and structural rupture of chloroplasts occurred late but rapidly; in moderate-tempera- ture moderate-humidity flue-curing process and moderate-temperature high-humidity flue-curing process, morphology changes and structural rupture of chloroplasts occurred early but slowly. Among these three flue-curing processes, the speed of changes in mitochondria morphology, cristae number and membrane rupture pres- ented an upward order of low-temperature low-humidity flue-curing process 〈 moderate-temperature high-humidity flue-curlng process 〈 moderate-temperature moderate-humidity flue-curing process. In low-temperature low-humldity flue-curing process, starch granules in chloroplasts ruptured after flue-curing for 36 -48 h ; in moderate-temperature moderate-humidity flue-curing process, starch granules in chloroplasts ruptured after flue-curing for 24 - 36 h; in moderate-temperature high-humidity flue-curing process, starch granules in chloroplasts ruptured after flue-curing for 12 - 24 h. [ Conclusion ] Using low-temperature low-humidity flue- curing technology could prolong the maintenance duration of the submicroscopic structure integrity of flue-cured tobacco leaves, which was conducive to fully decom- posing and transforming substances in tobacco leaves and obtaining high-quality flue-cured tobacco leaves.

彰显龙岩CB-1烟叶清甜蜜甜香型风格密集烘烤工艺研究

研究龙岩地区‘翠碧一号’的密集烘烤工艺,为彰显其清甜蜜甜香型风格提供参考。为彰显‘翠碧一号’烟叶清甜蜜甜香型风格,以福建龙岩烟区栽种‘翠碧一号’下、中、上部的适熟烟叶为实验材料,设置3种烘烤工艺,采用“背靠背”编烟方式编竿装烟,对烤后烟叶分别按下、中、上部进行分级,然后进行鉴定,统计外观、化学成分及感官质量等信息。结果显示,‘翠碧一号’(CB-1)品种烟叶下部烟宜采用T3(高温/高低高湿)烘烤工艺,关键烘烤阶段干/湿球温度为:变黄期38~40/37~38℃、凋萎期42/36℃、变筋期46/36℃、干片期50~54/38~39℃、干筋期60~65/40~42℃,中、上部烟叶宜采用T1(高温/高湿)烘烤工艺,关键烘烤阶段干/湿球温度为:变黄期38~40/37~38℃、凋萎期42/38℃、变筋期46/38℃、干片期50~54/39~40℃、干筋期60~65/40~42℃。该研究为彰显‘翠碧一号’香型的密集烘烤工艺提供技术参考。

Yield and Nicotine Content of Flue-Cured Tobacco as Affected by Soil Nitrogen Mineralization

Nitrogen(N)supply is the most important factor affecting yield and quality of flue-cured tobacco(FCT).A field experiment and an in situ incubation method were used to study the effects of soil N mineralization in the later stages of growth on yield and nicotine content of FCT in Fenggang and Jinsha,Guizhou Province.The yield and market value of FCT at Fenggang were much lower than those at Jinsha.However,the nicotine content of middle and upper leaves was much higher at Fenggang than at Jinsha when the same rate of fertilizer N was applied,which might be due to a higher N supply capacity at the Fenggang site.At later stages of growth(7-16 weeks after transplanting),the soil net N mineralization at Fenggang(56 kg N ha^(-1))was almost double that at Jinsha(30 kg N ha^(-1)).While soil NH_4-N and NO_3-N were almost exhausted by the plants or leached 5 weeks after transplanting,the N taken up at the later growth stages at Fenggang were mainly derived from soil N mineralization,which contributed to a high nicotine content in the upper leaves.The order of soil N contribution to N buildup in different leaves was:upper leaves>middle leaves>lower leaves.Thus,soil N mineralization at late growth stages was an important factor affecting N accumulation and therefore the nicotine content in the upper leaves.

Studies on Amylase and Degradation of Starch and Pigment of Tobacco Leaf During Process of Flue-Curing

The changes in the activity of amylase and amylase-isoenzyme and the degradation of starch and pigment of tobacco leaf during flue-curing were studied by using the electric- heated flue-curing barn designed and made by the Henan Agricultural University. The temperature and humidity of the barn were controlled automatically. The results indicated that starch in tobacco leaf decreased rapidly and leveled off after 48 h of curring, in the meantime, the content of soluble sugar increased accordingly and reached a peak at the stage of color-fixing. Both of them had a rapid-changing stage in the first 36 hours of yellowing. The changes of starch and soluble sugar contents had highly significant negative-correlation at 1 % level (rNC89 = -0.8962**, rYY85 = -0.9704**). The activity of amylase increased with the proceeding of curing and reached a peak after 36 hours of curing, then decreased. But the activity of amylase kept at a high level when the humidity of curing-environment was very low, even if the tobacco leaf had been dried. The rapid degradation of starch showed a significantly negative correlation with the increase of activity of amylase at 5 % level (rNC89 = -0.8495*, rYY85 = -0.7839*). The degradation of starch and pigment had the same regulation and had highly significant correlation at 1 % level (rNC89= 0.9649**, rYY85= 0.9428**). There were mainly three amylase-isoenzyme bands -A, B, C respectively, in tobacco leaf during flue curing. They were identified as α-AMY, β-AMY, R-AMY, and the activity of β-AMY was the highest. The changes in amylase activity and contents of starch and pigment were affected by the tobacco leaf moisture and environmental humidity during curing.

Comparative Analysis on Chemical Components and Sensory Quality of Aging Flue-Cured Tobacco from Four Main Tobacco Areas of China

Complicated changes are happening during the aging process of flue-cured tobacco （FCT） and understanding of the changing components is of great significance in improving the quality,shortening aging time,and enhancing production and economic efficiency in tobacco industry.The aging samples of FCT from four main producing areas of China,i.e.,Yunnan,Sichuan,Hubei,and Dongbei,were used to study the changing rule of the components such as alkali,acid,and carbohydrate as well as the aroma and their relationship with sensory quality;and based on the correlation among the components and the sensory quality index,multivariable models were established to predict the aging quality.The results showed that the sensory quality of FCT increased steadily during the aging time,and at the same time,the acidic components （total organic acids,volatile acids） increased gradually,while the alkaline substances （nicotine,volatile alkali）,carbohydrate （total sugars,reducing sugar） and pH values showed a downward trend.Correlation analysis found that the sensory quality and pH values were negatively correlated （P0.05）,while the sensory quality with total organic acids and aroma were positively correlated.The optimal model for predicting the quality of FCT was y=56.75-0.63x12＋50.09x2-13.27x22,（y：sensory quality;x1：pH;x2：total organic acids）.The average predicating error was 1.04% with maximum predicating error of 2.89% and the accuracy rate of above 97%.

YCB-Ⅲ对烤烟农艺性状和病毒病防治的影响

采用土壤改良剂(YCB-Ⅲ)与农药配施,在减施农药总用量的同时不降低(或提升)对烤烟病毒病的防治效果,且改善烤烟的农艺性状,提高烤烟的产量与品质。从不同处理对烤烟旺长期农艺性状的影响看,T4和T5对烤烟的株高、茎围、有效叶片数、鲜叶重和开片度均达到显著水平。相对于T1,T2、T3、T4和T5的YCB-Ⅲ施用量分别增加了25%、50%、75%和100%,农药施用量分别减少了0.00%、25%、50%和50%,但是防治效果均大于74%,处于同一水平。研究表明,YCB-Ⅲ的施用量525 kg/hm~2、4%嘧肽霉素水剂(KV_1)有效成分施用量120 g/hm~2、0.5%香菇多糖水剂(KV_2)有效成分施用量12.5 g/hm~2为最佳推荐量。

Proteomics Identification of Differentially Expressed Proteins Relevant for Nicotine Synthesis in Flue-Cured Tobacco Roots Before and After Decapitation

Nicotine is a secondary substance synthesized in tobacco roots. In flue-cured tobacco planting, tobacco decapitation is an effective practice to promote nicotine biosynthesis by regulation of the redistribution of total nitrogen amounts. However, proteins relevant to nicotine synthesis in tobacco roots has not been identified and characterized yet. It is important to explore the regulation of nicotine biosynthesis in tobacco roots. To identify the proteins relevant to nicotine synthesis, the protein patterns in roots of flue-cured tobacco （cv. K326） before and after decapitation were analyzed. In the present study, the protein patterns in roots of flue-cured tobacco were analyzed by two-dimensional electrophoresis （2-DE）, and the differentially-expressed spots were identified by matrix assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS）. Paired comparison of 2-DE maps revealed 26 spots of differentially-expressed proteins in roots before and after decapitation. Furthermore, nine differentially-expressed spots were identified. There were four proteins which were enzymes possibly involved in nicotine biosynthesis. In addition, the roles of the four enzymes in nicotine biosynthesis were discussed in a putative network. Our results would contribute to the understanding of the regulation pathway of nicotine biosynthesis and further to the molecular manipulation on the nicotine contents in flue-cured tobacco.

基于SIDA-GC-QqQ-MS/MS的烤烟中痕量香气化合物2-乙酰基-1-吡咯啉的测定

【目的】精确测定烤烟中痕量香气化合物2-乙酰基-1-吡咯啉(2AP)的含量。【方法】采用稳定同位素稀释结合气相色谱-串联三重四级杆质谱仪MRM多反应监测模式对烤烟中2-乙酰基-1-吡咯啉进行精确定量分析。【结果】(1)方法在2~200μg/kg范围内线性良好(R^(2)=0.9998),检测限为0.38μg/kg,定量限为1.26μg/kg,平均回收率为90.75%~96.10%。(2)烤烟烟叶中2AP含量为2.02~62.48μg/kg,主流烟气中含量为7.29~94.12μg/kg,其中粒相物和气相物的含量范围分别为2.08~79.08μg/kg和5.15~18.68μg/kg。【结论】方法稳定可行、高效简便,具有较高的精度和灵敏度,可为烟用香精香料配方设计开发提供数据支撑,为烤烟中其他痕量香气化合物的精确定量分析研究提供方法参考。

Characteristics of Potassium-Enriched, Flue-Cured Tobacco Genotype in Potassium Absorption, Accumulation, and In-Ward Potassium Currents of Root Cortex

This study was to investigate the main traits of potassium-enriched, flue-cured tobacco genotypes related to potassium absorption, accumulation, and in-ward potassium currents of the root cortex. Hydroponic methods, K^＋-depletion methods, and patch-clamp, whole-cell recordings were conducted to study the accumulation of dry matter and potassium in different organs, and to measure potassium absorption and dynamic and in-ward potassium currents in potassium-enriched, fluecured tobacco genotypes. The average dry weights of leaves and whole plant of potassium-enriched, flue-cured tobacco genotype ND202 were 10.20, and 14.85 g, respectively, higher than JYH （8.50 and 13.11 g, respectively） and NC2326 （8.39 and 12.72 g, respectively）, when potassium concentration in the solution ranged from 0.1 to 50 mmol L^-1. Potassium accumulation in the leaves of ND202 was 18.6% higher than JYH and 34% higher than NC2326 when potassium concentration in the solution was superior to 0.5 mmol L^-1. The Vmax （the maximum velocity） of ND202 was 118.11 lamol FW g^-1 h^-1, obviously higher than that of JYH （58.87 μmol FW g^-1 h^-1） and NC2326 （64.40 μmol FW g^-1 h^-1）. In the in-ward potassium currents, the absolute value of current density （pA/pF） of ND202 was 60, higher than that of JYH （50） and NC2326 （40）. Potassium concentration in leaves, Vmax, and in-ward potassium currents, could be used to screen potassium-enriched, flue-cured tobacco genotypes.

Relationship Between Hyperspectral Parameters and Physiological and Biochemical Indexes of Flue-Cured Tobacco Leaves

The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents （total nitrogen, chlorophyll, carotenoid）, and physiological indexes （fresh weight, dry weight, moisture content） of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.

基于VGG16-DenseNet集成模型的烤烟智能分级

为实现烤烟烟叶等级快速、准确的智能化识别,本研究基于手机拍摄的不同品种烤烟烟叶正、反面图像,构建了VGG16与DenseNet组合的新网络模型VGG16-Dense,并应用手机拍摄的翠碧1号、云烟87烤烟烟叶6个等级正反面图片,总共24类,验证该模型的有效性,同时与5个网络模型DenseNet121、ResNet50、AlexNet、VGG16和GoogLeNet进行比较。研究表明:VGG16-Dense网络模型在验证集的各评估指标(准确率、精确率、召回率、F1分数和平均损失值)均达到优秀值,在测试集的各评估指标较其他模型是最优的,准确率为92.71%,精确率为93.07%,召回率为92.71%,F1分数为92.72%,平均损失值为0.22,有较好的泛化能力,错判较少。VGG16-Dense网络模型能同时智能判别烤烟烟叶等级及其正反面,甄别不同品种,这为初级烤烟收购中的定级实现智能化提供理论指导。

Changes of Polyphenols in Tobacco Leaves During the Flue-Curing Process and Correlation Analysis on Some Chemical Components

The changes of polyphenols in tobacco leaves during the flue-curing process and correlation analysis on some chemical components were studied. Leaf samples were taken from different tobacco-producing regions in Henan Province, China. The results indicated that the content of total phenols increased during the first 24 h of curing, and then decreased. It reached the lowest value at 72 h of curing and increased rapidly after that. The content of chlorogenic acid also increased during 0-24 h of curing. But the lowest point occurred at 60 or 72 h of curing and then it increased till the end of the curing process. The content of mtin generally increased with curing, and showed little fluctuations. The changes of PPO and POD activity were the opposite. Rutin was found to have a highly significant positive correlation with total sugar （r=0.822^＊＊）, but a highly significant negative correlation with starch, nicotine, and protein.

The Quality Difference of Tobacco Leaves Between Different Flue-curing Processes in Honghe Tobacco-growing Area

[Objectives] This study was conducted to compare the effects of different curing processes,in order to optimize the local supporting intensive fluecuring process.[Methods] A comparative experiment was carried out in Mile,Luxi and Jianshui on the four-layer tobacco-packed intensive curing process(K1)and the eight-point precise and intensive curing process(K2).[Results]The results showed that according to the appearance evaluation of the tobacco leaves,K1 was higher than K2 in respective scores of color,maturity,leaf structure and defects and the total score,while K1 equaled K2 in the scores of identity,oil content,chroma and length.The differences in respective scores of the two processes were not significant.According to the results of sensory evaluation,the two tobacco enterprises got different evaluation results of unblended cigarettes made of the middle leaves due to different raw material requirements of the enterprises by different processes.K1 was higher than K2 in the sensory evaluation of China Tobacco Yunnan Industrial Co.,Ltd.,while it was lower than K2 in that of China Tobacco Zhejiang Industrial Co.,Ltd.,and the difference in the total score between the two processes was significant.From the content of chemical components,in the conventional chemical component contents including total sugar,reducing sugar,chlorine and starch,K1 was higher than K2,while in the contents of potassium,total phytonine,total nitrogen and petroleum ether extract,K1 was lower than K2,but the differences were not significant.K1 was lower than K2 in the contents of neutral aroma components including ketones,alcohols,aldehydes,heterocyclics,neophytadiene and total content,while K1 was higher than K2 in the contents of esters,phenols,but the respective differences were not significant.Except the significant difference in the total score in the sensory evaluation of China Tobacco Zhejiang Industrial Co.,Ltd.,there were no significant differences in other items in the appearance evaluation,sensory evaluation and chemical composition of tobacco leaves between K1 and K2.[Conclusions] The supporting intensive curing process can be further optimized on the basis of this study.

烤烟-粮经作物间套作模式及在重庆的应用

间套作种植模式作为中国农业传统遗产,既可提高质量,又能增加经济收入,在我国粮食增长与农业增收中一直发挥着重要作用.仓禀实而民心安,粮食安全是“国之大者”;而烟草是我国重要的经济作物,是国家税收的重要来源.在国家强化粮食安全背景下,烟粮争地问题愈来愈突出.在烟区发展烤烟-粮经作物间套作种植模式,形成以烟稳粮、以烟促粮的良好局面,具有重要意义.本文综述了烤烟间套作生理生态效应、套作对烟叶产质量及病害的影响、烟草适宜的间套作作物、烤烟套作存在的问题及其解决措施等,并对重庆市第一烤烟种植大县彭水县发展烤烟间套作种植模式进行了展望.

Risk Regionalization of Drought for Flue-cured Tobacco Planting in Qujing City Based on GIS

The risk regionalization of drought for flue-cured tobacco planting in Qujing City was studied to provide reference for drawing on advantages and avoiding disadvantages in flue-cured tobacco planting,disaster reduction,and disaster relief services.According to the production practice of flue-cured tobacco and local climate analysis,the risk of disaster-causing factors,sensitivity of disaster-breeding environment,vulnerability of carriers,and disaster prevention and reduction capability were analyzed and evaluated quantitatively.Secondly,starting from the formation mechanism of drought,a risk assessment model of the meteorological disaster was established by GIS technology.Finally,the risk assessment and regionalization of drought in the critical periods for drought-stricken flue-cured tobacco (transplanting period,root extending stage and vigorous growth period) in Qujing City were carried out.The results showed that in Qujing City,the risk areas of drought for flue-cured tobacco planting were divided into five grades in the transplanting period and six grades in the root extending stage and vigorous growth period.

Effect of Different Intercropping Methods on the Reduction of Heavy Metal Content in Flue-Cured Tobacco

Heavy metals not only adversely affect the quality of tobacco leaves,but also have very adverse effects on human health. In order to find out the effective cultivation method of controlling heavy metal content in flue-cured tobacco,the content of four kinds of heavy metals in soil and tobacco leaves in different intercropping treatments was tested. The results showed that there were significant differences between the effects of intercropping on the content of some heavy metals in soil and tobacco leaves. Different interplanting methods had a positive effect on the reduction of heavy metal content in tobacco leaves and play a very important and positive role in actual production.

Effects of Amount of Green Manure Returned to Field on Yield and Quality of Flue-cured Tobacco

The effects of amount of green manure returned to field on yield and quality of flue-cured tobacco were studied by field experiment. The results showed that significant positive correlation existed between tobacco leaf yield and small or moderate amount of green manure returned to field. Path analysis showed that moderate amount of green manure returned to field affected yield and quality of tobacco leaves, small green manure returned to field mainly affected leaf yield, while large amount of green manure returned to field mainly affected leaf quality. Therefore, the effect of moderate amount of green manure returned to field on yield and quality of tobacco leaves was best, and moderate amount of green manure returned to field was recommended in production of flue-cured tobacco.

烤烟专用有机-无机复混肥对烟草生长的影响

【目的】为探究烤烟专用有机-无机复混肥对烤烟K326生长的影响。【方法】在广西百色植烟区采用田间试验方法,以K326为试验材料,设置3个不同施肥量处理,以当地常规施肥处理为对照。在追肥时间和次数一致的情况下,研究常规施肥和烤烟专用有机-无机复混肥对K326产量和质量的影响。【结果】以施用1800 kg/m2烤烟专用有机-无机复混肥处理效果最佳,其株高、茎围和上部叶叶长和叶宽分别较CK组增加了4.22%、3.00%、4.85%和2.25%,促生效果显著;烟株抵御病害能力增强,常见病害发生率降低29.26%~52.53%,产量和产值分别较常规对照增加8.96%和13.41%,经济效益显著;同时烟叶化学成分协调性和物理特性均在适宜范围内,且香气质较好,杂气和刺激性明显减少,余味舒适,浓度适中。【结论】施用1800 kg/m2烤烟专用有机-无机复混肥,能有效改善K326烟株农艺性状,降低病害发生率,增加经济效益,同时烟叶化学成分协调性、物理特性和感官质量均有所提升,可在广西K326种植区推广使用。

基于随机蛙跳筛选的初烤烟叶中β-胡萝卜素和叶黄素含量近红外模型的建立

为提高近红外光谱法测定初烤烟叶中β-胡萝卜素和叶黄素含量的准确性和稳定性,采用随机蛙跳(Random Frog,RF)筛选特征波长建立上述指标的偏最小二乘(Partial Least Squares,PLS)近红外模型,结合模型内部交互验证结果确定最优建模波长数和主成分数,并对最优模型的准确性和稳定性进行内外部评价。结果表明:相较于全波长(1298个波长)建模,利用RF筛选出的181个和216个特征波长建模,模型的决定系数(R2)由0.7718和0.8677分别提高至0.9511和0.9587,校正均方根误差(RMSEC)由3.362μg/g和4.930μg/g分别降低至1.556μg/g和2.756μg/g,交互验证均方根误差(RMSECV)由3.304μg/g和4.836μg/g分别降低至1.529μg/g和2.703μg/g;模型外部预测集(样品数=50)的预测平均相对误差分别由11.80%和9.62%降低至5.48%和5.49%。采用RF筛选特征波长能有效提升初烤烟叶β-胡萝卜素和叶黄素含量近红外模型的准确性和稳定性,可为初烤烟叶质量评价提供理论参考。