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Obtaining the Fluorescent Chitosan for Investigations in the Analytical Ultracentrifuge
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作者 Murodkhon R. Kodirkhonov 《Advances in Biological Chemistry》 2019年第1期23-30,共8页
1) In order to achieve the visibility of the chitosan macromolecule for the UV optical system of the analytical ultracentrifuge on investigation of the molecular characteristics and polymers interactions, the labeling... 1) In order to achieve the visibility of the chitosan macromolecule for the UV optical system of the analytical ultracentrifuge on investigation of the molecular characteristics and polymers interactions, the labeling of chitosan by a new fluorophore of fluorescein-5-isothiocyanat was carried out. 2) Samples of fluorescent chitosan with two different degrees of fluorophore substitution and various degrees of acetylation were obtained. 3) The labeled chitosans with the fluorescein-5-isothiocyanat allowed estimating the sedimentation coefficient and the molecular characteristic in the analytical ultracentrifuge. 4) The sensitivity of the UV-optical system of the analytical ultracentrifuge for the obtained fluorescent samples of chitosan relatively to the fixation of the meniscus and the influence of the wavelength and rotation speed were estimated. 展开更多
关键词 POLYSACCHARIDE CHITOSAN ANALYTICAL Ultracentrifuge fluorescein-5-isothiocyanat FLUORESCENT CHITOSAN Labeling Sedimentation Degree of Substitution UV Absorption
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