Coastal wetlands are hotspots for nitrogen(N)cycling,and crab burrowing is known to transform N in intertidal marsh soils.However,the underlying mechanisms remain unclear.This study conducted field experiments and use...Coastal wetlands are hotspots for nitrogen(N)cycling,and crab burrowing is known to transform N in intertidal marsh soils.However,the underlying mechanisms remain unclear.This study conducted field experiments and used indoor control test devices to investigate the seasonal response of nitrogen to crab disturbance at the sediment-water interface in coastal tidal flat wetlands.The results showed that crab disturbance exhibited significant seasonality with large seasonal differences in cave density and depth.Due to crab disturbance,nitrogen fuxes at the sediment-water interface were much greater in the box with crabs than in the box without crabs.In summer,NH-N showed a positive flux from the sediment to the overlying water,but NO2-N and NOg-N showed positive fluxes from the sediment to the overlying water only in early stages.In winter,NH-N showed a positive flux from the sediment to the overlying water,but NO-N and NO,-N both exhibited positive and negative fluxes.These results indicated that the presence of crab burrows can cause the aerobic layer to move downward by approximately 8-15 cm in summer and directly promote nitrification at the sediment surface.展开更多
Hydroxyapatite(HA)is a bio ceramic commonly utilized in bone tissue engineering due to its bioactive and osteoconductive properties.Crab shells are usually disregarded as waste material despite their significant CaCO_...Hydroxyapatite(HA)is a bio ceramic commonly utilized in bone tissue engineering due to its bioactive and osteoconductive properties.Crab shells are usually disregarded as waste material despite their significant CaCO_(3) content,and have not been widely utilized in the synthesis of HA.This study aims to synthesize and analyze HA derived from crab shells using the hydrothermal method with different durations of holding time.This study utilized precipitated calcium carbonate(PCC)derived from crab shells.With a hydrothermal reactor set at 160℃ and varying holding times of 14(HA_14),16(HA_16),and 18(HA_18)h,a PCC and(NH4)2HPO4 mixture was used to synthesize HA.The synthesis results were analyzed using scanning electron microscopy(SEM),fourier transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD)tests.This study has accomplished the synthesis of HA from crab shells.Nonetheless,the final product of synthesis still contained CaCO_(3) as an impurity.The prolonged hydrothermal holding time of 14 to 18 h resulted in a reduction of impurities while increasing the percentage of crystal weight and crystallite size of HA.Specimen CH_18 is the best-quality product generated in this study.This specimen produced HA with the highest percentage of crystal weight and crystallite size compared to the other specimens.Furthermore,specimen CH_18 exhibited the lowest concentration of impurities.The Ca/P ratio in this specimen was also the closest to 1.67.The Ca/P ratio,crystallite size,and crystal weight percentage of this specimen are 1.54,19.06 nm,and 99.1%,respectively.展开更多
In the two-dimensional positioning method of pulsars, the grid method is used to provide non-sensitive direction and positional estimates. However, the grid method has a high computational load and low accuracy due to...In the two-dimensional positioning method of pulsars, the grid method is used to provide non-sensitive direction and positional estimates. However, the grid method has a high computational load and low accuracy due to the interval of the grid. To improve estimation accuracy and reduce the computational load, we propose a fast twodimensional positioning method for the crab pulsar based on multiple optimization algorithms(FTPCO). The FTPCO uses the Levenberg–Marquardt(LM) algorithm, three-point orientation(TPO) method, particle swarm optimization(PSO) and Newton–Raphson-based optimizer(NRBO) to substitute the grid method. First, to avoid the influence of the non-sensitive direction on positioning, we take an orbital error and the distortion of the pulsar profile as optimization objectives and combine the grid method with the LM algorithm or PSO to search for the non-sensitive direction. Then, on the sensitive plane perpendicular to the non-sensitive direction, the TPO method is proposed to fast search the sensitive direction and sub-sensitive direction. Finally, the NRBO is employed on the sensitive and sub-sensitive directions to achieve two-dimensional positioning of the Crab pulsar. The simulation results show that the computational load of the FTPCO is reduced by 89.4% and the positioning accuracy of the FTPCO is improved by approximately 38% compared with the grid method. The FTPCO has the advantage of high real-time accuracy and does not fall into the local optimum.展开更多
The science of radio astronomy focuses on the observation and study of celestial objects by reading their radio waves. The 5 meter radio-telescope is able to observe different radio sources using a C-band LNB. This re...The science of radio astronomy focuses on the observation and study of celestial objects by reading their radio waves. The 5 meter radio-telescope is able to observe different radio sources using a C-band LNB. This research was essentially focused on Crab Nebula, also known as Taurus A. The study led to interesting observations, which were validated numerically using various scientific computing software. The radio waves emitted by Taurus A are readable by the RTL-SDR, a software defined radio receiver. This device is capable of reading radio frequencies in the range of 0.5 MHZ to 1700 MHZ.展开更多
The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.The...The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.展开更多
对于脉冲星试验01星的两年实测数据,针对蟹状星云脉冲星星历经历了多次更新的特点,提出了更新标准脉冲轮廓进行X射线计时的改进方法。以一系列观测时长为实测数据分组,再采用改进后的方法进行计时分析并与改进前的结果进行对比。通过对...对于脉冲星试验01星的两年实测数据,针对蟹状星云脉冲星星历经历了多次更新的特点,提出了更新标准脉冲轮廓进行X射线计时的改进方法。以一系列观测时长为实测数据分组,再采用改进后的方法进行计时分析并与改进前的结果进行对比。通过对比改进前后的计时结果,发现改进方法得到的计时残差的均方根(root mean square, RMS)平均降低了14.2%,定位精度平均提高了14.4%,估计原子钟相对频率偏差的精度平均提高了26.2%,体现了改进方法处理经历多次星历更新的Crab脉冲星观测数据的合理性和优越性。此外,改进方法对脉冲星试验01星两年观测数据的稳定度σ_(z)没有量级上的改进,说明改进方法对Crab脉冲星时间稳定度的影响不大。展开更多
The aim of the present study was to determine the MICs using broth microdilution method and their combination effect by (minimum inhibitory concentrations) of selected antibiotic agents determination of FICindex (f...The aim of the present study was to determine the MICs using broth microdilution method and their combination effect by (minimum inhibitory concentrations) of selected antibiotic agents determination of FICindex (fractional inhibitory concentrations). Lysozyme Anodonta cygnea was more active than Lysozyme Uniopictorum, especially against Gram-negative bacterias. Combination of Macropen and Lysozyme Unio pictorum has a synergistic effect, while a separation of these antibacterial agents was not active against some of the strains of microorganisms.展开更多
A diffraction-limited storage ring with a multi-bend achromat lattice suffers from a small dynamic aperture for conventional off-axis injection.Thus,a longitudinal on-axis injection scheme based on a new type of crab ...A diffraction-limited storage ring with a multi-bend achromat lattice suffers from a small dynamic aperture for conventional off-axis injection.Thus,a longitudinal on-axis injection scheme based on a new type of crab cavity is proposed in this paper.Particle tracking simulations were performed to study the disturbance of the stored beam and the motion of the injected beam during the injection process.The possibility of multi-bunch injections was discussed.In addition,the effect of the long-range wake field induced by the stored beam was analyzed.A C-band standing-wave crab cavity was designed and produced as requested,and its field distribution was measured.The corresponding results are consistent with the simulation results.展开更多
Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of crabs.However,no continuous crab cell line has been available due to the...Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of crabs.However,no continuous crab cell line has been available due to the lacking of suitable medium and the occurrence of mitosis-arrest.In this study,long-term in vitro culture conditions for both two-(2D)and three-dimensions(3D)were successfully developed for the circulating hemocytes of swimming crab Portunus trituberculatus,designated as PTH cells.In 2D culture,a novel crab basic medium in osmolarity of 990–1100 mOsm/kg was optimized for the first time,which is different from Leibovitz's L-15 medium in mainly the components of amino acids,containing double strengths of the contents of free amino acid mixture in the crab serum.Then an optimal crab growth medium was developed by supplementing 5%fetal bovine serum,50-g/L yeast extract powder,20-μg/L basic fibroblast growth factor and epidermal growth factor into the optimal crab basic medium,and found that it could support a long-term survival of PTH cells in a healthy monolayer up to 347 days and partially break through the mitosis-arrest of crab cells evidenced by the obvious increase of proliferating potential detected in the 10-d primarily cultured PTH cells.These 2D cultured PTH cells could be successfully sub-cultured for 11 times by physical flushing method and well cryopreserved in liquid nitrogen.In 3D culture,using the same crab growth medium,the PTH cell aggregates could be easily formed and healthily maintained on the surface of solidified Matrigel or in the ultra-low-attachment plate with a survival rate of 50%–60%on Day 103.This work largely improved the primary culture and subculture of crab cells and will facilitate the establishment of continuous crab cell line.展开更多
Coastal agriculture is vulnerable to climate change, thereby may affect food production systems and food security in Bangladesh. Methane (CH4) emission from coastal wetlands rice farming is a major environmental conce...Coastal agriculture is vulnerable to climate change, thereby may affect food production systems and food security in Bangladesh. Methane (CH4) emission from coastal wetlands rice farming is a major environmental concern due to its global warming potential. Therefore, field experiments were conducted at the southern coastal region of Shyamnagar, Satkhira, to investigate the feasibility of Rice-Shrimp and Rice-Crabs mixed farming for adaptation to the changing climate and sustaining food production system. The experimental treatments were designed on rice-based diversified farming systems such as rice sole cropping with no NPKS + no soil amendments (T1), rice sole cropping following farmers’ practice (FP) without soil amendment (T2), rice sole cropping following FP with phosphogypsum (PG) amendment (T3), Rice-Shrimp mixed culture with PG amendment (T4), Rice-Crabs mixed culture with PG amendment (T5), Rice-Shrimp mixed culture + PG amendment with Spirulina (Cyanobacteria) inoculation (T6), and Rice-Crabs mixed culture + PG amendment with Spirulina inoculation (T7). A closed chamber technique was followed to collect gas samples from the rice paddy field and samples were analyzed by Gas Chromatograph. It was found that Rice-Shrimp (T6) and Rice-Crabs mixed farming (T7) practices significantly decreased GWPs compared to the rice sole cropping system. In the dry boro season, the maximum GWPs 4175 kg CO<sub>2</sub> eq. ha<sup>-1</sup> was recorded from rice sole cropping (T2), which was decreased by 30% and 36.7% under Rice-Shrimp (T6) and Rice-Crabs (T7) mixed farming practices, respectively. Furthermore, in the wet aman season, maximum GWP 4525 kg CO<sub>2</sub> eq. ha<sup>-1</sup> was recorded from rice sole cropping (T2), which was decreased by 33.0% and 38.8% under Rice-Shrimp and Rice-Crabs mixed farming, respectively. Rice grain yield was low under rice sole cropping (3500 kg/ha), which was increased by 11.0% and 14.7% under Rice-Shrimp mixed farming amended with PG and Spirulina (T6) during wet aman and dry boro seasons, respectively. The postharvest soil properties, such as soil organic matter content, redox potential value (Eh), and exchangeable K<sup>+</sup> and Ca<sup>2+</sup>, contents in soil increased significantly with Phosphogypsum and Spirulina applications, however, decreased Na<sup>+</sup> content and electrical conductivity (EC) eventually improved rice plants’ tolerance to salinity and enhanced overall productivity of Rice-Shrimp and Rice-Crabs mixed farming. Conclusively, the conversion of wetland mono rice cropping into mixed Rice-Shrimp and Rice-Crabs farming would be a feasible strategy to sustain rice aquaculture-based farming, ensure food security and mitigate GWPs in coastal wetlands ecosystem.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.52271273)the Open Foundation of the Key Laboratory of Ministry of Education for Coastal Disaster and Protection(Grant No.Z202201)。
文摘Coastal wetlands are hotspots for nitrogen(N)cycling,and crab burrowing is known to transform N in intertidal marsh soils.However,the underlying mechanisms remain unclear.This study conducted field experiments and used indoor control test devices to investigate the seasonal response of nitrogen to crab disturbance at the sediment-water interface in coastal tidal flat wetlands.The results showed that crab disturbance exhibited significant seasonality with large seasonal differences in cave density and depth.Due to crab disturbance,nitrogen fuxes at the sediment-water interface were much greater in the box with crabs than in the box without crabs.In summer,NH-N showed a positive flux from the sediment to the overlying water,but NO2-N and NOg-N showed positive fluxes from the sediment to the overlying water only in early stages.In winter,NH-N showed a positive flux from the sediment to the overlying water,but NO-N and NO,-N both exhibited positive and negative fluxes.These results indicated that the presence of crab burrows can cause the aerobic layer to move downward by approximately 8-15 cm in summer and directly promote nitrification at the sediment surface.
基金funded the World Class Research(WCR)Grant of Universitas Diponegoro with Contract Number 357-36/UN7.D2/PP/IV/2024.
文摘Hydroxyapatite(HA)is a bio ceramic commonly utilized in bone tissue engineering due to its bioactive and osteoconductive properties.Crab shells are usually disregarded as waste material despite their significant CaCO_(3) content,and have not been widely utilized in the synthesis of HA.This study aims to synthesize and analyze HA derived from crab shells using the hydrothermal method with different durations of holding time.This study utilized precipitated calcium carbonate(PCC)derived from crab shells.With a hydrothermal reactor set at 160℃ and varying holding times of 14(HA_14),16(HA_16),and 18(HA_18)h,a PCC and(NH4)2HPO4 mixture was used to synthesize HA.The synthesis results were analyzed using scanning electron microscopy(SEM),fourier transform infrared spectroscopy(FTIR),and X-ray diffraction(XRD)tests.This study has accomplished the synthesis of HA from crab shells.Nonetheless,the final product of synthesis still contained CaCO_(3) as an impurity.The prolonged hydrothermal holding time of 14 to 18 h resulted in a reduction of impurities while increasing the percentage of crystal weight and crystallite size of HA.Specimen CH_18 is the best-quality product generated in this study.This specimen produced HA with the highest percentage of crystal weight and crystallite size compared to the other specimens.Furthermore,specimen CH_18 exhibited the lowest concentration of impurities.The Ca/P ratio in this specimen was also the closest to 1.67.The Ca/P ratio,crystallite size,and crystal weight percentage of this specimen are 1.54,19.06 nm,and 99.1%,respectively.
基金supported by the National Natural Science Foundation of China (Nos. 61873196 and 62373030)the Innovation Program for Quantum Science and Technology(No. 2021ZD0303400)。
文摘In the two-dimensional positioning method of pulsars, the grid method is used to provide non-sensitive direction and positional estimates. However, the grid method has a high computational load and low accuracy due to the interval of the grid. To improve estimation accuracy and reduce the computational load, we propose a fast twodimensional positioning method for the crab pulsar based on multiple optimization algorithms(FTPCO). The FTPCO uses the Levenberg–Marquardt(LM) algorithm, three-point orientation(TPO) method, particle swarm optimization(PSO) and Newton–Raphson-based optimizer(NRBO) to substitute the grid method. First, to avoid the influence of the non-sensitive direction on positioning, we take an orbital error and the distortion of the pulsar profile as optimization objectives and combine the grid method with the LM algorithm or PSO to search for the non-sensitive direction. Then, on the sensitive plane perpendicular to the non-sensitive direction, the TPO method is proposed to fast search the sensitive direction and sub-sensitive direction. Finally, the NRBO is employed on the sensitive and sub-sensitive directions to achieve two-dimensional positioning of the Crab pulsar. The simulation results show that the computational load of the FTPCO is reduced by 89.4% and the positioning accuracy of the FTPCO is improved by approximately 38% compared with the grid method. The FTPCO has the advantage of high real-time accuracy and does not fall into the local optimum.
文摘The science of radio astronomy focuses on the observation and study of celestial objects by reading their radio waves. The 5 meter radio-telescope is able to observe different radio sources using a C-band LNB. This research was essentially focused on Crab Nebula, also known as Taurus A. The study led to interesting observations, which were validated numerically using various scientific computing software. The radio waves emitted by Taurus A are readable by the RTL-SDR, a software defined radio receiver. This device is capable of reading radio frequencies in the range of 0.5 MHZ to 1700 MHZ.
文摘通过对比NICER(Neutron Star Interior Composition Explorer)和XPNAV-1(X-ray Pulsar Navigation-1)关于Crab脉冲星同一时段(108天)观测数据的计时处理结果,发现在周期跃变发生前的时段(95天),NICER数据的拟合前计时残差的RMS(root mean square)为5.77μs,远优于XPNAV-1数据的拟合前计时残差的RMS 51.56μs,体现了NICER在有效面积、探测效率、数据采集等方面的优势,给未来我国的X射线脉冲星探测器研制提供了发展方向;而在周期跃变发生后的时段(13天),发现XPNAV-1数据的拟合前计时残差的RMS为55.87μs,而NICER数据的拟合前计时残差的RMS为167.27μs,周期跃变对NICER的影响更大,说明在处理周期跃变发生后时段的NICER数据时,由于NICER的观测精度非常高,需要更频繁地更新Crab星历。最后分别得到了两个探测器整段数据的计时残差。XPNAV-1数据的拟合前计时残差的RMS为55.94μs,而NICER数据的拟合前计时残差的RMS为64.34μs,这说明NICER数据受周期跃变影响更为明显,进一步证明了上述结论。
基金supported by the earmarked fund for the Anhui Provincial Modern Agri-industry Technology Research System(AHCYJSTX-08)the China Agriculture Research System of MOF and MARA(CARS-48)。
文摘The tropomyosin(TM)fractions of crab protems may cause allergic reactions in mdividuals susceptible to allergies;however,efficient and safe methods by which to reduce such allergenicity are not currently available.Therefore,in this study,the effects of three different processing methods,i.e.,microwave,ultrasound,and high temperature-pressure(HTP)treatments,on the digestion stability of TM from Chinese mitten crab muscle and the allergenicity of TM digestion products were explored.sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that microwaving had little effect on the digestion stability of TM.In contrast,ultrasound and HTP treatments facilitated the degradation of TM.Similarly,Western blotting and inhibition ELISA indicated that the IgE-binding activity of TM was significantly reduced after treatment with ultrasound or HTP.Among the three different proces sing methods,HTP was the most effective method for improving digestibility of TM and reducing immunoreactivity.This finding provides new insights into treatments for crab allergies.
文摘对于脉冲星试验01星的两年实测数据,针对蟹状星云脉冲星星历经历了多次更新的特点,提出了更新标准脉冲轮廓进行X射线计时的改进方法。以一系列观测时长为实测数据分组,再采用改进后的方法进行计时分析并与改进前的结果进行对比。通过对比改进前后的计时结果,发现改进方法得到的计时残差的均方根(root mean square, RMS)平均降低了14.2%,定位精度平均提高了14.4%,估计原子钟相对频率偏差的精度平均提高了26.2%,体现了改进方法处理经历多次星历更新的Crab脉冲星观测数据的合理性和优越性。此外,改进方法对脉冲星试验01星两年观测数据的稳定度σ_(z)没有量级上的改进,说明改进方法对Crab脉冲星时间稳定度的影响不大。
文摘The aim of the present study was to determine the MICs using broth microdilution method and their combination effect by (minimum inhibitory concentrations) of selected antibiotic agents determination of FICindex (fractional inhibitory concentrations). Lysozyme Anodonta cygnea was more active than Lysozyme Uniopictorum, especially against Gram-negative bacterias. Combination of Macropen and Lysozyme Unio pictorum has a synergistic effect, while a separation of these antibacterial agents was not active against some of the strains of microorganisms.
基金the National Natural Science Foundation of China(Nos.11975298 and 12175292).
文摘A diffraction-limited storage ring with a multi-bend achromat lattice suffers from a small dynamic aperture for conventional off-axis injection.Thus,a longitudinal on-axis injection scheme based on a new type of crab cavity is proposed in this paper.Particle tracking simulations were performed to study the disturbance of the stored beam and the motion of the injected beam during the injection process.The possibility of multi-bunch injections was discussed.In addition,the effect of the long-range wake field induced by the stored beam was analyzed.A C-band standing-wave crab cavity was designed and produced as requested,and its field distribution was measured.The corresponding results are consistent with the simulation results.
基金Supported by the National Key R&D Program of China (No.2018YFD0901301)the Natural Science Foundation of Shandong Province (No.ZR2020MC189)+1 种基金the Fundamental Research Funds for Central Universities of China (No.201822018)the Pilot National Laboratory for Marine Science and Technology (Qingdao) (No.JCZX202024)。
文摘Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of crabs.However,no continuous crab cell line has been available due to the lacking of suitable medium and the occurrence of mitosis-arrest.In this study,long-term in vitro culture conditions for both two-(2D)and three-dimensions(3D)were successfully developed for the circulating hemocytes of swimming crab Portunus trituberculatus,designated as PTH cells.In 2D culture,a novel crab basic medium in osmolarity of 990–1100 mOsm/kg was optimized for the first time,which is different from Leibovitz's L-15 medium in mainly the components of amino acids,containing double strengths of the contents of free amino acid mixture in the crab serum.Then an optimal crab growth medium was developed by supplementing 5%fetal bovine serum,50-g/L yeast extract powder,20-μg/L basic fibroblast growth factor and epidermal growth factor into the optimal crab basic medium,and found that it could support a long-term survival of PTH cells in a healthy monolayer up to 347 days and partially break through the mitosis-arrest of crab cells evidenced by the obvious increase of proliferating potential detected in the 10-d primarily cultured PTH cells.These 2D cultured PTH cells could be successfully sub-cultured for 11 times by physical flushing method and well cryopreserved in liquid nitrogen.In 3D culture,using the same crab growth medium,the PTH cell aggregates could be easily formed and healthily maintained on the surface of solidified Matrigel or in the ultra-low-attachment plate with a survival rate of 50%–60%on Day 103.This work largely improved the primary culture and subculture of crab cells and will facilitate the establishment of continuous crab cell line.
文摘Coastal agriculture is vulnerable to climate change, thereby may affect food production systems and food security in Bangladesh. Methane (CH4) emission from coastal wetlands rice farming is a major environmental concern due to its global warming potential. Therefore, field experiments were conducted at the southern coastal region of Shyamnagar, Satkhira, to investigate the feasibility of Rice-Shrimp and Rice-Crabs mixed farming for adaptation to the changing climate and sustaining food production system. The experimental treatments were designed on rice-based diversified farming systems such as rice sole cropping with no NPKS + no soil amendments (T1), rice sole cropping following farmers’ practice (FP) without soil amendment (T2), rice sole cropping following FP with phosphogypsum (PG) amendment (T3), Rice-Shrimp mixed culture with PG amendment (T4), Rice-Crabs mixed culture with PG amendment (T5), Rice-Shrimp mixed culture + PG amendment with Spirulina (Cyanobacteria) inoculation (T6), and Rice-Crabs mixed culture + PG amendment with Spirulina inoculation (T7). A closed chamber technique was followed to collect gas samples from the rice paddy field and samples were analyzed by Gas Chromatograph. It was found that Rice-Shrimp (T6) and Rice-Crabs mixed farming (T7) practices significantly decreased GWPs compared to the rice sole cropping system. In the dry boro season, the maximum GWPs 4175 kg CO<sub>2</sub> eq. ha<sup>-1</sup> was recorded from rice sole cropping (T2), which was decreased by 30% and 36.7% under Rice-Shrimp (T6) and Rice-Crabs (T7) mixed farming practices, respectively. Furthermore, in the wet aman season, maximum GWP 4525 kg CO<sub>2</sub> eq. ha<sup>-1</sup> was recorded from rice sole cropping (T2), which was decreased by 33.0% and 38.8% under Rice-Shrimp and Rice-Crabs mixed farming, respectively. Rice grain yield was low under rice sole cropping (3500 kg/ha), which was increased by 11.0% and 14.7% under Rice-Shrimp mixed farming amended with PG and Spirulina (T6) during wet aman and dry boro seasons, respectively. The postharvest soil properties, such as soil organic matter content, redox potential value (Eh), and exchangeable K<sup>+</sup> and Ca<sup>2+</sup>, contents in soil increased significantly with Phosphogypsum and Spirulina applications, however, decreased Na<sup>+</sup> content and electrical conductivity (EC) eventually improved rice plants’ tolerance to salinity and enhanced overall productivity of Rice-Shrimp and Rice-Crabs mixed farming. Conclusively, the conversion of wetland mono rice cropping into mixed Rice-Shrimp and Rice-Crabs farming would be a feasible strategy to sustain rice aquaculture-based farming, ensure food security and mitigate GWPs in coastal wetlands ecosystem.