Analysis of Hupehenine in the Total Alkaloids from Fritillaria hupehensis by HPLC-ELSD

A reverse-phase high pressure liquid chromatography （HPLC） method with evaporative light scattering detection （ELSD） has been developed for the quantitative analysis of hupehenine in the total alkaloids from Fritillaria hupehensis. Samples were analyzed on a reverse-phase column （Hypersil C-18） with a mobile phase of methanol：water：chloroform： triethylamine （85：15：1：0.6）. The ELSD was set at the drift tube temperature of 68.3℃ and gas flow rate of 1.8 L/min. Hupehenine＇s retention time was 13.7 min with an asymmetry factor of 1.2. The validity of the method has been verified with linearity, limit of detection, accuracy and precision. The logarithmic linear curve was obtained from 8.936 to 134.04 μg/mL （r=0.9993）. The detection limit （S/N〉3） of hupehenine was 1.79 μg/mL on the column. Intra-day RSD was 1.42% and inter-day RSD was 2.26% （3 days within a week）. The average recovery of hupehenine was 101.50%, and RSD was 1.62%.

A new veratramine alkaloid from the bulbs of Fritillaria hupehensis

A new highly conjugated alkaloid of veratramine type, 22S,25S,5α-veratramine-7(8),12(14)-diene-3β,13β,23β-triol-6-one (1), was isolated from the bulbs of Fritillaria hupehensis Hsiao et K.C. Hsia. Its structure was determined on the basis of spectroscopic evidences.

A new cycloartane triterpenoid from the leaves and stems of Fritillaria hupehensis

A new triterpenoid has been isolated from the leaves and stems of Fritillaria hupehensis Hsiao et K.C. Hsia. Its structure was established as （2329-9,19-cycloart-23-ene-3α,25-diol 1 through chemical and spectroscopic studies including 2D NMR. Another known triterpenoid 9,19-cycloart-25-ene-313,24ζ-diol 2 was also isolated.

Alkaloids from Fritillaria Hupehensis

Phytochemical investigation of the bulbs of Fritillaria hupehensis resulted in the isolation and structural elucidation of a new highly conjugated alkaloid of veratramine type identified as 3β,23β-dihydroxy-7,12（14）-dien-5α- veratramin-6-one （1）, together with two known alkaloids, ebeinine （2） and zhebeinine （3）, which were isolated for the first time from F. hupehensis. The structures of alkaloids 1-3 were established by extensive 1D and 2D NMR spectral analyses.

湖北贝母石油醚段的抗菌活性和化学成分研究

为探究湖北贝母(Fritillaria hupehensis Hsiao et k.c.Hsia)石油醚段的抗菌活性和化学成分,将湖北贝母经60%乙醇溶液超声提取获得总浸膏,再采用石油醚萃取获得石油醚段浸膏,利用滤纸片法、稀释倒平板法、超微量紫外分光光度计以及电导仪对湖北贝母石油醚段的抗菌活性和机制进行了探索;同时,利用GC-MS、1D和2D NMR等对湖北贝母石油醚段的成分进行了探索。结果表明,湖北贝母石油醚段溶液对于苏云金芽孢杆菌、绿脓杆菌有中度敏感抑制活性,对肺炎克雷伯杆菌ATCC13883、金黄色葡萄球菌ATCC29213、枯草芽孢杆菌有轻度敏感抑制活性。其中,只有苏云金芽孢杆菌、枯草芽孢杆菌、绿脓杆菌的MIC/MBC小于4.0 mg/mL。湖北贝母石油醚段很可能通过破坏细胞膜从而减缓了苏云金芽孢杆菌、枯草芽孢杆菌、绿脓杆菌生长,从而达到抗菌的效果,并获得了对应总离子流中的27种化合物。