In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamm...In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamma-ray measurements and improve computational efficiency,an improved shuffled frog leaping algorithm-particle swarm optimization convolutional neural network(SFLA-PSO CNN)for large-sample quantitative analysis of airborne gamma-ray spectra is proposed herein.This method was used to train the weight of the neural network,optimize the structure of the network,delete redundant connections,and enable the neural network to acquire the capability of quantitative spectrum processing.In full-spectrum data processing,this method can perform the functions of energy spectrum peak searching and peak area calculations.After network training,the mean SNR and RMSE of the spectral lines were 31.27 and 2.75,respectively,satisfying the demand for noise reduction.To test the processing ability of the algorithm in large samples of airborne gamma spectra,this study considered the measured data from the Saihangaobi survey area as an example to conduct data spectral analysis.The results show that calculation of the single-peak area takes only 0.13~0.15 ms,and the average relative errors of the peak area in the U,Th,and K spectra are 3.11,9.50,and 6.18%,indicating the high processing efficiency and accuracy of this algorithm.The performance of the model can be further improved by optimizing related parameters,but it can already meet the requirements of practical engineering measurement.This study provides a new idea for the full-spectrum processing of airborne gamma rays.展开更多
The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-d...The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-dominated sorting genetic algorithm-II(NSGA-II)called the non-dominated shuffled frog leaping algorithm(NSFLA)is proposed to maximize damage to enemy targets and minimize the self-threat in air combat constraints.In NSFLA,the shuffled frog leaping algorithm(SFLA)is introduced to NSGA-II to replace the inside evolutionary scheme of the genetic algorithm(GA),displaying low optimization speed and heterogeneous space search defects.Two improvements have also been raised to promote the internal optimization performance of SFLA.Firstly,the local evolution scheme,a novel crossover mechanism,ensures that each individual participates in updating instead of only the worst ones,which can expand the diversity of the population.Secondly,a discrete adaptive mutation algorithm based on the function change rate is applied to balance the global and local search.Finally,the scheme is verified in various air combat scenarios.The results show that the proposed NSFLA has apparent advantages in solution quality and efficiency,especially in many aircraft and the dynamic air combat environment.展开更多
Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subject...Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subjected to modifications,the drastic increase in the count of test cases forces the testers to opt for a test optimization strategy.One such strategy is test case prioritization(TCP).Existing works have propounded various methodologies that re-order the system-level test cases intending to boost either the fault detection capabilities or the coverage efficacy at the earliest.Nonetheless,singularity in objective functions and the lack of dissimilitude among the re-ordered test sequences have degraded the cogency of their approaches.Considering such gaps and scenarios when the meteoric and continuous updations in the software make the intensive unit and integration testing process more fragile,this study has introduced a memetics-inspired methodology for TCP.The proposed structure is first embedded with diverse parameters,and then traditional steps of the shuffled-frog-leaping approach(SFLA)are followed to prioritize the test cases at unit and integration levels.On 5 standard test functions,a comparative analysis is conducted between the established algorithms and the proposed approach,where the latter enhances the coverage rate and fault detection of re-ordered test sets.Investigation results related to the mean average percentage of fault detection(APFD)confirmed that the proposed approach exceeds the memetic,basic multi-walk,PSO,and optimized multi-walk by 21.7%,13.99%,12.24%,and 11.51%,respectively.展开更多
Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involve...Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involves more than rendering words;it entails navigating cultural differences and facilitating literary communication during the process of language conversion.By comparing and contrasting Mo Yan’s work of“蛙”with Howard Goldblatt’s English translation Frog from both the two aspects of language and culture and the four perspectives of alienation and naturalization,deletion and addition,processing of address and dialogues,symbols of animal cultural,this study explores the phenomenon of creative treason in literary translation,and provides a specific and in-depth analysis to offer valuable insights for the translation of Chinese literature.展开更多
The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields o...The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields of 8 regions in Heilongjiang Province. Totally 78 polymorphic DNA loci were amplified by 10 RAP D primers. By genetic distance analysis and phylogenetic tree reconstruction wit h the Neighbor-Joining (NJ) method the results showed that the populations of Ch inese forest frogs distributed in 8 regions existed great differentiation (Avera ge Fst=0.347, SD=0.235) while there was the paradox between geographic distances and genetic distances. Based on geographic and geological data, a hypothesis wa s posed that it is very possible that the hilly lands in the downstream of the S onghua River and the Heilong River were the center of the origin of the Chinese forest frogs in Heilongjiang Province. And mainly through the Songhua River syst em, the Chinese forest frogs dispersed into the Songneng Plain from the Sanjiang Plain whereas the Fangzheng region became a sub-center for the western dispersi on.展开更多
The genetic diversity and population structure of the Dybowski's frog(Rana dybowskii) were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser K...The genetic diversity and population structure of the Dybowski's frog(Rana dybowskii) were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains,China.Results showed that allele number of the 11 microsatellite loci was in the range of 2-10 in all populations,with the mean of 5.6.The average expected heterozygosity(HE) was 0.572,indicating a moderate polymorphism.The results of genetic differentiation coefficient(FST) showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains(p〈0.001).This result was verified further by Nei's genetic distance(DA) based on UPGMA phy-logenetic trees and by AMOVA analysis.In conclusion,the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units(MUs) for their protection and management.展开更多
Attribute reduction in the rough set theory is an important feature selection method, but finding a minimum attribute reduction has been proven to be a non-deterministic polynomial (NP)-hard problem. Therefore, it i...Attribute reduction in the rough set theory is an important feature selection method, but finding a minimum attribute reduction has been proven to be a non-deterministic polynomial (NP)-hard problem. Therefore, it is necessary to investigate some fast and effective approximate algorithms. A novel and enhanced quantum-inspired shuffled frog leaping based minimum attribute reduction algorithm (QSFLAR) is proposed. Evolutionary frogs are represented by multi-state quantum bits, and both quantum rotation gate and quantum mutation operators are used to exploit the mechanisms of frog population diversity and convergence to the global optimum. The decomposed attribute subsets are co-evolved by the elitist frogs with a quantum-inspired shuffled frog leaping algorithm. The experimental results validate the better feasibility and effectiveness of QSFLAR, comparing with some representa- tive algorithms. Therefore, QSFLAR can be considered as a more competitive algorithm on the efficiency and accuracy for minimum attribute reduction.展开更多
The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel i...The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel is explored here.That is,the abnormal social phenomena exist in the reality,the intrigue among people to gain profits and also the immoral measures people took for panning gold at the Gold Rush Era.展开更多
This paper presents a mechanical model of jumping robot based on the biological mechanism analysis of frog. By biological observation and kinematic analysis the frog jump is divided into take-offphase, aerial phase an...This paper presents a mechanical model of jumping robot based on the biological mechanism analysis of frog. By biological observation and kinematic analysis the frog jump is divided into take-offphase, aerial phase and landing phase. We find the similar trajectories of hindlimb joints during jump, the important effect of foot during take-off and the role of forelimb in supporting the body. Based on the observation, the frog jump is simplified and a mechanical model is put forward. The robot leg is represented by a 4-bar spring/linkage mechanism model, which has three Degrees of Freedom (DOF) at hip joint and one DOF (passive) at tarsometatarsal joint on the foot. The shoulder and elbow joints each has one DOF for the balancing function of arm. The ground reaction force of the model is analyzed and compared with that of frog during take-off. The results show that the model has the same advantages of low likelihood of premature lift-off and high efficiency as the frog. Analysis results and the model can be employed to develop and control a robot capable of mimicking the jumping behavior of frog.展开更多
Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, how...Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, however, among species. Nevertheless, the causal factors influencing circadian variations of calling patterns in anuran species are not clear. In this study, male chorus vocalizations were monitored in the Emei music frog(Babina daunchina) for 17 consecutive days during the breeding season, while its habitat air temperature and relative humidity in the course of experiments were measured as well. The results revealed that the circadian calling patterns were characterized by two periods of peak vocalization, which were observed from 0500 h to 0700 h and from 1300 h to 2000 h, while the lowest activity period was found from 2100 h to 2200 h. Both calls/h and notes/h were positively correlated with air temperature and negatively with relative humidity. Overall, our data indicate that the Emei music frogs(B. daunchina) could regulate their vocal activities based on the changes of physical micro-environment(e. g., temperature or humidity) to maximize reproductive success.展开更多
The giant spiny frog (Quasipaa spinosa) is an endangered species with a relatively small distribution limited to southern China and Northern Vietnam. This species is becoming increasingly threatened because of over-...The giant spiny frog (Quasipaa spinosa) is an endangered species with a relatively small distribution limited to southern China and Northern Vietnam. This species is becoming increasingly threatened because of over-exploitation and habitat degradation. This study provides data on the genetic diversity and population genetic structure of the giant spiny frog to facilitate the further development of effective conservation recommendations for this economically important but threatened species. We examined 10 species-specific microsatellite loci and Cyt b genes (562 bp) collected from 13 wild populations across the entire range of this species. Results of 10 microsatellite loci analysis showed a generally high level of genetic diversity. Moreover, the genetic differentiation among all 12 populations was moderate to large (overall Fs7= 0.1057). A total of 51 haplotypes were identified for Cyt b, which suggests high haplotype nucleotide diversities. Phylogeographic and population structure analyses using both DNA markers suggested that the wild giant spiny frog can be divided into four distinct major clades, i.e., Northern Vietnam, Western China, Central China, and Eastern China. The clades with significant genetic divergence are reproductively isolated, as evidenced by a high number of private alleles and strong incidence of failed amplification in microsatellite loci. Our research, coupled with other studies, suggests that Q. spinosa might be a species complex within which no detectable morphological variation has been revealed. The four phylogenetic clades and some subclades with distinct geographical distribution should be regarded as independent management units for conservation purposes.展开更多
Commercial farming of anuran species that are declining in the wild raises a need to discriminate wild from farmed frogs.We hypothesized wild frogs might have extended hindlimbs due to greater frequency or intensity o...Commercial farming of anuran species that are declining in the wild raises a need to discriminate wild from farmed frogs.We hypothesized wild frogs might have extended hindlimbs due to greater frequency or intensity of jumping relative to farmed frogs,highlighting a morphometric approach to discrimination of wild from farmed frogs using hindlimb length.In the present study,Dybowski's frog(Rana dybowskii) was used to test this hypothesis.We measured body mass(Mb) and hindlimb length(Lh) of 2-year old farmed frogs and wild frogs aged 2 to 5 years.Dybowski's frog demonstrated significant dimorphism in Mb and Lh.Mb was significantly greater among farmed 2-year old frogs in both sexes(p=0.000),while only among females was Lh significantly greater for wild frogs(p=0.000).Lh/Mb was used as an index for origin discrimination to eliminate the influence of Mb due to variation of husbandry conditions among farms.Mean Lh/Mb for farmed frogs was significantly lower than for wild frogs(p=0.000) in the 2-year old age class.Discrimination correctly classified 84.4% of farmed and 96.3% of wild male frogs.Among females,92.9% of farmed frogs and 90.1% wild frogs were correctly classified.The overall correctness of classification was 92.1% and 90.8% for males and females,respectively.However,Lh/Mb revealed variation with age,resulting in reduced discriminative power for frogs ≥3 years old.We introduced a coefficient Ce to adjust the Lh/Mb of frogs ≥3 years to the level equivalent to 2-year frogs.The adjustment achieved 89.5% for overall correctness of origin for wild males and 92.4% for wild females ≥3 years old.These results show that Lh/Mb is an effective index to discriminate wild from farmed Dybowski's frog.Since the physical demands of jumping are common among anurans,this index is also potentially applicable to other anuran species.展开更多
Changes in distribution density,morphology and secretory content of endocrine cells in the gastro-entero-pancreatic system of black-spotted frogs Rana nigromaculata before and after fasting were investigated using imm...Changes in distribution density,morphology and secretory content of endocrine cells in the gastro-entero-pancreatic system of black-spotted frogs Rana nigromaculata before and after fasting were investigated using immunohistochemistry and antisera to six gut hormones.Six types of endocrine cells were detected in the digestive tract of Rana nigromaculata,including 5-hydroxytryptamine(5-HT),gastrin(GAS),somatostatin(SOM),glucagon(GLU),pancreatic polypeptide(PP)and vasoactive intestinal polypeptide(VIP)cells.After fasting,the density of 5-HT cells in the esophagus,cardia and fundus,GAS cells in the fundus and pylorus,PP cells in the fundus decreased significantly(P<0.01),while SOM cells in the cardia,GLU cells in the rectum increased significantly(P<0.01).The cytoplasmic processes of 5-HT cells became shorter or not detectable.The secretory content of GAS cells reduced in the cardia.The positive immunostaining reaction in the perinuclear region of SOM cells in the cardia,fundus and pylorus became weaker,while the staining intensity in the periphery of these cells became stronger.VIP cells were not detectable in the whole digestive tract after fasting.Five types of endocrine cells were found in the pancreas of Rana nigromaculata,including 5-HT,GAS,SOM,GLU and PP cells.After fasting,the density of 5-HT cells decreased slightly(P>0.05),while SOM,GAS,GLU and PP cells increased significantly(P<0.01).Furthermore,the secretory content of GLU cells increased significantly.Considering their functionalities,our results indicate that the changes of GEP endocrine cells in Rana nigromaculata responded adaptively to starvation-induced stress.展开更多
Pneumatic muscles with similar characteristics to biological muscles have been widely used in robots, and thus are promising drivers for frog inspired robots. How- ever, the application and nonlinearity of the pneumat...Pneumatic muscles with similar characteristics to biological muscles have been widely used in robots, and thus are promising drivers for frog inspired robots. How- ever, the application and nonlinearity of the pneumatic system limit the advance. On the basis of the swimming mechanism of the frog, a frog-inspired robot based on pneumatic muscles is developed. To realize the indepen- dent tasks by the robot, a pneumatic system with internal chambers, micro air pump, and valves is implemented. The micro pump is used to maintain the pressure difference between the source and exhaust chambers. The pneumatic muscles are controlled by high-speed switch valves which can reduce the robot cost, volume, and mass. A dynamic model of the pneumatic system is established for the sim- ulation to estimate the system, including the chamber, muscle, and pneumatic circuit models. The robot design is verified by the robot swimming experiments and the dynamic model is verified through the experiments and simulations of the pneumatic system. The simulation results are compared to analyze the functions of the source pressure, internal volume of the muscle, and circuit flow rate which is proved the main factor that limits the response of muscle pressure. The proposed research provides the application of the pneumatic muscles in the frog inspired robot and the pneumatic model to study muscle controller.展开更多
基金the National Natural Science Foundation of China(No.42127807)Natural Science Foundation of Sichuan Province(Nos.23NSFSCC0116 and 2022NSFSC12333)the Nuclear Energy Development Project(No.[2021]-88).
文摘In airborne gamma ray spectrum processing,different analysis methods,technical requirements,analysis models,and calculation methods need to be established.To meet the engineering practice requirements of airborne gamma-ray measurements and improve computational efficiency,an improved shuffled frog leaping algorithm-particle swarm optimization convolutional neural network(SFLA-PSO CNN)for large-sample quantitative analysis of airborne gamma-ray spectra is proposed herein.This method was used to train the weight of the neural network,optimize the structure of the network,delete redundant connections,and enable the neural network to acquire the capability of quantitative spectrum processing.In full-spectrum data processing,this method can perform the functions of energy spectrum peak searching and peak area calculations.After network training,the mean SNR and RMSE of the spectral lines were 31.27 and 2.75,respectively,satisfying the demand for noise reduction.To test the processing ability of the algorithm in large samples of airborne gamma spectra,this study considered the measured data from the Saihangaobi survey area as an example to conduct data spectral analysis.The results show that calculation of the single-peak area takes only 0.13~0.15 ms,and the average relative errors of the peak area in the U,Th,and K spectra are 3.11,9.50,and 6.18%,indicating the high processing efficiency and accuracy of this algorithm.The performance of the model can be further improved by optimizing related parameters,but it can already meet the requirements of practical engineering measurement.This study provides a new idea for the full-spectrum processing of airborne gamma rays.
基金supported by the National Natural Science Foundation of China(61673209,71971115)。
文摘The dynamic weapon target assignment(DWTA)problem is of great significance in modern air combat.However,DWTA is a highly complex constrained multi-objective combinatorial optimization problem.An improved elitist non-dominated sorting genetic algorithm-II(NSGA-II)called the non-dominated shuffled frog leaping algorithm(NSFLA)is proposed to maximize damage to enemy targets and minimize the self-threat in air combat constraints.In NSFLA,the shuffled frog leaping algorithm(SFLA)is introduced to NSGA-II to replace the inside evolutionary scheme of the genetic algorithm(GA),displaying low optimization speed and heterogeneous space search defects.Two improvements have also been raised to promote the internal optimization performance of SFLA.Firstly,the local evolution scheme,a novel crossover mechanism,ensures that each individual participates in updating instead of only the worst ones,which can expand the diversity of the population.Secondly,a discrete adaptive mutation algorithm based on the function change rate is applied to balance the global and local search.Finally,the scheme is verified in various air combat scenarios.The results show that the proposed NSFLA has apparent advantages in solution quality and efficiency,especially in many aircraft and the dynamic air combat environment.
文摘Both unit and integration testing are incredibly crucial for almost any software application because each of them operates a distinct process to examine the product.Due to resource constraints,when software is subjected to modifications,the drastic increase in the count of test cases forces the testers to opt for a test optimization strategy.One such strategy is test case prioritization(TCP).Existing works have propounded various methodologies that re-order the system-level test cases intending to boost either the fault detection capabilities or the coverage efficacy at the earliest.Nonetheless,singularity in objective functions and the lack of dissimilitude among the re-ordered test sequences have degraded the cogency of their approaches.Considering such gaps and scenarios when the meteoric and continuous updations in the software make the intensive unit and integration testing process more fragile,this study has introduced a memetics-inspired methodology for TCP.The proposed structure is first embedded with diverse parameters,and then traditional steps of the shuffled-frog-leaping approach(SFLA)are followed to prioritize the test cases at unit and integration levels.On 5 standard test functions,a comparative analysis is conducted between the established algorithms and the proposed approach,where the latter enhances the coverage rate and fault detection of re-ordered test sets.Investigation results related to the mean average percentage of fault detection(APFD)confirmed that the proposed approach exceeds the memetic,basic multi-walk,PSO,and optimized multi-walk by 21.7%,13.99%,12.24%,and 11.51%,respectively.
文摘Literary translation should not solely focus on“what to translate”,but also on“how to translate”,extending the perspective to the target audience and environment.It is crucial to recognize that translation involves more than rendering words;it entails navigating cultural differences and facilitating literary communication during the process of language conversion.By comparing and contrasting Mo Yan’s work of“蛙”with Howard Goldblatt’s English translation Frog from both the two aspects of language and culture and the four perspectives of alienation and naturalization,deletion and addition,processing of address and dialogues,symbols of animal cultural,this study explores the phenomenon of creative treason in literary translation,and provides a specific and in-depth analysis to offer valuable insights for the translation of Chinese literature.
基金the Natural Science Foundation of Heilongjiang Province (C9726).
文摘The Random Amplified Polymorphism DNA (RAPD) markers were used to stu dy the intra-specific diversity and regional differentiation of the Chinese fore st frogs (Rana chensinensis), which were sampled from the fields of 8 regions in Heilongjiang Province. Totally 78 polymorphic DNA loci were amplified by 10 RAP D primers. By genetic distance analysis and phylogenetic tree reconstruction wit h the Neighbor-Joining (NJ) method the results showed that the populations of Ch inese forest frogs distributed in 8 regions existed great differentiation (Avera ge Fst=0.347, SD=0.235) while there was the paradox between geographic distances and genetic distances. Based on geographic and geological data, a hypothesis wa s posed that it is very possible that the hilly lands in the downstream of the S onghua River and the Heilong River were the center of the origin of the Chinese forest frogs in Heilongjiang Province. And mainly through the Songhua River syst em, the Chinese forest frogs dispersed into the Songneng Plain from the Sanjiang Plain whereas the Fangzheng region became a sub-center for the western dispersi on.
文摘The genetic diversity and population structure of the Dybowski's frog(Rana dybowskii) were investigated by using 11 polymorphic microsatellite loci.Total 75 individuals were sampled from six populations in Lesser Khingan Mountains and Changbai Mountains,China.Results showed that allele number of the 11 microsatellite loci was in the range of 2-10 in all populations,with the mean of 5.6.The average expected heterozygosity(HE) was 0.572,indicating a moderate polymorphism.The results of genetic differentiation coefficient(FST) showed that population genetic differentiation was significant between Changbai and Lesser Khingan Mountains(p〈0.001).This result was verified further by Nei's genetic distance(DA) based on UPGMA phy-logenetic trees and by AMOVA analysis.In conclusion,the populations distributed in Lesser Khingan Mountains and Changbai Mountain are proposed to be two distinct management units(MUs) for their protection and management.
基金supported by the National Natural Science Foundation of China(6113900261171132)+4 种基金the Funding of Jiangsu Innovation Program for Graduate Education(CXZZ11 0219)the Natural Science Foundation of Jiangsu Education Department(12KJB520013)the Applying Study Foundation of Nantong(BK2011062)the Open Project Program of State Key Laboratory for Novel Software Technology,Nanjing University(KFKT2012B28)the Natural Science Pre-Research Foundation of Nantong University(12ZY016)
文摘Attribute reduction in the rough set theory is an important feature selection method, but finding a minimum attribute reduction has been proven to be a non-deterministic polynomial (NP)-hard problem. Therefore, it is necessary to investigate some fast and effective approximate algorithms. A novel and enhanced quantum-inspired shuffled frog leaping based minimum attribute reduction algorithm (QSFLAR) is proposed. Evolutionary frogs are represented by multi-state quantum bits, and both quantum rotation gate and quantum mutation operators are used to exploit the mechanisms of frog population diversity and convergence to the global optimum. The decomposed attribute subsets are co-evolved by the elitist frogs with a quantum-inspired shuffled frog leaping algorithm. The experimental results validate the better feasibility and effectiveness of QSFLAR, comparing with some representa- tive algorithms. Therefore, QSFLAR can be considered as a more competitive algorithm on the efficiency and accuracy for minimum attribute reduction.
文摘The Celebrated Jumping Frog of Calaveras County is a novel first published in 1865.It tells us a story taking place in the ancient mining camp of Angel's.By using analysis,the implication hidden behind the novel is explored here.That is,the abnormal social phenomena exist in the reality,the intrigue among people to gain profits and also the immoral measures people took for panning gold at the Gold Rush Era.
基金the National High Technology Research and Development Program of China (No.2006AA04Z245)Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) (IRT0423)
文摘This paper presents a mechanical model of jumping robot based on the biological mechanism analysis of frog. By biological observation and kinematic analysis the frog jump is divided into take-offphase, aerial phase and landing phase. We find the similar trajectories of hindlimb joints during jump, the important effect of foot during take-off and the role of forelimb in supporting the body. Based on the observation, the frog jump is simplified and a mechanical model is put forward. The robot leg is represented by a 4-bar spring/linkage mechanism model, which has three Degrees of Freedom (DOF) at hip joint and one DOF (passive) at tarsometatarsal joint on the foot. The shoulder and elbow joints each has one DOF for the balancing function of arm. The ground reaction force of the model is analyzed and compared with that of frog during take-off. The results show that the model has the same advantages of low likelihood of premature lift-off and high efficiency as the frog. Analysis results and the model can be employed to develop and control a robot capable of mimicking the jumping behavior of frog.
基金supported by the National Natural Science Foundation of China(30900141)the Chinese Academy of Sciences(KSCX2-EW-J-22,09C302,Y0B3011)the Chinese Academy of Sciences(KSCX2-YW-R-077)
文摘Generally, the function of vocalizations made by male anurans are to attract females or defend resources. Typically, males vocalize in choruses during one or more periods in a twenty-four-hour cycle, which varies, however, among species. Nevertheless, the causal factors influencing circadian variations of calling patterns in anuran species are not clear. In this study, male chorus vocalizations were monitored in the Emei music frog(Babina daunchina) for 17 consecutive days during the breeding season, while its habitat air temperature and relative humidity in the course of experiments were measured as well. The results revealed that the circadian calling patterns were characterized by two periods of peak vocalization, which were observed from 0500 h to 0700 h and from 1300 h to 2000 h, while the lowest activity period was found from 2100 h to 2200 h. Both calls/h and notes/h were positively correlated with air temperature and negatively with relative humidity. Overall, our data indicate that the Emei music frogs(B. daunchina) could regulate their vocal activities based on the changes of physical micro-environment(e. g., temperature or humidity) to maximize reproductive success.
基金supported by the National Science Foundation of China(No.31172116 and No.31472015)the Major Science and Technology Specific Projects of Zhejiang Province of China(No.2010C12008)+2 种基金the project of the Science Technology Commission of Zhejiang Province of China(No.2011C22006)the Found for the science and technology innovation team of Zhejiang Province of China(No.2012R10026-07)the Key Program of the Twelfth Five Year New Aquatic Varieties Breeding Cooperation of Zhejiang Province of China(2012C12907-9)
文摘The giant spiny frog (Quasipaa spinosa) is an endangered species with a relatively small distribution limited to southern China and Northern Vietnam. This species is becoming increasingly threatened because of over-exploitation and habitat degradation. This study provides data on the genetic diversity and population genetic structure of the giant spiny frog to facilitate the further development of effective conservation recommendations for this economically important but threatened species. We examined 10 species-specific microsatellite loci and Cyt b genes (562 bp) collected from 13 wild populations across the entire range of this species. Results of 10 microsatellite loci analysis showed a generally high level of genetic diversity. Moreover, the genetic differentiation among all 12 populations was moderate to large (overall Fs7= 0.1057). A total of 51 haplotypes were identified for Cyt b, which suggests high haplotype nucleotide diversities. Phylogeographic and population structure analyses using both DNA markers suggested that the wild giant spiny frog can be divided into four distinct major clades, i.e., Northern Vietnam, Western China, Central China, and Eastern China. The clades with significant genetic divergence are reproductively isolated, as evidenced by a high number of private alleles and strong incidence of failed amplification in microsatellite loci. Our research, coupled with other studies, suggests that Q. spinosa might be a species complex within which no detectable morphological variation has been revealed. The four phylogenetic clades and some subclades with distinct geographical distribution should be regarded as independent management units for conservation purposes.
基金supported by the Project of Tackling Key Problems of Science and Technology of Heilongjiang Province, China (GB06B205-3)Program of Wildlife Conservation and Breeding of State Forestry Administration of China (2008)Special Fund for Postgraduate Dissertation of Northeast Forestry University (2009)
文摘Commercial farming of anuran species that are declining in the wild raises a need to discriminate wild from farmed frogs.We hypothesized wild frogs might have extended hindlimbs due to greater frequency or intensity of jumping relative to farmed frogs,highlighting a morphometric approach to discrimination of wild from farmed frogs using hindlimb length.In the present study,Dybowski's frog(Rana dybowskii) was used to test this hypothesis.We measured body mass(Mb) and hindlimb length(Lh) of 2-year old farmed frogs and wild frogs aged 2 to 5 years.Dybowski's frog demonstrated significant dimorphism in Mb and Lh.Mb was significantly greater among farmed 2-year old frogs in both sexes(p=0.000),while only among females was Lh significantly greater for wild frogs(p=0.000).Lh/Mb was used as an index for origin discrimination to eliminate the influence of Mb due to variation of husbandry conditions among farms.Mean Lh/Mb for farmed frogs was significantly lower than for wild frogs(p=0.000) in the 2-year old age class.Discrimination correctly classified 84.4% of farmed and 96.3% of wild male frogs.Among females,92.9% of farmed frogs and 90.1% wild frogs were correctly classified.The overall correctness of classification was 92.1% and 90.8% for males and females,respectively.However,Lh/Mb revealed variation with age,resulting in reduced discriminative power for frogs ≥3 years old.We introduced a coefficient Ce to adjust the Lh/Mb of frogs ≥3 years to the level equivalent to 2-year frogs.The adjustment achieved 89.5% for overall correctness of origin for wild males and 92.4% for wild females ≥3 years old.These results show that Lh/Mb is an effective index to discriminate wild from farmed Dybowski's frog.Since the physical demands of jumping are common among anurans,this index is also potentially applicable to other anuran species.
基金supported by Open Fund of Key Laboratory of Bio-Macromolecular Evolution(No.2006B001)Foundation for outstanding academic team of Zoological Biology in Anhui Normal UniversitySpecial Foundation for Provincial Key Laboratories of Biotic Environment and Ecological Safety and Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province
文摘Changes in distribution density,morphology and secretory content of endocrine cells in the gastro-entero-pancreatic system of black-spotted frogs Rana nigromaculata before and after fasting were investigated using immunohistochemistry and antisera to six gut hormones.Six types of endocrine cells were detected in the digestive tract of Rana nigromaculata,including 5-hydroxytryptamine(5-HT),gastrin(GAS),somatostatin(SOM),glucagon(GLU),pancreatic polypeptide(PP)and vasoactive intestinal polypeptide(VIP)cells.After fasting,the density of 5-HT cells in the esophagus,cardia and fundus,GAS cells in the fundus and pylorus,PP cells in the fundus decreased significantly(P<0.01),while SOM cells in the cardia,GLU cells in the rectum increased significantly(P<0.01).The cytoplasmic processes of 5-HT cells became shorter or not detectable.The secretory content of GAS cells reduced in the cardia.The positive immunostaining reaction in the perinuclear region of SOM cells in the cardia,fundus and pylorus became weaker,while the staining intensity in the periphery of these cells became stronger.VIP cells were not detectable in the whole digestive tract after fasting.Five types of endocrine cells were found in the pancreas of Rana nigromaculata,including 5-HT,GAS,SOM,GLU and PP cells.After fasting,the density of 5-HT cells decreased slightly(P>0.05),while SOM,GAS,GLU and PP cells increased significantly(P<0.01).Furthermore,the secretory content of GLU cells increased significantly.Considering their functionalities,our results indicate that the changes of GEP endocrine cells in Rana nigromaculata responded adaptively to starvation-induced stress.
基金Supported by National Natural Science Foundation of China(Grant No.51675124)
文摘Pneumatic muscles with similar characteristics to biological muscles have been widely used in robots, and thus are promising drivers for frog inspired robots. How- ever, the application and nonlinearity of the pneumatic system limit the advance. On the basis of the swimming mechanism of the frog, a frog-inspired robot based on pneumatic muscles is developed. To realize the indepen- dent tasks by the robot, a pneumatic system with internal chambers, micro air pump, and valves is implemented. The micro pump is used to maintain the pressure difference between the source and exhaust chambers. The pneumatic muscles are controlled by high-speed switch valves which can reduce the robot cost, volume, and mass. A dynamic model of the pneumatic system is established for the sim- ulation to estimate the system, including the chamber, muscle, and pneumatic circuit models. The robot design is verified by the robot swimming experiments and the dynamic model is verified through the experiments and simulations of the pneumatic system. The simulation results are compared to analyze the functions of the source pressure, internal volume of the muscle, and circuit flow rate which is proved the main factor that limits the response of muscle pressure. The proposed research provides the application of the pneumatic muscles in the frog inspired robot and the pneumatic model to study muscle controller.