Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three t...Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-KB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyi-D- aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.展开更多
目的运用多层螺旋CT高分辨扫描和多平面重建技术对健康人群和慢性鼻窦炎人群各型额气房的出现率进行研究。方法选择本院就诊的慢性鼻窦炎患者173例和同期既往无鼻窦炎病史及CT检查显示无鼻窦病变患者94例,采用美国GE公司8排螺旋CT扫描...目的运用多层螺旋CT高分辨扫描和多平面重建技术对健康人群和慢性鼻窦炎人群各型额气房的出现率进行研究。方法选择本院就诊的慢性鼻窦炎患者173例和同期既往无鼻窦炎病史及CT检查显示无鼻窦病变患者94例,采用美国GE公司8排螺旋CT扫描机进行横断面扫描,将扫描数据传输至GE Advantage Windows 4.0后台工作站,应用MPR工具在同一屏幕上重建成连续的冠状位、矢状位图像,并对各型额气房进行辨认和统计学分析。结果健康组病例与慢性鼻窦炎组病例额气房观测出现率之间有统计学意义(P<0.05)。慢性鼻窦炎组中无额窦病变组病例与有额窦病变组病例额气房观测出现率之间有统计学意义(P<0.05)。结论位于额隐窝引流通道中的额气房对于额隐窝的阻塞及额窦炎症的发生所起的作用是非常显著的。展开更多
基金funded by the General Project of Medical Technology of Military during the "12~(th) Five-Year Plan"Period, No. CWS11J00
文摘Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-KB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyi-D- aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
文摘目的运用多层螺旋CT高分辨扫描和多平面重建技术对健康人群和慢性鼻窦炎人群各型额气房的出现率进行研究。方法选择本院就诊的慢性鼻窦炎患者173例和同期既往无鼻窦炎病史及CT检查显示无鼻窦病变患者94例,采用美国GE公司8排螺旋CT扫描机进行横断面扫描,将扫描数据传输至GE Advantage Windows 4.0后台工作站,应用MPR工具在同一屏幕上重建成连续的冠状位、矢状位图像,并对各型额气房进行辨认和统计学分析。结果健康组病例与慢性鼻窦炎组病例额气房观测出现率之间有统计学意义(P<0.05)。慢性鼻窦炎组中无额窦病变组病例与有额窦病变组病例额气房观测出现率之间有统计学意义(P<0.05)。结论位于额隐窝引流通道中的额气房对于额隐窝的阻塞及额窦炎症的发生所起的作用是非常显著的。
文摘目的通过分析鼻内镜手术后复发额窦炎患者的电子计算机断层显像(computed tomography,CT),探究额隐窝气房残留情况以及再次手术后的治疗效果。方法回顾性分析2017年1月至2019年12月于北京大学人民医院耳鼻咽喉科住院行手术治疗的慢性鼻窦炎术后复发额窦炎的患者64例,统计患者术前及术后1年的鼻部症状视觉模拟量表(Visual Analog Scales,VAS)评分。根据国际额隐窝气房分型(The International Frontal Sinus Anatomy Classification,IFAC)的标准对患者鼻窦CT进行分析,对各类额隐窝气房的残留情况进行统计,同时统计各额窦的Lund-Mackay评分(LM值),使用SPSS 27.0软件对患者的一般信息及额隐窝气房残留情况与额窦LM值的关系进行统计学分析,分析患者术前和术后VAS评分变化有无统计学意义。结果共分析额窦121侧,各种额隐窝气房均有残留,气房残留情况为:鼻丘气房共108侧(89.3%)、鼻丘上气房共11侧(9.1%)、鼻丘上额气房共19侧(15.7%)、筛泡上气房共20侧(16.5%)、筛泡上额气房共13侧(10.7%)、眶上筛房共40侧(33.1%)、额窦间隔气房共49侧(40.5%)、1型额窦间隔气房共15侧(12.4%)、2型额窦间隔气房共34侧(28.1%),其中额窦间隔气房和眶上筛房相对更容易残留。术后1年随访人数为54例,患者术前及术后1年鼻部症状VAS评分平均值分别为6.6±1.4和2.8±1.4。共7例行DrafⅢ型手术,术前及术后1年鼻部症状VAS评分平均值分别为7.6±1.1和3.9±0.9。统计学分析显示,先前的手术次数(P=0.016,OR=2.639)与额窦LM值增长相关,患者术前及术后VAS评分差异有统计学意义(t=19.656,P<0.01)。结论复发的鼻窦炎患者中部分额隐窝气房残留比例较高,这可能是导致术后复发的危险因素,在手术中应彻底清除额隐窝气房,通畅引流,预防复发。
