Analysis of short fruiting branch gene and Marker-assisted selection with SNP linked to its trait in upland cotton

Background： With the rapid development of genomics, many functional genes have been targeted. Molecular marker assisted selection can accelerate the breeding process by linking selection to functional genes. Methods： In a study of upland cotton （Gossypium hirsutum L.）, the F2 segregated population was constructed by crossing X1570 （short branches） with Ekangmian 13 （long branches） to identify the short fruiting branch gene and marker assisted selection with SNP（Single Nucleotide Polymorphisms, SNP） linked to its trait. Result： The result demonstrated that linked SSR marker BNL3232 was screened by BSA（Bulked segregant analysis, BSA） method; one SNP locus was found, which was totally separated from the fruiting branches trait in upland cotton. Conclusion： It was verified that this SNP marker could be used for molecular assisted selection of cotton architecture

The Correlation between Different Fruiting Branch Diameters of Actinidia chinensis and Fruit Quality Change in Post-ripening Period

he research aimed to explore the correlation between fruiting branch diameter of A. chinensis and fruit quality change during the post-ripening period and the mechanism,which could provide theoretical basis for making scientific management measures during A. chinensis fruit post-ripening period. In this paper,correlation analysis between fruiting branch diameter of A. chinensis and fruit quality change during post-ripening period during 2013- 2015 was conducted. Results were as below:(i) correlation coefficient analysis showed that fruiting branch diameter was highly positive correlation with number of fruit ventricle,significantly positive correlation with single fruit weight when picking and the indexes after the fruit ripening single(single fruit weight,loss rate of single fruit weight,longitudinal diameter of fruit and width of fruit core),lowly positive correlation with sugar content of fruit juice,and positive correlation with thick transverse diameter of fruit after ripening.(ii) Standard errors of regression analysis between fruiting branch diameter and single fruit weight,longitudinal diameter of fruit,wide transverse diameter of fruit,thick transverse diameter of fruit,fruit core length and sugar content of fruit juice were 12. 4248,4. 2731,2. 6452,2. 0260,0. 1337 and 1. 0035 respectively; significance F values of variance analysis were 0. 0743,0. 0658,0. 1950,0. 5733,0. 2600 and 0. 1517 respectively. It showed that fruiting branch diameter had a significant difference with thick transverse diameter of fruit and insignificant differences with other indicators; quadratic curve regression equation and linear regression equation of residual curve,observed value curve,forecast value curve and normal probability curve of were analyzed,and the results were all in line with the law of quadratic curve.(iii)The larger the fruiting branch diameter,the greater the indexes after post-ripening(single fruit weight,longitudinal diameter of fruit,wide transverse diameter of fruit,thick transverse diameter of fruit and sugar content of fruit juice),the better the fruit quality. It was clear that fruiting branch diameter could be as one of important bases of fruit quality change during post-ripening period. Through investigating fruiting branch diameter and the regression equation,it could predict single fruit weight,longitudinal and transverse diameters of fruit,and sugar content of fruit juice index after the ripening.

Influence of Anthesis Date and Boll Branch Position on Qualities and Super-molecular Structure of Cotton Fiber

The influences of the anthesis date and the fruiting branch positions on parameters of cotton fiber quality and super-molecular structure were studied. Fiber quality parameters, such as cotton fiber length, micronair, maturity and fiber strength deteriorated with postponing the anthesis date and decreasing temperature. When anthesis happened on the same date, the fruiting branch positions affected the fiber quality parameters, and the fiber quality parameters of the lower portion turned out to be higher than that of the upper portion, although the difference was insignificant. The super-molecular structure of the lower portion turned out to be superior to that of the upper portion at the early anthesis date and high temperature, which is in accordance with what was mentioned above, but the fruiting branch positions did not affect the impact of the anthesis date on the super-molecular structure.