Liaoqiao aqueous extract inhibits B16 melanoma growth involving MAPKs/Nrf2/HO-1 mediated anti-oxidation and anti-inflammation

Aim Forsythia suspensa （Thunb.） Vahl, Lianqiao in Chinese, is one of the most fundamental herbs in traditional Chinese medicine （TCM） with heat-clearing and detoxicating properties. In this study, we aimed to study the antitumor activity of Lianqiao aqueous extract against melanoma using cancer cell line-based in vitro and mouse allografl tumor in vivo models. Furthermore, we also investigated the underlying molecular mechanisms, par- ticularly the involvement of anti-inflammation and anti-oxidation properties in its antitumor activity. Methods The proliferation of cancer cells was measured by MTT assay. The transplanted B16-F10 melanoma in C57BL/6 mice were established and used for the evaluation of in vivo antitumor effect of LQ. Tumor growth was monitored twice a week. Ki67 and CD31 were used to detect cancer cell proliferation and angiogenesis in tumor, respectively. The anti-oxidative property of LQ was determined by measuring the levels of ROS, MDA and GSH. The anti-inflamma- tory effect of LQ was evaluated by measuring TNF-α and IL-6 using ELISA kits. Other protein expression was deter- mined by Western Blot. Results LQ strongly inhibited the growth of B16-F10 cells in vitro and the tumor growth in vivo. The survival time of tumor-bearing mice was significantly prolonged by LQ. LQ inhibited cancer cell prolif- eration and angiogenesis in tumor as evidenced by decreased expressions of Ki67 and CD31. Levels of ROS, MDA TNF-α and IL-6 decreased, while GSH increased in LQ treatment group, indicating a strong anti-oxidative and an- ti-inflammatory activity of LQ. The expression of antioxidant proteins Nff-2 and HO-1, tumor suppressors P53 and p-PTEN, and the MAPK pathways in tumor tissues were upregulated by LQ treatment. Conclusions LQ exhibited strong antitumor activity against B16-F10 murine melanoma both in vitro and in vivo. The antitumor effect of LQ in- volved the decreased oxidative stress and inflammation in tumor, which is closely related to the heat-clearing and detoxicating properties of LQ.

Resveratrol combats chronic diseases through enhancing mitochondrial quality

Resveratrol(RSV),as a functional food component extracted from natural plants,has been widely studied and recognized in preventing and treating various diseases,with major mechanisms including executing anti-inflammation and anti-oxidation functions,and improving mitochondrial quality.Chronic diseases as non-communicable diseases are mainly caused by multiple factors,such as physiological decline and dysfunction in the body,and have become a significant challenge on public health worldwide.It is worth noting that chronic diseases such as Alzheimer's disease(AD),Parkinson's disease(PD),muscle atrophy,cardiovascular disease,obesity,and cancer are accompanied by abnormal mitochondrial function.Therefore,targeted regulation of mitochondria may be a meaningful way to prevent and treat chronic diseases.Increasing evidence has confirmed that RSV is actively involved in regulating mitochondria,and it has become an essential consideration to prevent and treat chronic diseases through targeting mitochondria and improving corresponding functions.In this article,current studies on RSV to optimize mitochondrial quality for preventing and alleviating chronic disease are systematically summarized,which can provide a theoretical reference for the development of functional foods or drugs to combat chronic diseases.

Antioxidant and anti-inflammatory roles of tea polyphenols in inflammatory bowel diseases

Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of tea.High intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and cancer.This review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox homeostasis.Tea polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD development.Accumulating pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory colitis.Therefore,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.

Food Functionality of Popular and Commonly Consumed Indigenous Vegetables and Fruits from Bangladesh

Functional food can be either natural or processed. Food contains known biologically active compounds, which provides clinically proven and documented health benefit. So far, no database is available in Bangladesh regarding the functionality of the indigenous foods. As a primary step, this study had been conducted with an aim to identify and characterize functionality of the indigenous foods of Bangladesh. Eight fruits and vegetables, which are very popular for their taste, price and easy availability, have been selected for in vitro screening of their functionality. High levels of fat and sugar binding capacity were observed in Pineapple (500% and 270.891% respectively). High nuplli mortality rate was found in cytotoxicity test with Pomelo extract (100%), which also contained high level of vitamin C (155 mg/100g). Pomelo contained the highest level of antioxidant among all these foods (0.674 nMol/100μl/mg protein). Red amaranth contained highest amount of protein (4.31 g/100g). In stem amaranth, Iron level was highest (1855.67 mg/kg). Total phenolics content of Water spinach and Stem amaranth were found higher than the other samples (59.339 and 55.728 mg GAE/g respectively). All these experiments showed these vegetables and fruits have high health impacts and can be act as potential functional foods.

Metallothionein:An overview

Metallothioneins （MTs） were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal levels of MTs since they are involved in metalloregulatory processes that include cell growth and multiplication. In an effort to understand the biology of this intriguing tumor, various biomarkers such as oncogenes, p53 tumor suppressor gene, war 1 protein, proliferating cell nuclear antigen, telomerase, microsatellite markers and cytogenetic changes have been examined. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is MT. Immunohistochemical detection of MT proteins in cold acetone-fixed paraffin embedded liver sections was performed by the streptavidin-avidin-biotin immunoperoxidase complex method.

(＋)-Borneol is neuroprotective against permanent cerebral ischemia in rats by suppressing production of proinflammatory cytokines

Stroke is one of the leading causes of disability and death globally.It occurs when a major artery is occluded in the brain and leads to death of cells within the injured tissue.（＋）-Borneol,a simple bicyclic monoterpene extracted from traditional Chinese medicine,is widely used in various types of diseases.However,no study has proved the effects of（＋）-borneol on functional recovery from permanent ischemic stroke and the mechanism is still unknown.Here,we report that in the rat model of permanent cerebral ischemia,we found that（＋）-borneol（1.0 mg/kg） significantly ameliorated infarct size and neurological scores via reducing the expression of inducible nitric oxide synthase（iNOS）and tumor necrosis factor-alpha（TNF-α） in a dose dependent manner.Notably,（＋）-borneol showed long-term effects on the improvement of sensorimotor functions in the photothrombotic model of stroke,which decreased the number of foot faults in the grid-walking task and forelimb asymmetry scores in the cylinder task,at least in part through reducing loss of dendritic spines in the length,brunch number and density.These findings suggest that（＋）-borneol could serve as a therapeutic target for ischemic stroke.

Protective effect of Cordyceps sinensis extract on rat brain microvascular endothelial cells injured by oxygen-glucose deprivation

Objective:To investigate the protective effect of Cordyceps sinensis extract (CSE)on injury of primary cultured rat brain microvascular endothelial cells (rBMECs) induced by oxygen-glucose deprivation (OGD).Methods:We isolated and cultured primary rBMECs in order to establish an in vitro OGD model.Cellular activity was detected using a cell counting kit to determine the appropriate dosage.The rBMECs were divided into control,model,low-,mid-,and high-dose (5,10,20 μg.mL-1) CSE groups under OGD for 6 hours.CSE was dissolved in cell culture medium to the appropriate concentration,passed through a 0.22 μm sterile filter,and administered for 12 hours before and during OGD.Cellular morphology was observed under a microscope.Lactate dehydrogenase level in cultural supernatant,superoxide dismutase activity,and the content of nitric oxide and malondialdehyde in cells were tested by colorimetric methods.Levels of tumor necrosis factor-α and interleukin-1 beta in cells were determined by enzyme-linked immunosorbent assay.Results:After 12-hour administration of CSE at the concentration of 5,10,20 iμg.mL-1 before and during OGD,compared with the model group,the CSE groups obviously alleviated the damage of rBMECs induced by OGD,inhibited the apoptosis and the necrosis of the cells,and improved cellular morphology of rBMECs.Additionally,compared with the model group,CSE also restrained lactate dehydrogenase leakage in hypoxic cells (P <.01),significantly increased superoxide dismutase activity (P <.05),and reduced the levels of nitric oxide,malondialdehyde,tumor necrosis factor-α,and interleukin-1 beta (P <.05).Conclusion:C.sinensis extract plays a significant role in protecting injured primary cultured rBMECs induced by OGD.The mechanism may be related with the increase of cellular antioxidative capacity and anti-inflammatory effect.

Forsythiae Fructus inhibits melanoma growth through activating MAPKs/Nrf2/HO-1 signaling and modulating glycerophospholipid metabolism

OBJECTIVE Forsythiae Fructus(Lianqiao)is a typical heat-clearing and detoxicating traditional Chinese medicine(TCM)herb,which has been traditionally used for treating cancer according to TCM theory.However,the underlying mechanism has not been fully explained.METHODS In this study,we investigated the antitumor effect of Forsythiae Fructus aqueous extract(FAE)on B16-F10 melanoma.RESULTS FAE strongly inhibited the tumor growth and metastasis formation in B16-F10 melanoma transplanted mice.The survival time of tumor-bearing mice was also significantly prolonged by FAE.The levels of ROS,MDA,TNF-αand IL-6 decreased,while GSH increased in the FAE treatment group,indicating FAE possesses strong anti-oxidative and anti-inflammatory activity.Western blotting analysis demonstrated that antioxidant proteins Nrf2 and HO-1,tumor suppressors P53 and p-PTEN,and the MAPK pathways in tumor tissues were upregulated by FAE treatment.Serum metabolomics analysis further uncovered that 17 metabolites mostly involving in glycerophospholipid metabolism were correlated with the antitumor effect of FAE.Notably,several lysophosphatidylcholines(LysoPCs)significantly decreased in tumor model group,while FAE treatment restored the changes of these phospholipids to about normal condition.LysoPC acyltransferase 1(LPCAT1)and autotaxin(ATX)highly expressed in melanoma and markedly downregulated by FAE were believed to be responsible for this modulation.CONCLUSION FAE exhibites strong antitumor activity against B16-F10 melanoma through activating MAPKs/Nrf2/HO-1 mediated anti-oxidation and anti-inflammation and modulating glycerophospholipid metabolism via downregulating LPCAT1 and ATX.Besides,it is suggested that serum LysoPCs could be potential biomarkers for the diagnosis and prognosis of melanoma.

Vitamin C supplementation in patients on maintenance dialysis

As one of the most important water-soluble non-enzymatic antioxidants,vitamin C consists of ascorbic acid and its oxidized form,dehydroascorbic acid.Maintenance hemodialysis(MHD)patients have a generally lower plasma vitamin C level compared with general population.Moreover,dialysis patients also exhibit a low plasma vitamin C level,which is largely related with increased inflammation,refractory anemia and oxidative stress.In this review,we described,in great detail,the vitamin C deficiency in MHD patients and its effects on anti-oxidation,anti-inflammation,pro-oxidation and secondary hyperparathyroidism.In addition,we described the possible potential value of vitamin C in anemia,and the side effects of over-doses of vitamin C supplementation in this particular population.In summary,MHD patients may benefit from vitamin C administration.However,further research should be carried out to confirm its potential beneficial effects,optimal dosage and side effects from vitamin C supplementation.

Functional biomaterials for comprehensive periodontitis therapy

Periodontitis is an inflammatory disease caused by bacterial infection directly, and the dysregulation of host immune-inflammatory response finally destroys periodontal tissues. Current treatment strategies for periodontitis mainly involve mechanical scaling/root planing(SRP), surgical procedures,and systemic or localized delivery of antimicrobial agents. However, SRP or surgical treatment alone has unsatisfactory long-term effects and is easy to relapse. In addition, the existing drugs for local periodontal therapy do not stay in the periodontal pocket long enough and have difficulties in maintaining a steady, effective concentration to obtain a therapeutic effect, and continuous administration always causes drug resistance. Many recent studies have shown that adding bio-functional materials and drug delivery systems upregulates the therapeutic effectiveness of periodontitis. This review focuses on the role of biomaterials in periodontitis treatment and presents an overview of antibacterial therapy, host modulatory therapy, periodontal regeneration, and multifunctional regulation of periodontitis therapy. Biomaterials provide advanced approaches for periodontal therapy, and it is foreseeable that further understanding and applications of biomaterials will promote the development of periodontal therapy.

Stachytarpheta cayennensis Aqueous Extract,a New Bioreactor towards Silver Nanoparticles for Biomedical Applications

This study reports the preparation and characterization of silver nanoparticles synthesized by the mediation of the plant weed Stachytarpheta cayennensis through solution method. Ultraviolet visible spectroscopy (UV-Vis) determines the presence of nanoparticles in the solution. Infrared spectroscopy (IR) proves organic molecules at the particles interface. Powder X-ray diffraction (PXRD) provides phase composition and crystallinity. Shape was showed by scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX) demonstrated the elemental mapping of the silver nanoparticles. Hydrogen peroxide scavenging and phosphomolybdenum antioxidant assays, egg albumin denaturation anti-inflammation study, and the formation mechanism complete the study. The particles have been found composed of pure silver Ag and silver chloride AgCl nanocrystallites. The average crystallite sizes were found to be 13 nm and 20 nm for Ag and AgCl respectively. A Rietveld refinement based XRD pattern data followed by Williamson-Hall plot allows a size and strain analysis. Based on SEM, spherical agglomerates materials were formed and EDX proved the presence of Cl- ions. The reaction formation mechanism of Ag and AgCl is proposed to be simultaneous and competitive. The silver nanoparticles moderately inhibit the denaturation of egg albumin and exhibit antioxidant action;hence, the nanoparticles could be considered as a potential source for biomedical applications.

Protective role of fruits of Rosa odorata var. gigantea against WIRSinduced gastric mucosal injury in rats by modulating pathway related to inflammation, oxidative stress and apoptosis

Objective: Rosa odorata var. gigantea is a popular medicinal plant. Some studies have demonstrated that ethanolic extract of the fruits of R. odorata var. gigantea(FOE) has gastroprotective properties. The aim of this study was to investigate the gastroprotective activity of FOE on water immersion restrained stress(WIRS)-induced gastric mucosal injury in a rat model and elucidate the possible molecular mechanisms involved.Methods: A rat stress ulcer model was established in this study using WIRS. After rats were treated with FOE orally for 7 d, the effect of FOE treatment was analyzed by hematoxylin and eosin(H&E) staining, and the changes of inflammatory factors, oxidative stress factors, and gastric-specific regulatory factors and pepsin in the blood and gastric tissues of rats were examined by ELISA assay. Molecular mechanism of FOE was investigated by immunohistochemical assay and Western blot.Results: Compared with the WIRS group, FOE could diminish both the macroscopic and microscopic pathological morphology of gastric mucosa. FOE significantly preserved the antioxidants glutathione peroxidase(GSH-PX), superoxide dismutase(SOD) and catalase(CAT) contents;anti-inflammatory cytokines interleukin-10(IL-10) and prostaglandin E2(PGE2) levels as well as regulatory factors tumor necrosis factor-a(TGF-a) and somatostatin(SS) contents, while decreasing malondialdehyde(MDA), nitric oxide synthase(i NOS), tumor necrosis factor(TNF-a), interleukin-1β(IL-1β), interleukin-6(IL-6), gastrin(GAS)and endothelin(ET) levels. Moreover, FOE distinctly upregulated the expression of Nrf2, HO-1, Bcl2 and proliferating cell nuclear antigen(PCNA). In addition, FOE activated the expression of p-EGFR and downregulated the expression of NF-ΚB, Bax, Cleaved-caspase-3, Cyto-C and Cleaved-PARP1, thus promoting gastric mucosal cell survival.Conclusion: The current work demonstrated that FOE exerted a gastroprotective activity against gastric mucosal injury induced by WIRS. The underlying mechanism might be associated with the improvement of anti-inflammatory, anti-oxidation and anti-apoptosis systems.

Crocodile blood supplementation protect svascular function in diabetic mice

Cardiovascular disease is a major cause of mortality in diabetic patients due to the heightened oxidative stress and pro-inflammatory state in vascular tissues.Effective approaches targeting cardiovascular health for diabetic patients are urgently needed.Crocodile blood,an emerging dietary supplement,was suggested to have anti-oxidative and anti-inflammatory effects in vitro,which have yet to be proven in animal models.This study thereby aimed to evaluate whether crocodile blood can protect vascular function in diabetic mice against oxidation and inflammation.Diabetic db/db mice and their counterparts db/m+mice were treated daily with crocodile blood soluble fraction(CBSF)or vehicle via oral gavage for 4 weeks before their aortae were harvested for endothelium-dependent relaxation(EDR)quantification using wire myograph,which is a well-established functional study for vascular function indication.Organ culture experiments culturing mouse aortae from C57BL/6 J mice with or without IL-1βand CBSF were done to evaluate the direct effect of CBSF on endothelial function.Reactive oxygen species(ROS)levels in mouse aortae were assessed by dihydroethidium(DHE)staining with inflammatory markers in endothelial cells quantified by quantitative polymerase chain reaction(qPCR).CBSF significantly improved deteriorated EDR in db/db diabetic mice through both diet supplementation and direct culture,with suppression of ROS level in mouse aortae.CBSF also maintained EDR and reduced ROS levels in mouse aortae against the presence of pro-inflammatory IL-1β.Under the pro-inflammatory state induced by IL-1β,gene expressions of inflammatory cytokines were downregulated,while the protective transcripts UCP2 and SIRT6 were upregulated in endothelial cells.Our study suggests a novel beneficial effect of crocodile blood on vascular function in diabetic mice and that supplementation of diet with crocodile blood may act as a complementary approach to protect against vascular diseases through anti-oxidation and anti-inflammation in diabetic patients.

Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways

The present study was designed to evaluate protective activity of an ethanol extract of the stems of Schisandra chinensis(SCE) and explore its possible molecular mechanisms on acetaminophen(APAP) induced hepatotoxicity in a mouse model. The results of HPLC analysis showed that the main components of SCE included schisandrol A, schisandrol B, deoxyschisandrin, schisan--drin B, and schisandrin C and their contents were 5.83, 7.11, 2.13, 4.86, 0.42 mg·g^(-1), respectively. SCE extract was given for 7 con--secutive days before a single hepatotoxic dose of APAP(250 mg·kg^(-1)) was injected to mice. Our results showed that SCE pretreatment ameliorated liver dysfunction and oxidative stress, which was evidenced by significant decreases in aspartate transaminase(AST), alanine aminotransferase(ALT), malondialdehyde(MDA) contents and elevations in reduced glutathione(GSH) and superoxide dismutase(SOD) levels. These findings were associated with the result that the SCE pretreatment significantly decreased expression levels of 4-hydroxynonenal(4-HNE) and 3-nitrotyrosine(3-NT). SCE also significantly decreased the expression levels of Bax, mitogen-activated protein kinase(MAPK), and cleaved caspase-3 by APAP exposure. Furthermore, supplementation with SCE suppressed the expression levels of inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2), suggesting alleviation of inflammatory response. In summary, these findings from the present study clearly demonstrated that SCE exerted significant alleviation in APAP-induced oxidative stress, inflammation and apoptosis mainly via regulating MAPK and caspase-3 signaling pathways.

Neuroprotective Effects of Molecular Hydrogen:A Critical Review

Molecular hydrogen(H_(2))is a physiologically inert gas.However,during the last 10 years,increasing evidence has revealed its biological functions under pathological conditions.More specifically,H_(2) has protective effects against a variety of diseases,particularly nervous system disorders,which include ischemia/reperfusion injury,traumatic injury,subarachnoid hemorrhage,neuropathic pain,neurodegenerative diseases,cognitive dysfunction induced by surgery and anesthesia,anxiety,and depression.In addition,H_(2) plays protective roles mainly through anti-oxidation,anti-inflammation,antiapoptosis,the regulation of autophagy,and preservation of mitochondrial function and the blood-brain barrier.Further,H_(2) is easy to use and has neuroprotective effects with no major side-effects,indicating that H_(2) administration is a potential therapeutic strategy in clinical settings.Here we summarize the H_(2) donors and their pharmacokinetics.Meanwhile,we review the effectiveness and safety of H_(2) in the treatment of various nervous system diseasesbased on preclinical and clinical studies,leading to the conclusion that H_(2) can be a simple and effective clinical therapy for CNS diseases such as ischemia-reperfusion brain injury,Parkinson's disease,and diseases characterized by cognitive dysfunction.The potential mechanisms involved in the neuroprotective effect of H_(2) are also analyzed.

Studies on the structure-activity relationship of caffeate derivatives as neuroprotective agents

In the present study,novel ester derivatives of CAPE were designed and synthesized as neuroprotective agents.The anti-inflammatory and antioxidant activities of these compounds were evaluated at the cellular level,while the blood-brain barrier(BBB)permeability was predicted by parallel artificial membrane permeability assay(PAMPA).The results revealed that phenolic hydroxyl groups and double bonds in the structure of CAPE had important effects on neuroprotective activities.Accordingly,a preliminary structure-activity relationship was summarized in this paper.In addition,we observed a significant improvement on BBB permeability.These results provided important references for the structural modification and optimization of CAPE in the future.

Mechanism progress of acupuncture and moxibustion for chronic prostatitis

The effect of acupuncture and moxibustion in the treatment of chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS) is definite,and progress has been made in the studies of its functional mechanism.This paper discusses the related studies on the mechanism of acupuncture and moxibustion in the treatment of CP/CPPS from five aspects:regulating immunity,anti-oxidative stress,regulating neural functions,improving urodynamics,and regulating blood circulation.It is found that acupuncture and moxibustion can decrease the level of pro-inflammatory factors,increase the level of anti-inflammatory factors,and regulate the level of inflammatory regulatory factors,thus to regulate immunity.Acupuncture and moxibustion can improve the body’s ability of anti-oxidative stress,regulate the balance state of oxidation and anti-oxidation,thus to relieve tissue damage,and regulate neural functions by modulating neurotransmitters and autonomic nerves.Acupuncture and moxibustion can improve urodynamics and relieve abnormal urination symptoms by regulating the contraction of pelvic floor muscles,can also promote blood circulation to treat the pelvic congestion.It is expected that this paper can provide reference for the clinical application and research of acupuncture and moxibustion for CP/CPPS.

The inhibitory activities of Urtica fissa to BPH

In the present study, the BPH inhibitory activities of Urtica fissa were systematically investigated. Firstly, inhibitory activities of 5α reductase of the alcoholic extracts from different parts(root, stem, leaf, flower and fruit) were evaluated. The results indicated that the root possessed the most significant action. Subsequently, U. fissa root(UFR) was subjected to further pharmacological evaluation using the benign prostate hyperplasia(BPH) model rats induced by testosterone propionate. The results revealed that UFR could significantly decrease the prostate index, alter the hyperplasia tissue morphology, suppress the prostatic growth factors of VEGF, EGF, bF GF and KGF, decrease the inflammation factor levels of TNF-α, IL-1β and IL-6, improve the activities of GSH-Px, CAT and SOD and decrease the MDA level in the prostate of the model rats. Moreover, UFR also significantly suppressed the hormone levels of testosterone and dihydrotestosterone. These results indicated that the possible BPH inhibitory mechanisms of UFR were growth factor suppression, hormone level modulation, anti-inflammation, and anti-oxidative stress.

Flavonoids from rape bee pollen on the clinical effects of antioxidant activity

Background Flavonoids from fruits, vegetables and plants have been widely studied on their improving lipidemia, anti-inflammation, anti-platelet aggregation and anti-oxidative activities. However, t know if flavonoids from rape bee pollen have the same effects in patients with dyslipidemia （DL）, effects of we don＇ type 2 diabetes mellitus（T2DM）, and cerebral infarction（CI）. Methods The study involved with patients selected and divided into 4 groups, 30 cases of DL, of T2DM, of CI and health control （HC） for each. Forty mg flavonoids from rape bee pollen administrated orally twice a day for two months. Blood sugar （BG）, total cholesterol （TC）, triglyceride （TG）, high density lipoprotein-cholesterol （HDL-C）, low white blood-cell counts （WBC）, platelet counts （PLT）, high-sens malondialdehyde （MDA） were analyzed in four groups before and after density lipoprotein-cholesterol （LDL-C）, itivity C-reactive protein （hsCRP） and taking flavonoids for two months. Results （1） Basic levels of TC, BG, WBC, PLT and hsCRP in groups of T2DM, CI, and TG and MDA in groups of DL, PLT, T2DM and CI were significantly higher than those in HC （P 〈 0.05-0.001）. （2） The levels of hsCRP and MDA were significantly decreased, flavonoids for two months （P 〈 0.05-0.001） in group of while HDL-C was remarkably increased DL, T2MD, and CL These indexes were not the group of HC after taking flavonoids （P 〉 0.05）. Conclusions The results could indieat disturbance, inflammation, and high oxidative stress in patients with dyslipidemisa, type 2 diabet TG, WBC, after taking different in e metabolic es mellitus, and cerebral infarction. The flavonoids from rape bee pollen may have effects of antioxidant activity, improving lipids, and anti-inflammation on these patients.