SFC placement and dynamic resource allocation based on VNF performance-resource function and service requirement in cloud-edge environment

With the continuous development of network func-tions virtualization(NFV)and software-defined networking(SDN)technologies and the explosive growth of network traffic,the requirement for computing resources in the network has risen sharply.Due to the high cost of edge computing resources,coordinating the cloud and edge computing resources to improve the utilization efficiency of edge computing resources is still a considerable challenge.In this paper,we focus on optimiz-ing the placement of network services in cloud-edge environ-ments to maximize the efficiency.It is first proved that,in cloud-edge environments,placing one service function chain(SFC)integrally in the cloud or at the edge can improve the utilization efficiency of edge resources.Then a virtual network function(VNF)performance-resource(P-R)function is proposed to repre-sent the relationship between the VNF instance computing per-formance and the allocated computing resource.To select the SFCs that are most suitable to deploy at the edge,a VNF place-ment and resource allocation model is built to configure each VNF with its particular P-R function.Moreover,a heuristic recur-sive algorithm is designed called the recursive algorithm for max edge throughput(RMET)to solve the model.Through simula-tions on two scenarios,it is verified that RMET can improve the utilization efficiency of edge computing resources.

FC-TBL教学模式在生物制品学课程中的实践研究

生物制品学是以现代生物技术为核心的一门新的独立学科,其研究成果在全球重大、流行性疾病的预防、治疗、诊断中发挥了不可忽视的作用。本研究以培养创新型生物技术人才为目标,在生物制品学课程中开展了以互联网+学习平台为基础的“自主初探+团队协作+师生探讨”相结合的以团队为基础的翻转课堂(flipped classroom and team-based learning,FC-TBL)教学模式,并从教学设计、教学资源平台建设、教学评价体系建立3个方面总结了该教学模式的实践经验,以期为相关教学研究提供参考。

基于网络药理学探究三七皂苷Ft1和Fc治疗血栓的潜在位点分析

为探讨三七中活性成分三七皂苷Ft1和Fc治疗血栓疾病的作用机制,通过Swiss Target Prediction、Super-pred、BindingDB数据库检索三七Ft1、Fc靶点,在OMIM、Gene Cards、Drug Bank数据库中检索与血栓疾病发生相关靶点,借助Venny网站和STRING数据库建立靶蛋白互作网络模型,使用Centiscape 2.2进行拓扑参数分析,并且对三七Ft1和Fc核心靶点进行GO、KEGG通路富集分析,最后进行分子对接验证。结果表明,三七皂苷Ft1、Fc分别有90和149个预测靶点,4303个与血栓疾病发生相关靶点,维恩图分析核心靶点分别为14和18个,其中三七Ft1与其核心靶点STAT3、VEGFA、HSP90AA1紧密结合,三七Fc与其核心靶点STAT3、VEGFA、CXCR4紧密结合。基于网络药理学,探讨三七皂苷Ft1和Fc治疗血栓多靶点和多途径的特性,为三七皂苷Ft1和Fc治疗血栓疾病的研究提供了新思路。

复方双黄连制剂对单核巨噬细胞(RAW264.7)Fc/C3b受体活性和分泌功能的影响

【目的】探究复方双黄连制剂对巨噬细胞吞噬能力和分泌功能的影响,为复方双黄连的深度开发及畜禽用药的合理选择提供科学依据。【方法】将金银花、黄芩、连翘、穿心莲干燥粉碎后分别制备浸膏,按照比例制备双黄连口服液(金银花∶黄芩∶连翘=1∶1∶2)、复方双黄连口服液(金银花∶黄芩∶连翘∶穿心莲=1∶1∶2∶2)及穿心莲口服液,调节pH为7.0,生药浓度为1 g/mL。3种药物作用于小鼠单核巨噬细胞RAW264.7,采用CCK-8法检测细胞活力,确定3种药物安全浓度,将药物最大安全浓度以二倍稀释法稀释为3个浓度;采用脂多糖(LPS)和氢化考的松琥珀酸钠(HCSS)作用于RAW264.7细胞模拟机体炎症和免疫抑制状态,3种药物作用于这2种状态的细胞和正常细胞,采用YC、EA玫瑰花环法及中性红吞噬法检测巨噬细胞Fc/C3b受体活性和吞噬能力,采用ELISA法检测巨噬细胞分泌能力。【结果】复方双黄连、双黄连、穿心莲的安全浓度分别为0.625~2.5、3.125~12.5和0.625~2.5 mg/mL。不同浓度复方双黄连、双黄连、穿心莲作用于巨噬细胞,细胞吞噬能力均显著高于空白对照组(P<0.05)。复方双黄连可降低LPS巨噬细胞RAW264.7活跃的吞噬能力,增强HCSS巨噬细胞RAW264.7吞噬能力;不同浓度复方双黄连可使活化的巨噬细胞Fc/C3b受体活性下降,低下的Fc/C3b受体活性增强;不同浓度复方双黄连具有促进巨噬细胞分泌一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)及溶菌酶(LZM)的作用,抑制LPS巨噬细胞NO、TNF-α、IFN-γ及白细胞介素-6(IL-6)的分泌。【结论】复方双黄连可通过活化巨噬细胞Fc/C3b受体活性以增强机体免疫力和抗炎能力,同时对已活化的巨噬细胞分泌功能起到抑制作用,以减少由于免疫功能亢进造成的机体损伤。研究结果为中兽药复方双黄连的深度开发提供了参考依据。

抗体药物Fc段结构域改造研究进展

抗体是生物体内应对外来物质入侵时产生的主要保护物质,是保护生物体的重要分子。对抗体的Fc段进行改造可有效延长抗体的半衰期,大大减少给药剂量;Fc段介导的抗体效应子的功能也可以根据疾病特殊性进行上调或下调,实现治疗效果最大化。同时,仅有Fc片段的抗体,也可具备结合抗原的能力,通过缩小抗体分子的大小,可大大减少抗体分子量大的限制,为抗体药物的开发提供了新的方向。此外,除抗肿瘤作用外,抗体分子在抗感染领域也发挥了重要的作用。对抗感染中和抗体Fc区进行工程改造,可有效促进保护性的细胞免疫效应,增强抗病毒活性并延长半衰期。本文总结了抗体Fc结构域的功能特性及优化策略,并介绍Fc结构域优化改造所介导的生物学效应及在临床治疗中的应用与发展。

新生儿Fc受体及其抑制剂在原发免疫性血小板减少症中的研究进展

原发免疫性血小板减少症(ITP)是一种由血小板自身抗体介导的自身免疫性疾病,大多数ITP患者具有免疫球蛋白G(IgG)亚型的抗血小板抗体,其通过与血小板和巨核细胞上糖蛋白的相互作用导致血小板破坏增加和抑制血小板的生成。新生儿Fc受体(FcRn)用于维持人体内IgG水平稳态。通过FcRn抑制剂靶向FcRn可以降低血液中的致病性IgG,证明了其对治疗ITP和其他自身免疫性疾病有效,并在一定程度上可改善ITP患者的血小板计数。efgartigimod、Rozanolixizumab均可与FcRn结合进而导致循环IgG减少。FcRn抑制剂还能间接延长罗米司亭的半衰期以提高治疗效果。本综述简要总结了ITP中血小板的破坏和生成抑制机制、FcRn和FcRn抑制剂作用的分子机制、FcRn抑制剂在ITP中的应用以及FcRn与罗米司亭、静脉注射Ig的联系。

免疫球蛋白G Fc段结合蛋白低表达与结肠腺癌预后指标的相关性研究

目的探究免疫球蛋白G Fc段结合蛋白(FCGBP)低表达与结肠腺癌预后指标的相关性。方法收集TCGA数据库和基因型组织表达数据库中记录结肠腺癌肿瘤组织数据的455例患者(观察组)和记录癌旁组织数据的41例患者(对照组)。以FCGBP表达水平中间值(6.126 TPM)为标准,将观察组患者分为FCGBP高表达组(228例)和FCGBP低表达组(227例)。剔除失访患者后,按照“是否发生转移”将观察组患者分为转移组(52例)和非转移组(333例)。比较FCGBP高表达组和FCGBP低表达组患者的一般资料,比较观察组和对照组FCGBP mRNA的表达水平,比较转移组、非转移组与对照组FCGBP mRNA的表达水平。采用Log-rank检验绘制Kaplan-Meier曲线对转移组和非转移组患者进行生存分析,采用Cox回归方法分析评估FCGBP基因的表达在结肠腺癌中的预后价值。对FCGBP基因的表达情况和免疫细胞浸润水平进行Spearman相关性分析并绘图。结果FCGBP高表达组中浸润深度为T3~T4、临床分期为Ⅲ~Ⅳ期、淋巴结转移和远处转移的比例均低于FCGBP低表达组,FCGBP高表达组中浸润深度为T1~T2和临床分期为Ⅰ~Ⅱ期的比例均高于FCGBP低表达组,差异均有统计学意义(均P<0.05)。转移组和非转移组FCGBP相对表达量均低于对照组[(5.0±2.1)TPM、(5.9±2.1)TPM比(9.5±1.0)TPM],转移组低于非转移组,差异均有统计学意义(均P<0.05)。Kaplan-Meier生存分析结果表明转移组的中位生存期低于非转移组(2.1年比8.2年),差异具有统计学意义(Log-rank P<0.001)。Cox回归方法分析表明FCGBP表达水平越低,患者的总生存期越短,预后越差(风险比=4.434,95%置信区间:2.778~7.077,P<0.001)。Spearman相关性分析结果表明结肠腺癌患者的FCGBP基因的表达与B淋巴细胞、CD_(4)^(+)T细胞、CD_(8)^(+)T细胞、中性粒细胞和树突状细胞的浸润水平呈正相关(均P<0.05)。结论低水平FCGBP mRNA与结肠腺癌患者不良预后有关,并且与结肠腺癌患者肿瘤免疫浸润情况关系密切,有望成为结肠腺癌预后的生物标志物。

Combining stochastic density functional theory with deep potential molecular dynamics to study warm dense matter

In traditional finite-temperature Kohn–Sham density functional theory(KSDFT),the partial occupation of a large number of high-energy KS eigenstates restricts the use of first-principles molecular dynamics methods at extremely high temperatures.However,stochastic density functional theory(SDFT)can overcome this limitation.Recently,SDFT and the related mixed stochastic–deterministic density functional theory,based on a plane-wave basis set,have been implemented in the first-principles electronic structure software ABACUS[Q.Liu and M.Chen,Phys.Rev.B 106,125132(2022)].In this study,we combine SDFT with the Born–Oppenheimer molecular dynamics method to investigate systems with temperatures ranging from a few tens of eV to 1000 eV.Importantly,we train machine-learning-based interatomic models using the SDFT data and employ these deep potential models to simulate large-scale systems with long trajectories.Subsequently,we compute and analyze the structural properties,dynamic properties,and transport coefficients of warm dense matter.

Effects of elafibranor on liver fibrosis and gut barrier function in a mouse model of alcohol-associated liver disease

BACKGROUND Alcohol-associated liver disease(ALD)is a leading cause of liver-related morbidity and mortality,but there are no therapeutic targets and modalities to prevent ALD-related liver fibrosis.Peroxisome proliferator activated receptor(PPAR)α and δ play a key role in lipid metabolism and intestinal barrier homeostasis,which are major contributors to the pathological progression of ALD.Meanwhile,elafibranor(EFN),which is a dual PPARαand PPARδagonist,has reached a phase III clinical trial for the treatment of metabolic dysfunctionassociated steatotic liver disease and primary biliary cholangitis.However,the benefits of EFN for ALD treatment is unknown.AIM To evaluate the inhibitory effects of EFN on liver fibrosis and gut-intestinal barrier dysfunction in an ALD mouse model.METHODS ALD-related liver fibrosis was induced in female C57BL/6J mice by feeding a 2.5% ethanol(EtOH)-containing Lieber-DeCarli liquid diet and intraperitoneally injecting carbon tetrachloride thrice weekly(1 mL/kg)for 8 weeks.EFN(3 and 10 mg/kg/day)was orally administered during the experimental period.Histological and molecular analyses were performed to assess the effect of EFN on steatohepatitis,fibrosis,and intestinal barrier integrity.The EFN effects on HepG2 lipotoxicity and Caco-2 barrier function were evaluated by cell-based assays.RESULTS The hepatic steatosis,apoptosis,and fibrosis in the ALD mice model were significantly attenuated by EFN treatment.EFN promoted lipolysis and β-oxidation and enhanced autophagic and antioxidant capacities in EtOH-stimulated HepG2 cells,primarily through PPARαactivation.Moreover,EFN inhibited the Kupffer cell-mediated inflammatory response,with blunted hepatic exposure to lipopolysaccharide(LPS)and toll like receptor 4(TLR4)/nuclear factor kappa B(NF-κB)signaling.EFN improved intestinal hyperpermeability by restoring tight junction proteins and autophagy and by inhibiting apoptosis and proinflammatory responses.The protective effect on intestinal barrier function in the EtOH-stimulated Caco-2 cells was predominantly mediated by PPARδ activation.CONCLUSION EFN reduced ALD-related fibrosis by inhibiting lipid accumulation and apoptosis,enhancing hepatocyte autophagic and antioxidant capacities,and suppressing LPS/TLR4/NF-κB-mediated inflammatory responses by restoring intestinal barrier function.

Tailoring MXene Thickness and Functionalization for Enhanced Room‑Temperature Trace NO_(2) Sensing

In this study,precise control over the thickness and termination of Ti3C2TX MXene flakes is achieved to enhance their electrical properties,environmental stability,and gas-sensing performance.Utilizing a hybrid method involving high-pressure processing,stirring,and immiscible solutions,sub-100 nm MXene flake thickness is achieved within the MXene film on the Si-wafer.Functionalization control is achieved by defunctionalizing MXene at 650℃ under vacuum and H2 gas in a CVD furnace,followed by refunctionalization with iodine and bromine vaporization from a bubbler attached to the CVD.Notably,the introduction of iodine,which has a larger atomic size,lower electronegativity,reduce shielding effect,and lower hydrophilicity(contact angle:99°),profoundly affecting MXene.It improves the surface area(36.2 cm^(2) g^(-1)),oxidation stability in aqueous/ambient environments(21 days/80 days),and film conductivity(749 S m^(-1)).Additionally,it significantly enhances the gas-sensing performance,including the sensitivity(0.1119Ωppm^(-1)),response(0.2% and 23%to 50 ppb and 200 ppm NO_(2)),and response/recovery times(90/100 s).The reduced shielding effect of the–I-terminals and the metallic characteristics of MXene enhance the selectivity of I-MXene toward NO2.This approach paves the way for the development of stable and high-performance gas-sensing two-dimensional materials with promising prospects for future studies.

基于FCS MPC的TNPC UPQC补偿策略研究

针对传统统一电能质量控制器(UPQC)控制策略存在控制性能不佳的问题,以三电平变流器为对象提出基于有限集模型预测控制(FCS MPC)的TNPC UPQC的补偿策略。设计了补偿量检测环节,研究了串并联侧补偿量控制策略;并通过进一步优化分区,在减少控制器计算量的同时,兼顾了三电平变流器中点电位平衡的控制效果。最后在MATLAB/Simulink仿真实验平台搭建模型,仿真结果证明了所提出的设计方法的正确性和可行性。

Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury

Spinal cord injury(SCI)often results in irreversible loss of sensory and motor functions,and most SCIs are incurable with current medical practice.One of the hardest challenges in treating SCI is the development of a dysfunctional pathological microenvironment,which mainly comprises excessive inflammation,deposition of inhibitory molecules,neurotrophic factor deprivation,glial scar formation,and imbalance of vascular function.To overcome this challenge,implantation of functional biomaterials at the injury site has been regarded as a potential treatment for modulating the dysfunctional microenvironment to support axon regeneration,remyelination at injury site,and functional recovery after SCI.This review summarizes characteristics of dysfunctional pathological microenvironment and recent advances in biomaterials as well as the technologies used to modulate inflammatory microenvironment,regulate inhibitory microenvironment,and reshape revascularization microenvironment.Moreover,technological limitations,challenges,and future prospects of functional biomaterials to promote efficient repair of SCI are also discussed.This review will aid further understanding and development of functional biomaterials to regulate pathological SCI microenvironment.

Interaction between systemic iron parameters and left ventricular structure and function in the preserved ejection fraction population:a two-sample bidirectional Mendelian randomization study

BACKGROUND Left ventricular(LV)remodeling and diastolic function in people with heart failure(HF)are correlated with iron status;however,the causality is uncertain.This Mendelian randomization(MR)study investigated the bidirectional causal relationship between systemic iron parameters and LV structure and function in a preserved ejection fraction population.METHODS Transferrin saturation(TSAT),total iron binding capacity(TIBC),and serum iron and ferritin levels were extracted as instrumental variables for iron parameters from meta-analyses of public genome-wide association studies.Individuals without myocardial infarction history,HF,or LV ejection fraction(LVEF)<50%(n=16,923)in the UK Biobank Cardiovascular Magnetic Resonance Imaging Study constituted the outcome dataset.The dataset included LV end-diastolic volume,LV endsystolic volume,LV mass(LVM),and LVM-to-end-diastolic volume ratio(LVMVR).We used a two-sample bidirectional MR study with inverse variance weighting(IVW)as the primary analysis method and estimation methods using different algorithms to improve the robustness of the results.RESULTS In the IVW analysis,one standard deviation(SD)increased in TSAT significantly correlated with decreased LVMVR(β=-0.1365;95%confidence interval[CI]:-0.2092 to-0.0638;P=0.0002)after Bonferroni adjustment.Conversely,no significant relationships were observed between other iron and LV parameters.After Bonferroni correction,reverse MR analysis showed that one SD increase in LVEF significantly correlated with decreased TSAT(β=-0.0699;95%CI:-0.1087 to-0.0311;P=0.0004).No heterogeneity or pleiotropic effects evidence was observed in the analysis.CONCLUSIONS We demonstrated a causal relationship between TSAT and LV remodeling and function in a preserved ejection fraction population.

The Probability Density Function Related to Shallow Cumulus Entrainment Rate and Its Influencing Factors in a Large-Eddy Simulation

The process of entrainment-mixing between cumulus clouds and the ambient air is important for the development of cumulus clouds.Accurately obtaining the entrainment rate(λ)is particularly important for its parameterization within the overall cumulus parameterization scheme.In this study,an improved bulk-plume method is proposed by solving the equations of two conserved variables simultaneously to calculateλof cumulus clouds in a large-eddy simulation.The results demonstrate that the improved bulk-plume method is more reliable than the traditional bulk-plume method,becauseλ,as calculated from the improved method,falls within the range ofλvalues obtained from the traditional method using different conserved variables.The probability density functions ofλfor all data,different times,and different heights can be well-fitted by a log-normal distribution,which supports the assumed stochastic entrainment process in previous studies.Further analysis demonstrate that the relationship betweenλand the vertical velocity is better than other thermodynamic/dynamical properties;thus,the vertical velocity is recommended as the primary influencing factor for the parameterization ofλin the future.The results of this study enhance the theoretical understanding ofλand its influencing factors and shed new light on the development ofλparameterization.

Combined Promoting Effects of Specific Organic Functional Groups and Alumina Surface Characteristics for the Design of a Highly Efficient NiMo/Al_(2)O_(3) Hydrodesulfurization Catalyst

To prepare a highly efficient NiMo/Al_(2)O_(3) hydrodesulfurization catalyst,the combined effects of specific organic functional groups and alumina surface characteristics were investigated.First,the correlation between the surface characteristics of four different alumina and the existing Mo species states was established.It was found that the Mo equilibrium adsorption capacity can be used as a specific descriptor to quantitatively evaluate the changes in surface characteristics of different alumina.A lower Mo equilibrium adsorption capacity for alumina means weaker metal-support interaction and the loaded Mo species are easier to transform into MoS2.However,the Mo-O-Al bonds still exist at the metal-support interface.The introduction of cationic surfactant hecadecyl trimethyl ammonium bromide(CTAB)can further improve Mo species dispersion through electrostatic attraction with Mo anions and interaction of its alkyl chain with the alumina surface;meanwhile,the introduction of ethylenediamine tetraacetic acid(EDTA)can complex with Ni ions to enhance the Ni-promoting effect on Mo.Therefore,the NiMo catalyst designed using alumina with lower Mo equilibrium adsorption capacity and the simultaneous addition of EDTA and CTAB exhibits the highest hydrodesulfurization activity for 4,6-dimethyl dibenzothiophene because of its proper metal-support interaction and more well-dispersed Ni-Mo-S active phases.

Study on the efficacy of early treatment with pirfenidone on the lung function of patients with idiopathic pulmonary fibrosis

BACKGROUND Idiopathic pulmonary fibrosis(IPF)is classified under fibrotic interstitial pneumonia,characterized by a chronic and progressive course.The predominant clinical features of IPF include dyspnea and pulmonary dysfunction.AIM To assess the effects of pirfenidone in the early treatment of IPF on lung function in patients.METHODS A retrospective analysis was performed on 113 patients with IPF who were treated in our hospital from November 2017 to January 2023.These patients were divided into two groups:control group(n=53)and observation group(n=60).In the control group,patients received routine therapy in combination with methylprednisolone tablets,while those in the observation group received routine therapy together with pirfenidone.After applying these distinct treatment approaches to the two groups,we assessed several parameters,including the overall effectiveness of clinical therapy,the occurrence of adverse reactions(e.g.,nausea,vomiting,and anorexia),symptom severity scores,pulmonary function index levels,inflammatory marker levels,and the 6-min walk distance before and after treatment in both groups.RESULTS The observation group exhibited significantly higher rates than the control group after therapy,with a clear distinction(P<0.05).After treatment,the observation group experienced significantly fewer adverse reactions than the control group,with a noticeable difference(P<0.05).When analyzing the symptom severity scores between the two groups of patients after treatment,the observation group had significantly lower scores than the control group,with a distinct difference(P<0.05).When comparing the pulmonary function index levels between the two groups of patients after therapy,the observation group displayed significantly higher levels than the control group,with a noticeable difference(P<0.05).Evaluating the inflammatory marker data(C-reactive protein,interleukin-2[IL-2],and IL-8)between the two groups of patients after therapy,the observation group exhibited significantly lower levels than the control group,with significant disparities(P<0.05).Comparison of the 6-min walking distance data between the two groups of patients after treatment showed that the observation group achieved significantly greater distances than the control group,with a marked difference(P<0.05).CONCLUSION Prompt initiation of pirfenidone treatment in individuals diagnosed with IPF can enhance pulmonary function,elevate inflammatory factor levels,and increase the distance covered in the 6-min walk test.This intervention is conducive to effectively decreasing the occurrence of adverse reactions in patients.

TIM-1-Fc融合蛋白对哮喘小鼠Th1/Th2和Th17/Treg免疫失衡的调节

目的制备T细胞免疫球蛋白和黏蛋白结构域1(T cell immunoglobulin domain and mucin domain protein-1,TIM-1)-Fc融合蛋白并探讨TIM-1-Fc融合蛋白对卵白蛋白(ovabumin,OVA)诱导的哮喘小鼠的干预作用及潜在作用机制。方法通过基因工程技术获得TIM-1-Fc融合蛋白。采用腹腔注射OVA氢氧化铝溶液致敏建立过敏性哮喘小鼠模型,随机分为对照组、哮喘组和TIM-1-Fc干预组。每次干预前20 min,使用40μL卵白蛋白生理盐水溶液(OVA-NS)滴鼻,TIM-1-Fc融合蛋白干预包括TIM-1-Fc滴鼻组(每只小鼠每次给予1μg/40μL TIM-1-Fc滴鼻)和TIM-1-Fc注射组(每只小鼠每次给予6μg/200μL TIM-1-Fc腹腔注射),每天1次,连续7 d,对照组用生理盐水替代。采用苏木精-伊红(HE)染色观察肺组织病理变化;采用流式细胞术检测小鼠外周血中辅助性T细胞2(type 2 T helper cells,Th2)、辅助性T细胞17(type 17 T helper cells,Th17)和调节性T细胞(Treg)比例及相关细胞因子水平。结果成功构建TIM-1-Fc融合蛋白,成功构建OVA诱导的过敏性哮喘小鼠模型。与哮喘组相比,TIM-1-Fc融合蛋白干预后显著减轻了哮喘小鼠气道炎性损伤和肺组织损伤;TIM-1-Fc融合蛋白干预能显著降低外周血中TIM-1^(+)CD4^(+)T细胞和TIM-1^(+)Th17细胞比例,使TIM-1^(+)Treg细胞增多,显著降低Th2、Th17细胞比例,提高Treg细胞比例,调节哮喘中Th1/Th2和Th17/Treg免疫失衡。结论TIM-1-Fc融合蛋白改善OVA诱导的过敏性哮喘小鼠气道炎症和肺组织损伤,其作用机制可能与TIM-1-Fc融合蛋白对Th1/Th2和Th17/Treg的免疫调节有关。

Amino-functionalized UiO-66-doped mixed matrix membranes with high permeation performance and fouling resistance

For the reduction of bovine serum proteins from wastewater,a novel mixed matrix membrane was prepared by functionalizing the substrate material polyaryletherketone(PAEK),followed by carboxyl groups(C-SPAEKS),and then adding amino-functionalized UiO-66-NH_(2)(Am-UiO-66-NH_(2)).Aminofunctionalization of UiO-66 was accomplished by melamine,followed by an amidation reaction to immobilize Am-UiO-66-NH_(2),which was immobilized on the surface of the membrane as well as in the pore channels,which enhanced the hydrophilicity of the membrane surface while increasing the negative potential of the membrane surface.This nanoparticle-loaded ultrafiltration membrane has good permeation performance,with a pure water flux of up to 482.3 L·m^(-2)·h^(-1) for C-SPAEKS/AmUiO-66-NH_(2) and a retention rate of up to 98.7%for bovine serum albumin(BSA)-contaminated solutions.Meanwhile,after several hydrophilic modifications,the flux recovery of BSA contaminants by this series of membranes increased from 56.2%to 80.55%of pure membranes.The results of ultra-filtration flux time tests performed at room temperature showed that the series of ultrafiltration membranes remained relatively stable over a test time of 300 min.Thus,the newly developed mixed matrix membrane showed potential for high efficiency and stability in wastewater treatment containing bovine serum proteins.

Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment

Recent studies have mostly focused on engraftment of cells at the lesioned spinal cord,with the expectation that differentiated neurons facilitate recovery.Only a few studies have attempted to use transplanted cells and/or biomaterials as major modulators of the spinal cord injury microenvironment.Here,we aimed to investigate the role of microenvironment modulation by cell graft on functional recovery after spinal cord injury.Induced neural stem cells reprogrammed from human peripheral blood mononuclear cells,and/or thrombin plus fibrinogen,were transplanted into the lesion site of an immunosuppressed rat spinal cord injury model.Basso,Beattie and Bresnahan score,electrophysiological function,and immunofluorescence/histological analyses showed that transplantation facilitates motor and electrophysiological function,reduces lesion volume,and promotes axonal neurofilament expression at the lesion core.Examination of the graft and niche components revealed that although the graft only survived for a relatively short period(up to 15 days),it still had a crucial impact on the microenvironment.Altogether,induced neural stem cells and human fibrin reduced the number of infiltrated immune cells,biased microglia towards a regenerative M2 phenotype,and changed the cytokine expression profile at the lesion site.Graft-induced changes of the microenvironment during the acute and subacute stages might have disrupted the inflammatory cascade chain reactions,which may have exerted a long-term impact on the functional recovery of spinal cord injury rats.

Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function.intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to21-d-old yellow-feathered broilers.A total of 7201-d-old yellow-feathered maleb roilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg^(-1))supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg^(-1)in the form of FeSO_(4)·7H_(2)O.Compared with the birds in the control group,birds supplemented with 20mg Fe kg^(-1)had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg^(-1)significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20mg Fe kg^(-1).Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P?0.05)as dietary Feincreased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P?0.05)or quadratically(P?0.05).Compared with the supplementation of 20 mg Fe kg^(-1),the supplementation of640 mg Fe kg^(-1)or higher increased(P?0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg^(-1)reduced(P?0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg^(-1)or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.