Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f.sp.fragaria (Fof),which infects the vascular bundles,blocking water and nutrient transport from roots to the aboveground...Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f.sp.fragaria (Fof),which infects the vascular bundles,blocking water and nutrient transport from roots to the aboveground.It is a severe pathogen which spreads rapidly and destroys strawberry production.Finding a way to control this disease is of great scientific value and practical importance.In this study,three fungi were isolated from the vascular tissues of sick strawberries in the field.After DNA sequencing,they were identified as Fof,Aspergillus fumigatus and Trichoderma harzianum,respectively,among which the first two are pathogens and the third is a probiotic.All fungi were controlled by thiophanate-methyl (TM),a commercial fungicide.On PDA medium,20 mg·L^(-1)5-aminolevulinic acid (ALA),a natural non-protein amino acid,promoted T.harzianum proliferation,but inhibited Fof and A.fumigatus.In confrontation test,the growth of Fof or A.fumigatus was inhibited by T.harzianum and exogenous ALA promoted T.harzianum growth but significantly inhibited the pathogen growth.When three species of fungi were separately or combinedly inoculated on healthy strawberry plants,T.harzianum promoted plant growth and development while Fof or A.fumigatus caused growth retardation,where Fof directly caused leaf yellowing and plant wilting.When the plants inoculated with different fungus were treated with ALA,the results turned out that ALA alleviated SFW symptoms by bidirectionally promoting T.harzianum proliferation and inhibiting Fof and A.fumigatus.Thus,ALA might be used in comprehensively controlling SFW in strawberry industry.展开更多
从香蕉园土壤中分离和筛选得到33株对香蕉枯萎病菌（Fusari um ax Cysporumf．sp．cubense）有抑制作用的拮抗菌，对其进行了抑制病原菌菌丝生长和孢子萌发的试验，结果表明：拮抗菌株G1、G3、F1、F3、11发酵液对香蕉枯萎病菌的生长有...从香蕉园土壤中分离和筛选得到33株对香蕉枯萎病菌（Fusari um ax Cysporumf．sp．cubense）有抑制作用的拮抗菌，对其进行了抑制病原菌菌丝生长和孢子萌发的试验，结果表明：拮抗菌株G1、G3、F1、F3、11发酵液对香蕉枯萎病菌的生长有显著的抑制作用，在平板上产生的抑菌圈直径为10．33~48．33mm，抑菌效果持续稳定；对孢子的萌发抑制率为92．50％～98．22％，拮抗菌的抑制作用表现为抑制病菌分生孢子的萌发，致使孢子芽管扭曲。展开更多
基金funded by the Natural Science Foundation of China (Grant No.32172512)the Jiangsu Agricultural Science and Technology Innovation Fund[Grant No.CX(20)2023]+1 种基金the Jiangsu Special Fund for Frontier Foundation Research of Carbon Peaking and Carbon Neutralization (Grant No.BK20220005)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘Strawberry Fusarium wilt (SFW) is a systematic soil-borne disease caused by Fusarium oxysporum f.sp.fragaria (Fof),which infects the vascular bundles,blocking water and nutrient transport from roots to the aboveground.It is a severe pathogen which spreads rapidly and destroys strawberry production.Finding a way to control this disease is of great scientific value and practical importance.In this study,three fungi were isolated from the vascular tissues of sick strawberries in the field.After DNA sequencing,they were identified as Fof,Aspergillus fumigatus and Trichoderma harzianum,respectively,among which the first two are pathogens and the third is a probiotic.All fungi were controlled by thiophanate-methyl (TM),a commercial fungicide.On PDA medium,20 mg·L^(-1)5-aminolevulinic acid (ALA),a natural non-protein amino acid,promoted T.harzianum proliferation,but inhibited Fof and A.fumigatus.In confrontation test,the growth of Fof or A.fumigatus was inhibited by T.harzianum and exogenous ALA promoted T.harzianum growth but significantly inhibited the pathogen growth.When three species of fungi were separately or combinedly inoculated on healthy strawberry plants,T.harzianum promoted plant growth and development while Fof or A.fumigatus caused growth retardation,where Fof directly caused leaf yellowing and plant wilting.When the plants inoculated with different fungus were treated with ALA,the results turned out that ALA alleviated SFW symptoms by bidirectionally promoting T.harzianum proliferation and inhibiting Fof and A.fumigatus.Thus,ALA might be used in comprehensively controlling SFW in strawberry industry.
文摘从香蕉园土壤中分离和筛选得到33株对香蕉枯萎病菌（Fusari um ax Cysporumf．sp．cubense）有抑制作用的拮抗菌，对其进行了抑制病原菌菌丝生长和孢子萌发的试验，结果表明：拮抗菌株G1、G3、F1、F3、11发酵液对香蕉枯萎病菌的生长有显著的抑制作用，在平板上产生的抑菌圈直径为10．33~48．33mm，抑菌效果持续稳定；对孢子的萌发抑制率为92．50％～98．22％，拮抗菌的抑制作用表现为抑制病菌分生孢子的萌发，致使孢子芽管扭曲。
