Prema 178 onion variety is widely used in production in Burkina Faso. It is greatly appreciated but susceptible to basal rot. This study aimed to evaluate the pathogenic ability of six strains of Fusarium genus identi...Prema 178 onion variety is widely used in production in Burkina Faso. It is greatly appreciated but susceptible to basal rot. This study aimed to evaluate the pathogenic ability of six strains of Fusarium genus identified in Burkina Faso on onion. Seeds, seedlings and bulbs were used for the test. A conidial suspension of each strain was made in tubes and adjusted to 1 × 10<sup>6</sup> conidia/ml with distilled water for the different tests. Germination test in the laboratory and greenhouse showed that all treatments with the strains of Fusarium oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, F. proliferatum and F. sp. induced failure to emerge and showed a significant difference with the control. The different strains also induced stunting rates of coleoptile growth. Fusarium oxysporum f. sp. cepae, F. acutatum, F. proliferatum, F. falciforme and F. solani were very aggressive, as they recorded above 50% damping-off rates. The test on the bulbs revealed that the strains were classified into two groups. The first consists of F. oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, which caused rots with respective lengths from 2.06;1.48;1.84;1.46 and 2.12 cm, thus very aggressive according to Ghanbarzadeh scale. The second is formed by F. proliferatum which recorded 0.90 cm of rot length, thus moderately aggressive. It would be appropriate to suggest a sustainable management method for these pathogens in order to improve the yield of onion production.展开更多
In order to isolate and identify the pathogens causing Fusarium wilt in bitter gourd in Hainan Province, China, four bitter gourd plants exhibiting symptoms of Fusarium wilt were collected from Tunchang County, Hainan...In order to isolate and identify the pathogens causing Fusarium wilt in bitter gourd in Hainan Province, China, four bitter gourd plants exhibiting symptoms of Fusarium wilt were collected from Tunchang County, Hainan Province. Four pathogen strains （HNTC-01, HNTC-02, HNTC-03 and HNTC-04） were isolated from the four plants, and identified via morphological observation and molecular techniques. The results showed that HNTC--01, HNTC-03 and HNTC-04 were F. oxysporum, while HNTC-02 was F. graminearum. The tests on pathogenicity and host specificity showed that HNTC-01, HNTC-03 and HNTC-04 were F. oxysporum Schl. f. sp. momordicae. Phylogenetic analysis revealed that HNTC-01, HNTC-03, HNTC-04 were clustered together with 14 strains of F. oxysporum, and HNTC-02 was clustered together with six strains of F. graminearum. In addition, rDNA-ITS region can be used to distinguish different Fusarium species, but can not be used to distinguish different biotypes within a Fusarium species. This study could provide a scientific basis for the diagnosis and prevention of wilt in bitter gourd.展开更多
文摘Prema 178 onion variety is widely used in production in Burkina Faso. It is greatly appreciated but susceptible to basal rot. This study aimed to evaluate the pathogenic ability of six strains of Fusarium genus identified in Burkina Faso on onion. Seeds, seedlings and bulbs were used for the test. A conidial suspension of each strain was made in tubes and adjusted to 1 × 10<sup>6</sup> conidia/ml with distilled water for the different tests. Germination test in the laboratory and greenhouse showed that all treatments with the strains of Fusarium oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, F. proliferatum and F. sp. induced failure to emerge and showed a significant difference with the control. The different strains also induced stunting rates of coleoptile growth. Fusarium oxysporum f. sp. cepae, F. acutatum, F. proliferatum, F. falciforme and F. solani were very aggressive, as they recorded above 50% damping-off rates. The test on the bulbs revealed that the strains were classified into two groups. The first consists of F. oxysporum f. sp. cepae, F. solani, F. falciforme, F. acutatum, which caused rots with respective lengths from 2.06;1.48;1.84;1.46 and 2.12 cm, thus very aggressive according to Ghanbarzadeh scale. The second is formed by F. proliferatum which recorded 0.90 cm of rot length, thus moderately aggressive. It would be appropriate to suggest a sustainable management method for these pathogens in order to improve the yield of onion production.
基金Supported by Central Public-interest Scientific Institution Basal Research Fund(1630032014019)National Natural Science Foundation of China(31601758)Key Research and Development Program of Hainan Province(ZDYF2016225)
文摘In order to isolate and identify the pathogens causing Fusarium wilt in bitter gourd in Hainan Province, China, four bitter gourd plants exhibiting symptoms of Fusarium wilt were collected from Tunchang County, Hainan Province. Four pathogen strains （HNTC-01, HNTC-02, HNTC-03 and HNTC-04） were isolated from the four plants, and identified via morphological observation and molecular techniques. The results showed that HNTC--01, HNTC-03 and HNTC-04 were F. oxysporum, while HNTC-02 was F. graminearum. The tests on pathogenicity and host specificity showed that HNTC-01, HNTC-03 and HNTC-04 were F. oxysporum Schl. f. sp. momordicae. Phylogenetic analysis revealed that HNTC-01, HNTC-03, HNTC-04 were clustered together with 14 strains of F. oxysporum, and HNTC-02 was clustered together with six strains of F. graminearum. In addition, rDNA-ITS region can be used to distinguish different Fusarium species, but can not be used to distinguish different biotypes within a Fusarium species. This study could provide a scientific basis for the diagnosis and prevention of wilt in bitter gourd.
基金云南省万人计划“云岭学者”项目(YNWR-YLXZ-2018-018)国际原子能机构(International Atomic Energy Agency)项目“An IntegrativeApproach to Enhance Disease Resistance Against Fusarium Wilt(Foc TR4)in Banana–Phase II”(D23033-26673)。
