[Objectives]To study the effects of fungi Fusarium sp.to rhizosphere soil and physiological characteristics of Camellia oleifera Abel.[Methods]We investigated the effects of Fusarium sp.to rhizosphere soil nutrient el...[Objectives]To study the effects of fungi Fusarium sp.to rhizosphere soil and physiological characteristics of Camellia oleifera Abel.[Methods]We investigated the effects of Fusarium sp.to rhizosphere soil nutrient element content and metabolites of C.oleifera.C.oleifera was inoculated with the suspension of Fusarium sp.in pot experiments and ammonium-N,available phosphorus,available potassi-um,organic matter,enzymes and pH of rhizosphere soil,MDA content,activity of SOD,POD of C.oleifera leaves were analyzed.[Results]Fusarium sp.stress significantly inhibited soil enzyme activities and significantly reduced available phosphorus content,especially for phospha-tase and sucrase.Antioxidant enzyme activities in C.oleifera tissues showed that Fusarium sp.stress significantly increased MDA and SOD enzyme activities and decreased POD enzyme activity.Especially,SOD enzyme activity was elevated by 53.86%compared with the CK group.In addition,analysis of the content of major metabolites in C.oleifera leaves showed that Fusarium sp.stress significantly reduced the content of total flavonoids,quercetin,isoquercitrin and isoquercitrin in C.oleifera leaves by 7.80%,50.00%and 75.90%,respectively.[Conclusions]Our results are an important step which showed strong resistance of C.oleifera and can give a novel insight for researches on the effects in the rhizosphere soil enzyme,soil nutrient elements and metabolites of C.oleifera under the Fusarium sp.too.展开更多
Fusagerins A–F(1–6),six new alkaloids including a unique one with the rare a-(N-formyl)carboxamide moiety(1),a hydantoin(imidazolidin-2,4-dione)derivative(2),and four fungerin analogues(3–6),were isolated from the ...Fusagerins A–F(1–6),six new alkaloids including a unique one with the rare a-(N-formyl)carboxamide moiety(1),a hydantoin(imidazolidin-2,4-dione)derivative(2),and four fungerin analogues(3–6),were isolated from the crude extract of the fungus Fusarium sp.,together with the known compound fungerin(7).Compound 2 was isolated as a racemate and further separated into two enantiomers on a chiral HPLC column.The structures of 1–6 were determined mainly by NMR experiments,and the absolute configuration of 1 and 2 was assigned by electronic circular dichroism(ECD)calculations.Compound 7 showed antibacterial activity against Staphylococcus aureus and Streptococcus pneumoniae,and weak cytotoxicity against the T24 cells.展开更多
A new β-resorcylic macrolide, 5'-hydroxyzearalenol (1), was isolated from the culture broth of a marine-derived fungus Fusarium sp. 05ABR26. Three known compounds, zearalenone (2), 8'-hydroxyzearalenone (3) a...A new β-resorcylic macrolide, 5'-hydroxyzearalenol (1), was isolated from the culture broth of a marine-derived fungus Fusarium sp. 05ABR26. Three known compounds, zearalenone (2), 8'-hydroxyzearalenone (3) and zearalenol (4) were also isolated. The structure and relative stereochemistry of 1 were elucidated on the basis of spectroscopic data and single-crystal X-ray diffraction data. Compound 2 displayed potent inhibitory activity against Pyricularia oryzae with a MIC value of 6.25 μg/mL, while compound 3 was much less active; however, 1 and 4 showed no obvious activity.展开更多
基金Supported by Key Field Project of Education Department of Guizhou Province(QJHKYZ[2021]044)Forestry Research Project of Guizhou Province(QLKH[2021]11)+1 种基金Project of Guizhou Provincial Characteristic Key Laboratory(QJHKY[2021]002)National Natural Science Foundation of China(41761010).
文摘[Objectives]To study the effects of fungi Fusarium sp.to rhizosphere soil and physiological characteristics of Camellia oleifera Abel.[Methods]We investigated the effects of Fusarium sp.to rhizosphere soil nutrient element content and metabolites of C.oleifera.C.oleifera was inoculated with the suspension of Fusarium sp.in pot experiments and ammonium-N,available phosphorus,available potassi-um,organic matter,enzymes and pH of rhizosphere soil,MDA content,activity of SOD,POD of C.oleifera leaves were analyzed.[Results]Fusarium sp.stress significantly inhibited soil enzyme activities and significantly reduced available phosphorus content,especially for phospha-tase and sucrase.Antioxidant enzyme activities in C.oleifera tissues showed that Fusarium sp.stress significantly increased MDA and SOD enzyme activities and decreased POD enzyme activity.Especially,SOD enzyme activity was elevated by 53.86%compared with the CK group.In addition,analysis of the content of major metabolites in C.oleifera leaves showed that Fusarium sp.stress significantly reduced the content of total flavonoids,quercetin,isoquercitrin and isoquercitrin in C.oleifera leaves by 7.80%,50.00%and 75.90%,respectively.[Conclusions]Our results are an important step which showed strong resistance of C.oleifera and can give a novel insight for researches on the effects in the rhizosphere soil enzyme,soil nutrient elements and metabolites of C.oleifera under the Fusarium sp.too.
基金the National Natural Science Foundation of China(81273395)the National Program of Drug Research and Development(2012ZX09301-003).
文摘Fusagerins A–F(1–6),six new alkaloids including a unique one with the rare a-(N-formyl)carboxamide moiety(1),a hydantoin(imidazolidin-2,4-dione)derivative(2),and four fungerin analogues(3–6),were isolated from the crude extract of the fungus Fusarium sp.,together with the known compound fungerin(7).Compound 2 was isolated as a racemate and further separated into two enantiomers on a chiral HPLC column.The structures of 1–6 were determined mainly by NMR experiments,and the absolute configuration of 1 and 2 was assigned by electronic circular dichroism(ECD)calculations.Compound 7 showed antibacterial activity against Staphylococcus aureus and Streptococcus pneumoniae,and weak cytotoxicity against the T24 cells.
基金the National Natural Science Foundation(No.30371680)of the People's Republic of China.
文摘A new β-resorcylic macrolide, 5'-hydroxyzearalenol (1), was isolated from the culture broth of a marine-derived fungus Fusarium sp. 05ABR26. Three known compounds, zearalenone (2), 8'-hydroxyzearalenone (3) and zearalenol (4) were also isolated. The structure and relative stereochemistry of 1 were elucidated on the basis of spectroscopic data and single-crystal X-ray diffraction data. Compound 2 displayed potent inhibitory activity against Pyricularia oryzae with a MIC value of 6.25 μg/mL, while compound 3 was much less active; however, 1 and 4 showed no obvious activity.