We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified fro...We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.展开更多
Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted ro...Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted roots,and discolored xylem.The disease broke out from July to August.Seven fungal isolates were obtained from symptomatic tissue and tested for pathogenicity.Isolate An7 was isolated at high frequency from the discolored vascular tissues,which caused the wilt disease on healthy 2-year-old seedlings after inoculation and eventually killed all the seedlings.We conducted molecular analysis for An7,including the partial sequences of the rDNA internal transcribed spacer,calmodulin,RNA polymerase II second largest subunit and the translation elongation factor 1-αand evaluation of the variously sized conidia,chlamydospores and conidiophores and cultural traits.The pathogen was identified as Fusarium nirenbergiae,a member of the Fusarium oxysporum species complex.To our knowledge,this is the first report of F.nirenbergiae causing wilt on A.negundo in China.展开更多
文摘We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.
基金The work was supported by the National Key Research and Development Program of China(Grant Number:2017YFD0600104).
文摘Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted roots,and discolored xylem.The disease broke out from July to August.Seven fungal isolates were obtained from symptomatic tissue and tested for pathogenicity.Isolate An7 was isolated at high frequency from the discolored vascular tissues,which caused the wilt disease on healthy 2-year-old seedlings after inoculation and eventually killed all the seedlings.We conducted molecular analysis for An7,including the partial sequences of the rDNA internal transcribed spacer,calmodulin,RNA polymerase II second largest subunit and the translation elongation factor 1-αand evaluation of the variously sized conidia,chlamydospores and conidiophores and cultural traits.The pathogen was identified as Fusarium nirenbergiae,a member of the Fusarium oxysporum species complex.To our knowledge,this is the first report of F.nirenbergiae causing wilt on A.negundo in China.
