We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified fro...We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.展开更多
Fusarium head blight(FHB)is a disease caused by several Fusarium species,notably,F.culmorum and F.grami-nearum.These pathogens adversely affect the technological and sanitary qualities of cereal grains,particularly du...Fusarium head blight(FHB)is a disease caused by several Fusarium species,notably,F.culmorum and F.grami-nearum.These pathogens adversely affect the technological and sanitary qualities of cereal grains,particularly durum wheat.Under favorable environmental conditions and in susceptible varieties,these Fusarium species can significantly reduce both the quantity and quality of crops.This study evaluated the pathogenicity of the two Fusarium species(FC2006 and FG2008)in the growth of durum wheat coleoptiles.The plant material included four commercially grown parental varieties(G9,G10,G11,G12)and eight breeding lines(G1,G2,G3,G4,G5,G6,G7,G8).In vitro tests revealed that both Fusarium species significantly reduced the coleoptile growth across the studied varieties and lines(p≤0.001).The control test had an average coleoptile length of 37.87 mm.In contrast,seeds inoculated with FC2006 had an average length of 0.62 mm,and those inoculated with FG2008 had only 0.064 mm.Although there was a slight difference in aggressiveness between the two spe-cies,it was not statistically significant(p>0.05).Some variability was also noted in the responses of the durum wheat varieties and lines.The G8 genotype showed remarkable behavior in both isolates,with an average length of 1.83 mm for FC2006 and 0.4 mm for FG2008.The other genotypes showed total inhibition of coleoptile growth(0 mm).Thesefindings highlight the importance of conducting further research on the defense mechanisms of durum wheat against Fusarium and assessing the local varieties’pathogenicity to better explore the interactions between these pathogens and durum wheat genotypes under in vitro conditions.展开更多
Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted ro...Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted roots,and discolored xylem.The disease broke out from July to August.Seven fungal isolates were obtained from symptomatic tissue and tested for pathogenicity.Isolate An7 was isolated at high frequency from the discolored vascular tissues,which caused the wilt disease on healthy 2-year-old seedlings after inoculation and eventually killed all the seedlings.We conducted molecular analysis for An7,including the partial sequences of the rDNA internal transcribed spacer,calmodulin,RNA polymerase II second largest subunit and the translation elongation factor 1-αand evaluation of the variously sized conidia,chlamydospores and conidiophores and cultural traits.The pathogen was identified as Fusarium nirenbergiae,a member of the Fusarium oxysporum species complex.To our knowledge,this is the first report of F.nirenbergiae causing wilt on A.negundo in China.展开更多
文摘We collected infection plants of rice bakanae desiease from 22 counties (cities) in Hubei Province in 1990-1992. Thirty five isolates of single spore were isolated from the plants. Fusarium species were identified from these isolates based on the method of Booth(1971) and other authors. Six kinds of medium wereused in the experiment. Color and growth rate ofcolonies as well as other characters were determinedfrom cultures grown on PSA. The results showedthat thirty one isolates were Fusarium moniliformevar. zhejiangensis, two isolates were Fusariumgramineum, one was Fusariurn oxysporum, and onewas Fusarium solani.
基金This Research was funded by la Direction Générale de la Recherche Scientifique et du Développement Technologique(DGRSDT),Algeria,and the Researchers Supporting Project number(RSP2024R390),King Saud University,Riyadh,Saudi Arabia.
文摘Fusarium head blight(FHB)is a disease caused by several Fusarium species,notably,F.culmorum and F.grami-nearum.These pathogens adversely affect the technological and sanitary qualities of cereal grains,particularly durum wheat.Under favorable environmental conditions and in susceptible varieties,these Fusarium species can significantly reduce both the quantity and quality of crops.This study evaluated the pathogenicity of the two Fusarium species(FC2006 and FG2008)in the growth of durum wheat coleoptiles.The plant material included four commercially grown parental varieties(G9,G10,G11,G12)and eight breeding lines(G1,G2,G3,G4,G5,G6,G7,G8).In vitro tests revealed that both Fusarium species significantly reduced the coleoptile growth across the studied varieties and lines(p≤0.001).The control test had an average coleoptile length of 37.87 mm.In contrast,seeds inoculated with FC2006 had an average length of 0.62 mm,and those inoculated with FG2008 had only 0.064 mm.Although there was a slight difference in aggressiveness between the two spe-cies,it was not statistically significant(p>0.05).Some variability was also noted in the responses of the durum wheat varieties and lines.The G8 genotype showed remarkable behavior in both isolates,with an average length of 1.83 mm for FC2006 and 0.4 mm for FG2008.The other genotypes showed total inhibition of coleoptile growth(0 mm).Thesefindings highlight the importance of conducting further research on the defense mechanisms of durum wheat against Fusarium and assessing the local varieties’pathogenicity to better explore the interactions between these pathogens and durum wheat genotypes under in vitro conditions.
基金The work was supported by the National Key Research and Development Program of China(Grant Number:2017YFD0600104).
文摘Boxelder maple(Acer negundo L.)is widely grown as ornamental trees in China.In 2017 in Pizhou,Jiangsu Province,China,the boxelder maple trees suffered from a disease which caused wilting and yellowing leaves,rotted roots,and discolored xylem.The disease broke out from July to August.Seven fungal isolates were obtained from symptomatic tissue and tested for pathogenicity.Isolate An7 was isolated at high frequency from the discolored vascular tissues,which caused the wilt disease on healthy 2-year-old seedlings after inoculation and eventually killed all the seedlings.We conducted molecular analysis for An7,including the partial sequences of the rDNA internal transcribed spacer,calmodulin,RNA polymerase II second largest subunit and the translation elongation factor 1-αand evaluation of the variously sized conidia,chlamydospores and conidiophores and cultural traits.The pathogen was identified as Fusarium nirenbergiae,a member of the Fusarium oxysporum species complex.To our knowledge,this is the first report of F.nirenbergiae causing wilt on A.negundo in China.