Chromosome G-banding in SituHybridization of RFLP Marker umc58 Linked with the Gene hm1 Dictating Helminthosporium carbonum Susceptibility1 in Maize

The technique of simultaneous G banding and in situ hybridization has been developed in plants for the first time.Using this technique.RFLP marker umc58 closely linked with the hm1 gene dictating Helminthosporium carbonum susceptibility1 was localized onto 1L3(chromosome 1,long arm,the third band from the centromere to the end of the arm),5L5 and 9L5.Theresults demonstrated that umc58 was a tripli cated sequence.It was deduced that umc58 probably was in a duplicated region that includes a part of Helminthosporium carbonum susceptibility genes(hm1 and hm2),as the hybridization sites of umc58 in chro mosomes 1 and 9 were those at which the genes localize.The techniques of simultaneous G banding and ISH in plants are discussed.

Eighteen-Element Antenna for Metal-Rimmed Smartphone Sub-6 GHz LTE42 Band Applications

Due to the limited space and large mutual coupling levels,the design of sub-6 GHz massive Multi-Input Multi-Output(m-MIMO)smartphone antenna system attracts antennas’designers and engineers worldwide.Therefore,this paper presents 18-element m-MIMO antenna system that covers the long-term evolution 42(LTE42)frequency band(3.4–3.6 GHz)for the fifth generation(5G)applications in metallic frame smartphones.The proposed array system is etched on the long sides of a metal rim of the mobile chassis symmetrically,which is electrically connected to the system ground plane with zero ground clearance.A low-profile frame of height 7 mm(λ/12.3)is symmetrically placed below&above the ground system level.The two frame parts(above/below the ground level)are utilized separately for the implantation of the antenna elements to achieve good space exploitation and perfect pattern diversity between elements.Orthogonal feeds are used to further increase the level of the isolation where each antenna element above the ground level is fed by a 50-ohm microstrip line,and each antenna element below the ground level is fed by a 50-ohm coplanar waveguide(CPW).The proposed antenna structure is a capacitive coupled-fed open-slot antenna with a small footprint area of 12×3.5 mm2(λ/7.2×λ/24.5,whereλrepresents the free space wavelength at 3.5 GHz).A small coupling L-shaped strip provides a capacitive coupling for the proposed 5G antenna structure.To establish the contribution of the proposed 18-element m-MIMO,the prototype of the proposed system was manufactured and successfully measured.Both measured and simulated results are shown to be in good agreement.This proves that the proposed antenna provides coverage for the LTE42 band(3.4–3.6 GHz)with acceptable isolation and efficiency.Moreover,the performance of the proposed m-MIMO were further verified via channel capacity calculation and the envelope correlation coefficient(ECC)measurement.Based on that,the proposed m-MIMO is shown to provide a desirable performance for all mMIMO parameters while it owns the largest MIMO order in mobile terminals in the open literature.

Cytogenetic Diagnosis and Analysis of the Clinical Profile of Individuals with Non-down Syndrome Intellectual Disability

Chromosomal alterations are the main causes of genetic diseases. One of the characteristics of certain genetic syndromes is ID （intellectual disability） presented by the individual in varying degrees. The study of the ID, its etiology, association or not with chromosomal abnormalities and a clinical diagnosis associated with the examination of karyotype are important aspects to consider when providing genetic counseling for families. Due to the lack of similar studies and of easy access to cytogenetic services for the Amazonian population, this study aimed to contribute to the advancement of this line of study in the state, analyzing the karyotype of individuals with ID, not Down Syndrome treated at the APAE-Manaus and identifying the main types of chromosomal alterations in the individuals analyzed. Analyzes were performed of the clinical diagnosis through investigation of the data from the interview and medical records as well as analysis of the karyotype of 31 patients with non-Down syndrome ID, attended in the APAE-Manaus. Of these subjects, 20 were female and 11 male. The results showed only 2 cases of chromosome abnormalities are detectable by classical cytogenetics, one of mosaic Turner syndrome and one a heterochromatic variant. It is therefore necessary to complement the study with the addition of molecular techniques for the investigation of microdeletions and/or other alterations not detectable through the banding technique, mainly for individuals whose dysmorphisms indicate a suspected syndrome.

Non-Adiabatic Phonon Dispersion of Metallic Single-Walled Carbon Nanotubes

Non-adiabatic effects can considerably modify the phonon dispersion of low-dimensional metallic systems.Here,these effects are studied for the case of metallic single-walled carbon nanotubes using a perturbative approach within a density-functional-based non-orthogonal tight-binding model.The adiabatic phonon dispersion was found to have logarithmic Kohn anomalies at the Brillouin zone center and at two mirror points inside the zone.The obtained dynamic corrections to the adiabatic phonon dispersion essentially modify and shift the Kohn anomalies as exemplified in the case of nanotube(8,5).Large corrections have the longitudinal optical phonon,which gives rise to the so-called G-band in the Raman spectra,and the carbon hexagon breathing phonon.The results obtained for the G-band for all nanotubes in the diameter range from 0.8 to 3.0 nm can be used for assignment of the high-frequency features in the Raman spectra of nanotube samples.