对GB 150.1~150.4—2011《压力容器》与ASME BPVC.Ⅷ.1—2015《Rules for Construction of Pressure Vessels Division 1》在碳钢、低合金钢冷成形受压件成形后热处理方面的规定进行了详细的对比解释与分析,以期更好地理解标准规范中的...对GB 150.1~150.4—2011《压力容器》与ASME BPVC.Ⅷ.1—2015《Rules for Construction of Pressure Vessels Division 1》在碳钢、低合金钢冷成形受压件成形后热处理方面的规定进行了详细的对比解释与分析,以期更好地理解标准规范中的要求,并对压力容器设计、制造工作提供一些帮助。展开更多
Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it ...Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS.展开更多
文摘对GB 150.1~150.4—2011《压力容器》与ASME BPVC.Ⅷ.1—2015《Rules for Construction of Pressure Vessels Division 1》在碳钢、低合金钢冷成形受压件成形后热处理方面的规定进行了详细的对比解释与分析,以期更好地理解标准规范中的要求,并对压力容器设计、制造工作提供一些帮助。
基金supported by the USDA ARS CRIS Project (3072– 21000–009–00D)。
文摘Greenbug(Schizaphis graminum, Rondani) is a serious insect pest in many wheat growing regions and has been infesting cereal crops in the USA for over a century. Continuous occurrence of new greenbug biotypes makes it essential to explore all greenbug resistant sources available to manage this pest. Gb1, a recessive greenbug resistance gene in DS28A, confers resistance to several economically important greenbug biotypes and is the only gene found to be resistant to greenbug biotype F. A set of 174 F_(2:3)lines from the cross DS28A × Custer was evaluated for resistance to greenbug biotype F in 2020 and 2022. Selective genotyping of the corresponding F_(2) population using single nucleotide polymorphism(SNP) markers generated by genotyping-by-sequencing(GBS) led to the identification of a candidate genomic region for Gb1. Thus, SSR markers previously mapped in this region were used to genotype the entire F2population,and kompetitive allele specific PCR(KASP) markers were also developed from SNPs in the target region.Gb1 was placed in the terminal region of the short arm of chromosome 1A, and its location was confirmed in a second population derived from the cross DS28A × PI 697274. The combined data analysis from the two mapping populations delimited Gb1 to a < 1 Mb interval between 13,328,200 and 14,241,426 bp on1AS.
