GBV C/HGV is a newly identified virus associated with human hepatitis In this study, the nucleotide sequences of the partial NS5 gene of GBV C/HGV derived from sera of 8 Chinese patien...GBV C/HGV is a newly identified virus associated with human hepatitis In this study, the nucleotide sequences of the partial NS5 gene of GBV C/HGV derived from sera of 8 Chinese patients were determined The nucleotide homology among the 8 isolates were 92% on average On the basis of sequence analysis, two sets of oligonucleotide primers derived from highly conserved region of GBV C/HGV NS5 gene were designed to establish both sensitive and specific nested PCR for detection of GBV C/HGV RNA 253 Chinese patients were examined for the virus RNA GBV C/HGV RNA positive rates in patients infected with HBV, HCV and patients with chronic non B,non C hepatitis were 18 4%, 19 8% and 8 9% respectively This result suggested that HBV,HCV and GBV C/HGV shared the same transmission risk factors 8 patients with GBV C/HGV and HCV coinfection were retrospectively observed for the response to interferon Coinfection with GBV/HGV did not negatively influence the responsiveness of HCV, and GBV C/HGV was sensitive to interferon to a certain degree展开更多
The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more ...The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more than 30% of the total cell proteins. Recombinant NS3 protein was purified by Ni-NTA column. Western blot showed that this recombinant protein could react with GBV-C/HGV RNA positive mixed sera. The recombinant GBV-C/HGV NS3 protein could be used to detect GBV-C/HGV infection and will supply material for study of structure and function of this protein.展开更多
GBV-C/HGV RNA transcripts were transcribed in vitro from a single GBV-C/HGV full-length cDNA clone pHGVqz, and injected directly into the liver of Macaca mulatta to test their infectivity. Serum samples from the e...GBV-C/HGV RNA transcripts were transcribed in vitro from a single GBV-C/HGV full-length cDNA clone pHGVqz, and injected directly into the liver of Macaca mulatta to test their infectivity. Serum samples from the experimental Macaca mulatta were collected weekly after injection to detect ALT, anti-GBV-C/HGV and GBV-C/HGV RNA. At week 6 post injection, the liver tissues of the infected Macaca mulatta were dissected through operation for histological examination. The results showed that ALT level of the Macaca mulatta remained normal and anti-GBV-C/HGV kept negative after infection. GBV-C/HGV RNA was detected positve from week 1 through week 14 post injection. The liver biopsy showed light viral-hepatitis like histological changes. From the preliminary results, we concluded that the in vitro transcripts of GBV-C/HGV may be infectious. Further studies are under way to confirm the conclusion.展开更多
文摘GBV C/HGV is a newly identified virus associated with human hepatitis In this study, the nucleotide sequences of the partial NS5 gene of GBV C/HGV derived from sera of 8 Chinese patients were determined The nucleotide homology among the 8 isolates were 92% on average On the basis of sequence analysis, two sets of oligonucleotide primers derived from highly conserved region of GBV C/HGV NS5 gene were designed to establish both sensitive and specific nested PCR for detection of GBV C/HGV RNA 253 Chinese patients were examined for the virus RNA GBV C/HGV RNA positive rates in patients infected with HBV, HCV and patients with chronic non B,non C hepatitis were 18 4%, 19 8% and 8 9% respectively This result suggested that HBV,HCV and GBV C/HGV shared the same transmission risk factors 8 patients with GBV C/HGV and HCV coinfection were retrospectively observed for the response to interferon Coinfection with GBV/HGV did not negatively influence the responsiveness of HCV, and GBV C/HGV was sensitive to interferon to a certain degree
文摘The recombinant GBV-C/HGV NS3 protein was expressed in Sf9 insect cells and analyzed by SDS-PAGE. A protein band about Mr 43810 was demonstrated on polyacrylamide gel electrophoresis and this protein amounted to more than 30% of the total cell proteins. Recombinant NS3 protein was purified by Ni-NTA column. Western blot showed that this recombinant protein could react with GBV-C/HGV RNA positive mixed sera. The recombinant GBV-C/HGV NS3 protein could be used to detect GBV-C/HGV infection and will supply material for study of structure and function of this protein.
文摘GBV-C/HGV RNA transcripts were transcribed in vitro from a single GBV-C/HGV full-length cDNA clone pHGVqz, and injected directly into the liver of Macaca mulatta to test their infectivity. Serum samples from the experimental Macaca mulatta were collected weekly after injection to detect ALT, anti-GBV-C/HGV and GBV-C/HGV RNA. At week 6 post injection, the liver tissues of the infected Macaca mulatta were dissected through operation for histological examination. The results showed that ALT level of the Macaca mulatta remained normal and anti-GBV-C/HGV kept negative after infection. GBV-C/HGV RNA was detected positve from week 1 through week 14 post injection. The liver biopsy showed light viral-hepatitis like histological changes. From the preliminary results, we concluded that the in vitro transcripts of GBV-C/HGV may be infectious. Further studies are under way to confirm the conclusion.
