Objective: To investigate the association between IL-1 Ra variable number of tandem repeat(rs2234663), IL-6-597 GA(rs1800797), IL-6-572 GC(rs1800796) and the risk of CrimeanCongo hemorrhagic fever(CCHF) in the Turkish...Objective: To investigate the association between IL-1 Ra variable number of tandem repeat(rs2234663), IL-6-597 GA(rs1800797), IL-6-572 GC(rs1800796) and the risk of CrimeanCongo hemorrhagic fever(CCHF) in the Turkish patients. Methods: This study included 50 patients infected with CCHF and 50 healthy controls. These variants were genotyped using polymerase chain reaction and/or restriction fragment length polymorphism method. Results: The distribution of the IL-6-572 GC genotypes and alleles varied significantly between the patients and the controls. The subjects carrying IL-6-572 GC GG genotype and G allele had increased risk of developing CCHF compared to the control group(P=0.006, P=0.014, respectively). IL-6-572 GC GC genotype was higher in the controls than the patients(P=0.006). For the triple genotype combinations, the 1/2-GC-GG genotype combination was detected more frequently in the control group than CCHF patients(P=0.016). IL-6(-572/-597) GG-GG genotype was significantly higher in the patient group(P=0.015), while the GC-GG genotype was significantly lower in the patient group(P=0.005). Additionally, the G-G haplotype was significantly higher in the patient group(P=0.042), whereas C-G was found to be significantly lower in the patients than the control group(P=0.037). Conclusions: The results of this study suggest the IL-6-572 GC variant might be genetic markers of sensitivity to CCHF in the Turkish population and may facilitate greater protection against the disease.展开更多
目的探讨颅脑损伤急性期联合检测C-反应蛋白(C-reactive protein,CRP)、白介素-1β(interleukin-1β)及白介素-6(interleukin-6)的急性变化与颅脑损伤程度和预后的关系。方法192例颅脑损伤患者依据格拉斯哥评分(Glasgow coma scale,GCS...目的探讨颅脑损伤急性期联合检测C-反应蛋白(C-reactive protein,CRP)、白介素-1β(interleukin-1β)及白介素-6(interleukin-6)的急性变化与颅脑损伤程度和预后的关系。方法192例颅脑损伤患者依据格拉斯哥评分(Glasgow coma scale,GCS)、出院后3个月格拉斯哥预后评分(Glasgow outcome scale,GOS)依次分组,采用免疫比浊法及双抗体夹心ABC-ELISA法测定伤后24h内血清hCRPI、L-1β及IL-6的浓度。结果伤后24h内hCRPI、L-1β及IL-6升高,随着病情的好转逐渐下降,三者与GCS呈负相关(r=-0.162,P<0.05),血清hCRP与IL-1β应用直线相关分析两者呈正相关(r=0.147,P<0.05)。血清hCRP与IL-6应用直线相关分析两者呈正相关(r=0.196,P<0.01),三项检测指标之间呈直线相关。GCS不同分组检测结果组间比较,差异有统计学意义(P<0.05)。颅脑损伤急性期血清中hCRP、IL-1β及IL-6浓度在GOS评分分组后比较差异有统计学意义(P<0.01)。结论血清中hCRPI、L-1β及IL-6是颅脑损伤患者急性期脑损伤敏感而特异的指标,联合检测对判断病情及预后、早期诊治继发性脑损害有重要意义。展开更多
文摘Objective: To investigate the association between IL-1 Ra variable number of tandem repeat(rs2234663), IL-6-597 GA(rs1800797), IL-6-572 GC(rs1800796) and the risk of CrimeanCongo hemorrhagic fever(CCHF) in the Turkish patients. Methods: This study included 50 patients infected with CCHF and 50 healthy controls. These variants were genotyped using polymerase chain reaction and/or restriction fragment length polymorphism method. Results: The distribution of the IL-6-572 GC genotypes and alleles varied significantly between the patients and the controls. The subjects carrying IL-6-572 GC GG genotype and G allele had increased risk of developing CCHF compared to the control group(P=0.006, P=0.014, respectively). IL-6-572 GC GC genotype was higher in the controls than the patients(P=0.006). For the triple genotype combinations, the 1/2-GC-GG genotype combination was detected more frequently in the control group than CCHF patients(P=0.016). IL-6(-572/-597) GG-GG genotype was significantly higher in the patient group(P=0.015), while the GC-GG genotype was significantly lower in the patient group(P=0.005). Additionally, the G-G haplotype was significantly higher in the patient group(P=0.042), whereas C-G was found to be significantly lower in the patients than the control group(P=0.037). Conclusions: The results of this study suggest the IL-6-572 GC variant might be genetic markers of sensitivity to CCHF in the Turkish population and may facilitate greater protection against the disease.
