GLP-1-IR细胞在人类胃肠道中分布的研究

目的 研究GLP-1免疫反应活性(GLP-1-IR)细胞在成人胃肠道中的分布情况。方法 应用高特异性单克隆抗体,采用免疫组化SP法,对25～65岁之间成人手术后肠道标本GLP-1-IR细胞进行定位研究。结果 证实成人肠道中GLP-1-IR细胞(L细胞)的分布以直肠最多,其次是结肠和回肠。结论 成人肠道中GLP-1-IR细胞分布不均,各段肠管L细胞的密度由近端向远端递增。

GLP-1-IR细胞在鼠、猪和人胎儿肠道的分布

选用高特异性单克隆抗 GL P- 1- IR(7- 36 )酰胺抗体 ,用免疫组织化学方法估价了 GL P- 1- IR细胞在鼠、猪和人胎儿肠道中的分布。结果发现 :在各段肠管都可见到 GL P- 1- IR细胞 ;GL P- 1- IR细胞散在于肠腺的柱状上皮细胞之间 ,多位于肠腺的基底部 ,呈开放型内分泌细胞形态 ,即 L 细胞 (EG细胞 ) ;不同种属间 GL P-1- IR细胞密度不同 ,但分布规律相同 ,即自小肠和大肠的近端向远端逐渐增大。结果表明 ,分泌 GL P- 1的 L 细胞 (GL P- 1- IR细胞 )主要分布在肠道远端 ,这提示 ,远端肠道中大量存在的 L 细胞可以接受在近端胃肠道未被吸收的营养物质的刺激 ,使 L 细胞释放 GL P-

GLP-1-IR细胞在大鼠和成人肠道的分布

目的：研究比较GLP-1-IR细胞(L细胞)在大鼠和成人肠道的分布情况；方法：应用免疫组织化学SP法，评估L细胞在Wistar大鼠和成人肠道中的分布。结果：肠道各段都可见L细胞，大鼠肠道中L细胞密度最大是回肠，成人肠道中密度最大是直肠。结论：不同种属间GLP-1-IR细胞密度不同，分布规律相同，即从小肠和大肠的近端向远端细胞密度逐渐增大。

类胰升血糖素肽-1免疫活性(GLP-1-IR)细胞分布的研究进展

类胰升血糖素 1(GLP 1)是迄今为止分离出来的作用最强的肠促胰岛素 ,其免疫活性 (GLP 1 IR)细胞广泛分布于肠道、胃、胰腺和脑等组织中 ,本文对GLP 1

类胰升血糖素肽-1免疫活性(GLP-1-IR)细胞的分布

Ⅱ型糖尿病(NIDDM)是危害人类健康的重要疾病之一。常见于中老年人,世界卫生组织已将NIDDM列为二十一世纪重点攻关疾病之首。类胰升血糖素肽-1(GIP-1)是新发现的一种内源性胰岛素刺激肽,它是迄今为止分离出来的作用最强的肠促胰岛素,将成为治疗Ⅱ型糖尿病最有效的药物。 生理性GLP-1是由30个氨基酸(未酰胺化的是由31个氨基酸)组成的肽链,是胰升血糖素前体在肠道L细胞加工而成,由肠道L细胞分泌,并在餐后释放入血。

GLP-1-IR细胞在人类胎儿和成人胃肠道的分布

目的 :对国人胎儿和成人类胰升血糖素 - 1免疫反应细胞 ( GL P- 1- IR细胞 )在胃肠道中的分布作详细的研究。方法 :应用高特异性单克隆抗 GL P- 1- IR( 7- 3 6)酰胺抗体 ,采用免疫组化技术。结果 :发现在胎儿和成人的肠管中均可见到 GL P- 1- IR细胞 ( L细胞 )的存在 ,但在生长发育的不同时期肠道 L细胞的密度不同 ,各段肠管的密度也不同 ,但不同时期的 L 细胞的分布规律相同 ,均自小肠和大肠的近段向远端逐渐增大 ,密度最大为直肠。结论 :人类肠道中的 GL P- 1- IR细胞分布规律不变 。

Effects of extracellular matrix proteins on expansion, proliferation and insulin-producing-cell differentiation of ARIP cells

Regeneration of transplantable pancreatic islet cells has been considered to be a promising alternative therapy for type 1 diabetes. Re-search has suggested that adult pancreatic stem and progenitor cells can be derived into insulin-producing cells or cultivated islet-like clusters given appropriate stimulating condi- tions. In this study we explored the effect of selective extracellular matrix (ECM) proteins on the potential of insulin-producing cell differen-tiation using ARIP cells, an adult rat pancreatic ductal epithelial cell line, as a model in vitro. Quantitative single cell morphology analysis indicated that all the four ECM proteins we have used (type I collagen, laminin, fibronectin and vitronectin) increased the single cell area and diameter of ARIP cells. In addition, se-rum-free cell cultivation was dependent on cell density and particular components;and serum could be replaced when systematic optimisa-tion could be performed. Surface treated with laminin was shown to be able to enhance overall cell expansion in the presence of de-fined serum-free medium conditions. Collagen treated surfaces enhanced insulin production in the presence of GLP-1 although the insulin gene expression was however weak accord-ingly. Our results suggest that selective ECM proteins have effects on single cell morphol-ogy, adhesion and proliferation of ARIP cells. These ECM molecules however do not have a potent effect on the insulin-producing cell dif-ferentiation potential of ARIP cells even com-bining with GLP-1.

Glucagon-like peptide-1 receptor agonists rescued diabetic vascular endothelial damage through suppression of aberrant STING signaling

Glucagon-like peptide-1 receptor agonists(GLP-1 RAs)protect against diabetic cardiovascular diseases and nephropathy.However,their activity in diabetic retinopathy(DR)remains unclear.Our retrospective cohort study involving 1626 T2DM patients revealed superior efficacy of GLP-1 RAs in controlling DR compared to other glucose-lowering medications,suggesting their advantage in DR treatment.By single-cell RNA-sequencing analysis and immunostaining,we observed a high expression of GLP-1R in retinal endothelial cells,which was down-regulated under diabetic conditions.Treatment of GLP-1 RAs significantly restored the receptor expression,resulting in an improvement in retinal degeneration,vascular tortuosity,avascular vessels,and vascular integrity in diabetic mice.GO and GSEA analyses further implicated enhanced mitochondrial gene translation and mitochondrial functions by GLP-1 RAs.Additionally,the treatment attenuated STING signaling activation in retinal endothelial cells,which is typically activated by leaked mitochondrial DNA.Expression of STING mRNA was positively correlated to the levels of angiogenic and inflammatory factors in the endothelial cells of human fibrovascular membranes.Further investigation revealed that the cAMP-responsive element binding protein played a role in the GLP-1R signaling pathway on suppression of STING signaling.This study demonstrates a novel role of GLP-1 RAs in the protection of diabetic retinal vasculature by inhibiting STING-elicited inflammatory signals.

胰高血糖素样肽1抗动脉粥样硬化的研究进展

自胰高血糖素样肽1（glucagon-like pe ptide-1,GLP-1）发现以来,国内外研究焦点主要集中在降糖疗效上。糖尿病主要并发症是动脉粥样硬化（athero-sclerosis,As）,GLP-1对As及其心血管风险是否有防治作用受到高度关注[1-2]。As病变触发于内膜,伴有血管平滑肌细胞（vascular smooth muscle cell,VSMC）的增生迁移和免疫细胞浸润[3]。

利拉鲁肽延缓2型糖尿病进展的潜在机制:中国学者的研究证据

胰岛β细胞功能障碍和IR是T2DM发生发展的重要因素。因此,保护β细胞功能和改善IR将可望延缓T2DM的进展。然而,传统降糖药物单药治疗对于延缓T2DM进展的作用非常有限。最新的研究显示,利拉鲁肽可通过多种机制保护β细胞功能和改善IR,从而延缓T2DM进展。本文将对我国学者在该领域中的相关研究进展进行综述。