The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize ...The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.展开更多
Genetic modification techniques,in particular novel gene editing technologies,hold the yet unfulfilled promise of altering genetic traits in farm animals more efficiently than by crossbreeding,allowing for a more rapi...Genetic modification techniques,in particular novel gene editing technologies,hold the yet unfulfilled promise of altering genetic traits in farm animals more efficiently than by crossbreeding,allowing for a more rapid development of new cattle breeds with distinct traits.Gene editing technologies allow for the directed alteration of specific traits and thereby have the potential to enhance,for instance,disease resilience,production yield and the production of desired substances in milk.The potential implications of these technological advancements,which are often combined with animal cloning methods,are discussed both for animal health and for consumer safety,also with consideration of available methods for the detection and identification of the related products in the food supply chain.Finally,an overview is provided of current regulatory approaches in the European Union(EU)and major countries exporting beef to the EU,for products from animals bred through established practices as well as modern biotechnologies.展开更多
基金Supported by the Funds of the Scientific Research Foundation of Northeast Agricultural University(2010RCB53)
文摘The use of genetically modified organisms （GMOs） as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM （genetically modified） products. Three anti-herbicide genes （CP4- EPSPS, BAR and PAT） are common exogenous genes used in commercialized transgenic soybean, maize and rice, In the present study, a new SYBR Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products （soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam） using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.
基金supported by the Ministry of AgricultureNature and Food Quality of the Netherlands(WOT-02-002001/002)。
文摘Genetic modification techniques,in particular novel gene editing technologies,hold the yet unfulfilled promise of altering genetic traits in farm animals more efficiently than by crossbreeding,allowing for a more rapid development of new cattle breeds with distinct traits.Gene editing technologies allow for the directed alteration of specific traits and thereby have the potential to enhance,for instance,disease resilience,production yield and the production of desired substances in milk.The potential implications of these technological advancements,which are often combined with animal cloning methods,are discussed both for animal health and for consumer safety,also with consideration of available methods for the detection and identification of the related products in the food supply chain.Finally,an overview is provided of current regulatory approaches in the European Union(EU)and major countries exporting beef to the EU,for products from animals bred through established practices as well as modern biotechnologies.
