Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immu...Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immune cell functions in vitro. We investigated the association of GNB3 genotypes with treatment response in HCV- infected patients. Methods: We genotyped 1781 HCV- free blood donors and 232 HCV- infected patients treated with interferon- alfa/ ribavirin. Sustained virologic response (SVR) was defined by undetectable HCVRNA 24 weeks after discontinuation of therapy. Non- response (NR) was defined by positive HCV- RNA at the end of at least 24 weeks of treatment. GNB3 genotypes were determined by DNA restriction enzyme analyses. Results: Genotype distribution was not significantly different in healthy controls and HCV- infected patients. Only in HCV genotype 1- infected patients a significant correlation between GNB3 CC genotype and NR could be observed (6 TT, 42 TC, 54 CC) versus SVR (11 TT, 25 TC, 19 CC) patients (P=0.004). In a logistic regression analysis including biochemical and virologic characteristics, only GNB3 CC genotype was significantly associated with NR (OR 4.9; 95% CI=1.4- 16.5; P=0.011). Conclusions: The GNB3 825 CC genotype is associated with NR in HCV- 1- infected patients.展开更多
文摘Background/Aims: The outcome of infection with the hepatitis C virus (HCV) has been shown to be influenced by genetic host factors. The G protein β 3 subunit (GNB3) C825T polymorphism has been shown to determine immune cell functions in vitro. We investigated the association of GNB3 genotypes with treatment response in HCV- infected patients. Methods: We genotyped 1781 HCV- free blood donors and 232 HCV- infected patients treated with interferon- alfa/ ribavirin. Sustained virologic response (SVR) was defined by undetectable HCVRNA 24 weeks after discontinuation of therapy. Non- response (NR) was defined by positive HCV- RNA at the end of at least 24 weeks of treatment. GNB3 genotypes were determined by DNA restriction enzyme analyses. Results: Genotype distribution was not significantly different in healthy controls and HCV- infected patients. Only in HCV genotype 1- infected patients a significant correlation between GNB3 CC genotype and NR could be observed (6 TT, 42 TC, 54 CC) versus SVR (11 TT, 25 TC, 19 CC) patients (P=0.004). In a logistic regression analysis including biochemical and virologic characteristics, only GNB3 CC genotype was significantly associated with NR (OR 4.9; 95% CI=1.4- 16.5; P=0.011). Conclusions: The GNB3 825 CC genotype is associated with NR in HCV- 1- infected patients.
