Gradient index materials have been developed from glass melting process to GRIN lenses. Two kinds of GRIN lenses have been fabricated by ion-exchange and glass fusion/diffusion. The main applications to GRIN lenses ar...Gradient index materials have been developed from glass melting process to GRIN lenses. Two kinds of GRIN lenses have been fabricated by ion-exchange and glass fusion/diffusion. The main applications to GRIN lenses are demonstrated.展开更多
Simple single-lens equivalent systems for graded-index （GRIN） lenses in inhomogeneous medium obtained using matrix optics are proposed in this letter. Due to its simplicity, the equivalent optical system enables qui...Simple single-lens equivalent systems for graded-index （GRIN） lenses in inhomogeneous medium obtained using matrix optics are proposed in this letter. Due to its simplicity, the equivalent optical system enables quick analysis of the imaging properties of GRIN lens rod immersed in inhomogeneous medium. This facilitates the optical analysis of complicated optoelectronics systems in inhomogeneous medium utilizing GRIN lens rods.展开更多
With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in tradition...With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index （GRIN） probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRiN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube （PMT） to detect fluorescence signals. Experimental results showed that this system using two-photon excitation could implement dynamic fluorescence microendoscopic imaging and monitor the movement of blood flow beneath the skin in anesthetized mice while producing images with higher contrast and signal to noise ratio （SNR） than those using one photon excitation. It would be a useful tool for studying biological processes of small animals or plants in vivo.展开更多
Fabrication method and device of ultra-small gradient-index （GRIN） fiber probe were investigated in order to explore the development of ultra-small probes for optical coherence tomography （OCT） imaging. The beam- ...Fabrication method and device of ultra-small gradient-index （GRIN） fiber probe were investigated in order to explore the development of ultra-small probes for optical coherence tomography （OCT） imaging. The beam- expanding effect of no-core fiber （NCF） and the focusing properties of the GRIN fiber lens were analyzed based on the model of GRIN fiber probe consisting of single-mode fiber （SMF）, NCF and GRIN fiber lens. A stereo micro- scope based system was developed to fabricate the GRIN fiber probe. A fiber fusion splicer and an ultrasonic cleaver were used to weld and cut the fiber respectively. A con- focal microscopy was used to measure the dimensions of probe components. The results show that the sizes of probe components developed are at the level of millimeter. Therefore, the proposed experimental system meets the fabrication requirements of an ultra-small self-focusing GRIN fiber probe. This shows that this fabrication device and method can be employed in the fabrication of ultra- small self-focusing GRIN fiber probe and applied in the study of miniaturized optical probes and OCT systems.展开更多
文摘Gradient index materials have been developed from glass melting process to GRIN lenses. Two kinds of GRIN lenses have been fabricated by ion-exchange and glass fusion/diffusion. The main applications to GRIN lenses are demonstrated.
文摘Simple single-lens equivalent systems for graded-index （GRIN） lenses in inhomogeneous medium obtained using matrix optics are proposed in this letter. Due to its simplicity, the equivalent optical system enables quick analysis of the imaging properties of GRIN lens rod immersed in inhomogeneous medium. This facilitates the optical analysis of complicated optoelectronics systems in inhomogeneous medium utilizing GRIN lens rods.
文摘With the rapid development of life sciences, there is an increasing demand for intravital fluorescence imaging of small animals. However, large dimensions and limited working distances of objective lenses in traditional fluorescence microscopes have limited their imaging applications mostly to superficial tissues. To overcome these disadvantages, researchers have developed the graded-index （GRIN） probes with small diameters for imaging internal organs of small animals in a minimally invasive fashion. However, dynamic imaging based on GRIN lens has not been studied extensively. Here, this paper presented a fluorescence endoscopic imaging system based on GRiN lenses using one-photon and two-photon excitation. GRIN lenses with 1.15 mm diameter and 7.65 mm length were used in the system. The images were acquired by a compact laser scanning imaging system with a resonant galvo-mirror system to scan the laser beam and a photomultiplier tube （PMT） to detect fluorescence signals. Experimental results showed that this system using two-photon excitation could implement dynamic fluorescence microendoscopic imaging and monitor the movement of blood flow beneath the skin in anesthetized mice while producing images with higher contrast and signal to noise ratio （SNR） than those using one photon excitation. It would be a useful tool for studying biological processes of small animals or plants in vivo.
基金supported by the National Natural Science Foundation of China(Grant No.41104065)the Dawn Planning Foundation of Shanghai Municipal Education Commission (Grant No.12CG047)by the Scientific Research Innovation Project of Shanghai Municipal Education Commission(Grant No. 13YZ022)
文摘Fabrication method and device of ultra-small gradient-index （GRIN） fiber probe were investigated in order to explore the development of ultra-small probes for optical coherence tomography （OCT） imaging. The beam- expanding effect of no-core fiber （NCF） and the focusing properties of the GRIN fiber lens were analyzed based on the model of GRIN fiber probe consisting of single-mode fiber （SMF）, NCF and GRIN fiber lens. A stereo micro- scope based system was developed to fabricate the GRIN fiber probe. A fiber fusion splicer and an ultrasonic cleaver were used to weld and cut the fiber respectively. A con- focal microscopy was used to measure the dimensions of probe components. The results show that the sizes of probe components developed are at the level of millimeter. Therefore, the proposed experimental system meets the fabrication requirements of an ultra-small self-focusing GRIN fiber probe. This shows that this fabrication device and method can be employed in the fabrication of ultra- small self-focusing GRIN fiber probe and applied in the study of miniaturized optical probes and OCT systems.
