Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP mo...Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP model, G\|GTP is used for the real\|3D modeling of subsurface geological bodies, and E\|GTP is used for the real\|3D modeling of subsurface engineering excavations.In the light of the discussions on the features and functions of E 2 GIS, the modeling principles of G\|GTP and E\|GTP are introduced. The two models couple together seamlessly to form an integral model for subsurface spatial objects including both geological bodies and excavations. An object\|oriented integral real\|3D data model and integral spatial topological relations are discussed.展开更多
目的探讨G蛋白信号调节因子5(regulator of G protein signaling 5,RGS5)表达变化与大鼠胶质瘤生长之间的关系。方法 72只Sprague-Dawley(SD)大鼠随机平均分为胶质瘤组、生理盐水组、健康对照组、腺病毒-大鼠增殖抑制基因-绿色荧光蛋白...目的探讨G蛋白信号调节因子5(regulator of G protein signaling 5,RGS5)表达变化与大鼠胶质瘤生长之间的关系。方法 72只Sprague-Dawley(SD)大鼠随机平均分为胶质瘤组、生理盐水组、健康对照组、腺病毒-大鼠增殖抑制基因-绿色荧光蛋白重组复合物(adenovirus-rat hyperplasia suppressor gene-green fluorescent protein,Adv-r HSG-GFP)组。胶质瘤组和Adv-r HSG-GFP组建立大鼠C6胶质瘤动物模型,生理盐水组颅内注射10μL生理盐水,健康对照组为健康大鼠。Adv-r HSG-GFP组在接种C6胶质瘤细胞后第4和11天,于肿瘤原位注射重组腺病毒Adv-r HSG-GFP。接种C6胶质瘤细胞后第9、15和21天取脑胶质瘤观察肿瘤的生长情况,免疫组织化学法检测RGS5蛋白的表达。结果 Adv-r HSG-GFP组肿瘤体积小于胶质瘤组(P<0.01)。生理盐水组和健康对照组无肿瘤生长。接种后第15和21天胶质瘤组RGS5蛋白表达均高于生理盐水组、健康对照组和Adv-r HSG-GFP组(均P<0.01)。结论 RGS5高表达与大鼠胶质瘤生长密切相关。展开更多
目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组...目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组(癫发作后1、3、7、14、30、60 d),荧光定量聚合酶链反应、Western blotting法和免疫组织化学染色检测各观察时间点大鼠海马组织GIT1表达变化。结果癫组大鼠GIT1 m RNA自癫持续状态后急性期第1和3天即升高(P=0.012,0.002),至潜伏期第7和14天持续升高(P=0.003,0.001),至慢性期第30和60天达峰值水平(均P=0.000);GIT1蛋白自急性期即升高,慢性期持续升高,但与对照组相比,差异未达统计学意义(均P>0.05),至慢性期达峰值水平(均P=0.000)。至慢性期第30天时,癫组大鼠海马CA1区、齿状回和海马旁皮质GIT1蛋白表达水平均高于对照组(P=0.000)。结论癫大鼠海马组织GIT1表达上调,可能通过调节细胞骨架动态重组而影响兴奋性神经网络,从而参与癫的发生。展开更多
文摘Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP model, G\|GTP is used for the real\|3D modeling of subsurface geological bodies, and E\|GTP is used for the real\|3D modeling of subsurface engineering excavations.In the light of the discussions on the features and functions of E 2 GIS, the modeling principles of G\|GTP and E\|GTP are introduced. The two models couple together seamlessly to form an integral model for subsurface spatial objects including both geological bodies and excavations. An object\|oriented integral real\|3D data model and integral spatial topological relations are discussed.
文摘目的观察G蛋白耦联受体激酶相关蛋白1(GIT1)在氯化锂-匹罗卡品致模型大鼠海马组织中的表达变化,以探讨GIT1在癫发生发展过程中的作用机制。方法共42只无特定病原体级成年雄性Wistar大鼠,制备氯化锂-匹罗卡品致模型,随机分为对照组和癫组(癫发作后1、3、7、14、30、60 d),荧光定量聚合酶链反应、Western blotting法和免疫组织化学染色检测各观察时间点大鼠海马组织GIT1表达变化。结果癫组大鼠GIT1 m RNA自癫持续状态后急性期第1和3天即升高(P=0.012,0.002),至潜伏期第7和14天持续升高(P=0.003,0.001),至慢性期第30和60天达峰值水平(均P=0.000);GIT1蛋白自急性期即升高,慢性期持续升高,但与对照组相比,差异未达统计学意义(均P>0.05),至慢性期达峰值水平(均P=0.000)。至慢性期第30天时,癫组大鼠海马CA1区、齿状回和海马旁皮质GIT1蛋白表达水平均高于对照组(P=0.000)。结论癫大鼠海马组织GIT1表达上调,可能通过调节细胞骨架动态重组而影响兴奋性神经网络,从而参与癫的发生。
