[Objectives] The research aimed to study the impact on extraction effect of polysaccharide from Ganoderma lucidum( lingzhi) by different pretreatment methods. [Methods] The impacts on extraction of G. lucidum polysacc...[Objectives] The research aimed to study the impact on extraction effect of polysaccharide from Ganoderma lucidum( lingzhi) by different pretreatment methods. [Methods] The impacts on extraction of G. lucidum polysaccharide by soaking,microwave and air flow fine pulverization were contrasted,and the extraction effect of G. lucidum polysaccharide by combining the optimal pretreatment manner with hot water extraction,alcohol extraction,alkali extraction,ultrasonic binding enzyme extraction,and microwave extraction was compared. Finally,the property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. [Results] The optimal pretreatment method was air flow fine pulverization. Compared with traditional method-direct extraction( coarse grinding combining hot water extraction),crude yield changed little,while polysaccharide content and yield were improved by 114% and 104%. The best combination manner was air flow fine pulverization pretreatment combining with alkali extraction. Compared with traditional method,crude yield,polysaccharide content and yield were improved by 76%,78% and 215% respectively. The property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. It was found that the treatment method had little impact on the property of G. lucidum polysaccharide. [Conclusions]Air flow fine pulverization pretreatment could greatly improve extraction effect of G. lucidum polysaccharide,which mainly improved the content and yield of G. lucidum polysaccharide,and extraction was more complete,with less impact on the property of the extracted polysaccharide. It was speculated that air flow fine pulverization pretreatment mainly destroyed mechanical support wall membrane structure of G. lucidum fine powder,making that intracellular functional substances completely dissolved out of the cell,and the content would be studied in follow-up experiment.展开更多
In the present work,an enzyme assisted extraction method is used to isolate crude polysaccharides from Ganoderma lucidum. The isolating effect was optimized with orthographic graph statistic method with three levels a...In the present work,an enzyme assisted extraction method is used to isolate crude polysaccharides from Ganoderma lucidum. The isolating effect was optimized with orthographic graph statistic method with three levels and four independent variables. Complex enzyme,extraction temperature,extraction time and extraction pH were combined to obtain the best possible combination to get maximum amount of extract and crude polysaccharides yield. The optimum extraction conditions were:complex enzyme amount of 3 %(w/v),extraction temperature at 45 ℃,extraction time of 3 h and extraction pH at 7. Under these conditions,the experimental amount of extract is 8.9 % and the yield of crude polysaccharides is 1.1 %,which are in close agreement with the value predicted by the model.展开更多
[Objectives] To study the in vitro antioxidant activity of hot water extracts from 7 different sources of Ganoderma lucidum mushroom.[Methods] The in vitro antioxidant activity of hot water extracts from 7 different s...[Objectives] To study the in vitro antioxidant activity of hot water extracts from 7 different sources of Ganoderma lucidum mushroom.[Methods] The in vitro antioxidant activity of hot water extracts from 7 different sources of G. lucidum was studied from total reducing capacity,scavenging of hydroxyl free radical( ·OH),superoxide anion( O_2^(2-)·),and DPPH free radical.[Results]Under the same concentration,hot water extracts wild G. lucidum in Maoer Mountain showed the strongest reducing capacity with the absorbency being 2. 48,the highest scavenging capacity of hydroxyl free radical( · OH) with the scavenging rate being 99. 46%,and the strongest scavenging ability of DPPH free radical with its scavenging rate being 96. 62%. The ability of scavenging superoxide anion free radical( O_2^(2-)·) ranked the third.[Conclusions]The hot water extracts from Maoer Mountain wild G. lucidum showed the highest antioxidant ability in three aspects,indicating that Maoer Mountain wild G. lucidum has high development potential.展开更多
[ Objective ] The research aimed to develop anti-aging masks with aqueous extracts of Ganoderma lucidum and to study the clinical efficacy on the delaying of skin aging. [Method] The anti-aging efficacy of the aqueous...[ Objective ] The research aimed to develop anti-aging masks with aqueous extracts of Ganoderma lucidum and to study the clinical efficacy on the delaying of skin aging. [Method] The anti-aging efficacy of the aqueous extract of C.anoderrna luciclum was evaluated by sensory evaluation, safety evaluation, instrumental measurements, etc. [ Result] The results of sensory evaluation showed that after continuous application of the test materials for 12 weeks, the skin dryness, elasticity, surface sheen, fine wrinkles and smoothness were well improved, and the improvement rates were 9l. 17%, 91.17% , 94.12% , 11.76% and 91.18% , respectively. The measurement results by specialized instrument showed that the skin hydration on the target site increased continuously with the prolonging of the using time. Significant differences existed between each time point after using the products and the time before using. There was no signiScant difference existed in elasticity index 112 and R5 compared with before using. [Result] Clinical data showed that the active ingredients in aqueous extract of Ganoderma lucidum had a certain effect on the delaying of skin aging.展开更多
Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum. In this work,polysaccharides were extracted from Ganoderma lucidum with boiling water method and enzyme assisted metho...Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum. In this work,polysaccharides were extracted from Ganoderma lucidum with boiling water method and enzyme assisted method. The human liver hepatocellular carcinoma cell line HepG2 was used to compare the antitumor effect of the two kinds of extraction with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) test. Both of these two kinds of Ganoderma lucidum polysaccharides reduced cell viability of cancer cell HepG2 in a dose and time-dependent manner. At low concentrations,there was no significant difference in the effectiveness of L1 and L2;while at concentrations over 0.8 μg/mL,the difference in the effectiveness of L2 in comparison to L1 became significant. At the concentrations of 3.2 μg/mL,the cancer cells were almost killed in 2 d.展开更多
The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing(HSCS) pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron m...The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing(HSCS) pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron microscope (SEM), Fourier transform infrared spectrophotometry (FTIR). Ultraviolet-visible pectrophotometer was used to determine the extraction ratio of aqueous solubility polysaccharide between the raw and broken spores. The immunological function on the mice before and after the breaking of spores wan investigated. The experimental results show that after being ground, the sporoderm-broken ratio reachs 100%, the original active ingredients of ganoderma lucidum spores do not change, and the extraction ratio of aqueous solubility polysaccharide is greatly increased by 40.08%. The broken spores show much higher immunological activity comparing with original spores of Ganoderma lucidum.展开更多
文摘[Objectives] The research aimed to study the impact on extraction effect of polysaccharide from Ganoderma lucidum( lingzhi) by different pretreatment methods. [Methods] The impacts on extraction of G. lucidum polysaccharide by soaking,microwave and air flow fine pulverization were contrasted,and the extraction effect of G. lucidum polysaccharide by combining the optimal pretreatment manner with hot water extraction,alcohol extraction,alkali extraction,ultrasonic binding enzyme extraction,and microwave extraction was compared. Finally,the property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. [Results] The optimal pretreatment method was air flow fine pulverization. Compared with traditional method-direct extraction( coarse grinding combining hot water extraction),crude yield changed little,while polysaccharide content and yield were improved by 114% and 104%. The best combination manner was air flow fine pulverization pretreatment combining with alkali extraction. Compared with traditional method,crude yield,polysaccharide content and yield were improved by 76%,78% and 215% respectively. The property of G. lucidum polysaccharide obtained after air flow fine pulverization pretreatment was detected and analyzed by high performance liquid chromatography. It was found that the treatment method had little impact on the property of G. lucidum polysaccharide. [Conclusions]Air flow fine pulverization pretreatment could greatly improve extraction effect of G. lucidum polysaccharide,which mainly improved the content and yield of G. lucidum polysaccharide,and extraction was more complete,with less impact on the property of the extracted polysaccharide. It was speculated that air flow fine pulverization pretreatment mainly destroyed mechanical support wall membrane structure of G. lucidum fine powder,making that intracellular functional substances completely dissolved out of the cell,and the content would be studied in follow-up experiment.
基金supported by Jiangsu Alphay Biological Technology Co.Ltd
文摘In the present work,an enzyme assisted extraction method is used to isolate crude polysaccharides from Ganoderma lucidum. The isolating effect was optimized with orthographic graph statistic method with three levels and four independent variables. Complex enzyme,extraction temperature,extraction time and extraction pH were combined to obtain the best possible combination to get maximum amount of extract and crude polysaccharides yield. The optimum extraction conditions were:complex enzyme amount of 3 %(w/v),extraction temperature at 45 ℃,extraction time of 3 h and extraction pH at 7. Under these conditions,the experimental amount of extract is 8.9 % and the yield of crude polysaccharides is 1.1 %,which are in close agreement with the value predicted by the model.
基金Supported by the Program of Investigation on Large Fungi Resources in Maoer Mountain which was provided by Guangxi Maoer Mountain National Nature Reserve Administration Bureau
文摘[Objectives] To study the in vitro antioxidant activity of hot water extracts from 7 different sources of Ganoderma lucidum mushroom.[Methods] The in vitro antioxidant activity of hot water extracts from 7 different sources of G. lucidum was studied from total reducing capacity,scavenging of hydroxyl free radical( ·OH),superoxide anion( O_2^(2-)·),and DPPH free radical.[Results]Under the same concentration,hot water extracts wild G. lucidum in Maoer Mountain showed the strongest reducing capacity with the absorbency being 2. 48,the highest scavenging capacity of hydroxyl free radical( · OH) with the scavenging rate being 99. 46%,and the strongest scavenging ability of DPPH free radical with its scavenging rate being 96. 62%. The ability of scavenging superoxide anion free radical( O_2^(2-)·) ranked the third.[Conclusions]The hot water extracts from Maoer Mountain wild G. lucidum showed the highest antioxidant ability in three aspects,indicating that Maoer Mountain wild G. lucidum has high development potential.
基金Supported by Comprehensive Strategic Cooperation Project of Chinese Academy of Sciences in Guangdong Province(2011B090300075)Guangzhou Special Project with Benefaction for People(2014Y2-00130)Fund Project of Transformation of Agricultural Science and Technology Achievements(2013GB2E000360)
文摘[ Objective ] The research aimed to develop anti-aging masks with aqueous extracts of Ganoderma lucidum and to study the clinical efficacy on the delaying of skin aging. [Method] The anti-aging efficacy of the aqueous extract of C.anoderrna luciclum was evaluated by sensory evaluation, safety evaluation, instrumental measurements, etc. [ Result] The results of sensory evaluation showed that after continuous application of the test materials for 12 weeks, the skin dryness, elasticity, surface sheen, fine wrinkles and smoothness were well improved, and the improvement rates were 9l. 17%, 91.17% , 94.12% , 11.76% and 91.18% , respectively. The measurement results by specialized instrument showed that the skin hydration on the target site increased continuously with the prolonging of the using time. Significant differences existed between each time point after using the products and the time before using. There was no signiScant difference existed in elasticity index 112 and R5 compared with before using. [Result] Clinical data showed that the active ingredients in aqueous extract of Ganoderma lucidum had a certain effect on the delaying of skin aging.
基金supported financially by Jiangsu Alphay Biological Technology Co.Ltd
文摘Polysaccharides are the most important pharmacologically active constituents of Ganoderma lucidum. In this work,polysaccharides were extracted from Ganoderma lucidum with boiling water method and enzyme assisted method. The human liver hepatocellular carcinoma cell line HepG2 was used to compare the antitumor effect of the two kinds of extraction with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) test. Both of these two kinds of Ganoderma lucidum polysaccharides reduced cell viability of cancer cell HepG2 in a dose and time-dependent manner. At low concentrations,there was no significant difference in the effectiveness of L1 and L2;while at concentrations over 0.8 μg/mL,the difference in the effectiveness of L2 in comparison to L1 became significant. At the concentrations of 3.2 μg/mL,the cancer cells were almost killed in 2 d.
基金the National Natural Science Foundation of China(No.50272047)Ministry of Education of China(No.704034)
文摘The spores of Ganoderma lucidum were ground and broken to ultrafine particles by high speed centrifugal shearing(HSCS) pulverizer. The characteristics of Ganoderma lucidum spores were analyzed by scanning electron microscope (SEM), Fourier transform infrared spectrophotometry (FTIR). Ultraviolet-visible pectrophotometer was used to determine the extraction ratio of aqueous solubility polysaccharide between the raw and broken spores. The immunological function on the mice before and after the breaking of spores wan investigated. The experimental results show that after being ground, the sporoderm-broken ratio reachs 100%, the original active ingredients of ganoderma lucidum spores do not change, and the extraction ratio of aqueous solubility polysaccharide is greatly increased by 40.08%. The broken spores show much higher immunological activity comparing with original spores of Ganoderma lucidum.