Effect of gastric fluid on adsorption and desorption of endocrine disrupting chemicals on microplastics

Microplastics and endocrine disrupting chemicals are emerging pollutants in the marine environment because of their potential hazards.The effect of gastric fluid on the adsorption and desorption of 17β-estradiol(E2)and 17α-ethynylestradiol(EE2)to microplastics was investigated.The adsorption and desorption isotherms of E2/EE2 on microplastics could be well fitted by the Freundlich model while the Gibbs free energy of these processes were negative,proving that the reaction occurred spontaneously on the heterogeneous surface of the microplastics.Desorption ratios of EE2(55%–59%)on PVC were larger than that of E2(49%–55%)to indicate that EE2 was less stable in gastric fluid,which could be explained by the fact that the hydrophobicity of EE2 was greater than E2.E2/EE2 were more easily desorbed from PVC in the gastric fluid and the desorption amount(5.25–12.91/7.19–17.86μg/g)increased by 2.51 times in comparison with that in saline solution(2.22–7.81/2.87–10.80μg/g).The decrease of pH and the increase of ionic strength in gastric fluid could further strengthen desorption of E2/EE2 from PVC.The promotion of gastric juice on desorption of PVC was achieved by reducing the hydrophobicity of the PVC surface.The desorption rate of E2/EE2 at 18°C and 38°C was respectively 44%–47%/46%–50%and 49%–55%/56%–59%,indicating that PVC loaded with E2/EE2 had a relatively greater risk of releasing pollutants in the gastric fluid of constant temperature marine organisms while higher temperatures exposed higher hazards for variable temperature animals.The interaction between microplastics and pollutants might be mainly hydrophobic interaction.

Aberrant gene methylation in the peritoneal fluid is a risk factor predicting peritoneal recurrence in gastric cancer

AIM:To investigate whether gene methylation in the peritoneal fluid (PF) predicts peritoneal recurrence in gastric cancer patients.METHODS: The gene methylation of CHFR (checkpoint with forkhead and ring finger domains), p16, RUNX3 (runt-related transcription factor 3), E-cadherin, hMLH1 (mutL homolog 1), ABCG2 (ATP-binding cassette, sub-family G, member 2) and BNIP3 (BCL2/adenovirus E1B 19 kDa interacting protein 3) were analyzed in 80 specimens of PF by quantitative methylation-specific polymerase chain reaction (PCR). Eighty patients were divided into 3 groups; Group A (n=35):the depth of cancer invasion was less than the muscularis propria; Group B (n=31): the depth of cancer invasion was beyond the muscularis propria. Both group A and B were diagnosed as no cancer cells in peritoneal cytology and histology; Group C (n=14): disseminated nodule was histologically diagnosed or cancer cells were cytologically defi ned in the peritoneal cavity.RESULTS: The positive rates of methylation in CHFR, E-cadherin and BNIP3 were significantly different among the 3 groups and increased in order of group A, B and C (0%,0% and 21% in CHFR, P<0.05; 20%, 45% and 50% in E-cadherin, P<0.05;26%,35% and 71% in BNIP3, P<0.05). In addition, the multigene methylation rate among CHFR, E-cadherin and BNIP3 was correlated with group A, B and C (9%,19% and 57%, P<0.001). Moreover, the prognosis was analyzed in group B, excluding 3 patients who underwent a non-curative resection. Two of the 5 patients with multigene methylation showed peritoneal recurrence after surgery, while those without or with a single gene methylation did not experience recurrence (P<0.05).CONCLUSION: This study suggested that gene methylation in the PF could detect occult neoplastic cells in the peritoneum and might be a risk factor for peritoneal metastasis.

模拟胃液环境下谷维素清除亚硝酸钠及阻断亚硝胺的作用

为降低人体内亚硝胺类化合物含量,采用模拟胃液结合液质联用的方法研究谷维素清除亚硝酸钠及阻断亚硝胺的作用。结果表明:在模拟胃液条件下,谷维素能有效清除亚硝酸钠,且亚硝酸钠的清除率与谷维素液添加量呈正相关关系;在膳食样品中添加谷维素能有效降低N-亚硝基-N-乙基苯胺、N-亚硝基二乙基胺、N-亚硝基吡咯烷的含量,其阻断率分别为100%、100%和24.4%。谷维素在模拟胃液条件下能通过降低亚硝酸钠含量减少内源性亚硝胺合成的反应底物,从而阻断亚硝胺的合成。

模拟胃液结合液质联用研究紫苏对内源性亚硝胺的阻断作用

为研究蔬菜对亚硝胺的阻断效果,在模拟胃液环境下结合液质联用,研究紫苏提取液对内源性亚硝胺的阻断作用。结果表明,在模拟胃液环境下加入紫苏液与亚硝酸钠,亚硝酸钠清除率可达10.4%。加入紫苏液、亚硝酸钠和二乙胺进行液相色谱质谱检测,空白样品和添加紫苏的样品中二乙基亚硝胺生成量分别为0.2、0.1 ng/mL,抑制率达到50%。添加紫苏的膳食中N-亚硝基二甲基(NDMA)、N-亚硝基二乙基(NDEA)、N-亚硝基二丁基(NDBA)、N-亚硝基吡咯烷(NPYR)含量显著降低。NDMA、NDEA在空白膳食中含量均为0.2 ng/g,紫苏试验组均未检出。NDBA空白对照为0.4 ng/g,紫苏试验组为0.2 ng/g,抑制率为50%;NPYR空白对照为8.6 ng/g,紫苏试验组为4.9 ng/g,抑制率为43%。因此,紫苏对内源性亚硝胺有明显的阻断作用。

围术期目标导向液体治疗对老年胃癌根治术伴高血压患者血流动力学、术后恢复及并发症的影响

目的探讨围术期目标导向液体治疗(GDFT)对老年胃癌根治术伴高血压患者血流动力学、术后恢复及并发症的影响。方法选取88例老年胃癌根治术伴高血压患者作为研究对象,采用随机数字表法分为治疗组和对照组,每组44例。治疗组围术期采用GDFT,对照组围术期采用常规液体治疗。比较2组患者不同时点的平均动脉压、心率、中心静脉血氧饱和度(ScvO_(2)),以及术后排气时间、术后住院时间、术后并发症发生率。结果2组患者平均动脉压、心率、ScvO_(2)的时间效应(F=60.488、49.789、39.423)、组间效应(F=17.317、12.969、10.101)及交互效应(F=26.415、22.546、17.294)比较,差异均有统计学意义(P<0.05);术毕,治疗组的平均动脉压、心率、ScvO_(2)高于麻醉前,且高于对照组,差异有统计学意义(P<0.05)。治疗组术后排气时间、住院时间依次为(2.25±0.61)、(10.29±1.96)d,分别短于对照组的(2.82±0.71)、(12.36±2.29)d,差异有统计学意义(P<0.05)。治疗组术后并发症总发生率为11.36%,低于对照组的29.54%,差异有统计学意义(P<0.05)。结论将GDFT应用于老年胃癌根治术伴高血压患者围术期,可提供更稳定的血流动力学环境,改善组织灌注情况,加速术后康复进程,并减少术后并发症。

乳酸乳球菌的单菌包埋技术及在胃肠道环境中的性能评价

由于益生菌对消化系统的强酸和高浓度胆汁盐环境较为敏感,并且在肠道中定殖能力较低,因此开发新颖有效的益生菌包埋体系用于解决上述问题很有必要。本研究使用羧甲基化β-葡聚糖(m GN),通过金属-酚醛网络结构(Fe-TA)的桥联作用,将其黏附在乳酸乳球菌(LL)表面,构建乳酸乳球菌的包埋体系LL@Fe-TA@mGN,并评价LL@Fe-TA@mGN对胃液与胆盐的抗性及其在体内的滞留能力。结果表明:当mGN的质量浓度为0.12 mg/mL时,LL@Fe-TA@mGN体系的粒径和zeta-电位都达到峰值,表明该质量浓度为mGN包埋单个乳酸乳球菌的最佳质量浓度。在MRS液体培养基中,LL@FeTA@mGN的生长曲线与LL的生长曲线没有显著性差异,说明mGN的毒性可以忽略不计。在扫描电镜与透射电镜分析中均可清晰地看到一层“膜”完整地包覆在乳酸乳球菌表面。经2 h模拟胃液与胆盐的孵育,mGN包埋后的LL抗胃液能力与抗胆盐能力相比于未包埋的LL分别提升14.63倍与1.94倍。体内荧光成像实验证实LL@Fe-TA@mGN具有更强的肠道滞留能力。综上,本研究开发的乳酸乳球菌单菌包埋技术能够显著提升乳酸乳球菌的胃肠道抗性与体内滞留能力。研究结果可为益生菌包埋系统的开发与利用提供新的思路和策略。

响应面法探究纳豆激酶活性保留条件及肠溶性胶囊的保护作用

采用纤维蛋白降解法测定活性,响应面法分析纳豆激酶在不同因素中活性变化,明确纳豆激酶最优活性保留条件。采用胃液-肠液串联式方法,比较普通纳豆激酶胶囊与肠溶性胶囊在人工胃肠液中的稳定性,为开发肠溶性纳豆激酶胶囊做理论支撑。结果表明纳豆激酶活性保留最优条件为:PH=8、转速为40 r/min、时间为1.3 h、温度为23℃。此时纳豆激酶活性保留为20291.7 FU/g。在人工胃液处理后,活性下降到30.9%。普通胶囊与肠溶性胶囊在人工胃肠液处理后活性分别为28.0%、53.1%。最终结论为纳豆激酶在酸性的胃液中极不稳定,活性丧失巨大。在人工肠液中酶较稳定。肠溶性胶囊与普通胶囊相比具有保护作用,将纳豆激酶制成肠溶性的产品服用,能使得纳豆激酶更加稳定、活性更高。

胃溃疡出血患者胃液PH、EGF、PGE_(2)和胃动素水平与其消化内镜治疗效果及胃肠功能的关系

目的 探讨胃溃疡出血患者胃液PH、表皮生长因子(EGF)和前列腺素E_(2)(PGE_(2))水平与其消化内镜治疗效果及胃肠功能的关系。方法 选取2022年1月至2024年5月于武汉大学中南医院行消化内镜治疗的胃溃疡出血患者67例。对患者消化内镜治疗时收集的胃液中PH、EGF和PGE_(2)水平进行检测。评价患者的疾病治疗有效率。比较不同疗效患者的胃液PH、EGF和PGE_(2)水平,并采用ROC曲线下面积评价其胃液PH、EGF和PGE_(2)水平对消化内镜治疗效果的早期评估效能。收集患者术后肠鸣音恢复时间、肛门排气时间、止血时间、住院时间、溃疡愈合时间等胃肠功能和恢复指标,并通过Pearson相关法分析其胃液PH、EGF和PGE_(2)水平与术后胃肠功能和恢复指标的关系。结果 胃溃疡出血患者67例的临床治疗有效率为71.64%(48/67)。与治疗有效患者比较,治疗无效患者的胃液PH、EGF和PGE_(2)水平均较低(P<0.05)。ROC曲线分析结果显示,胃液PH、EGF和PGE_(2)水平对消化内镜治疗效果均具备一定的预测价值,以三者联合检测的早期评估效能最佳。患者术后肠鸣音恢复时间、肛门排气时间、止血时间、住院时间、溃疡愈合时间分别为(7.16±2.38)min、(26.96±6.41)h、(20.66±4.37)h、(4.52±1.16)d和(23.14±4.18)d。Pearson相关分析结果显示,胃溃疡出血患者胃液PH、EGF和PGE_(2)水平与其术后肠鸣音恢复时间、肛门排气时间、止血时间、住院时间、溃疡愈合时间等胃肠功能和恢复指标均呈负相关(P<0.05)。结论 胃溃疡出血患者胃液PH、EGF和PGE_(2)水平与其消化内镜治疗效果、术后胃肠功能和恢复情况均相关,可作为胃溃疡出血内镜治疗患者疗效的早期评估参考指标。

基于利福平耐药结核分枝杆菌半巢式实时荧光定量PCR技术检测胃液结核分枝杆菌在儿童肺结核中的诊断价值

目的分析基于利福平耐药结核分枝杆菌半巢式实时荧光定量PCR技术(Xpert-MTB/RIF)检测胃液结核分枝杆菌在儿童肺结核中的诊断价值。方法选取2020年1月到2021年12月贵阳市公共卫生救治中心儿童结核科收治50例疑似肺结核患儿作为研究对象,取所有患儿晨起空腹胃液,分别行抗酸染色涂片、Xpert-MTB/RIF及罗氏(L-J)培养检测。以MTB培养作为金标准,分析不同检测方法结果,比较不同检测方法对儿童肺结核的诊断效能,分析Xpert-MTB/RIF及L-J培养阳性患儿MTB利福平耐药性情况及Xpert-MTB/RIF检测法与L-J培养法的药敏试验一致性。结果50例疑似肺结核患儿中,MTB培养结果检出36例肺结核,肺炎14例。抗酸染色涂片法检出阳性1例,阴性49例;L-J培养法检出阳性11例,阴性39例;Xpert MTB/RIF检测法检出阳性18例,阴性32例。Xpert MTB/RIF检测法、L-J培养法对儿童肺结核的诊断灵敏度高于抗酸染色涂片检测法,差异有统计学意义(P<0.05),Xpert MTB/RIF检测与L-J培养法诊断灵敏度比较差异无统计学意义。不同检测方法对儿童肺结核的诊断特异度、阳性预测值、阴性预测值比较差异均无统计学意义。Xpert MTB/RIF检测MTB对利福平的耐药敏感结果与L-J培养法的一致性Kappa值为0.891,具有较高的一致性。结论Xpert MTB/RIF检测较方便与快捷,具有较高的诊断效能,其检测MTB对利福平的药敏试验结果与L-J培养法一致性较高,有利于临床及早诊断儿童肺结核疾病并进行干预指导。

王彦刚基于“核心病机观”从“浊毒”论治胃癌前病变

王彦刚教授承古拓今提出了疾病的“核心病机观”理论,他认为浊毒内蕴脾胃为贯穿于胃癌前病变之始末的“核心病机”,浊毒胶结不解是胃癌前病变病情进展之病机关键,也是其迁延不愈、多阶段发展的重要病理因素。在治疗上,以化浊解毒为根本大法,选用自拟化浊解毒方为主要方药,随证行以清热、祛湿、行气、化积、宣郁、健脾、益胃等治法,使热邪清、浊毒化、清阳升、浊阴降,痰化瘀除,脾升胃降,气机通畅,及时阻断病情发展甚至逆转胃癌前病变,收效显著,颇具临床指导意义。

Stability of Xuesaitong capsules in gastrointestinal lavage fluid

Objective:To obtain a formulation with high bioavailability through evaluation of the stability of three types of Xuesaitong capsules in the stomachs and intestines of rats.We compared the stability of the Panax notoginsenoside R1 as well as the ginsenosides Rg1,Rb1,Re,and Rd in different formulations.Methods:Artificial stomach fluid(ASF)and artificial intestinal fluid(AIF)were prepared.Stability of three types of Xuesaitong capsules was examined for 4 h in stomachs and 24 h in intestines.Samples were analyzed at different times by high-performance liquid chromatography.Percent content of NGR1,GRg1,GRb1,GRe,and GRd at different times was calculated.Results:Hard capsules incubated in ASF disintegrated within 2e3 min,whereas soft capsules disintegrated within 7e8 min.Components in hard capsules were dissolved rapidly in water,with content of each compound reaching 90%in 5 min,and degradation of each compound reaching 30e50%after incubation for 240 min.Dissolution and degradation of each component in soft capsules with a water-soluble base tended to balance at 30 e90 min.Contents in soft capsules with a lipid-soluble base showed slow dissolution after ASF incubation for 120 min.Five saponins in identical types of capsules incubated in ASF had similar stability curves.Contents of hard capsules and soft capsules with a watersoluble base degraded rapidly within 30 min and reached a plateau when Xuesaitong capsules were incubated in AIF.

Role of RECK methylation in gastric cancer and its clinical significance

AIM:To investigate the relation between RECK methylation and clinicopathological characteristics of gastric cancer patients and evaluate the role of RECK methylation in peritoneal metastasis of gastric cancer.METHODS:Methylation of RECK gene in 40 paired samples of gastric cancer and its corresponding adjacent normal mucosa,lymph nodes and peritoneal irrigation fluid was detected by methylation-specific polymerase chain reaction.RESULTS:Aberrant methylation of RECK gene was detected in 27.5%(11/40)of the adjacent normal mucosa samples,in 47.5%(19/40)of gastric cancer samples,in 57.1%(12/21)of the lymph node samples,and in 35%(14/40)of peritoneal irrigation fluid samples,respectively,with a significant difference between the adjacent normal mucosa and lymph node samples(P=0.023).Presence of RECK methylation in the primary tumor samples was significantly correlated with tumor invasion(P=0.023).The accuracy of RECK methylation in peritoneal lavage fluid samples for the diagnosis of peritoneal metastasis of gastric cancer was 72.5%(26/40),with a sensitivity of 66.7%(6/9) and a specificity of 74.2%(23/31).CONCLUSION:Aberrant methylation of RECK gene may provide useful information for the early diagnosis and treatment of peritoneal metastasis of gastric cancer.

Promoter Hypermethylation of KiSS-1 Gene in Gastric Cancer

Objective： To investigate the association between KiSS-1 methylation and clinicopathological characteristics of gastric cancer and evaluate the role of peritoneal lavage fluid in detecting peritoneal metastases. Methods： The methylation status of KiSS-1 gene in 40 gastric cancer specimens, the corresponding adjacent normal mucosa, lymph nodes and peritoneal lavage fluid was investigated by methylation-spcific polymerase chain reaction（MS-PCR）. Results： Aberrant methylation of KiSS-1 gene was detected in 55%（22/40） of the adjacent normal mucosa, 82.5% （33/40）of gastric cancer specimens, 80.95%（17/21） of the lymph nodes, and 42.5%（17/40） of peritoneal lavage fluid. Methylation in gastric carcinoma and lymphonode was more frequent than in non-neoplastic gastiric mucosa. Presence of KiSS-1 methylation in peritoneal lavage fluid was significantly correlated with tumor invasion （P=0.043）. The accuracy of KiSS-1 methylation in peritoneal lavage fluid for diagnosing peritoneal metastasis was 70%, with a sensitivity of 77.8% and a specificity of 67.7%. Conclusion： Aberrant methylation of KiSS-1 gene is a common event in the occurrence and progression of gastric carcinoma, which may provide useful information for the early diagnosis of peritoneal metastases and a new therapy for gastric cancer.

以胃液pH值为导向的颅脑损伤应激相关性黏膜病变预防策略及其对相关临床事件的影响

目的探讨以胃液pH值为导向的颅脑损伤应激相关性黏膜病变(SRMD)预防策略及其对相关临床事件的影响。方法将72例颅脑损伤患者随机分为对照组35例与观察组37例。给予对照组患者常规静脉注射质子泵抑制剂(PPI)预防SRMD,给予观察组患者以胃液pH值为导向的预防策略。比较两组患者住院期间SRMD、医院获得性肺炎(HAP)、艰难梭菌感染(CDI)、呼吸衰竭、休克、肾功能受损及死亡的发生率。结果两组患者的SRMD、肾功能受损及死亡发生率比较,差异均无统计学意义(均P>0.05)。观察组HAP、CDI、呼吸衰竭、休克的发生率均低于对照组(均P<0.05)。结论相比于常规的PPI预防策略,采用以胃液pH值为导向的预防策略,可在保证SRMD预防效果的同时,降低颅脑损伤患者的HAP、CDI、呼吸衰竭、休克发生率,安全性较高。

牛蒡燕窝肽复合饮料制备及体外模拟消化特性研究

以牛蒡、经酶解处理后的燕窝为主料,银耳、梨为辅料,研制功能性复合饮料。以感官评价为指标,采用响应面试验优化配方工艺。得到最佳配方为牛蒡浸提液添加量30%、燕窝酶解液20%、银耳浸提液15%、梨浸提液35%和白砂糖11%。采用体外模拟该复合饮料胃肠消化,对其唾液酸、绿原酸、蛋白质、多肽、氨基酸含量和自由基清除率等进行测定。结果发现,消化后抗氧化性和大分子活性成分含量均降低,氨基酸含量明显增加。

人工胃/肠液和大鼠肠道菌群对氟苯尼考磺胺噻唑稳定性的影响

为新型偶联化合物-氟苯尼考磺胺噻唑(FST)研制和开发口服制剂提供数据支持,采用高效液相色谱法,对FST在5种溶液介质(无蛋白酶人工肠液、人工肠液、无蛋白酶人工胃液、人工胃液及大鼠肠道菌群培养液)中不同时刻的含量进行了研究,判断其稳定性差异。结果表明,FST在人工胃液和无蛋白酶人工胃液0~6 h含量保持基本稳定;在人工肠液和无蛋白酶人工肠液中孵育30 min已完全降解,且新出现的色谱峰在相同色谱条件下与氟苯尼考和磺胺噻唑对照品出峰时间相同。由此可知,FST的代谢产物为氟苯尼考和磺胺噻唑;FST在肠道菌群中发生了降解,0~8 h,FST含量迅速下降,而8~12 h趋于平稳,降至50%。说明FST在人工肠液中有明显的降解,在人工胃液中趋于稳定,肠道菌群对其稳定性也有一定的影响。

三氯生在体外模拟胃肠液中的降解动力学

研究不同初始质量浓度的三氯生在模拟胃液和肠液中的消化动力学。结果表明,三氯生在模拟胃液和肠液中反应3 h后均不能被完全消化,低、中、高初始质量浓度的三氯生在胃液中的最终残留率分别为17.40%、32.02%和39.54%,在肠液中的最终残留率分别为33.32%、43.10%和51.26%。三氯生在模拟胃液中的消化率高于肠液,且消化过程与进入人体的初始质量浓度相关,低初始质量浓度组的消化率最高,高质量浓度组的最低。一级反应动力学模型可以较好地描述三氯生在模拟胃肠液中的降解过程和降解速率,模型的线性相关系数R大于0.9。三氯生在胃肠液中的消化特征呈现先快后慢,后期趋于稳定的状态。总体上,三氯生在模拟胃液和肠液中较难被完全消化。

动态监测胃液pH值对指导重症脑卒中患者质子泵抑制剂使用的价值

目的:分析动态监测胃液pH值对指导重症脑卒中患者质子泵抑制剂使用的价值。方法:选取2020年1月—2021年2月金乡县人民医院收治的重症脑卒中患者68例作为研究对象,采取随机数字表法分为对照组与观察组,各34例。对照组进行早期肠内营养支持3 d后,停止使用质子泵抑制剂;观察组持续使用质子泵抑制剂联合早期肠内营养支持治疗,两组均在实施肠内营养支持治疗第4天动态监测胃液pH值。比较两组胃液pH值在不同阶段的变化情况及临床相关指标。结果:观察组鼻饲后0.5、1 h胃液平均pH值及中位pH值均高于对照组,差异有统计学意义(P<0.05)。两组应激溃疡出血率、治疗好转率、病死率比较,差异无统计学意义(P>0.05)。结论:动态监测胃液pH值能够明确质子泵抑制剂应用剂量、使用时间及早期肠内营养支持的最佳时机,对提高重症脑卒中患者的临床疗效、改善其预后有积极作用。

辐照处理对蟹类过敏原(原肌球蛋白)性质的影响

食物过敏是当前食品安全领域较为突出的问题，辐照作为食物过敏原的加工处理方法之一,引起了学者的重视．作者对中华绒鳌蟹和三疣梭子蟹的主要过敏原（原肌球蛋白）进行了辐照处理，并分析了辐照处理对原肌球蛋白性质的影响．十二烷基磺酸钠-聚丙烯酰胺凝胶电泳结果显示，部分纯化的蟹类原肌球蛋白经7kGy辐照处理后出现明显降解,10kGy辐照处理后完全降解；蟹类过敏者血清的免疫印迹分析结果显示．部分纯化原肌球蛋白经3～7kGy辐照处理后的过敏原性无明显变化．而10kGy辐照处理后的过敏原性降低；兔抗锯缘青蟹原肌球蛋白抗体的免疫胶体金层析检测结果证实部分纯化原肌球蛋白经10kGy辐照处理后的抗原性为阴性．但是．3～10kGy辐照处理对整蟹原肌球蛋白及其过敏原性无明显影响。模拟胃液消化反应结果也表明3～10kGy辐照处理对整蟹原肌球蛋白的酶解消化特性无明显影响．

日本鳗鲡胶原蛋白和小清蛋白的过敏原性

以日本鳗鲡皮与肌肉组织为研究对象,采用碱溶、酸溶、盐析、冻干等方法纯化得到胶原蛋白,采用加热、饱和硫酸铵分级盐析、DEAE-Sepharose阴离子交换层析等方法纯化得到两种亚型的小清蛋白(PV-Ⅰ和PV-Ⅱ),纯化的目标蛋白经动物特异性抗体的免疫印迹实验确证。酶联免疫吸附测定和免疫印迹分析结果显示,纯化的胶原蛋白和小清蛋白分别与鱼类过敏患者阳性血清发生特异性反应,且二者之间无免疫交叉反应。体外模拟胃液消化实验和SDS-PAGE分析结果显示,胶原蛋白和小清蛋白均具有较高的消化稳定性。结果提示,日本鳗胶原蛋白和小清蛋白具有较高的消化稳定性和免疫原性,二者可引发不同患者的IgE介导特异性超敏反应。