Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds

Investigating the pathophysiological mechanisms underlying brain disorders is a priority if novel therapeutic strategies are to be developed. In vivo studies of animal models and in vitro studies of cell lines/primary cell cultures may provide useful tools to study certain aspects of brain disorders. However, discrepancies among these studies or unsuccessful translation from animal/cell studies to human/clinical studies often occur, because these models generally represent only some symptoms of a neuropsychiatric disorder rather than the complete disorder. Human brain slice cultures from postmortem tissue or resected tissue from operations have shown that, in vitro, neurons and glia can stay alive for long periods of time, while their morphological and physiological characteristics, and their ability to respond to experimental manipulations are maintained. Human brain slices can thus provide a close representation of neuronal networks in vivo, be a valuable tool for investigation of the basis of neuropsychiatric disorders, and provide a platform for the evaluation of novel pharmacological treatments of human brain diseases.A brain bank needs to provide the necessary infrastructure to bring together donors, hospitals, and researchers who want to investigate human brain slices in cultures of clinically and neuropathologically well-documented material.

边缘检测算子在壁虎脑切片显微图像中的研究

针对壁虎脑切片显微图像的边缘检测需要,分析几种常用算子基本原理及算法实现,用VC++6.0工具编程实现各种算子对一幅大壁虎脑组织切片显微图像进行边缘检测。对实验结果进行分析得出结论,Canny算子检测出的边缘比一般微分算子更精确、虚假边缘少,但也可能平滑掉一些有用的边缘和细节,综合考虑比较适合该类图像的边缘检测。