This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca ch...This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites,account for 9.87% of wholly GenBank database.Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites,accounting for 53.33% of the total microsatellite-containing sequences;11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences,accounting for 36.66% and 10.01% respective.17 primer pairs were designed from these microsatellite sequences.Among the 17 primer pairs,13 pairs have amplified products,6 pairs can achieve clear PCR products by Electrophoresis.Genotype analysis results indicated that:the average polymorphism information contents(PIC)were 0.692(ranged:0.567-0.806),were all polymorphic loci.展开更多
美国国立生物技术信息中心(NCBI)隶属于美国国立卫生研究院(National Institute of Health,NIH),是国家医学图书馆(National Library of Medicine,NLM)的一个分支机构,负责通过INTERNET等形式为医学、生物学方面的基础研究提供新的信息...美国国立生物技术信息中心(NCBI)隶属于美国国立卫生研究院(National Institute of Health,NIH),是国家医学图书馆(National Library of Medicine,NLM)的一个分支机构,负责通过INTERNET等形式为医学、生物学方面的基础研究提供新的信息。本文主要介绍NCBI网中GENBANK序列数据库的功能及使用方法,如:数据的检索查询,数据递交等;同时还简单介绍了生物医学文献数据库(PreMedline)以及NCBI所能提供的其他服务。NCBI的网址是www.nchi.nlm.nih.展开更多
文摘This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats(SSRs),or microsatellites.A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites,account for 9.87% of wholly GenBank database.Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites,accounting for 53.33% of the total microsatellite-containing sequences;11 trinucleotide repeats and 3 tetranucleotide repeats were found in these microsatellite sequences,accounting for 36.66% and 10.01% respective.17 primer pairs were designed from these microsatellite sequences.Among the 17 primer pairs,13 pairs have amplified products,6 pairs can achieve clear PCR products by Electrophoresis.Genotype analysis results indicated that:the average polymorphism information contents(PIC)were 0.692(ranged:0.567-0.806),were all polymorphic loci.
文摘美国国立生物技术信息中心(NCBI)隶属于美国国立卫生研究院(National Institute of Health,NIH),是国家医学图书馆(National Library of Medicine,NLM)的一个分支机构,负责通过INTERNET等形式为医学、生物学方面的基础研究提供新的信息。本文主要介绍NCBI网中GENBANK序列数据库的功能及使用方法,如:数据的检索查询,数据递交等;同时还简单介绍了生物医学文献数据库(PreMedline)以及NCBI所能提供的其他服务。NCBI的网址是www.nchi.nlm.nih.
