Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF4...Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF436066). Th e le ngth of this ORF is 228bp, encoding a protein with Mr of 8.56 kD and isoelectri c point of 6.56. Multiple sequence alignment indicated that S.litura UBI is very similar to the homologous proteins of other eukaryotic species and it has 84% id entity with S.litura nucleopolyhedrovirus (SpltMNPV) UBI at amino acid level . RT -PCR analysis showed that S.litura UBI gene is ubiquitously expressed in la rva t issues which are susceptible to SpltMNPV infection. By constructing E.coli e xpre ssion vector, S.litura UBI was highly expressed and the recombinant protein was purified using Ni-NTA resin column, and currently further study on the function of S.litura UBI in SpltMNPV infection is underway.展开更多
文摘Ubiquitin (UBI) plays a very important role in regulated non-lysosoma l ATP dependent protein degradation. In the present work, the coding sequence of Spodoptera litura UBI gene was isolated (GenBank Accession No. AF436066). Th e le ngth of this ORF is 228bp, encoding a protein with Mr of 8.56 kD and isoelectri c point of 6.56. Multiple sequence alignment indicated that S.litura UBI is very similar to the homologous proteins of other eukaryotic species and it has 84% id entity with S.litura nucleopolyhedrovirus (SpltMNPV) UBI at amino acid level . RT -PCR analysis showed that S.litura UBI gene is ubiquitously expressed in la rva t issues which are susceptible to SpltMNPV infection. By constructing E.coli e xpre ssion vector, S.litura UBI was highly expressed and the recombinant protein was purified using Ni-NTA resin column, and currently further study on the function of S.litura UBI in SpltMNPV infection is underway.
