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Genotyping ex situ trees of Abies nebrodensis translocated from the original Sicilian population to enrich the gene pool
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作者 Anna De Rogatis Fulvio Ducci +2 位作者 Serena Guerri Angela Teani Roberta Proietti 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第4期1095-1106,共12页
As a dynamic ex situ conservation strategy,a clonal seed orchard was started in a nursery in Pomaio(POM)in central Italy in 1993 for an assisted migration experiment of Abies nebrodensis(Lojac.)Mattei.Two artifi cial ... As a dynamic ex situ conservation strategy,a clonal seed orchard was started in a nursery in Pomaio(POM)in central Italy in 1993 for an assisted migration experiment of Abies nebrodensis(Lojac.)Mattei.Two artifi cial ex situ populations were planted with this gene pool:a seedling arboretum in Pieve Santo Stefano(PSS)and a small dendrological collection in Papiano(PAP),both originating from the Sicilian relict population.Here,using AFLP markers,we estimated the relatedness among the relocated genotypes of the three collections to check whether the three collections had suffi cient genetic variability to be considered as additional sources of variability to the original gene pool for the assisted migration strategy.High individual genetic variability was found in the collections;each plant had a diff erent genotype and was confi rmed to belong to its population of origin.PAP and PSS trees were shown to be only from the original population of A.nebrodensis species and were derived from a limited set of maternal fertile genotypes.Based on the Sicilian fi r population inventory,nursery production in Sicily,and structure clustering analysis,close genetic relationships among POM,PAP and several PSS trees(1–35)were evident.Similarly,the PSS group(36–78)was genetically close to tree 1 of POM and in a lesser proportion to plants 7 and 9 of POM.The sampling of seedlings used to form batches in the nursery might have infl uenced the structure of the resultant plantations.All genotypes will be useful for enriching the original gene pool. 展开更多
关键词 Sicilian fir GENOTYPING Ex situ conservation gene pool Assisted migration
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Genomic insights into the origin and evolution of spelt(Triticum spelta L.)as a valuable gene pool for modern wheat breeding
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作者 Yongfa Wang Zihao Wang +10 位作者 Yongming Chen Tianyu Lan Xiaobo Wang Gang Liu Mingming Xin Zhaorong Hu Yingyin Yao Zhongfu Ni Qixin Sun Weilong Guo Huiru Peng 《Plant Communications》 SCIE CSCD 2024年第5期75-90,共16页
Spelt(Triticum aestivum ssp.spelta)is an important wheat subspecies mainly cultivated in Europe before the 20th century that has contributed to modern wheat breeding as a valuable genetic resource.However,relatively l... Spelt(Triticum aestivum ssp.spelta)is an important wheat subspecies mainly cultivated in Europe before the 20th century that has contributed to modern wheat breeding as a valuable genetic resource.However,relatively little is known about the origins and maintenance of spelt populations.Here,using resequencing data from 416 worldwide wheat accessions,including representative spelt wheat,we demonstrate that Eu-ropean spelt emerged when primitive hexaploid wheat spread to the west and hybridized with pre-settled domesticated emmer,the putative maternal donor.Genomic introgression regions from domesticated emmer confer spelt’s primitive morphological characters used for species taxonomy,such as tenacious glumes and laterflowering.We propose a haplotype-based"spelt index"to identify spelt-type wheat vari-eties and to quantify utilization of the spelt gene pool in modern wheat cultivars.This study reveals the ge-netic basis for the establishment of the spelt wheat subspecies in a specific ecological niche and the vital role of the spelt gene pool as a unique germplasm resource in modern wheat breeding. 展开更多
关键词 WHEAT SPELT interspecific hybridization gene pool modern wheat breeding
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A Gene-Pool Based Genetic Algorithm for TSP 被引量:6
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作者 Yang Hui, Kang Li-shan, Chen Yu-pingState Key Laboratory of Software Engineering, Wuhan University, Wuhan 430072, Hubei, China 《Wuhan University Journal of Natural Sciences》 CAS 2003年第S1期217-223,共7页
Based on the analysis of previous genetic algorithms (GAs) for TSP, a novel method called Ge- GA is proposed. It combines gene pool and GA so as to direct the evolution of the whole population. The core of Ge- GA is t... Based on the analysis of previous genetic algorithms (GAs) for TSP, a novel method called Ge- GA is proposed. It combines gene pool and GA so as to direct the evolution of the whole population. The core of Ge- GA is the construction of gene pool and how to apply it to GA. Different from standard GAs, Ge- GA aims to enhance the ability of exploration and exploitation by incorporating global search with local search. On one hand a local search called Ge- Lo-calSearch operator is proposed to improve the solution quality, on the other hand the modified Inver-Over operator called Ge InverOver is considered as a global search mechanism to expand solution space of local minimal. Both of these operators are based on the gene pool. Our algorithm is applied to 11 well-known traveling salesman problems whose numbers of cities are from 70 to 1577 cities. The experiments results indicate that Ge- GA has great robustness for TSP. For each test instance, the average value of solution quality, found in accepted time, stays within 0. 001% from the optimum. 展开更多
关键词 genetic Algorithm gene pool minimal spanning tree combinatorial optimization TSP
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Hv-SEMIGROUP STRUCTURE ON F2-OFFSPRING OF A GENE POOL 被引量:1
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作者 M. GHADIRI B. DAVVAZ R. NEKOUIAN 《International Journal of Biomathematics》 2012年第4期1-13,共13页
Mendel, the father of genetics took the first steps in defining "contrasting characters, genotypes in F1 and F2... and setting different laws". The genotypes of F2 is dependent on the type of its parents genotype an... Mendel, the father of genetics took the first steps in defining "contrasting characters, genotypes in F1 and F2... and setting different laws". The genotypes of F2 is dependent on the type of its parents genotype and it follows certain roles. Purpose of this paper is to analyze the second generation genotypes of monohybrid and a dihybrid with a mathematical structure. We use the concept of Hv-semigroup structure in the F2-genotypes with cross oper- ation and prove that this is an Hv-semigroup. We determine the kinds of number of the Hv-subsemigroups of F2-genotypes. 展开更多
关键词 Algebraic hyperstructure Hv-semigroup GENOTYPE OFFSPRING gene pool.
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Sequencing and Analysis of G HR Gene from Genomic DNA Pools of Mink
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作者 Rong Min Xu Jiaping +2 位作者 Tu Jianfeng Gao Feng Xing Xiumei 《Animal Husbandry and Feed Science》 CAS 2018年第3期166-167,205,共3页
In order to study the single nucleotide polymorphisms (SNPs) of growth hormone receptor (GHR) gene in mink populations, genomic DNA pools of Minghua black minks and silver-blue minks were constructed, and the 10^t... In order to study the single nucleotide polymorphisms (SNPs) of growth hormone receptor (GHR) gene in mink populations, genomic DNA pools of Minghua black minks and silver-blue minks were constructed, and the 10^th exon of GHR gene was PCR amplified from the two DNA pools and sequenced. The results showed that two SNPs were found at position 209 (T/C) and position 533 (C/A) of the 10^th exon of GHR gene in the two mink populations. 展开更多
关键词 MINK Growth hormone receptor (GHR) gene DNA pools SEQUENCING Single nucleotide polymorphisms (SNPs)
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Identification and Analysis of Genetic Diversity Structure Within Pisum Genus Based on Microsatellite Markers 被引量:4
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作者 ZONG Xu-xiao Rebecca Ford +2 位作者 Robert R Redden GUAN Jian-ping WANG Shu-min 《Agricultural Sciences in China》 CAS CSCD 2009年第3期257-267,共11页
To assesse the genetic diversity among wild and cultivated accessions of 8 taxonomic groups in 2 species, and 5 subspecies under Pisum genus, and to analyze population structure and their genetic relationships among v... To assesse the genetic diversity among wild and cultivated accessions of 8 taxonomic groups in 2 species, and 5 subspecies under Pisum genus, and to analyze population structure and their genetic relationships among various groups of taxonomy, the study tried to verify the fitness of traditionally botanical taxonomic system under Pisum genus and to provide essential information for the exploration and utilization of wild relatives of pea genetic resources. 197 Pisum accessions from 62 counties of 5 continents were employed for SSR analysis using 21 polymorphic primer pairs in this study. Except for cultivated field pea Pisum sativum ssp. sativum var. sativum (94 genotypes), also included were wild relative genotypes that were classified as belonging to P. fulvum, P. sativum ssp.abyssinicum, P. sativum ssp. asiaticum, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. elatius, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. arvense (103 genotypes). The PCA analyses and 3-dimension PCA graphs were conducted and drawn by NTSYSpc 2.2d statistical package. Nei78 genetic distances among groups of genetic resources were calculated, and cluster analysis using UPGMA method was carried out by using Popgene V1.32 statistical package, the dendrogram was drawn by MEGA3.1 statistical package. Allelic statistics were carried out by Popgene V1.32. The significance test between groups of genotypes was carried out by Fstat V2.9.3.2 statistical package. 104 polymorphic bands were amplified using 21 SSR primer pairs with unambiguous unique polymorphic bands. 4.95 alleles were detected by each SSR primer pair in average, of which 65.56% were effective alleles for diversity. PSAD270, PSAC58, PSAA18, PSAC75, PSAA175 and PSAB72 were the most effective SSR pairs. SSR alleles were uniformly distributed among botanical taxon units under Pisum genus, but significant difference appeared in most pairwise comparisons for genetic diversity between taxon unit based groups of genetic resources. Genetic diversity level of wild species P. fulvum was much lower than the cultivated species P. sativum. Under species P. sativum, P. sativum ssp. sativum var. sativum and P. sativum ssp. asiaticum were the highest in gentic diversity, followed by P. sativum ssp. elatius var. elatius and P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio, P. sativum ssp. sativum vat. arvense and P. sativum ssp. abyssinicum were the lowest. Four gene pool clusters were detected under Pisum genus by using PCA analysis. Gene pool "fulvum" mainly consisted of wild species Pisum fulvum, gene pool "abyssinicum" mainly consisted of P. sativum ssp. abyssinicum, and gene pool "arvense" mainly consisted of P. sativum ssp. sativum var. arvense. While gene pool "sativum" were composed by 5 botanical taxon units, they are P. sativum ssp. asiaticum, P. sativum ssp. elatius var. elatius, P. sativum ssp. transcaucasicum, P. sativum ssp. elatius var. pumilio and P. sativum ssp. sativum var. sativum. "sativum" gene pool constructed the primary gene pool of cultivated genetic resources; "fulvum" gene pool, "abyssinicum" gene pool and "arvense" gene pool together constructed the secondary gene pool of cultivated genetic resources. Pairwise Nei78 genetic distance among botanical taxon based groups of pea genetic resources ranged from 7.531 to 35.956, 3 large cluster groups were identified based on the UPGMA dendrogram. Group Ⅰ equals to "sativum" and "arvense" gene pools, Group Ⅱ equals to "abyssinicum" gene pool, and Group Ⅲ equals to "fulvum" gene pool. The UPGMA clustering results generally supporting the PCA clusting results. There were significant differences among most botanical groups under Pisum genus, with clear separation of four gene pools for genetic diversity structure. The research results partially support the traditional botanical taxonomy under Pisum genus, and pointed out its advantage and shortcoming. In order to broaden the genetic bases of pea varieties, the genetic potentials in the four gene pools should be thoroughly exploited. 展开更多
关键词 Pisum genus SSR genetic diversity botanical taxonomy gene pool
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基于混池测序及遗传图谱定位玉米雄穗分枝数基因
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作者 周步进 韦绍丽 +5 位作者 何静丹 覃嘉明 郑加兴 黄安霞 时成俏 王兵伟 《南方农业学报》 CAS CSCD 北大核心 2024年第5期1249-1258,共10页
【目的】构建玉米雄穗分枝数极端混池和分子遗传图谱,对雄穗分枝数进行QTL定位,为探究玉米雄穗分枝数的发育机制及选育雄穗分枝数少的品种提供理论参考。【方法】将雄穗分枝少的自交系SNM131与雄穗分枝多的自交系HY813组配F_(2)代群体,... 【目的】构建玉米雄穗分枝数极端混池和分子遗传图谱,对雄穗分枝数进行QTL定位,为探究玉米雄穗分枝数的发育机制及选育雄穗分枝数少的品种提供理论参考。【方法】将雄穗分枝少的自交系SNM131与雄穗分枝多的自交系HY813组配F_(2)代群体,统计群体各单株的雄穗分枝数,分析其遗传模式。从F_(2)代群体中分别挑选33个雄穗分株少的单株和38个雄穗分枝多的单株构建混池,对2个亲本和2个子代混池分别展开30×、17×和28×覆盖度的全基因组重测序,对测序数据进行关联分析,获得雄穗分枝数性状的定位区间。利用10K芯片对F_(2)代群体276个单株进行单核苷酸多态性(SNP)标记分型,所得数据用于构建遗传连锁图谱,并结合雄穗分枝数表型数据,利用全基因组复合区间作图法(GCIM)进行QTL定位。最后根据定位区间对应的B73参考基因组的物理区段上的基因注释信息,预测调控雄穗分枝数的候选基因。【结果】F_(2)代群体雄穗分枝数平均7.6个,F_(2)代群体在雄穗分枝数性状上偏向母本SNM131,以少雄穗分枝为主。全基因组重测序共获得1812886个SNP标记和24714个插入缺失标记(InDel),并基于混合分组分析法(BSA)进行雄穗分枝数性状分析,最终共获得6个显著关联区间,分布于玉米5、8和10号染色体。其中,10号染色体上一个峰值最高的关联区间(物理位置127.61~129.46 Mb),推测为控制雄穗分枝数性状的主效位点。构建的遗传连锁图谱包含2944个SNP标记,总图距3684.3 cM。基于遗传连锁图谱,利用GCIM法共定位到5个QTL,分别分布于1、4、6和10号染色体,单个QTL可解释1.9%~9.9%的表型变异。主效QTL被定位在10号染色体约0.56 Mb的物理区间(127.81~128.37 Mb),关联区间包含有15个有中、高功能变异位点的候选基因,其中TBN-S基因编码磷脂酰乙醇胺结合蛋白(PEBP),属于TFL1类基因,与花序分生和雄穗分枝数发育密切相关。【结论】通过田间表型鉴定、遗传连锁图谱构建和SNP标记定位,将控制玉米雄穗分枝数的主效QTL定位在10号染色体长臂约0.56 Mb物理区间内,包含15个中、高变异度的基因,预测TBN-S为候选基因。 展开更多
关键词 玉米 雄穗分枝数 混池测序 QTL定位 候选基因
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Genetic diversity and differentiation among populations of the pedunculate oak(Quercus robur)at the eastern margin of its range based on a new set of 95 SNP loci
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作者 Bernd Degen Yulai Yanbaev +2 位作者 Ruslan Ianbaev Svetlana Bakhtina Albina Tagirova 《Journal of Forestry Research》 SCIE CAS CSCD 2021年第5期2237-2243,共7页
The aim of this study is to determine the genetic variability and differentiation among populations of pedunculate oak(Quercus robur)at the eastern margin of the species distribution range.We applied new set of 95 geo... The aim of this study is to determine the genetic variability and differentiation among populations of pedunculate oak(Quercus robur)at the eastern margin of the species distribution range.We applied new set of 95 geographically informative nuclear SNP(single nucleotide polymorphism)loci developed using Double Digest Restriction Site Associated DNA(ddRAD),a new generation DNA sequencing technology.The study area is located in the basins of the Volga River and its two main tributaries,Kama and Belaya.Despite the strong reduction and fragmentation of pedunculate oak forests over the last several centuries and comparatively small sizes of present stands,a relatively similar genetic diversity of seven populations was observed(on average,allelic diversity v_(a)=1.472-1.603,observed heterozygosity H_(O)=0.305-0.358,expected heterozygosity H_(E)=0.272-0.343).The genetic distances between populations vary between 0.102 and 0.170(on average,d_(0)=0.128).We have identified the absence of a spatial genetic structure and the existence of genetically peculiar populations in the territory studied.The genetic distances of Gregorius between populations vary between 0.102 and 0.170(on average,d_(0)=0.128).Genetic differentiation among populations was statistically significant with a mean of the fixation index FST of 0.075.Populations from the Privolzhye Upplands and from northern parts of the study area make the most contribution to overall inter-population differentiation(d_(0)=0.137).No significant differences exist among the samples at lowlands along the Volga river(d_(0)=0.110).It was concluded that the multiplicity and different ages of local populations and the complexity of migration routes of the pedunculate oak from Pleistocene refugia and secondary Holocene shelters can cause this phenomenon. 展开更多
关键词 POPULATION Quercus robur gene pool Single nucleotide polymorphism
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Conventional and Molecular Approaches towards Genetic Improvement in Pigeonpea for Insects Resistance 被引量:1
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作者 Arbind K. Choudhary Ranjeet Sharad Raje +2 位作者 Subhojit Datta Rafat Sultana Timmanna Ontagodi 《American Journal of Plant Sciences》 2013年第2期372-385,共14页
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the chara... Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important food legume of the semi-arid tropics (SAT) sustaining livelihood of millions of people. Stagnant and unstable yield per hectare all over the world is the characteristic feature of this crop. This is primarily ascribed to its susceptibility/sensitivity to a number of biotic and abiotic factors. Among biotic factors, insects such as pod borer (Helicoverpa armigera), pod fly (Melanoagromyza obtusa) and spotted borer (Maruca vitrata) substantially damage the crop and result in significant economic losses. Management of these insects by genetic means has always been considered environment friendly approach. However, genetic improvement has always been impeded by limited genetic variability in the primary gene pool of pigeonpea. Wild species present in the secondary and tertiary gene pools have been reported to carry resistance for such insects. However, transfer of resistance through conventional backcrossing has not been much successful. It calls for gene introgression through marker assisted backcrossing (MABC) or advanced backcross breeding (AB breeding). In this review, we have attempted to assess the progress made through conventional and molecular breeding and suggested the ways to move further towards genetic enhancement for insects resistance in 展开更多
关键词 CAJANUS cajan INSECTS RESISTANCE Wild Species Secondary gene pool MABC AB Breeding
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基于基因库求解旅行商问题的遗传算法 被引量:5
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作者 王永 吕致为 《计算机应用研究》 CSCD 北大核心 2023年第11期3262-3268,共7页
针对传统遗传算法(genetic algorithm,GA)求解旅行商问题(traveling salesman problem,TSP)存在寻优效率低、实验结果缺乏一致性等问题,提出了一种基于基因库的遗传算法(genetic algorithm based on genes pool,GPGA)。GPGA从种群中搜... 针对传统遗传算法(genetic algorithm,GA)求解旅行商问题(traveling salesman problem,TSP)存在寻优效率低、实验结果缺乏一致性等问题,提出了一种基于基因库的遗传算法(genetic algorithm based on genes pool,GPGA)。GPGA从种群中搜索减小哈密顿圈长度的边,并当做优良基因构成基因库。父代哈密顿圈在基因库引导下产生更优的子代哈密顿圈,基因库也随着种群的不断进化而同步更新,引导种群个体逐步向最优解靠近。算例结果表明在同样条件下,GPGA比传统遗传算法和几种改进遗传算法的性能更优。 展开更多
关键词 旅行商问题 遗传算法 基因库 局部优化策略
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基于C#的预制构件库与参数化建模的二次开发 被引量:4
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作者 李影 程火焰 +1 位作者 丁浩 屈锋 《土木建筑工程信息技术》 2023年第6期101-105,共5页
随着建筑业向智能化、信息化的转型发展,BIM逐渐成为行业中的重要工具和方法。通过BIM技术进行三维设计,可作为发展装配式建筑信息化的一种方式,现阶段单一的Revit软件还无法满足装配式建筑的所有设计功能。但Revit作为BIM领域最常用的... 随着建筑业向智能化、信息化的转型发展,BIM逐渐成为行业中的重要工具和方法。通过BIM技术进行三维设计,可作为发展装配式建筑信息化的一种方式,现阶段单一的Revit软件还无法满足装配式建筑的所有设计功能。但Revit作为BIM领域最常用的软件之一,拥有丰富的开发接口,可导出多种文件格式,可以通过二次开发实现一个模型运用到整个项目的效果。本文采用C#语言通过Visual Studio平台对Revit进行二次开发,创建装配式预制构件库及参数化建模插件,实现柱、梁、板、剪力墙、楼梯等预制构件的快速绘制和标准化建模。同时在模型建立的基础上,通过插件实现参数化建模,重复性工作被自动化技术所取代,提高了建模的效率。 展开更多
关键词 装配式建筑 BIM 二次开发 族库 参数化建模
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建设中华民族现代文明的实践向度——构建红色基因库传播体系
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作者 常益敏 《青海师范大学学报(社会科学版)》 2023年第3期1-8,共8页
中国共产党在文化建设的成功实践中,形成了以红色文化推动中华优秀传统文化传承创新的规律性认识,红色基因库作为红色文化传承的载体与资源,是当前建设中华民族现代文明的生动教材和“活”的课堂。构建红色基因库传播体系既是理论问题,... 中国共产党在文化建设的成功实践中,形成了以红色文化推动中华优秀传统文化传承创新的规律性认识,红色基因库作为红色文化传承的载体与资源,是当前建设中华民族现代文明的生动教材和“活”的课堂。构建红色基因库传播体系既是理论问题,更是实践问题,在现代文明的观照下具有鲜明的时代性和现实性特征。历史证明,构建红色基因库传播体系的深刻内涵是展现“第二个结合”的文化新形态,增强“四个自信”的思想自觉,建设中华民族文明现代化的必由之路;逻辑结构是把握意识形态要求,掌握传播规律,积淀成功经验;赓续红色文化创新路径是在构建红色基因库传播体系当中,运用创造性转化和创新性发展的方法,推进中华民族现代文明建设。 展开更多
关键词 中华民族现代文明 红色基因库 传播体系 实践理路
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一种基于构建基因库求解TSP问题的遗传算法 被引量:40
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作者 杨辉 康立山 陈毓屏 《计算机学报》 EI CSCD 北大核心 2003年第12期1753-1758,共6页
传统的遗传算法通常被认为是自适应的随机搜索算法 .该文在分析其特点后针对TSP问题提出了一种将建立基因库 (Ge)与遗传算法结合起来的新算法 (Ge-GA) .该算法利用基因库指导种群的进化方向 ,并在此基础上使用全局搜索算子和局部搜索算... 传统的遗传算法通常被认为是自适应的随机搜索算法 .该文在分析其特点后针对TSP问题提出了一种将建立基因库 (Ge)与遗传算法结合起来的新算法 (Ge-GA) .该算法利用基因库指导种群的进化方向 ,并在此基础上使用全局搜索算子和局部搜索算子增强遗传算法的“探测”和“开发”能力 .Ge-GA算法大大加快了遗传算法的收敛速度和寻优能力 .作者测试了TSPLIB中的多个实例 (城市数目从 70~ 15 77) ,试验结果与最优解的误差都不超过0 .0 0 1% .特别是对于难求解的TSP问题 ,如att5 3 2和fl15 77,都能够在理想的时间内找到最优解 . 展开更多
关键词 旅行商问题 基因库 TSP问题 遗传算法 随机搜索算法 组合优化问题
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豌豆属(Pisum)SSR标记遗传多样性结构鉴别与分析 被引量:18
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作者 宗绪晓 Rebecca Ford +2 位作者 Robert R Redden 关建平 王述民 《中国农业科学》 CAS CSCD 北大核心 2009年第1期36-46,共11页
【目的】评价豌豆属(Pisum L.)2个种5个亚种下,共8个资源类群的遗传多样性水平,揭示豌豆属下资源群体结构及其遗传关系远近,验证传统植物学分类的可靠程度,为充分发掘、利用豌豆野生种质提供必要信息。【方法】利用21对豌豆多态性SSR引... 【目的】评价豌豆属(Pisum L.)2个种5个亚种下,共8个资源类群的遗传多样性水平,揭示豌豆属下资源群体结构及其遗传关系远近,验证传统植物学分类的可靠程度,为充分发掘、利用豌豆野生种质提供必要信息。【方法】利用21对豌豆多态性SSR引物,对来自世界5大洲62个国家的豌豆属94份栽培种质(P.sativum ssp.sativum var.sativum)及其1个近缘野生种(P.fulvum),3个野生亚种(P.sativum ssp.abyssinicum、P.sativum ssp.asiaticum、P.sativum ssp.transcaucasicum)和3个野生变种(P.sativum ssp.elatius var.elatius、P.sativum ssp.elatius var.pumilio、P.sativum ssp.sativumvar.arvense)的103份野生种质进行SSR标记遗传多样性分析;利用NTSYSpc2.2d软件估算其遗传距离,进行主成分分析(PCA)并绘制三维空间聚类图;利用Popgene V1.32估算种质群间的Nei78遗传距离等参数并进行UPGMA聚类分析,采用MEGA3.1绘制种质群间聚类图;采用Popgene V1.32估算种质群的等位位点分布等参数,利用Fstat V2.9.3.2进行种质群间遗传多样性差异显著性测验。【结果】21对豌豆多态性SSR引物共扩增出104条多态性带,每对引物平均扩增出4.95个等位变异,其中有效等位变异占65.56%;PSAD270,PSAC58,PSAA18,PSAC75,PSAA175和PSAB72等SSR引物最为有效。SSR等位变异在豌豆属植物学分类单位中分布均匀,但分类单位种质群间的遗传多样性在多数情况下差异显著。豌豆属野生种P.fulvum的遗传多样性远低于栽培种P.sativum;豌豆栽培种下,P.sativum ssp.sativum var.sativum和P.sativum ssp.asiaticum的遗传多样性最高,P.sativum ssp.elatius var.elatius和P.sativum ssp.transcaucasicum次之,P.sativum ssp.elatius var.pumilio、P.sativum ssp.sativum var.arvense和P.sativumssp.abyssinicum最低。PCA分析发现,豌豆属种质资源由4个差异明显的基因库构成。"fulvum"基因库主要由野生种Pisum fulvum资源构成,"abyssinicum"基因库主要由栽培种下的P.sativum ssp.abyssinicum亚种资源构成,"arvense"基因库主要由栽培种下的P.sativum ssp.sativumvar.arvense变种资源构成;"sativum"基因库由P.sativum ssp.asiaticum、P.sativum ssp.elatius var.elatius、P.sativum ssp.transcaucasicum、P.sativum ssp.elatius var.pumilio和P.sativum ssp.sativum var.sativum资源构成。"sativum"基因库构成豌豆栽培资源初级基因库;"fulvum"、"abyssinicum"和"arvense"基因库共同构成豌豆栽培资源次级基因库。植物学分类单位间的Nei78遗传距离介于7.531~35.956,UPGMA聚类方法将豌豆属植物学分类单位聚成3个组群,"组群I"对应"sativum"和"arvense"基因库之和,"组群II"对应"abyssinicum"基因库,"组群III"对应"fulvum"基因库,聚类结果支持4个基因库的划分。【结论】豌豆属下多数植物学分类单位间遗传多样性差异显著,并分化成4个基因库。研究结果部分支持豌豆属下传统的植物学分类体系,并指出了其合理与不足之处。为拓宽豌豆育成品种的遗传基础,应充分发掘豌豆属下各基因库的遗传潜力。 展开更多
关键词 豌豆属(Pisum L.) SSR 遗传多样性 植物学分类体系 基因库
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美洲黑杨基因资源收存及其遗传评价的研究 被引量:30
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作者 张绮纹 苏晓华 +2 位作者 李金花 陈一山 解荷峰 《林业科学》 EI CAS CSCD 北大核心 1999年第2期31-37,共7页
从17个国家引进331个黑杨派无性系(其中52个美洲黑杨无性系),在山东省长清县营建我国第一个黑杨派无性系基因库。连续10年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和... 从17个国家引进331个黑杨派无性系(其中52个美洲黑杨无性系),在山东省长清县营建我国第一个黑杨派无性系基因库。连续10年对基因库内美洲黑杨无性系进行了多性状系统研究,结果表明各无性系间在物候期、生长、生根、抗寒和抗病虫等方面均存在着显著的差异,遗传变异丰富;系间的主要性状与无性系起源纬度及各性状间存在一定相关性。主要材料性状变异的研究结果表明,38个8年生美洲黑杨无性系在木材基本密度、纤维长度无性系间变异达到极显著水平;木材密度和纤维长度与树高和胸径呈一定相关性;木材密度和纤维长度这两个性状在遗传上相互独立,受不同遗传机制控制。利用分子遗传标记RAPD技术研究库内美洲黑杨无性系DNA多态性,结果表明本库美洲黑杨DNA多态率为86%,再次证明本库美洲黑杨遗传多样性高,可为我国杨树改良提供丰富的育种材料。 展开更多
关键词 美洲黑杨 基因库 主要性状 遗传分析
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东方田鼠骨髓基因池的构建及抗日本血吸虫抗性相关基因的筛选 被引量:10
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作者 秦志强 胡维新 +4 位作者 邬国军 许冰 申群喜 龚强 吴驰 《生命科学研究》 CAS CSCD 2004年第4期333-338,共6页
利用表达克隆法,从东方田鼠骨髓细胞中克隆出抗日本血吸虫抗性相关基因.首先提取高质量的mRNA,逆转录成cDNA,将cDNA与哺乳动物细胞瞬时表达载体pcDNA1.1/Amp连接,建立表达cDNA文库;将cDNA文库分成A-H8个基因池,转染HEK293细胞,48h后收... 利用表达克隆法,从东方田鼠骨髓细胞中克隆出抗日本血吸虫抗性相关基因.首先提取高质量的mRNA,逆转录成cDNA,将cDNA与哺乳动物细胞瞬时表达载体pcDNA1.1/Amp连接,建立表达cDNA文库;将cDNA文库分成A-H8个基因池,转染HEK293细胞,48h后收集培养上清,获得条件培养基.将条件培养基与日本血吸虫童虫一起培养,观察杀虫效应,取对童虫抑制作用最强的基因池E再分成8个亚基因池E1-8,分别转染HEK293细胞,并将条件培养基与血吸虫童虫一起培养,获得具有明显抑制血吸虫童虫活性的亚基因池E77,按上述方法反复进行筛选,直到获得有抑制作用的单个克隆,该技术的建立为克隆东方田鼠抗日本血吸虫抗性相关基因以及研究其作用机制奠定基础. 展开更多
关键词 东方田鼠 基因池 表达克隆法 日本血吸虫 抗性相关基因
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云山国家森林公园土壤动物资源生态特征 被引量:15
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作者 唐本安 唐果 +1 位作者 唐敏 李翠娥 《自然资源学报》 CSSCI CSCD 北大核心 2003年第4期499-504,共6页
1998年5月至2001年5月对云山国家森林公园土壤动物资源进行了生态特征研究,共获得土壤动物9769个,隶属6门13纲31个类目。亚热带土壤动物的类群在这里几乎都有分布。其中优势类群为蜱螨目、弹尾目及线虫类3类;常见类群有膜翅类、双尾目... 1998年5月至2001年5月对云山国家森林公园土壤动物资源进行了生态特征研究,共获得土壤动物9769个,隶属6门13纲31个类目。亚热带土壤动物的类群在这里几乎都有分布。其中优势类群为蜱螨目、弹尾目及线虫类3类;常见类群有膜翅类、双尾目、原尾目、线蚓类、双翅目5类;其余为稀有类群。通过与我国东西方向及其南北方向上典型土壤动物生态群落的分析比较,研究过程发现,受其地表生物多样性的影响,云山土壤动物资源种群丰富;原始群落结构完整;区系组成地带过渡性明显;山体顶部灌丛地段节肢动物有个体偏大的生态特征。研究结果表明:地处我国南岭、雪峰山及滇黔桂地区3种不同类型山系过渡地带的云山国家森林公园,作为自然保护区,不但是一个资源丰富的地表生物生态基因库,同时还是一个异常宝贵的地下生物生态基因库。 展开更多
关键词 云山 土壤动物资源 生态特征 生态基因库
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东北地区大豆品种血缘组成分析 被引量:29
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作者 孙志强 田佩占 王继安 《大豆科学》 CAS CSCD 北大核心 1990年第2期112-120,共9页
用系谱分析法研究了东北地区168个杂交育成大豆品种与其祖先品种间的亲缘系数,估计了各祖先品种对辽,吉、黑三省大豆基因库的相对遗传贡献。结果表明,我国东北地区大豆育成品种的遗传基础较窄。满仓金,紫花4号,丰地黄,元宝金,荆山朴,铁... 用系谱分析法研究了东北地区168个杂交育成大豆品种与其祖先品种间的亲缘系数,估计了各祖先品种对辽,吉、黑三省大豆基因库的相对遗传贡献。结果表明,我国东北地区大豆育成品种的遗传基础较窄。满仓金,紫花4号,丰地黄,元宝金,荆山朴,铁荚四粒黄,克山四粒黄,金元1号,十胜长叶和黄宝珠10个祖先品种对东北大豆杂交育成品种遗传基础的总贡献为57.7%。因满仓金,元宝金是金元×黄宝珠的后代,而荆山朴是由满仓金系选而来,因此,金元和黄宝珠约贡献了东北大豆育成品种约28.7%的遗传物质。三省比较,黑龙江省杂交育成大豆品种的遗传基础最窄,辽宁次之。为了保证持续稳定的育种进展,有必要加强对国内外大豆种质资源利用的研究。扩大种质资源的利用范围,使大豆品种的遗传基础多样化。 展开更多
关键词 大豆 品种 血缘组成 东北
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山茶属植物种质资源的搜集及基因库的建立利用研究 被引量:13
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作者 庄瑞林 董汝湘 +8 位作者 黄爱珠 邱金兴 匡元玉 曾庆南 王德斌 陈永忠 蔡肖群 邓晓安 苏明媚 《林业科学研究》 CSCD 北大核心 1991年第2期178-184,共7页
采用普通油茶大砧嫁接快速繁殖建成4个基因库,现已搜集保存种质资源2 267个号,其中山茶物种、变种161个,山茶花品种340多个,油茶农家品种、优株,无性系等1 605个。将不同纬度、海拔高度和生态条件下的物种、品种集中在同一地进行研究,... 采用普通油茶大砧嫁接快速繁殖建成4个基因库,现已搜集保存种质资源2 267个号,其中山茶物种、变种161个,山茶花品种340多个,油茶农家品种、优株,无性系等1 605个。将不同纬度、海拔高度和生态条件下的物种、品种集中在同一地进行研究,提高了种质对环境的适应性和抗性,发挥了基因库的多种作用和效益。 展开更多
关键词 山茶属 种质资源 基因库 良种选育
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国家水禽基因库七个家鸭群体遗传多样性检测 被引量:10
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作者 吉文林 钱凯 +4 位作者 李慧芳 葛洪伟 赵旭庭 段修军 陈宽维 《中国家禽》 北大核心 2006年第24期72-74,共3页
试验通过微卫星标记对国家水禽种质资源基因库保存的7个家鸭保种群体的保种效果进行了遗传检测。结果表明:28个微卫星位点中有25个高度多态位点,可作为有效遗传标记用于各群体的遗传检测。7个鸭群体中,平均杂和度范围是0.5137~0.6035,... 试验通过微卫星标记对国家水禽种质资源基因库保存的7个家鸭保种群体的保种效果进行了遗传检测。结果表明:28个微卫星位点中有25个高度多态位点,可作为有效遗传标记用于各群体的遗传检测。7个鸭群体中,平均杂和度范围是0.5137~0.6035,最高的是荆江麻鸭,最低的是山麻鸭,反映了各鸭群体保持了较丰富的遗传多样性。Ds遗传距离表明各品种间的遗传距离较远,巢湖鸭与金定鸭之间最远,为0.8835;金定鸭与山麻鸭最近,为0.2674。7个家鸭群体间的Ds值都比较大,说明各群体间的基因交流较少。上述结果反映了在国家水禽种质资源基因库所保存的7个地方鸭群体的保种方法良好,保种效果有效。 展开更多
关键词 微卫星 基因库 保种效果
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