An α fetoprotein (AFP) antibody gene probe was constructed using chlorophyll molecule as a coupler between protein and dsDNA. The preliminary study on the detection of AFP using this novel probe was performed by immu...An α fetoprotein (AFP) antibody gene probe was constructed using chlorophyll molecule as a coupler between protein and dsDNA. The preliminary study on the detection of AFP using this novel probe was performed by immuno PCR, and the results indicated that the sensitivity of the gene probe by immuno PCR is 10 4-10 5 times higher compared with ELISA. The construction of immuno PCR gene probe in this way not only completely prevents the protein from contacting with organic solvent and maintains the native conformation of the proteins, but also anchors protein to dsDNA in a fixed orientation and makes PCR amplification more efficient. The gene probe thus constructed is stable for at least 6 months at room temperature. This new approach is exquisite, simple, less expensive, and suitable to a variety of applications.展开更多
Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific...Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific genetic diversity in tick populations. In this study, the genetic diversity of four H. dromedarii populations was investigated using mitochondrial cytochrome c oxidase subunit I (COI) gene and randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). The results showed that both of the aforementioned techniques produced similar grouping patterns. Moreover, they revealed that the four tick populations had high levels of genetic similarity. However, one population was slightly different from the three other populations. The current study demonstrated that H. dromedarii ticks in the UAE are very similar at the genetic level and that investigating more locations and screening larger numbers of ticks could reveal larger genetic differences.展开更多
To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strai...To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.展开更多
文摘An α fetoprotein (AFP) antibody gene probe was constructed using chlorophyll molecule as a coupler between protein and dsDNA. The preliminary study on the detection of AFP using this novel probe was performed by immuno PCR, and the results indicated that the sensitivity of the gene probe by immuno PCR is 10 4-10 5 times higher compared with ELISA. The construction of immuno PCR gene probe in this way not only completely prevents the protein from contacting with organic solvent and maintains the native conformation of the proteins, but also anchors protein to dsDNA in a fixed orientation and makes PCR amplification more efficient. The gene probe thus constructed is stable for at least 6 months at room temperature. This new approach is exquisite, simple, less expensive, and suitable to a variety of applications.
文摘Hyalomma dromedarii ticks are important disease vectors to camels in the UAE and worldwide. Ticks can be identified using DNA-based techniques. In addition, such techniques could be utilized to study the intraspecific genetic diversity in tick populations. In this study, the genetic diversity of four H. dromedarii populations was investigated using mitochondrial cytochrome c oxidase subunit I (COI) gene and randomly amplified polymorphic DNA polymerase chain reaction (RAPD-PCR). The results showed that both of the aforementioned techniques produced similar grouping patterns. Moreover, they revealed that the four tick populations had high levels of genetic similarity. However, one population was slightly different from the three other populations. The current study demonstrated that H. dromedarii ticks in the UAE are very similar at the genetic level and that investigating more locations and screening larger numbers of ticks could reveal larger genetic differences.
文摘To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.
