Expression of the novel gene NM23-H1B in ovarian cancer

Objective:To study the expression of the human novel gene NM23-H1B in ovarian cancer. Methods: Totally 24 samples from patients with epithelial ovarian tumor at different clinical stages and 4 from normal ovaries were examined for NM23-H1B mRNA expression by RT-PCR and Northern blot. Results: All samples expressed NM23-H1B mRNA through RT-PCR, while the level of expression in ovarian tumor was higher than that of normal ovary. The results of Northern blot showed that NM23-H1B was overexpressed in ovarian cancer while lowexpressed in normal ovary or low malignant potential (LMP). The level of expression at early stage cancer(stageⅠand Ⅱ) was higher than those in advanced cancer(stage Ⅲ and Ⅳ). In early stage carcinoma, the expression level was involved in the differentiation of tumor cell, and well-differentiated cancer expressed NM23-H1B mRNA in comparatively higher level. Conclusion: The novel gene NM23-N1B is closely correlated with the ovarian cancer.

EXPRESSION OF NM23-H1 GENE PRODUCT IN NASOPHARYNGEAL CARCINOMA AND ITS CLINICAL SIGNIFICANCE

Objective: The nm23 gene is one of the tumor metastatic suppressor genes. The expression of nm23H1 has been reported to be inversely associated with metastatic potentiality in a number of human carcinomas, including breast, colorectal, gastric, hepatocellular and gallbladder carcinomas. In this study, the immunohistochemical staining of nm23H1 protein in human nasopharyngeal carcinoma (NPC) was examined, and the relationship between nm23H1 and both metastasis and prognosis of patients with NPC was also investigated. Methods: Routine LSAB immunohistochemistry with the nm23H1 monoclonal murine antibody was employed to study the expression of nm23H1 protein in 95 paraffinembedded specimens of NPC treated at our hospital. The clinical pathologic data and results of followup were also retrieved. Comparisons between patients with and without expression of nm23H1 protein with respect to metastasis, locoregional recurrence and survival were performed using Log rank test. Multivariate prognostic analyses were performed by using Cox's regression model. Results: Nm23H1 negative expressive tumors were associated with a higher incidence of lymphnode metastasis (86.7%) than those of nm23H1 positive (48.6%, P<0.01). Nm23H1 negative expressive tumors were associated with a high incidence of recurrence and distant metastasis after radiotherapy (P<0.05). A significant association was found between expression of nm23H1 and prognosis (P<0.01). The expression of nm23H1 indicated favorable prognosis. Conclusion: It was suggested that nm23 H1 negative expression was significantly associated with lymphnode metastasis, recurrence and distant metastasis. Nm23H1 may have value for predicting the prognosis of NPC.

The Regulating Effect of the nm23-H1 Gene for PKA Signal Pathway of Lung Cancer Cell and Its Molecular Mechanism

Backgroud and Objective Lung cancer has become the most frenquent malignant tumor in the world which its mortality and morbidity is increasing fastest among all the cancers。

Alterations and Its Mechanisms of Wnt Signal Pathway in Human High-matastatatic Large Cell Lung Cancer Cell Line L9981 by Transfecting with Nm23-H1 Gene

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the main cause of failure to cure and lose their life of the

Influence of Nm23-H1 Gene Site Mutagenesis on Invasive And Metastatic Phenotype in Human High-Metastatic Large Cell Lung Cancer Cell Line L9981

Background and Objective Invasion and metastasis is not only the malignant phenotypes of lung cancer but also the main cause of death. To study and elucidate the molecular mechanism

Transfection of Nm23-H1 Gene Inhibited the Metastatic Potential of Human High-metastatic Large Cell Lung Cancer L9981

Background and objective Invasion and metastasis is not only the malignant phenotypes of lung cancer but also the main cause of death. Elucidating the molecular mechanism of

The Molecular Mechanisms of Nm23-H1 Gene Transfection on Reversing Invasion and Metastasis Phenotype in Human High-metastataic Large Cell Lung Cancer Cell Line L9981

Background and Objective Our previous studies have proved that nm23-H1 gene was a tumor metastatic suppressive gene, tumor metastasis phenotype of human lung cancer could

Alterations in the Cytoskeleton and Biological Behavior in Human Lung Cancer Cell Line L9981 by Nm23-H1 gene Transfection

Objective and Methods The key cause of failure to cure and high mortality in lung cancer. At present, it has been known

Alteration in Gene Expression Prof ile and Biological Behavior in Human Lung Cancer Cell Line NL9980 by Nm23-H1 Gene Silencing

Background and Objective Lung cancer is the leading cause of cancer death in both men and women. Tumor metastasis is an essential aspect of lung cancer progression. Nm23-H1 is

The Regulating Mechanism of Nm23-H1 Gene for FAK Signal Pathway in Human Lung Cancer Cell Line L9981

Background and objective Lung Cancer is one of the most malignant cancers threatening people’s health and life and one of the most rapid increasing cancers both in morbidity

Changes of the Proteomic Profiles in Human High-metastatic Large Cell Lung Cancer Cell Line L9981 by Transfecting with nm23-H1 Gene

Background and Objective Cancer metastasis is not only the malignant characteristics of lung cancer, but also the key cause of failure to cure and high mortality. It has been proved

Comparative Proteomics Study on Human High-metastatic Large Cell Lung Cancer Cell Lines Before and After Transfecting with Nm23-H1 Gene

Background and Objective Lung cancer is not only the most dangerous threating tumor to human’s health and life, but also a malignant tumor with poor prognosis. In the past 10 years,

Survivin nm23-H1在非小细胞肺癌中的表达及相关性研究

目的:研究肿瘤相关基因survivin、nm23-H1在非小细胞肺癌、NSCLC、组织中的表达及相关性。探讨其与临床病理特征之间的关系。方法:采用免疫组化SP法检测survivin和nm23-H1在52例NSCLC组织、20例对应癌旁组织和15例正常组织中的表达情况。结果:survivin的表达癌组织明显高于癌旁组织的表达,差异具有显著性(P<0.05),正常组织无survivin表达;nm23-H1的表达癌组织明显低于癌旁组织的表达,差异具有显著性(P<0.05);Survivin在NSCLC组织中的表达与年龄、性别、组织类型、淋巴结转移无关(P>0.05),nm23-H1在伴有淋巴结转移的NSCLC组织中表达的阳性率为19.0%(4/21),在未伴有淋巴结转移的NSCLC组织中表达的阳性率为48.4%(15/31),两者差异有显著性;survivin在TNM(Ⅰ+Ⅱ)期NSCLC组织中表达低于在TNM(Ⅲ+Ⅳ)期NSCLC组织中表达;nm23-H1在TNM(Ⅰ+Ⅱ)期NSCLC组织中表达高于在TNM(Ⅲ+Ⅳ)期NSCLC组织中表达,差别有显著意义(P<0.05);survivin在高分化NSCLC中表达低于在低分化NSCLC中表达;nm23-H在高分化NSCLC组织中表达高于在低分化NSCLC组织中表达,差别有显著意义(P<0.05);survivin、nm23-H1在NSCLC中表达负相关(P<0.05)。结论:survivin和nm23-H1的表达与NSCLC发生、临床进展及预后密切相关。Survivin可以作为NSCLC的早期诊断指标,并可以根据sur-vivin与nm23-H1的联合检测来判断恶性程度及预后。

Changes of histology and expression of MMP-2 and nm23-H1 in primary and metastatic gastric cancer

AIM: To investigate the changes of histology and expression of MMP-2 and nm23-H1 in primary and metastatic gastric cancer. METHODS: One hundred and seventy-seven gastric cancer patients with lymph node and/or distal metastasis between 1997 and 2001 were reviewed. Differences in histology of the primary and metastatic gastric cancer were assessed. MMP-2 and nm23-H1 immunoreactivity was compared in 44 patients with tumor infiltration to the serosa layer. RESULTS: Poorly and moderately differentiated metastatic gastric cancer was found in 88.7% (157/177) and primary gastric cancer in 75.7% (134/177) of the patients. The histological type of metastatic gastric cancer that was not completely in accordance with the preponderant histology of primary gastric cancer was observed in 25 patients (14.1%). MMP-2 immunoreactivity in metastatic gastric cancer was significantly stronger than that in primary gastric cancer, while nm23-H1 immunoreactivity showed no difference in primary and metastatic gastric cancer. CONCLUSION: Metastatic gastric cancer presents more aggressive histological morphology and higher MMP-2 immunoreactivity than primary gastric cancer. This heterogeneity may elicit a possible mechanism of gastric cancer metastasis.

电离辐射对人肺非小细胞癌细胞株H446 nm23-H1基因表达的影响

目的：探讨电离辐射对人肺非小细胞癌细胞株H446 nm23-H1基因表达的影响。方法：取对数生长期的H446细胞置^60Co放射治疗机上单次照射，照射剂量分别为0、1、2．3、4和5Gy，继续培养至0、6、24和48h后收集细胞，提取细胞总RNA和总蛋白。分别用RT-PCR和Western Not检测nm23-H1基因mRNA和蛋白的表达水平。结果：照射后各剂量组nm23-H1 mRNA和蛋白的表达量均明显降低（P〈0．01），与剂量之间呈负相关（P〈0．05），相关系数r分别≥0．785和≥0．66；mRNA的表达水平在0～24h内随培养时间延长逐渐增加，48h时有所降低。蛋白表达在0～6h内没有明显变化，24h达到高点，48h有所降低。结论：电离辐射对H446细胞nm23-H1基因表达有一定的影响，随着辐射剂量的增大该基因mRNA和蛋白表达水平逐渐降低，并且在所有观察时间点均显示出一定的剂量效应关系。

肝细胞癌中微血管密度和转化生长因子-β_1 nm23-H1蛋白表达及意义

目的 探讨肝细胞癌(Hepatocelluar carcinoma,HCC)和癌旁肝组织强转化生长因子β1(Transforming growth factor-β1,TGF-β1),nm23-H1蛋白的表达及微血管密度(Microvessel tensity,MVD)的变化。方法 采用免疫组化方法对45例HCC和癌旁肝组织进行TGF-β1多克隆抗体、nm23-H1单克隆抗体和第Ⅷ因子相关抗原单克隆抗体染色。结果 TGF-β1及nm23-H1蛋白表达在HCC和癌旁肝组织两者之间差异有显著性意义(P<0.05);HCC组织中TGF-β1表达强度与肿瘤间质MVD之间存在正相关(P<0.05);三者与HCC有无转移呈显著性相关(P<0.05),但与肿瘤大小无关(P>0.05)。结论 TGF-β1及nm23-H1蛋白表达强度和肿瘤MVD的变化与HCC转移有关,上述3项指标对评估HCC的转移及预后有重要意义。

IDENTIFICATION OF A NEW HUMAN nm23 GENE,nm23-H3b

Nm23 is a kind of effective tumor metastasis suppressor genes which included two types in human:nm23-H1 and nm23-H2. Amino acid identity between nm23-H1 and nm23-H2 was 88%.In this study,using a pair of primers to flank the part of coding sequence of nm 23,the 5'-translated sequence was amplified by polymerase chain reaction (PCR) from human normal liver genomic DNA.A 375-base pairs clone was charactertzed,which designated pnm23-H3b.The nm23-H3b nucleotide sequence between 40 bp and 70 bp is different from nm23-H1 and nm23-H2,and other sequences have 86%and 90%identical to nm23-H1 and nm23-H2,respectively.Southern blot containing BglⅡ-digested human liver genomic DNA hybridized to the entire nm23-H3b DNA and showed three bands at 10.5,7.9 and 4.0 Kb.These data demonstrate that the third human nm23 exists possibly.Therefore,nm23 may be considered a family of closely related genes.

Down-regulation and It's Molecular Biology of Nm-23H1 Gene Transfection on PKC Signal Path-way in Human High-metastatic Large Cell Lung Cancer Cell Line L9981

Backgroud and Objective At present, lung cancer has become the most frenquent malignant tumor in China and in the world which its mortality and morbidity is increasing fastest.

Down-regulation and It's Molecular Biology of Nm-23H1 gene Transfection on Ras-to-MAPK Signal Transduction Pathway in Human High-metastatic Large Cell Lung Cancer Cell Line L9981

Backgroud and Objective Tumor metastasis is not only the malignant marker and characteristics of lung cancer, but also the key cause of failure to cure and lose their life of the patients

Molecular mechanism for effects of NM23-H1 on primary hepatocellular carcinoma with intrahepatic metastasis

To further investigate the molecular mechanism for primary hepatocellular carcinoma (HCC) with intrahepatic metastasis. Methods: The expression of NM23-H1 in 30 patients with HCC was determined. Meanwhile, the full-length cDNA of NM23-H1 was trans feeted into a highly metastatic HCC cell line to observe the effects of NM23-H1 on HCC cells in vitro. Results: The patients with intrahepatic metastasis, formation of portal vein tumor thrombus and poor cell differentiation showed a marked reduction of NM23-H1 mRNA. In vitro, the potential of migration and invasion of the NM23-H1-transfected cells was remarkably inhibited. Conclusion: Low expression of NM23-H1 mRNA contributes to the enhancement of invasive and metastatic potential in HCC and to promote its expression might be beneficial for preventing inahepatic metastasis of primary HCC.