Objective： To study the effects of Bushen Yisui Capsule（补肾益髓胶囊, BSYSC） on the oligodendrocyte lineage genes（Olig） 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis（EAE） in order t...Objective： To study the effects of Bushen Yisui Capsule（补肾益髓胶囊, BSYSC） on the oligodendrocyte lineage genes（Olig） 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis（EAE） in order to explore the remyelination effect of BSYSC. Methods： The mice were randomly divided into normal control（NC）, EAE model（EAE-M）, prednisone acetate（PA, 6 mg/kg）, BSYSC high-dose（3.02 g/kg） and BSYSC low-dose（1.51 g/kg） groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein（MOG） 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin（HE） staining and transmission electron microscope（TEM）. The protein expressions of myelin basic protein（MBP）, Olig1 and Olig2 in brains were measured by immunohistochemistry. The m RNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Results： Compared with the EAE-M mice,（1） the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40（P〈0.01）;（2） the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice;（3） the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively（P〈0.05 or P〈0.01）;（4） the protein expression of Olig1 was increased in BSYSC（3.02 g/kg）-treated EAE mice on day 40（P〈0.01）. Protein and m RNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40（P〈0.01）. Conclusion： The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.展开更多
目的:探讨少突胶质细胞谱系基因-髓鞘碱性蛋白(genes of the oligodendrocyte lineage-myelin basic protein,Golli-MBP)在口腔扁平苔藓(oral lichen planus,OLP)组织中的表达及其与OLP发病的关系。方法:采用实时荧光定量PCR的方法,检...目的:探讨少突胶质细胞谱系基因-髓鞘碱性蛋白(genes of the oligodendrocyte lineage-myelin basic protein,Golli-MBP)在口腔扁平苔藓(oral lichen planus,OLP)组织中的表达及其与OLP发病的关系。方法:采用实时荧光定量PCR的方法,检测39例OLP患者病损组织和16例健康口腔黏膜组织中Golli-MBP的基因表达水平,采用免疫组化法检测38例OLP及20例正常口腔黏膜组织石蜡标本中Golli-MBP的表达情况。结果:Golli-MBP mRNA在OLP组中的表达显著高于正常对照组(P<0.001)。OLP患者石蜡标本中Golli-MBP的表达水平亦显著高于对照组(P<0.001);固有层淋巴细胞中Golli-MBP的表达高于对照组(P<0.05);上皮细胞中Golli-MBP的表达在OLP患者及对照组间无统计学差异(P>0.05)。结论:Golli-MBP在OLP组织中高表达,可能在OLP的发病机制中起一定作用。展开更多
基金Supported by the National Natural Science Foundation(Nos.81072765,81173237 and 81273742)Beijing Natural Science Foundation(No.7142053)+2 种基金Scientific Research Key Program of Beijing Municipal Commission of Education(No.KZ201310025023)Program for Changcheng Scholars of the ImportationDevelopment of High-Caliber Talents Project of Beijing Municipal Institutions(No.CIT&TCD20140329)
文摘Objective： To study the effects of Bushen Yisui Capsule（补肾益髓胶囊, BSYSC） on the oligodendrocyte lineage genes（Olig） 1 and Olig2 in C57BL/6 mice with experimental autoimmune encephalomyelitis（EAE） in order to explore the remyelination effect of BSYSC. Methods： The mice were randomly divided into normal control（NC）, EAE model（EAE-M）, prednisone acetate（PA, 6 mg/kg）, BSYSC high-dose（3.02 g/kg） and BSYSC low-dose（1.51 g/kg） groups. The mice were induced by immunization with myelin oligodendrocyte glycoprotein（MOG） 35-55. The neurological function scores were assessed once daily. The pathological changes in mice brains were observed with hematoxylin-eosin（HE） staining and transmission electron microscope（TEM）. The protein expressions of myelin basic protein（MBP）, Olig1 and Olig2 in brains were measured by immunohistochemistry. The m RNA expressions of Olig1 and Olig 2 was also determined by quantitative real-time polymerase chain reaction. Results： Compared with the EAE-M mice,（1） the neurological function scores were significantly decreased in BSYSC-treated mice on days 22 to 40（P〈0.01）;（2） the inflammatory cells and demyelination in brains were reduced in BSYSC-treated EAE mice;（3） the protein expression of MBP was markedly increased in BSYSC-treated groups on day 18 and 40 respectively（P〈0.05 or P〈0.01）;（4） the protein expression of Olig1 was increased in BSYSC（3.02 g/kg）-treated EAE mice on day 40（P〈0.01）. Protein and m RNA expression of Olig2 was increased in BSYSC-treated EAE mice on day 18 and 40（P〈0.01）. Conclusion： The effects of BSYSC on reducing demyelination and promoting remyelination might be associated with the increase of Olig1 and Olig2.
文摘目的:探讨少突胶质细胞谱系基因-髓鞘碱性蛋白(genes of the oligodendrocyte lineage-myelin basic protein,Golli-MBP)在口腔扁平苔藓(oral lichen planus,OLP)组织中的表达及其与OLP发病的关系。方法:采用实时荧光定量PCR的方法,检测39例OLP患者病损组织和16例健康口腔黏膜组织中Golli-MBP的基因表达水平,采用免疫组化法检测38例OLP及20例正常口腔黏膜组织石蜡标本中Golli-MBP的表达情况。结果:Golli-MBP mRNA在OLP组中的表达显著高于正常对照组(P<0.001)。OLP患者石蜡标本中Golli-MBP的表达水平亦显著高于对照组(P<0.001);固有层淋巴细胞中Golli-MBP的表达高于对照组(P<0.05);上皮细胞中Golli-MBP的表达在OLP患者及对照组间无统计学差异(P>0.05)。结论:Golli-MBP在OLP组织中高表达,可能在OLP的发病机制中起一定作用。
