[Objective] The paper was to improve the blast resistance of insect-resistant transgenic rice. [Method] The Japonica rice variety Jikang10, a new transgenic variety with exogenous insect-resistant gene Cry1C, was used...[Objective] The paper was to improve the blast resistance of insect-resistant transgenic rice. [Method] The Japonica rice variety Jikang10, a new transgenic variety with exogenous insect-resistant gene Cry1C, was used as the receptor, and Kongyu 131, a traditional breeding variety with broad-spectrum high blast resistance genes Pi1 and Pi2, was used as the donor to breeding new rice varieties. The genes were polymerized by hybridization and multi-generation backcrossing, and the offspring of each generation was screened by molecular marker assisted selection, field identification of multi-resistance against insect pests and diseases and agronomic trait selection. [Result] Four lines SK01, SK02, SK03 and SK04 with better resistances to insect pests and blast and outstanding agronomic traits in field were selected. [Conclusion] The results will lay foundations for breeding new multi-resistance rice varieties in Huanghuai rice region.展开更多
Summary： The ATP2C1 gene mutation in one ease of familial benign chronic pemphigus was investigated.One patient was diagnosed as familial benign chronic pemphigus by pathology, ultrastructral examination and clinical...Summary： The ATP2C1 gene mutation in one ease of familial benign chronic pemphigus was investigated.One patient was diagnosed as familial benign chronic pemphigus by pathology, ultrastructral examination and clinical features. Genomic DNA was extracted from blood samples. Mutation of ATP2CI gene was detected by polymerase chain reaction （PCR） and DNA sequencing. The results showed that deletion mutation was detected in ATP2C1 gene in this patient, which was 2374delTTTG. No mutation was found in the family members and normal individuals. It was coneluded that the 2374delTTTG mutation in ATP2C1 gene was the specific mutation for the clinical phenotype for this patient and was a de novo mutation.展开更多
缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HI...缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HIF-1α敲除的RNA-seq数据,HIF-1α的ChIP-Seq数据,HIF-1α在肝癌中的共表达基因,以及肝癌相关的GEO(Gene Expression Omnibus)数据集,寻找HIF-1α的潜在靶基因。通过分析TCGA(The Cancer Genome Atlas)肝癌数据库、GEO和HPA(Human Protein Atlas)数据集,研究HIF-1α与ATP2C1的相关性,ATP2C1在肝癌中的表达及预后。通过建立物理和化学(氯化钴)缺氧模型验证ATP2C1与低氧及HIF-1α的关系。通过GO(Gene Ontology),KEGG(Kyoto Encyclopedia of Genes and Genomes)和GSEA(Gene Set Enrichment Analysis)分析探索ATP2C1的生物学功能。通过设计体外实验证实ATP2C1对HCC的作用。利用STRING和BioGRID两个蛋白互作在线数据库获得ATP2C1的互作蛋白,并研究其在肝癌中的表达及相关性。通过整合及筛选数据,ATP2C1被鉴定为一个HIF-1α的潜在靶基因。ATP2C1与HIF-1α高度相关,在肝细胞癌中高表达,且伴随有不良预后。富集分析与体外实验的结果表明ATP2C1参与调控HCC细胞的增殖迁移。蛋白互作数据表明ATP2C1与TMEM165存在互作关系,生存分析表明TMEM1651高表达的肝癌患者预后较差。相关性分析的结果显示ATP2C1与肝癌中TMEM165和MMP2的表达高度相关,表明ATP2C1可能与TMEM165和MMP2存在互作关系,并参与了肝癌的进展过程。结果表明,ATP2C1是HIF-1α的靶基因和肝细胞癌的生物标志物,其敲低抑制了HCC的增殖和迁移。展开更多
Objective:To investigate the effects of serum from patients with pemphigus vulgaris(PV)on the transcription and protein expression level of calcium-transporting ATPase type 2C member 1(ATP2C1)and plakophilin 3(PKP3)in...Objective:To investigate the effects of serum from patients with pemphigus vulgaris(PV)on the transcription and protein expression level of calcium-transporting ATPase type 2C member 1(ATP2C1)and plakophilin 3(PKP3)in HaCaT cells.Methods:The HaCaT cells were divided into four groups:PV sera group,anti-Dsg3 monoclonal antibody group(AK23,positive control group),normal healthy serum group,and blank cell group.The groups were treated with corresponding different conditions for 24 hours.Quantitative polymerase chain reaction and Western blot were used to detect mRNA and protein levels of ATP2C1 and PKP3.Results:Compared with the blank group,the mRNA level of theATP2C1 and PKP3 genes in PV sera group was significantly increased by 384%and 404%,respectively(bothP<0.001).The treatment of PV sera and anti-Dsg3 antibody increased PKP3 protein expression(P=0.03 andP=0.004)but decreased protein expression of ATP2C1 in HaCaT cells(bothP<0.001).Conclusions:Our study indicates that serum from patients with PV promotes bothATP2C1 andPKP3 transcription in HaCaT cells,implying that the two genes may be involved in the pathological process of PV.展开更多
Hailey- Hailey disease (HHD) is an autosomal dominant disorder with recurrent eruption of vesicles and bullae involving predominantly the neck, groin and axillary regions. Histopathology shows suprabasal cleavage in e...Hailey- Hailey disease (HHD) is an autosomal dominant disorder with recurrent eruption of vesicles and bullae involving predominantly the neck, groin and axillary regions. Histopathology shows suprabasal cleavage in epidermal cells. Recent studies have revealed that HHD is caused by mutations in the ATP2C1 gene encoding a novel Ca2+ pump. We analyzed mutations of the ATP2C1 gene in 2 Japanese patients with HHD. The diagnosis of HHD was made based on the characteristic clinical features and histopathological evidence. All 27 exons and flanking intron boundaries were amplified by polymerase chain reaction and products analyzed by sequencing. As a result, we identified a novel missense mutation (A1087G) in exon 13 of the ATP2C1 gene in a patient. This mutation led the amino acid change from Thr to Ala in the phosphorylation protein domain. Another patient showed no mutation of the gene. These results demonstrate that a spectrum of ATP2C1 gene mutations is present in Japanese HHD patients.展开更多
Benign familial chronic pemphigus (Hailey-Hailey disease,HHD) is a rare hereditary condition characterized by development of blisters at sites of friction and in the intertriginous areas. Mutations in the ATP2C1 gene,...Benign familial chronic pemphigus (Hailey-Hailey disease,HHD) is a rare hereditary condition characterized by development of blisters at sites of friction and in the intertriginous areas. Mutations in the ATP2C1 gene, which encodes the human secretory pathway calcium ATPase 1 (hSPCA1), have been identified as possible causative mutations. Studying Hungarian patients with HHD, we found two novel, distinct, heterozygous mutations. In a 65-year-old man with a 41-year history of severe recurrent symptoms, a single nucleotide insertion,1085insA, was detected. In a patient whose symptoms were induced by environmental contact allergens, we found a nonsense mutation, Q506X, in exon 17. Our study further illustrates the diversity of mutational events in the pathogenesis of HHD.展开更多
Hailey-Hailey disease(HHD;MIM 16960)is a rare autosomal dominant hereditary disorder characterized by recurrent eruption of vesicles and bullae,predominantly involving the body folds.It is caused by heterozygous mutat...Hailey-Hailey disease(HHD;MIM 16960)is a rare autosomal dominant hereditary disorder characterized by recurrent eruption of vesicles and bullae,predominantly involving the body folds.It is caused by heterozygous mutations in the ATP2C1 gene,encoding the human secretory pathway Ca2+/Mn-ATPase protein 1(hSPCA1).When we studied Chinese patients with HHD,we found two different heterozygous mutations,Q506X and G353V,the former previously reported ina Hungarian patient,and the latter being a novel mutation.In a 38-year-old patient from a four-generation pedigree with a3-year history of severe recurrent blisters,we identified a C →T transition at nucleotide 1696,c(1696C →T),in exon 17 of ATP2C1,resulting in a nonsenes mutation,Gln506X,which resultedin a premature termination codon.In the second patient,who represented a occurrence of sporadic Hailey-Hailey disease,a G →T transversion of nucleotide,c(G1238T),in exon13 of ATP2C1 was detected,which resulted in a Gly353 →Val amino acid substitution(G353V).Our molecular findings further demonstrate that the mutational events in the human ATP2C1 gene encoding the hSPCA1 pump play an important role in the pathogenesis of HHD.展开更多
基金Supported by The Agricultural Seed Improvement Project of Shandong (2019LZGC017, 2019LZGC003)Agricultural Industry Research System (Rice) Project of Shandong Province (SDAIT-17-03)The Agricultural and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2021A30)。
文摘[Objective] The paper was to improve the blast resistance of insect-resistant transgenic rice. [Method] The Japonica rice variety Jikang10, a new transgenic variety with exogenous insect-resistant gene Cry1C, was used as the receptor, and Kongyu 131, a traditional breeding variety with broad-spectrum high blast resistance genes Pi1 and Pi2, was used as the donor to breeding new rice varieties. The genes were polymerized by hybridization and multi-generation backcrossing, and the offspring of each generation was screened by molecular marker assisted selection, field identification of multi-resistance against insect pests and diseases and agronomic trait selection. [Result] Four lines SK01, SK02, SK03 and SK04 with better resistances to insect pests and blast and outstanding agronomic traits in field were selected. [Conclusion] The results will lay foundations for breeding new multi-resistance rice varieties in Huanghuai rice region.
文摘Summary： The ATP2C1 gene mutation in one ease of familial benign chronic pemphigus was investigated.One patient was diagnosed as familial benign chronic pemphigus by pathology, ultrastructral examination and clinical features. Genomic DNA was extracted from blood samples. Mutation of ATP2CI gene was detected by polymerase chain reaction （PCR） and DNA sequencing. The results showed that deletion mutation was detected in ATP2C1 gene in this patient, which was 2374delTTTG. No mutation was found in the family members and normal individuals. It was coneluded that the 2374delTTTG mutation in ATP2C1 gene was the specific mutation for the clinical phenotype for this patient and was a de novo mutation.
文摘缺氧诱导因子(hypoxia-inducible factor, HIF)与肝细胞癌的发生发展相关。HIF-1α在包括肝细胞癌在内的多种癌症类型的发生发展中发挥着重要作用,但其在肝细胞癌中的靶基因尚未完全确定。为找到HIF-1α在肝癌中新的致癌靶点,通过整合HIF-1α敲除的RNA-seq数据,HIF-1α的ChIP-Seq数据,HIF-1α在肝癌中的共表达基因,以及肝癌相关的GEO(Gene Expression Omnibus)数据集,寻找HIF-1α的潜在靶基因。通过分析TCGA(The Cancer Genome Atlas)肝癌数据库、GEO和HPA(Human Protein Atlas)数据集,研究HIF-1α与ATP2C1的相关性,ATP2C1在肝癌中的表达及预后。通过建立物理和化学(氯化钴)缺氧模型验证ATP2C1与低氧及HIF-1α的关系。通过GO(Gene Ontology),KEGG(Kyoto Encyclopedia of Genes and Genomes)和GSEA(Gene Set Enrichment Analysis)分析探索ATP2C1的生物学功能。通过设计体外实验证实ATP2C1对HCC的作用。利用STRING和BioGRID两个蛋白互作在线数据库获得ATP2C1的互作蛋白,并研究其在肝癌中的表达及相关性。通过整合及筛选数据,ATP2C1被鉴定为一个HIF-1α的潜在靶基因。ATP2C1与HIF-1α高度相关,在肝细胞癌中高表达,且伴随有不良预后。富集分析与体外实验的结果表明ATP2C1参与调控HCC细胞的增殖迁移。蛋白互作数据表明ATP2C1与TMEM165存在互作关系,生存分析表明TMEM1651高表达的肝癌患者预后较差。相关性分析的结果显示ATP2C1与肝癌中TMEM165和MMP2的表达高度相关,表明ATP2C1可能与TMEM165和MMP2存在互作关系,并参与了肝癌的进展过程。结果表明,ATP2C1是HIF-1α的靶基因和肝细胞癌的生物标志物,其敲低抑制了HCC的增殖和迁移。
基金The study was supported by Scientific Research Plan from Guangzhou Science and Technology Bureau(No.201904010352)。
文摘Objective:To investigate the effects of serum from patients with pemphigus vulgaris(PV)on the transcription and protein expression level of calcium-transporting ATPase type 2C member 1(ATP2C1)and plakophilin 3(PKP3)in HaCaT cells.Methods:The HaCaT cells were divided into four groups:PV sera group,anti-Dsg3 monoclonal antibody group(AK23,positive control group),normal healthy serum group,and blank cell group.The groups were treated with corresponding different conditions for 24 hours.Quantitative polymerase chain reaction and Western blot were used to detect mRNA and protein levels of ATP2C1 and PKP3.Results:Compared with the blank group,the mRNA level of theATP2C1 and PKP3 genes in PV sera group was significantly increased by 384%and 404%,respectively(bothP<0.001).The treatment of PV sera and anti-Dsg3 antibody increased PKP3 protein expression(P=0.03 andP=0.004)but decreased protein expression of ATP2C1 in HaCaT cells(bothP<0.001).Conclusions:Our study indicates that serum from patients with PV promotes bothATP2C1 andPKP3 transcription in HaCaT cells,implying that the two genes may be involved in the pathological process of PV.
文摘Hailey- Hailey disease (HHD) is an autosomal dominant disorder with recurrent eruption of vesicles and bullae involving predominantly the neck, groin and axillary regions. Histopathology shows suprabasal cleavage in epidermal cells. Recent studies have revealed that HHD is caused by mutations in the ATP2C1 gene encoding a novel Ca2+ pump. We analyzed mutations of the ATP2C1 gene in 2 Japanese patients with HHD. The diagnosis of HHD was made based on the characteristic clinical features and histopathological evidence. All 27 exons and flanking intron boundaries were amplified by polymerase chain reaction and products analyzed by sequencing. As a result, we identified a novel missense mutation (A1087G) in exon 13 of the ATP2C1 gene in a patient. This mutation led the amino acid change from Thr to Ala in the phosphorylation protein domain. Another patient showed no mutation of the gene. These results demonstrate that a spectrum of ATP2C1 gene mutations is present in Japanese HHD patients.
文摘Benign familial chronic pemphigus (Hailey-Hailey disease,HHD) is a rare hereditary condition characterized by development of blisters at sites of friction and in the intertriginous areas. Mutations in the ATP2C1 gene, which encodes the human secretory pathway calcium ATPase 1 (hSPCA1), have been identified as possible causative mutations. Studying Hungarian patients with HHD, we found two novel, distinct, heterozygous mutations. In a 65-year-old man with a 41-year history of severe recurrent symptoms, a single nucleotide insertion,1085insA, was detected. In a patient whose symptoms were induced by environmental contact allergens, we found a nonsense mutation, Q506X, in exon 17. Our study further illustrates the diversity of mutational events in the pathogenesis of HHD.
文摘Hailey-Hailey disease(HHD;MIM 16960)is a rare autosomal dominant hereditary disorder characterized by recurrent eruption of vesicles and bullae,predominantly involving the body folds.It is caused by heterozygous mutations in the ATP2C1 gene,encoding the human secretory pathway Ca2+/Mn-ATPase protein 1(hSPCA1).When we studied Chinese patients with HHD,we found two different heterozygous mutations,Q506X and G353V,the former previously reported ina Hungarian patient,and the latter being a novel mutation.In a 38-year-old patient from a four-generation pedigree with a3-year history of severe recurrent blisters,we identified a C →T transition at nucleotide 1696,c(1696C →T),in exon 17 of ATP2C1,resulting in a nonsenes mutation,Gln506X,which resultedin a premature termination codon.In the second patient,who represented a occurrence of sporadic Hailey-Hailey disease,a G →T transversion of nucleotide,c(G1238T),in exon13 of ATP2C1 was detected,which resulted in a Gly353 →Val amino acid substitution(G353V).Our molecular findings further demonstrate that the mutational events in the human ATP2C1 gene encoding the hSPCA1 pump play an important role in the pathogenesis of HHD.
