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Genetic diversity analysis of Lepidium sativum(Chandrasur) using inter simple sequence repeat(ISSR) markers
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作者 Amandeep Kaur Rakesh Kumar +1 位作者 Suman Rani Anita Grewal 《Journal of Forestry Research》 SCIE CAS CSCD 2015年第1期107-114,共8页
Lepidium sativum(commonly known as garden cress) belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,... Lepidium sativum(commonly known as garden cress) belongs to the family Brassicaceae. It is a fastgrowing erect, annual herbaceous plant. Its seeds possess significant fracture healing, anti-asthmatic, anti-diabetic,hypoglycemic, nephrocurative and nephroprotective activities. In the present study, we assessed the genetic diversity of various genotypes of L. sativum using inter-simple sequence repeat(ISSR) markers. Out of 41 ISSR primers screened, 32 primers showed significant, clear and reproducible bands. A total of 510 amplified bands were obtained using 32 ISSR primers, out of which 422 bands were polymorphic and 88 bands were monomorphic. The percentage of polymorphism was found to be 82. A total of 35 unique alleles ranging insize from 200 to 2,900 bp were observed.Cluster analysis based on unweighted pair-group method,arithmetic mean divided the 18 genotypes into two main clusters, with the first having only HCS-08 genotype of L.sativum and other having all of the other 17 genotypes. The Jaccard similarity coefficient revealed a broad range32–72 % genetic relatedness among the 18 genotypes. 展开更多
关键词 ISSR genetic diversity Polymorphism Lepidium sativum cluster analysis
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Genetic climatic regionalization of the Balkan Peninsula using cluster analysis 被引量:2
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作者 Peter NOJAROV 《Journal of Geographical Sciences》 SCIE CSCD 2017年第1期43-61,共19页
Sea Level Pressure(SLP) data for the period 1950–2012 at 61 stations located in or around the Balkan Peninsula was used. The main concept is that intra-annual course of SLP represents the best different air masses ... Sea Level Pressure(SLP) data for the period 1950–2012 at 61 stations located in or around the Balkan Peninsula was used. The main concept is that intra-annual course of SLP represents the best different air masses that are situated over the Balkan Peninsula during the year. The method for differentiation of climatic zones is cluster analysis. A hierarchical clustering technique–average linkage between groups with Pearson correlation for measurement of intervals was employed in the research. The climate of the Balkan Peninsula is transitional between oceanic and continental and also between subtropical and temperate climates. Several major changes in atmospheric circulation over the Balkan Peninsula have happened over the period 1950–2012. There is a serious increase of the influence of the Azores High in the period January–Marchwhich leads to an increase of SLP and enhances oceanic influence. There is an increase of the influence of the north-west extension of the monsoonal low in the period June–September. This leads to more continental climatebut also to more tropical air masses over the Balkan Peninsula. Accordinglythe extent of subtropical climate widens in northern direction. There is an increase of the influence of the Siberian High in the period October–December. This influence covers central and eastern part of the peninsula in October and Novemberand it reaches western parts in December. Thusthe climate becomes more continental. 展开更多
关键词 genetic classification regionalization Balkan Peninsula cluster analysis Sea Level Pressure circulation indices
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Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding 被引量:5
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作者 Jun-mei WANG Jian-ming YANG Jing-huan ZHU Qiao-jun JIA Yue-zhi TAO 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2010年第10期792-800,共9页
The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the bad... The genetic diversity and relationship among 40 elite barley varieties were analyzed based on simple sequence repeat (SSR) genotyping data. The amplified fragments from SSR primers were highly polymorphic in the badey accessions investigated. A total of 85 alleles were detected at 35 SSR loci, and allelic variations existed at 29 SSR loci. The allele number per locus ranged from 1 to 5 with an average of 2.4 alleles per locus detected from the 40 badey accessions. A cluster analysis based on the genetic similarity coefficients was conducted and the 40 varieties were classified into two groups. Seven malting barley varieties from China fell into the same subgroup. It was found that the genetic diversity within the Chinese malting barley varieties was narrower than that in other barley germplasm sources, suggesting the importance and feasibility of introducing elite genotypes from different origins for malting barley breeding in China. 展开更多
关键词 Barley (Hordeum vu/gare L.) genetic similarity Simple sequence repeat (SSR) marker cluster analysis genetic diversity
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