目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN...目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN-γ、anti-IL-4抗体及IL-6、TGF-β1、IL-23,地塞米松+Th17组在Th17组培养条件下加10μg/L地塞米松。细胞培养72 h后,采用Real-time PCR检测4组IL-17f、gfi-1 m RNA表达。结果 Th17组IL-17f m RNA表达明显增高(P<0.01)。与Th17组比较,地塞米松+Th17组IL-17f m RNA表达明显降低(P<0.01),而gfi-1 m RNA表达明显增高(P<0.01)。结论地塞米松可下调IL-17f和上调gfi-1表达,gfi-1表达增加可能引起骨质疏松。展开更多
Naive CD4 T cells can differentiate into at least two different types ofT helpers, Thl and Th2 cells. Th2 cells, capable of producing IL-4, IL-5 and IL-13, are involved in humoral immunity against extracellular pathog...Naive CD4 T cells can differentiate into at least two different types ofT helpers, Thl and Th2 cells. Th2 cells, capable of producing IL-4, IL-5 and IL-13, are involved in humoral immunity against extracellular pathogens and in the induction of asthma and other allergic diseases. In this review, we summarize recent reports regarding the transcription factors involved in Th2 differentiation and cell expansion, including StatS, Gfi- 1 and GATA-3. Stats activation is necessary and sufficient for IL-2-mediated function in Th2 differentiation. Enhanced Stats signaling induces Th2 differentiation independent of IL-4 signaling; although it does not up-regulate GATA-3 expression, it does require the presence of GATA-3 for its action. Gfi-1, induced by IL-4, promotes the expansion of GATA-3-expressing cells. Analysis of conditional Gata3 knockout mice confirmed the critical role of GATA-3 in Th2 cell differentiation （both IL-4 dependent and IL-4 independent） and in Th2 cell proliferation and also showed the importance of basal GATA-3 expression in inhibiting Thl differentiation.展开更多
文摘目的研究地塞米松对小鼠Th17细胞分化模型中IL-17f、gfi-1表达的影响。方法提取小鼠脾脏淋巴细胞,采用免疫磁珠纯化CD4^+CD62L^+T细胞,将其分为对照组、地塞米松组、Th17组、地塞米松组+Th17组。Th17组加antiCD3ε、anti-CD28、anti-IFN-γ、anti-IL-4抗体及IL-6、TGF-β1、IL-23,地塞米松+Th17组在Th17组培养条件下加10μg/L地塞米松。细胞培养72 h后,采用Real-time PCR检测4组IL-17f、gfi-1 m RNA表达。结果 Th17组IL-17f m RNA表达明显增高(P<0.01)。与Th17组比较,地塞米松+Th17组IL-17f m RNA表达明显降低(P<0.01),而gfi-1 m RNA表达明显增高(P<0.01)。结论地塞米松可下调IL-17f和上调gfi-1表达,gfi-1表达增加可能引起骨质疏松。
文摘Naive CD4 T cells can differentiate into at least two different types ofT helpers, Thl and Th2 cells. Th2 cells, capable of producing IL-4, IL-5 and IL-13, are involved in humoral immunity against extracellular pathogens and in the induction of asthma and other allergic diseases. In this review, we summarize recent reports regarding the transcription factors involved in Th2 differentiation and cell expansion, including StatS, Gfi- 1 and GATA-3. Stats activation is necessary and sufficient for IL-2-mediated function in Th2 differentiation. Enhanced Stats signaling induces Th2 differentiation independent of IL-4 signaling; although it does not up-regulate GATA-3 expression, it does require the presence of GATA-3 for its action. Gfi-1, induced by IL-4, promotes the expansion of GATA-3-expressing cells. Analysis of conditional Gata3 knockout mice confirmed the critical role of GATA-3 in Th2 cell differentiation （both IL-4 dependent and IL-4 independent） and in Th2 cell proliferation and also showed the importance of basal GATA-3 expression in inhibiting Thl differentiation.
